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Protein

ADP-ribosylation factor 4

Gene

ARF4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi24 – 318GTP
Nucleotide bindingi67 – 715GTP
Nucleotide bindingi126 – 1294GTP

GO - Molecular functioni

  1. epidermal growth factor receptor binding Source: UniProtKB
  2. GTPase activity Source: Reactome
  3. GTP binding Source: UniProtKB

GO - Biological processi

  1. activation of phospholipase D activity Source: UniProtKB
  2. brain development Source: Ensembl
  3. cell migration Source: UniProtKB
  4. epidermal growth factor receptor signaling pathway Source: Ensembl
  5. negative regulation of apoptotic process Source: UniProtKB
  6. organelle organization Source: Reactome
  7. positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  8. protein ADP-ribosylation Source: UniProtKB
  9. protein transport Source: UniProtKB-KW
  10. regulation of reactive oxygen species metabolic process Source: UniProtKB
  11. response to axon injury Source: Ensembl
  12. small GTPase mediated signal transduction Source: InterPro
  13. vesicle-mediated transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_268299. VxPx cargo-targeting to cilium.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor 4
Gene namesi
Name:ARF4
Synonyms:ARF2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:655. ARF4.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: UniProtKB
  2. extracellular vesicular exosome Source: UniProtKB
  3. Golgi apparatus Source: UniProtKB-SubCell
  4. membrane Source: UniProtKB
  5. ruffle membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA24937.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedSequence Analysis
Chaini2 – 180179ADP-ribosylation factor 4PRO_0000207391Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineSequence Analysis

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

MaxQBiP18085.
PaxDbiP18085.
PRIDEiP18085.

PTM databases

PhosphoSiteiP18085.

Expressioni

Gene expression databases

BgeeiP18085.
CleanExiHS_ARF4.
ExpressionAtlasiP18085. baseline and differential.
GenevestigatoriP18085.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
RCHY1Q96PM53EBI-1237085,EBI-947779

Protein-protein interaction databases

BioGridi106873. 22 interactions.
IntActiP18085. 7 interactions.
MINTiMINT-2801449.
STRINGi9606.ENSP00000306010.

Structurei

Secondary structure

1
180
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 84Combined sources
Turni9 – 124Combined sources
Beta strandi14 – 163Combined sources
Beta strandi18 – 258Combined sources
Helixi30 – 378Combined sources
Beta strandi42 – 487Combined sources
Beta strandi51 – 577Combined sources
Beta strandi59 – 679Combined sources
Beta strandi69 – 713Combined sources
Helixi77 – 804Combined sources
Beta strandi83 – 9513Combined sources
Helixi97 – 993Combined sources
Helixi100 – 11213Combined sources
Helixi114 – 1163Combined sources
Beta strandi120 – 1267Combined sources
Helixi136 – 1438Combined sources
Helixi145 – 1473Combined sources
Beta strandi153 – 1575Combined sources
Helixi160 – 1623Combined sources
Helixi166 – 17712Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Z6XX-ray2.70A/B1-180[»]
ProteinModelPortaliP18085.
SMRiP18085. Positions 4-178.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP18085.

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00780000121855.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiP18085.
KOiK07939.
OMAiWASSEIF.
OrthoDBiEOG77WWDV.
PhylomeDBiP18085.
TreeFamiTF300808.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SMARTiSM00177. ARF. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P18085-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLTISSLFS RLFGKKQMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG
60 70 80 90 100
FNVETVEYKN ICFTVWDVGG QDRIRPLWKH YFQNTQGLIF VVDSNDRERI
110 120 130 140 150
QEVADELQKM LLVDELRDAV LLLFANKQDL PNAMAISEMT DKLGLQSLRN
160 170 180
RTWYVQATCA TQGTGLYEGL DWLSNELSKR
Length:180
Mass (Da):20,511
Last modified:January 22, 2007 - v3
Checksum:i3A43EB1F37A81BD9
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti68 – 681V → A.
Corresponds to variant rs11550597 [ dbSNP | Ensembl ].
VAR_048317

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36341 mRNA. Translation: AAA53081.1.
AF104238
, AF104233, AF104234, AF104235, AF104236, AF104237 Genomic DNA. Translation: AAD54674.1.
AF493883 mRNA. Translation: AAM12597.1.
AK313008 mRNA. Translation: BAG35844.1.
BC003364 mRNA. Translation: AAH03364.1.
BC008753 mRNA. Translation: AAH08753.1.
BC016325 mRNA. Translation: AAH16325.1.
BC022866 mRNA. Translation: AAH22866.1.
CCDSiCCDS2884.1.
PIRiB38622.
RefSeqiNP_001651.1. NM_001660.3.
UniGeneiHs.652183.

