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Protein

ADP-ribosylation factor 4

Gene

ARF4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi24 – 31GTP8
Nucleotide bindingi67 – 71GTP5
Nucleotide bindingi126 – 129GTP4

GO - Molecular functioni

  • ARF guanyl-nucleotide exchange factor activity Source: Reactome
  • epidermal growth factor receptor binding Source: UniProtKB
  • GTPase activity Source: Reactome
  • GTP binding Source: UniProtKB

GO - Biological processi

  • activation of phospholipase D activity Source: UniProtKB
  • apical protein localization Source: Ensembl
  • brain development Source: Ensembl
  • cell migration Source: UniProtKB
  • dendritic spine development Source: MGI
  • epidermal growth factor receptor signaling pathway Source: Ensembl
  • ER to Golgi vesicle-mediated transport Source: Reactome
  • establishment or maintenance of epithelial cell apical/basal polarity Source: Ensembl
  • learning Source: Ensembl
  • negative regulation of apoptotic process Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • protein ADP-ribosylation Source: UniProtKB
  • protein localization to cilium Source: Ensembl
  • protein transport Source: UniProtKB-KW
  • regulation of reactive oxygen species metabolic process Source: UniProtKB
  • response to axon injury Source: Ensembl
  • retrograde vesicle-mediated transport, Golgi to ER Source: WormBase
  • small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000168374-MONOMER.
ReactomeiR-HSA-5620916. VxPx cargo-targeting to cilium.
R-HSA-6807878. COPI-mediated anterograde transport.
R-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor 4
Gene namesi
Name:ARF4
Synonyms:ARF2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:655. ARF4.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • dendritic spine Source: MGI
  • extracellular exosome Source: UniProtKB
  • extracellular matrix Source: BHF-UCL
  • Golgi apparatus Source: UniProtKB-SubCell
  • membrane Source: UniProtKB
  • ruffle membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi378.
OpenTargetsiENSG00000168374.
PharmGKBiPA24937.

Polymorphism and mutation databases

BioMutaiARF4.
DMDMi114123.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002073912 – 180ADP-ribosylation factor 4Add BLAST179

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine1 Publication1
Modified residuei147PhosphoserineCombined sources1

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

EPDiP18085.
MaxQBiP18085.
PaxDbiP18085.
PeptideAtlasiP18085.
PRIDEiP18085.
TopDownProteomicsiP18085.

PTM databases

iPTMnetiP18085.
PhosphoSitePlusiP18085.
SwissPalmiP18085.

Expressioni

Gene expression databases

BgeeiENSG00000168374.
CleanExiHS_ARF4.
ExpressionAtlasiP18085. baseline and differential.
GenevisibleiP18085. HS.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
RCHY1Q96PM53EBI-1237085,EBI-947779
SDCBP2Q9H1903EBI-1237085,EBI-742426

GO - Molecular functioni

  • epidermal growth factor receptor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi106873. 47 interactors.
DIPiDIP-38214N.
IntActiP18085. 12 interactors.
MINTiMINT-2801449.
STRINGi9606.ENSP00000306010.

Structurei

Secondary structure

1180
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 8Combined sources4
Turni9 – 12Combined sources4
Beta strandi14 – 16Combined sources3
Beta strandi18 – 25Combined sources8
Helixi30 – 37Combined sources8
Beta strandi42 – 48Combined sources7
Beta strandi51 – 57Combined sources7
Beta strandi59 – 67Combined sources9
Beta strandi69 – 71Combined sources3
Helixi77 – 80Combined sources4
Beta strandi83 – 95Combined sources13
Helixi97 – 99Combined sources3
Helixi100 – 112Combined sources13
Helixi114 – 116Combined sources3
Beta strandi120 – 126Combined sources7
Helixi136 – 143Combined sources8
Helixi145 – 147Combined sources3
Beta strandi153 – 157Combined sources5
Helixi160 – 162Combined sources3
Helixi166 – 177Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Z6XX-ray2.70A/B1-180[»]
ProteinModelPortaliP18085.
SMRiP18085.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP18085.

