Reviewed, UniProtKB/Swiss-Prot P18102 (TLL_DROME)

Names and origin

Protein names

Recommended name:
    Protein tailless
Alternative name(s):
    Nuclear receptor subfamily 2 group E member 2

Gene names

Name:	tll
Synonyms:	NR2E2
ORF Names:	CG1378

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	452 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Orphan receptor that binds DNA as a monomer to hormone response elements (HRE) containing an extended core motif half-site sequence 5'-AAGTCA-3' in which the 5' flanking nucleotides participate in determining receptor specificity. This receptor binds to the consensus sequence [AG][AG]AAGTCAA. Plays a key role in the establishment of non-metameric domains at the anterior and posterior poles of the embryo. It may also play a role in the nervous system. The maternal terminal pathway activates the tll gene in the termini; TLL activity then represses segmentation and activates terminal-specific genes in these domains. Involved in the regulation of early eye development. In the embryonic visual system anlage drives cells to optic lobe as opposed to Bolwig's organ fate. Ref.1 Ref.6
Subunit structure	Monomer By similarity.
Subcellular location	Nucleus Potential.
Tissue specificity	Brain and peripheral nervous system. Ref.1
Developmental stage	During stage 10 found in the anterior part of the visual system that later gives rise to the anterior lip of the optic lobe. At stage 12 also found in the posterior lip of the optic lobe. In third larval instar expressed in the optic lobe of the larval brain and in the eye antennal disk, both in antennal and eye portion. Ref.1
Sequence similarities	Belongs to the nuclear hormone receptor family. NR2 subfamily. Contains 1 nuclear receptor DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator Developmental protein Receptor Repressor
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Bolwig's organ morphogenesis Traceable author statement. Source: FlyBase cell fate specification Traceable author statement. Source: FlyBase gastrulation Non-traceable author statement. Source: FlyBase optic lobe placode development Ref.6 Inferred from mutant phenotype. Source: FlyBase regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW ring gland development Inferred from mutant phenotype. Source: FlyBase terminal region determination Inferred from genetic interaction. Source: FlyBase torso signaling pathway Inferred from genetic interaction. Source: FlyBase transcription, DNA-dependent Non-traceable author statement. Source: FlyBase
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro steroid hormone receptor activity Inferred from electronic annotation. Source: InterPro transcription factor activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q8IMJ2	1	EBI-159966,EBI-116573
	Q9VIV7	1	EBI-159966,EBI-151022
	Q9W3Q4	1	EBI-159966,EBI-178344

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 452	452	Protein tailless		PRO_0000053597
Regions
	DNA binding	31 – 108	78	Nuclear receptor
	Zinc finger	34 – 54	21	NR C4-type
	Zinc finger	70 – 96	27	NR C4-type
	Region	244 – 389	146	Ligand-binding By similarity
	Compositional bias	262 – 265	4	Poly-Leu

Sequences

	Sequence		Length	Mass (Da)
	P18102-1 [UniParc]. Last modified November 1, 1990. Version 1. Checksum: A4ABEFFDE993A37C	FASTA	452	50,549
	10 20 30 40 50 60 MQSSEGSPDM MDQKYNSVRL SPAASSRILY HVPCKVCRDH SSGKHYGIYA CDGCAGFFKR 70 80 90 100 110 120 SIRRSRQYVC KSQKQGLCVV DKTHRNQCRA CRLRKCFEVG MNKDAVQHER GPRNSTLRRH 130 140 150 160 170 180 MAMYKDAMMG AGEMPQIPAE ILMNTAALTG FPGVPMPMPG LPQRAGHHPA HMAAFQPPPS 190 200 210 220 230 240 AAAVLDLSVP RVPHHPVHQG HHGFFSPTAA YMNALATRAL PPTPPLMAAE HIKETAAEHL 250 260 270 280 290 300 FKNVNWIKSV RAFTELPMPD QLLLLEESWK EFFILAMAQY LMPMNFAQLL FVYESENANR 310 320 330 340 350 360 EIMGMVTREV HAFQEVLNQL CHLNIDSTEY ECLRAISLFR KSPPSASSTE DLANSSILTG 370 380 390 400 410 420 SGSPNSSASA ESRGLLESGK VAAMHNDARS ALHNYIQRTH PSQPMRFQTL LGVVQLMHKV 430 440 450 SSFTIEELFF RKTIGDITIV RLISDMYSQR KI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Drosophila gene tailless is expressed at the embryonic termini and is a member of the steroid receptor superfamily." Pignoni F., Baldarelli R.M., Steingrimsson E., Diaz R.J., Patapoutian A., Merriam J.R., Lengyel J.A. Cell 62:151-163(1990) [PubMed: 2364433] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Strain: Oregon-R.
[2]	"Characterization of downstream elements in a Raf-1 pathway." Liaw G.-J., Steingrimsson E., Pignoni F., Courey A.J., Lengyel J.A. Proc. Natl. Acad. Sci. U.S.A. 90:858-862(1993) [PubMed: 8430097] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[5]	Stapleton M., Carlson J.W., Frise E., Kapadia B., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley.
[6]	"The control of cell fate in the embryonic visual system by atonal, tailless and EGFR signaling." Daniel A., Dumstrei K., Lengyel J.A., Hartenstein V. Development 126:2945-2954(1999) [PubMed: 10357938] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M34639 mRNA. Translation: AAA28936.1. AF019362 Genomic DNA. Translation: AAB71371.1. AE014297 Genomic DNA. Translation: AAF57091.1. BT022195 mRNA. Translation: AAY51589.1.
PIR	A35602.
RefSeq	NP_524596.1.
UniGene	Dm.1906
3D structure databases
HSSP	HSSP built from PDB template 1HRA based on UniProtKB P10826.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:19302N.
IntAct	P18102. 14 interactions.
Proteomic databases
PRIDE	P18102.
Genome annotation databases
Ensembl	FBgn0003720. Drosophila melanogaster. [Contig view]
GeneID	43656.
KEGG	dme:Dmel_CG1378.
NMPDR	fig|7227.3.peg.15514.
Organism-specific databases
FlyBase	FBgn0003720. tll.
Phylogenomic databases
HOGENOM	P18102.
OMA	P18102. QLEPHNL.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-014241-MON.
Gene expression databases
ArrayExpress	P18102.
GermOnline	CG1378. Drosophila melanogaster.
Family and domain databases
InterPro	IPR008946. Nucl_hormone_rcpt_ligand-bd. IPR000536. Nucl_hrmn_rcpt_lig-bd_core. IPR001723. Str_hrmn_rcpt. IPR000324. VitD_rcpt. IPR001628. Znf_hrmn_rcpt. IPR013088. Znf_NHR/GATA. [Graphical view]
Gene3D	G3DSA:1.10.565.10. Nucl_hrmn_rcpt_lig_bd. 1 hit. G3DSA:3.30.50.10. Znf_NHR/GATA. 1 hit.
Pfam	PF00104. Hormone_recep. 1 hit. PF00105. zf-C4. 1 hit. [Graphical view]
PRINTS	PR00398. STRDHORMONER. PR00047. STROIDFINGER. PR00350. VITAMINDR.
ProDom	PD000035. Znf_C4steroid. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00430. HOLI. 1 hit. SM00399. ZnF_C4. 1 hit. [Graphical view]
PROSITE	PS00031. NUCLEAR_REC_DBD_1. 1 hit. PS51030. NUCLEAR_REC_DBD_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	835100.

Entry information

Entry name

TLL_DROME

Accession

Primary (citable) accession number: P18102
Secondary accession number(s): Q4V6W1, Q9VA33

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	June 16, 2009
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project