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P18085

- ARF4_HUMAN

UniProt

P18085 - ARF4_HUMAN

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Protein

ADP-ribosylation factor 4

Gene

ARF4

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi24 – 318GTP
Nucleotide bindingi67 – 715GTP
Nucleotide bindingi126 – 1294GTP

GO - Molecular functioni

  1. epidermal growth factor receptor binding Source: UniProt
  2. GTP binding Source: UniProt

GO - Biological processi

  1. activation of phospholipase D activity Source: UniProt
  2. brain development Source: Ensembl
  3. cell migration Source: UniProt
  4. epidermal growth factor receptor signaling pathway Source: Ensembl
  5. negative regulation of apoptotic process Source: UniProt
  6. positive regulation of transcription from RNA polymerase II promoter Source: UniProt
  7. protein ADP-ribosylation Source: UniProt
  8. protein transport Source: UniProtKB-KW
  9. regulation of reactive oxygen species metabolic process Source: UniProt
  10. response to axon injury Source: Ensembl
  11. small GTPase mediated signal transduction Source: InterPro
  12. vesicle-mediated transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor 4
Gene namesi
Name:ARF4
Synonyms:ARF2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 3

Organism-specific databases

HGNCiHGNC:655. ARF4.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: UniProt
  2. extracellular vesicular exosome Source: UniProt
  3. Golgi apparatus Source: UniProtKB-KW
  4. membrane Source: UniProtKB
  5. ruffle membrane Source: UniProt
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA24937.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedSequence Analysis
Chaini2 – 180179ADP-ribosylation factor 4PRO_0000207391Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineSequence Analysis

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

MaxQBiP18085.
PaxDbiP18085.
PRIDEiP18085.

PTM databases

PhosphoSiteiP18085.

Expressioni

Gene expression databases

BgeeiP18085.
CleanExiHS_ARF4.
ExpressionAtlasiP18085. baseline and differential.
GenevestigatoriP18085.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
RCHY1Q96PM53EBI-1237085,EBI-947779

Protein-protein interaction databases

BioGridi106873. 22 interactions.
IntActiP18085. 7 interactions.
MINTiMINT-2801449.
STRINGi9606.ENSP00000306010.

Structurei

Secondary structure

1
180
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 84
Turni9 – 124
Beta strandi14 – 163
Beta strandi18 – 258
Helixi30 – 378
Beta strandi42 – 487
Beta strandi51 – 577
Beta strandi59 – 679
Beta strandi69 – 713
Helixi77 – 804
Beta strandi83 – 9513
Helixi97 – 993
Helixi100 – 11213
Helixi114 – 1163
Beta strandi120 – 1267
Helixi136 – 1438
Helixi145 – 1473
Beta strandi153 – 1575
Helixi160 – 1623
Helixi166 – 17712

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Z6XX-ray2.70A/B1-180[»]
ProteinModelPortaliP18085.
SMRiP18085. Positions 4-178.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP18085.

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00760000118835.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiP18085.
KOiK07939.
OMAiGADELQK.
OrthoDBiEOG77WWDV.
PhylomeDBiP18085.
TreeFamiTF300808.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SMARTiSM00177. ARF. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P18085-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGLTISSLFS RLFGKKQMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG
60 70 80 90 100
FNVETVEYKN ICFTVWDVGG QDRIRPLWKH YFQNTQGLIF VVDSNDRERI
110 120 130 140 150
QEVADELQKM LLVDELRDAV LLLFANKQDL PNAMAISEMT DKLGLQSLRN
160 170 180
RTWYVQATCA TQGTGLYEGL DWLSNELSKR
Length:180
Mass (Da):20,511
Last modified:January 23, 2007 - v3
Checksum:i3A43EB1F37A81BD9
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti68 – 681V → A.
Corresponds to variant rs11550597 [ dbSNP | Ensembl ].
VAR_048317

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M36341 mRNA. Translation: AAA53081.1.
AF104238
, AF104233, AF104234, AF104235, AF104236, AF104237 Genomic DNA. Translation: AAD54674.1.
AF493883 mRNA. Translation: AAM12597.1.
AK313008 mRNA. Translation: BAG35844.1.
BC003364 mRNA. Translation: AAH03364.1.
BC008753 mRNA. Translation: AAH08753.1.
BC016325 mRNA. Translation: AAH16325.1.
BC022866 mRNA. Translation: AAH22866.1.
CCDSiCCDS2884.1.
PIRiB38622.
RefSeqiNP_001651.1. NM_001660.3.
UniGeneiHs.652183.

