P18104 (ANFC_PIG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
C-type natriuretic peptide

Cleaved into the following 3 chains:

1. CNP-22
2. CNP-29
3. CNP-53

Gene names

Name:	NPPC
Synonyms:	CNP

Organism

Sus scrofa (Pig) [Reference proteome]

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	126 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hormone which plays a role in endochondral ossification through regulation of cartilaginous growth plate chondrocytes proliferation and differentiation. May also be vasoactive and natriuretic. Specifically binds and stimulates the cGMP production of the NPR2 receptor. Binds the clearance receptor NPR3 By similarity.
Subcellular location	Secreted.
Sequence similarities	Belongs to the natriuretic peptide family.

Ontologies

Keywords
Biological process	Osteogenesis
Cellular component	Secreted
Domain	Signal
Molecular function	Hormone Vasoactive
PTM	Cleavage on pair of basic residues Disulfide bond
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cGMP biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB growth plate cartilage chondrocyte differentiation Inferred from sequence or structural similarity. Source: UniProtKB growth plate cartilage chondrocyte proliferation Inferred from sequence or structural similarity. Source: UniProtKB ossification Inferred from electronic annotation. Source: UniProtKB-KW post-embryonic development Inferred from electronic annotation. Source: Compara receptor guanylyl cyclase signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB regulation of blood vessel size Inferred from electronic annotation. Source: UniProtKB-KW regulation of multicellular organism growth Inferred from electronic annotation. Source: Compara
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	receptor binding Inferred from sequence or structural similarity. Source: AgBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Propeptide	24 – 73	50			PRO_0000001561
	Peptide	74 – 126	53	CNP-53 Ref.2		PRO_0000001562
	Peptide	98 – 126	29	CNP-29 By similarity		PRO_0000001563
	Peptide	105 – 126	22	CNP-22 Ref.3		PRO_0000001564
Amino acid modifications
	Disulfide bond	110 ↔ 126

Sequences

	Sequence		Length	Mass (Da)
	P18104 [UniParc]. Last modified August 1, 1991. Version 2. Checksum: E2474B2D4AABF4DD	FASTA	126	13,243
	10 20 30 40 50 60 MHLSQLLACA LLLTLLSLRP SEAKPGAPPK VPRTPPGEEV AEPQAAGGGQ KKGDKTPGGG 70 80 90 100 110 120 GANLKGDRSR LLRDLRVDTK SRAAWARLLH EHPNARKYKG GNKKGLSKGC FGLKLDRIGS MSGLGC

References

[1]	"Gene and precursor structure of porcine C-type natriuretic peptide." Tawaragi Y., Fuchimura K., Nakazato H., Tanaka S., Minamino N., Kangawa K., Matsuo H. Biochem. Biophys. Res. Commun. 172:627-632(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"N-terminally extended form of C-type natriuretic peptide (CNP-53) identified in porcine brain." Minamino N., Kangawa K., Matsuo H. Biochem. Biophys. Res. Commun. 170:973-979(1990) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 74-126. Tissue: Brain.
[3]	"C-type natriuretic peptide (CNP): a new member of natriuretic peptide family identified in porcine brain." Sudoh T., Minamino N., Kangawa K., Matsuo H. Biochem. Biophys. Res. Commun. 168:863-870(1990) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 105-126. Tissue: Brain.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M64758 Genomic DNA. Translation: AAA31018.1.
PIR	A36155.
RefSeq	NP_001008482.1. NM_001008482.1.
UniGene	Ssc.23867.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSSSCT00000028969; ENSSSCP00000022217; ENSSSCG00000026852.
GeneID	493772.
KEGG	ssc:493772.
Organism-specific databases
CTD	4880.
Phylogenomic databases
GeneTree	ENSGT00390000015492.
HOVERGEN	HBG004229.
KO	K12336.
OMA	HKMDRIG.
Family and domain databases
InterPro	IPR002406. C_natriurtcpep. IPR000663. Natr_peptide. [Graphical view]
Pfam	PF00212. ANP. 1 hit. [Graphical view]
PRINTS	PR00713. CNATPEPTIDE. PR00710. NATPEPTIDES.
SMART	SM00183. NAT_PEP. 1 hit. [Graphical view]
PROSITE	PS00263. NATRIURETIC_PEPTIDE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
PMAP-CutDB	P18104.

Entry information

Entry name

ANFC_PIG

Accession

Primary (citable) accession number: P18104
Secondary accession number(s): P21806

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	August 1, 1991
Last modified:	April 3, 2013
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program