Genome annotation databases

EnsembliENST00000303436; ENSP00000306010; ENSG00000168374.
GeneIDi378.
KEGGihsa:378.
UCSCiuc003dix.4. human.

Polymorphism databases

DMDMi114123.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36341 mRNA. Translation: AAA53081.1.
AF104238
, AF104233, AF104234, AF104235, AF104236, AF104237 Genomic DNA. Translation: AAD54674.1.
AF493883 mRNA. Translation: AAM12597.1.
AK313008 mRNA. Translation: BAG35844.1.
BC003364 mRNA. Translation: AAH03364.1.
BC008753 mRNA. Translation: AAH08753.1.
BC016325 mRNA. Translation: AAH16325.1.
BC022866 mRNA. Translation: AAH22866.1.
CCDSiCCDS2884.1.
PIRiB38622.
RefSeqiNP_001651.1. NM_001660.3.
UniGeneiHs.652183.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Z6XX-ray2.70A/B1-180[»]
ProteinModelPortaliP18085.
SMRiP18085. Positions 4-178.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106873. 22 interactions.
IntActiP18085. 7 interactions.
MINTiMINT-2801449.
STRINGi9606.ENSP00000306010.

PTM databases

PhosphoSiteiP18085.

Polymorphism databases

DMDMi114123.

Proteomic databases

MaxQBiP18085.
PaxDbiP18085.
PRIDEiP18085.

Protocols and materials databases

DNASUi378.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000303436; ENSP00000306010; ENSG00000168374.
GeneIDi378.
KEGGihsa:378.
UCSCiuc003dix.4. human.

Organism-specific databases

CTDi378.
GeneCardsiGC03M057559.
HGNCiHGNC:655. ARF4.
MIMi601177. gene.
neXtProtiNX_P18085.
PharmGKBiPA24937.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00780000121855.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiP18085.
KOiK07939.
OMAiWASSEIF.
OrthoDBiEOG77WWDV.
PhylomeDBiP18085.
TreeFamiTF300808.

Enzyme and pathway databases

ReactomeiREACT_268299. VxPx cargo-targeting to cilium.

Miscellaneous databases

ChiTaRSiARF4. human.
EvolutionaryTraceiP18085.
GeneWikiiARF4.
GenomeRNAii378.
NextBioi1583.
PROiP18085.
SOURCEiSearch...

Gene expression databases

BgeeiP18085.
CleanExiHS_ARF4.
ExpressionAtlasiP18085. baseline and differential.
GenevestigatoriP18085.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SMARTiSM00177. ARF. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Selective amplification of an mRNA and related pseudogene for a human ADP-ribosylation factor, a guanine nucleotide-dependent protein activator of cholera toxin."
    Monaco L., Murtagh J.J. Jr., Newman K.B., Tsai S.-C., Moss J., Vaughan M.
    Proc. Natl. Acad. Sci. U.S.A. 87:2206-2210(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Human ADP-ribosylation factors. A functionally conserved family of GTP-binding proteins."
    Kahn R.A., Kern F.G., Clark J., Gelmann E.P., Rulka C.
    J. Biol. Chem. 266:2606-2614(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Cloning and characterization of the human ADP-ribosylation factor 4 gene."
    Lebeda R.A., Haun R.S.
    Gene 237:209-214(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
    Puhl H.L. III, Ikeda S.R., Aronstam R.S.
    Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Cerebellum.
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Bone marrow, Placenta, Skin and Urinary bladder.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  9. "Structure of human ADP-ribosylation factor 4."
    Structural genomics consortium (SGC)
    Submitted (MAR-2005) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) IN COMPLEX WITH GDP.

Entry informationi

Entry nameiARF4_HUMAN
AccessioniPrimary (citable) accession number: P18085
Secondary accession number(s): B2R7J7, P21371
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1990
Last sequence update: January 22, 2007
Last modified: March 31, 2015
This is version 161 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Was originally thought to be ARF2.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.