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiKOG0070. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00780000121855.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiP18085.
KOiK07939.
OMAiWASSEIF.
OrthoDBiEOG091G0S7S.
PhylomeDBiP18085.
TreeFamiTF300808.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P18085-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLTISSLFS RLFGKKQMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG
60 70 80 90 100
FNVETVEYKN ICFTVWDVGG QDRIRPLWKH YFQNTQGLIF VVDSNDRERI
110 120 130 140 150
QEVADELQKM LLVDELRDAV LLLFANKQDL PNAMAISEMT DKLGLQSLRN
160 170 180
RTWYVQATCA TQGTGLYEGL DWLSNELSKR
Length:180
Mass (Da):20,511
Last modified:January 23, 2007 - v3
Checksum:i3A43EB1F37A81BD9
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04831768V → A.Corresponds to variant rs11550597dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36341 mRNA. Translation: AAA53081.1.
AF104238
, AF104233, AF104234, AF104235, AF104236, AF104237 Genomic DNA. Translation: AAD54674.1.
AF493883 mRNA. Translation: AAM12597.1.
AK313008 mRNA. Translation: BAG35844.1.
BC003364 mRNA. Translation: AAH03364.1.
BC008753 mRNA. Translation: AAH08753.1.
BC016325 mRNA. Translation: AAH16325.1.
BC022866 mRNA. Translation: AAH22866.1.
CCDSiCCDS2884.1.
PIRiB38622.
RefSeqiNP_001651.1. NM_001660.3.
UniGeneiHs.652183.

Genome annotation databases

EnsembliENST00000303436; ENSP00000306010; ENSG00000168374.
GeneIDi378.
KEGGihsa:378.
UCSCiuc003dix.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36341 mRNA. Translation: AAA53081.1.
AF104238
, AF104233, AF104234, AF104235, AF104236, AF104237 Genomic DNA. Translation: AAD54674.1.
AF493883 mRNA. Translation: AAM12597.1.
AK313008 mRNA. Translation: BAG35844.1.
BC003364 mRNA. Translation: AAH03364.1.
BC008753 mRNA. Translation: AAH08753.1.
BC016325 mRNA. Translation: AAH16325.1.
BC022866 mRNA. Translation: AAH22866.1.
CCDSiCCDS2884.1.
PIRiB38622.
RefSeqiNP_001651.1. NM_001660.3.
UniGeneiHs.652183.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Z6XX-ray2.70A/B1-180[»]
ProteinModelPortaliP18085.
SMRiP18085.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106873. 47 interactors.
DIPiDIP-38214N.
IntActiP18085. 12 interactors.
MINTiMINT-2801449.
STRINGi9606.ENSP00000306010.

PTM databases

iPTMnetiP18085.
PhosphoSitePlusiP18085.
SwissPalmiP18085.

Polymorphism and mutation databases

BioMutaiARF4.
DMDMi114123.

Proteomic databases

EPDiP18085.
MaxQBiP18085.
PaxDbiP18085.
PeptideAtlasiP18085.
PRIDEiP18085.
TopDownProteomicsiP18085.

Protocols and materials databases

DNASUi378.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000303436; ENSP00000306010; ENSG00000168374.
GeneIDi378.
KEGGihsa:378.
UCSCiuc003dix.5. human.

Organism-specific databases

CTDi378.
DisGeNETi378.
GeneCardsiARF4.
HGNCiHGNC:655. ARF4.
MIMi601177. gene.
neXtProtiNX_P18085.
OpenTargetsiENSG00000168374.
PharmGKBiPA24937.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0070. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00780000121855.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiP18085.
KOiK07939.
OMAiWASSEIF.
OrthoDBiEOG091G0S7S.
PhylomeDBiP18085.
TreeFamiTF300808.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000168374-MONOMER.
ReactomeiR-HSA-5620916. VxPx cargo-targeting to cilium.
R-HSA-6807878. COPI-mediated anterograde transport.
R-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Miscellaneous databases

ChiTaRSiARF4. human.
EvolutionaryTraceiP18085.
GeneWikiiARF4.
GenomeRNAii378.
PROiP18085.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000168374.
CleanExiHS_ARF4.
ExpressionAtlasiP18085. baseline and differential.
GenevisibleiP18085. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARF4_HUMAN
AccessioniPrimary (citable) accession number: P18085
Secondary accession number(s): B2R7J7, P21371
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 179 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Was originally thought to be ARF2.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.