Reviewed, UniProtKB/Swiss-Prot P18085 (ARF4_HUMAN)

Names and origin

Protein names

Recommended name:
    ADP-ribosylation factor 4

Gene names

Name:	ARF4
Synonyms:	ARF2

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	180 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.
Subcellular location	Golgi apparatus.
Sequence similarities	Belongs to the small GTPase superfamily. Arf family.
Caution	Was originally thought to be ARF2.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Golgi apparatus
Coding sequence diversity	Polymorphism
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Myristate
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro vesicle-mediated transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Potential

Chain

2 – 180

179

ADP-ribosylation factor 4

PRO_0000207391

Regions

Nucleotide binding

24 – 31

8

GTP

Nucleotide binding

67 – 71

5

GTP

Nucleotide binding

126 – 129

4

GTP

Amino acid modifications

Lipidation

2

1

N-myristoyl glycine Potential

Natural variations

Natural variant

68

1

V → A: dbSNP rs11550597.

VAR_048317

Secondary structure

1

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180

Helix Strand Turn

Details...

Helix

5 – 8

4

Turn

9 – 12

4

Beta strand

14 – 16

3

Beta strand

18 – 25

8

Helix

30 – 37

8

Beta strand

43 – 48

6

Beta strand

51 – 57

7

Beta strand

59 – 67

9

Beta strand

69 – 71

3

Helix

77 – 80

4

Beta strand

83 – 95

13

Helix

97 – 99

3

Helix

100 – 112

13

Helix

114 – 116

3

Beta strand

120 – 126

7

Helix

136 – 143

8

Helix

145 – 147

3

Beta strand

153 – 157

5

Helix

160 – 162

3

Helix

166 – 177

12

Sequences

	Sequence		Length	Mass (Da)
	P18085-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 3A43EB1F37A81BD9	FASTA	180	20,511
	10 20 30 40 50 60 MGLTISSLFS RLFGKKQMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG FNVETVEYKN 70 80 90 100 110 120 ICFTVWDVGG QDRIRPLWKH YFQNTQGLIF VVDSNDRERI QEVADELQKM LLVDELRDAV 130 140 150 160 170 180 LLLFANKQDL PNAMAISEMT DKLGLQSLRN RTWYVQATCA TQGTGLYEGL DWLSNELSKR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Selective amplification of an mRNA and related pseudogene for a human ADP-ribosylation factor, a guanine nucleotide-dependent protein activator of cholera toxin." Monaco L., Murtagh J.J. Jr., Newman K.B., Tsai S.-C., Moss J., Vaughan M. Proc. Natl. Acad. Sci. U.S.A. 87:2206-2210(1990) [PubMed: 2107548] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Human ADP-ribosylation factors. A functionally conserved family of GTP-binding proteins." Kahn R.A., Kern F.G., Clark J., Gelmann E.P., Rulka C. J. Biol. Chem. 266:2606-2614(1991) [PubMed: 1899243] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Cloning and characterization of the human ADP-ribosylation factor 4 gene." Lebeda R.A., Haun R.S. Gene 237:209-214(1999) [PubMed: 10524252] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Cerebellum.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Bone marrow, Placenta, Skin and Urinary bladder.
[7]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[8]	"Structure of human ADP-ribosylation factor 4." Structural genomics consortium (SGC) Submitted (APR-2005) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) IN COMPLEX WITH GDP.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M36341 mRNA. Translation: AAA53081.1.
AF104238 , AF104233, AF104234, AF104235, AF104236, AF104237 Genomic DNA. Translation: AAD54674.1.
AF493883 mRNA. Translation: AAM12597.1.
AK313008 mRNA. Translation: BAG35844.1.
BC003364 mRNA. Translation: AAH03364.1.
BC008753 mRNA. Translation: AAH08753.1.
BC016325 mRNA. Translation: AAH16325.1.
BC022866 mRNA. Translation: AAH22866.1.

IPI

IPI00215918.

PIR

B38622.

RefSeq

NP_001651.1.

UniGene

Hs.695943

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1Z6X	X-ray	2.70	A/B	2-179	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

P18085.

Proteomic databases

PRIDE

P18085.

Genome annotation databases

Ensembl

ENST00000303436; ENSP00000306010; ENSG00000168374; Homo sapiens. [Genome view]

GeneID

378.

KEGG

hsa:378.

UCSC

uc003dix.2. human.

Organism-specific databases

CTD

378.

GeneCards

GC03M057532.

H-InvDB

HIX0003394.

HGNC

HGNC:655. ARF4.

MIM

601177. gene.

PharmGKB

PA24937.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

P18085.

HOVERGEN

P18085.

OMA

TEAQDEL.

Gene expression databases

ArrayExpress

P18085.

Bgee

P18085.

CleanEx

HS_ARF4.

Genevestigator

P18085.

GermOnline

ENSG00000168374. Homo sapiens.

Family and domain databases

InterPro

IPR006688. ARF.
IPR006689. ARF/SAR.
IPR005225. Small_GTP_bd.
[Graphical view]

PANTHER

PTHR11711. ARF/SAR. 1 hit.

Pfam

PF00025. Arf. 1 hit.
[Graphical view]

PRINTS

PR00328. SAR1GTPBP.

SMART

SM00177. ARF. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00231. small_GTP. 1 hit.

PROSITE

PS51417. ARF. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

1583.

SOURCE

Search...

Entry information

Entry name

ARF4_HUMAN

Accession

Primary (citable) accession number: P18085
Secondary accession number(s): B2R7J7, P21371

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 108 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.