Genome annotation databases

EnsembliENST00000303436; ENSP00000306010; ENSG00000168374.
GeneIDi378.
KEGGihsa:378.
UCSCiuc003dix.4. human.

Polymorphism databases

DMDMi114123.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M36341 mRNA. Translation: AAA53081.1 .
AF104238
, AF104233 , AF104234 , AF104235 , AF104236 , AF104237 Genomic DNA. Translation: AAD54674.1 .
AF493883 mRNA. Translation: AAM12597.1 .
AK313008 mRNA. Translation: BAG35844.1 .
BC003364 mRNA. Translation: AAH03364.1 .
BC008753 mRNA. Translation: AAH08753.1 .
BC016325 mRNA. Translation: AAH16325.1 .
BC022866 mRNA. Translation: AAH22866.1 .
CCDSi CCDS2884.1.
PIRi B38622.
RefSeqi NP_001651.1. NM_001660.3.
UniGenei Hs.652183.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1Z6X X-ray 2.70 A/B 1-180 [» ]
ProteinModelPortali P18085.
SMRi P18085. Positions 4-178.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 106873. 22 interactions.
IntActi P18085. 7 interactions.
MINTi MINT-2801449.
STRINGi 9606.ENSP00000306010.

PTM databases

PhosphoSitei P18085.

Polymorphism databases

DMDMi 114123.

Proteomic databases

MaxQBi P18085.
PaxDbi P18085.
PRIDEi P18085.

Protocols and materials databases

DNASUi 378.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000303436 ; ENSP00000306010 ; ENSG00000168374 .
GeneIDi 378.
KEGGi hsa:378.
UCSCi uc003dix.4. human.

Organism-specific databases

CTDi 378.
GeneCardsi GC03M057559.
HGNCi HGNC:655. ARF4.
MIMi 601177. gene.
neXtProti NX_P18085.
PharmGKBi PA24937.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG1100.
GeneTreei ENSGT00760000118835.
HOGENOMi HOG000163691.
HOVERGENi HBG002073.
InParanoidi P18085.
KOi K07939.
OMAi GADELQK.
OrthoDBi EOG77WWDV.
PhylomeDBi P18085.
TreeFami TF300808.

Miscellaneous databases

ChiTaRSi ARF4. human.
EvolutionaryTracei P18085.
GeneWikii ARF4.
GenomeRNAii 378.
NextBioi 1583.
PROi P18085.
SOURCEi Search...

Gene expression databases

Bgeei P18085.
CleanExi HS_ARF4.
ExpressionAtlasi P18085. baseline and differential.
Genevestigatori P18085.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view ]
Pfami PF00025. Arf. 1 hit.
[Graphical view ]
PRINTSi PR00328. SAR1GTPBP.
SMARTi SM00177. ARF. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00231. small_GTP. 1 hit.
PROSITEi PS51417. ARF. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Selective amplification of an mRNA and related pseudogene for a human ADP-ribosylation factor, a guanine nucleotide-dependent protein activator of cholera toxin."
    Monaco L., Murtagh J.J. Jr., Newman K.B., Tsai S.-C., Moss J., Vaughan M.
    Proc. Natl. Acad. Sci. U.S.A. 87:2206-2210(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Human ADP-ribosylation factors. A functionally conserved family of GTP-binding proteins."
    Kahn R.A., Kern F.G., Clark J., Gelmann E.P., Rulka C.
    J. Biol. Chem. 266:2606-2614(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Cloning and characterization of the human ADP-ribosylation factor 4 gene."
    Lebeda R.A., Haun R.S.
    Gene 237:209-214(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
    Puhl H.L. III, Ikeda S.R., Aronstam R.S.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Cerebellum.
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Bone marrow, Placenta, Skin and Urinary bladder.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Structure of human ADP-ribosylation factor 4."
    Structural genomics consortium (SGC)
    Submitted (APR-2005) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) IN COMPLEX WITH GDP.

Entry informationi

Entry nameiARF4_HUMAN
AccessioniPrimary (citable) accession number: P18085
Secondary accession number(s): B2R7J7, P21371
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 156 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Was originally thought to be ARF2.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3