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P18085

- ARF4_HUMAN

UniProt

P18085 - ARF4_HUMAN

Protein

ADP-ribosylation factor 4

Gene

ARF4

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 155 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi24 – 318GTP
    Nucleotide bindingi67 – 715GTP
    Nucleotide bindingi126 – 1294GTP

    GO - Molecular functioni

    1. epidermal growth factor receptor binding Source: UniProt
    2. GTP binding Source: UniProt
    3. protein binding Source: IntAct

    GO - Biological processi

    1. activation of phospholipase D activity Source: UniProt
    2. brain development Source: Ensembl
    3. cell migration Source: UniProt
    4. epidermal growth factor receptor signaling pathway Source: Ensembl
    5. negative regulation of apoptotic process Source: UniProt
    6. positive regulation of transcription from RNA polymerase II promoter Source: UniProt
    7. protein ADP-ribosylation Source: UniProt
    8. protein transport Source: UniProtKB-KW
    9. regulation of reactive oxygen species metabolic process Source: UniProt
    10. response to axon injury Source: Ensembl
    11. small GTPase mediated signal transduction Source: InterPro
    12. vesicle-mediated transport Source: UniProtKB-KW

    Keywords - Biological processi

    ER-Golgi transport, Protein transport, Transport

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ADP-ribosylation factor 4
    Gene namesi
    Name:ARF4
    Synonyms:ARF2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 3

    Organism-specific databases

    HGNCiHGNC:655. ARF4.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: UniProt
    2. extracellular vesicular exosome Source: UniProt
    3. Golgi apparatus Source: UniProtKB-SubCell
    4. membrane Source: UniProtKB
    5. ruffle membrane Source: UniProt

    Keywords - Cellular componenti

    Golgi apparatus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA24937.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedSequence Analysis
    Chaini2 – 180179ADP-ribosylation factor 4PRO_0000207391Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycineSequence Analysis

    Keywords - PTMi

    Lipoprotein, Myristate

    Proteomic databases

    MaxQBiP18085.
    PaxDbiP18085.
    PRIDEiP18085.

    PTM databases

    PhosphoSiteiP18085.

    Expressioni

    Gene expression databases

    ArrayExpressiP18085.
    BgeeiP18085.
    CleanExiHS_ARF4.
    GenevestigatoriP18085.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    RCHY1Q96PM53EBI-1237085,EBI-947779

    Protein-protein interaction databases

    BioGridi106873. 21 interactions.
    IntActiP18085. 7 interactions.
    MINTiMINT-2801449.
    STRINGi9606.ENSP00000306010.

    Structurei

    Secondary structure

    1
    180
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi5 – 84
    Turni9 – 124
    Beta strandi14 – 163
    Beta strandi18 – 258
    Helixi30 – 378
    Beta strandi42 – 487
    Beta strandi51 – 577
    Beta strandi59 – 679
    Beta strandi69 – 713
    Helixi77 – 804
    Beta strandi83 – 9513
    Helixi97 – 993
    Helixi100 – 11213
    Helixi114 – 1163
    Beta strandi120 – 1267
    Helixi136 – 1438
    Helixi145 – 1473
    Beta strandi153 – 1575
    Helixi160 – 1623
    Helixi166 – 17712

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1Z6XX-ray2.70A/B1-180[»]
    ProteinModelPortaliP18085.
    SMRiP18085. Positions 4-178.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP18085.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Arf family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    HOGENOMiHOG000163691.
    HOVERGENiHBG002073.
    InParanoidiP18085.
    KOiK07939.
    OMAiGADELQK.
    OrthoDBiEOG77WWDV.
    PhylomeDBiP18085.
    TreeFamiTF300808.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR024156. Small_GTPase_ARF.
    IPR006689. Small_GTPase_ARF/SAR.
    [Graphical view]
    PfamiPF00025. Arf. 1 hit.
    [Graphical view]
    PRINTSiPR00328. SAR1GTPBP.
    SMARTiSM00177. ARF. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51417. ARF. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P18085-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGLTISSLFS RLFGKKQMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG    50
    FNVETVEYKN ICFTVWDVGG QDRIRPLWKH YFQNTQGLIF VVDSNDRERI 100
    QEVADELQKM LLVDELRDAV LLLFANKQDL PNAMAISEMT DKLGLQSLRN 150
    RTWYVQATCA TQGTGLYEGL DWLSNELSKR 180
    Length:180
    Mass (Da):20,511
    Last modified:January 23, 2007 - v3
    Checksum:i3A43EB1F37A81BD9
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti68 – 681V → A.
    Corresponds to variant rs11550597 [ dbSNP | Ensembl ].
    VAR_048317

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M36341 mRNA. Translation: AAA53081.1.
    AF104238
    , AF104233, AF104234, AF104235, AF104236, AF104237 Genomic DNA. Translation: AAD54674.1.
    AF493883 mRNA. Translation: AAM12597.1.
    AK313008 mRNA. Translation: BAG35844.1.
    BC003364 mRNA. Translation: AAH03364.1.
    BC008753 mRNA. Translation: AAH08753.1.
    BC016325 mRNA. Translation: AAH16325.1.
    BC022866 mRNA. Translation: AAH22866.1.
    CCDSiCCDS2884.1.
    PIRiB38622.
    RefSeqiNP_001651.1. NM_001660.3.
    UniGeneiHs.652183.

    Genome annotation databases

    EnsembliENST00000303436; ENSP00000306010; ENSG00000168374.
    GeneIDi378.
    KEGGihsa:378.
    UCSCiuc003dix.4. human.

    Polymorphism databases

    DMDMi114123.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M36341 mRNA. Translation: AAA53081.1 .
    AF104238
    , AF104233 , AF104234 , AF104235 , AF104236 , AF104237 Genomic DNA. Translation: AAD54674.1 .
    AF493883 mRNA. Translation: AAM12597.1 .
    AK313008 mRNA. Translation: BAG35844.1 .
    BC003364 mRNA. Translation: AAH03364.1 .
    BC008753 mRNA. Translation: AAH08753.1 .
    BC016325 mRNA. Translation: AAH16325.1 .
    BC022866 mRNA. Translation: AAH22866.1 .
    CCDSi CCDS2884.1.
    PIRi B38622.
    RefSeqi NP_001651.1. NM_001660.3.
    UniGenei Hs.652183.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1Z6X X-ray 2.70 A/B 1-180 [» ]
    ProteinModelPortali P18085.
    SMRi P18085. Positions 4-178.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 106873. 21 interactions.
    IntActi P18085. 7 interactions.
    MINTi MINT-2801449.
    STRINGi 9606.ENSP00000306010.

    PTM databases

    PhosphoSitei P18085.

    Polymorphism databases

    DMDMi 114123.

    Proteomic databases

    MaxQBi P18085.
    PaxDbi P18085.
    PRIDEi P18085.

    Protocols and materials databases

    DNASUi 378.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000303436 ; ENSP00000306010 ; ENSG00000168374 .
    GeneIDi 378.
    KEGGi hsa:378.
    UCSCi uc003dix.4. human.

    Organism-specific databases

    CTDi 378.
    GeneCardsi GC03M057532.
    HGNCi HGNC:655. ARF4.
    MIMi 601177. gene.
    neXtProti NX_P18085.
    PharmGKBi PA24937.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1100.
    HOGENOMi HOG000163691.
    HOVERGENi HBG002073.
    InParanoidi P18085.
    KOi K07939.
    OMAi GADELQK.
    OrthoDBi EOG77WWDV.
    PhylomeDBi P18085.
    TreeFami TF300808.

    Miscellaneous databases

    ChiTaRSi ARF4. human.
    EvolutionaryTracei P18085.
    GeneWikii ARF4.
    GenomeRNAii 378.
    NextBioi 1583.
    PROi P18085.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P18085.
    Bgeei P18085.
    CleanExi HS_ARF4.
    Genevestigatori P18085.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR024156. Small_GTPase_ARF.
    IPR006689. Small_GTPase_ARF/SAR.
    [Graphical view ]
    Pfami PF00025. Arf. 1 hit.
    [Graphical view ]
    PRINTSi PR00328. SAR1GTPBP.
    SMARTi SM00177. ARF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51417. ARF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Selective amplification of an mRNA and related pseudogene for a human ADP-ribosylation factor, a guanine nucleotide-dependent protein activator of cholera toxin."
      Monaco L., Murtagh J.J. Jr., Newman K.B., Tsai S.-C., Moss J., Vaughan M.
      Proc. Natl. Acad. Sci. U.S.A. 87:2206-2210(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Human ADP-ribosylation factors. A functionally conserved family of GTP-binding proteins."
      Kahn R.A., Kern F.G., Clark J., Gelmann E.P., Rulka C.
      J. Biol. Chem. 266:2606-2614(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Cloning and characterization of the human ADP-ribosylation factor 4 gene."
      Lebeda R.A., Haun R.S.
      Gene 237:209-214(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
      Puhl H.L. III, Ikeda S.R., Aronstam R.S.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Cerebellum.
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Bone marrow, Placenta, Skin and Urinary bladder.
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Structure of human ADP-ribosylation factor 4."
      Structural genomics consortium (SGC)
      Submitted (APR-2005) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) IN COMPLEX WITH GDP.

    Entry informationi

    Entry nameiARF4_HUMAN
    AccessioniPrimary (citable) accession number: P18085
    Secondary accession number(s): B2R7J7, P21371
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 155 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Caution

    Was originally thought to be ARF2.Curated

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 3
      Human chromosome 3: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3