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Protein

60S ribosomal protein L35a

Gene

RPL35A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the proliferation and viability of hematopoietic cells. Plays a role in 60S ribosomal subunit formation. The protein was found to bind to both initiator and elongator tRNAs and consequently was assigned to the P site or P and A site.1 Publication

GO - Molecular functioni

  1. poly(A) RNA binding Source: UniProtKB
  2. structural constituent of ribosome Source: UniProtKB
  3. tRNA binding Source: UniProtKB-KW

GO - Biological processi

  1. cellular protein metabolic process Source: Reactome
  2. gene expression Source: Reactome
  3. mRNA metabolic process Source: Reactome
  4. nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: Reactome
  5. ribosomal large subunit biogenesis Source: UniProtKB
  6. RNA metabolic process Source: Reactome
  7. rRNA processing Source: UniProtKB
  8. SRP-dependent cotranslational protein targeting to membrane Source: Reactome
  9. translation Source: UniProtKB
  10. translational elongation Source: Reactome
  11. translational initiation Source: Reactome
  12. translational termination Source: Reactome
  13. viral life cycle Source: Reactome
  14. viral process Source: Reactome
  15. viral transcription Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Ligandi

RNA-binding, tRNA-binding

Enzyme and pathway databases

ReactomeiREACT_115902. SRP-dependent cotranslational protein targeting to membrane.
REACT_1404. Peptide chain elongation.
REACT_1797. Formation of a pool of free 40S subunits.
REACT_1986. Eukaryotic Translation Termination.
REACT_2085. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_75768. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_75822. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_79. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_9491. Viral mRNA Translation.

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L35a
Alternative name(s):
Cell growth-inhibiting gene 33 protein
Gene namesi
Name:RPL35A
ORF Names:GIG33
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 3

Organism-specific databases

HGNCiHGNC:10345. RPL35A.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: Reactome
  2. cytosolic large ribosomal subunit Source: UniProtKB
  3. extracellular vesicular exosome Source: UniProtKB
  4. membrane Source: UniProtKB
  5. mitochondrion Source: Ensembl
Complete GO annotation...

Pathology & Biotechi

Involvement in diseasei

Diamond-Blackfan anemia 51 Publication

The disease is caused by mutations affecting the gene represented in this entry.

Disease descriptionA form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies.

See also OMIM:612528
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti27 – 271Missing in DBA5. 1 Publication
VAR_055446
Natural varianti33 – 331V → I in DBA5; may result in aberrant splicing. 1 Publication
VAR_055447

Keywords - Diseasei

Diamond-Blackfan anemia, Disease mutation

Organism-specific databases

MIMi612528. phenotype.
Orphaneti124. Blackfan-Diamond anemia.
PharmGKBiPA34728.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11011060S ribosomal protein L35aPRO_0000192796Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei8 – 81N6-acetyllysine1 Publication
Modified residuei63 – 631N6-acetyllysine; alternateBy similarity
Modified residuei63 – 631N6-succinyllysine; alternateBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP18077.
PaxDbiP18077.
PRIDEiP18077.

2D gel databases

SWISS-2DPAGEP18077.

PTM databases

PhosphoSiteiP18077.

Expressioni

Gene expression databases

BgeeiP18077.
CleanExiHS_RPL35A.
ExpressionAtlasiP18077. baseline and differential.
GenevestigatoriP18077.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CNBPP626331EBI-353383,EBI-1047529
PSTPIP1O435861EBI-353383,EBI-1050964

Protein-protein interaction databases

BioGridi112084. 18 interactions.
IntActiP18077. 10 interactions.
MINTiMINT-1163509.
STRINGi9606.ENSP00000393393.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4V6Xelectron microscopy5.00f1-110[»]
ProteinModelPortaliP18077.
SMRiP18077. Positions 2-110.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L35Ae family.Curated

Phylogenomic databases

eggNOGiCOG2451.
GeneTreeiENSGT00390000016972.
HOGENOMiHOG000195636.
HOVERGENiHBG054581.
InParanoidiP18077.
KOiK02917.
OMAiSTRLYSK.
OrthoDBiEOG741Z4R.
PhylomeDBiP18077.
TreeFamiTF300104.

Family and domain databases

InterProiIPR001780. Ribosomal_L35A.
IPR018266. Ribosomal_L35Ae_CS.
IPR009000. Transl_B-barrel.
[Graphical view]
PANTHERiPTHR10902. PTHR10902. 1 hit.
PfamiPF01247. Ribosomal_L35Ae. 1 hit.
[Graphical view]
ProDomiPD012670. Ribosomal_L35Ae. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF50447. SSF50447. 1 hit.
PROSITEiPS01105. RIBOSOMAL_L35AE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18077-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSGRLWSKAI FAGYKRGLRN QREHTALLKI EGVYARDETE FYLGKRCAYV
60 70 80 90 100
YKAKNNTVTP GGKPNKTRVI WGKVTRAHGN SGMVRAKFRS NLPAKAIGHR
110
IRVMLYPSRI
Length:110
Mass (Da):12,538
Last modified:July 26, 2002 - v2
Checksum:iF32E4A26A25E79E8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti85 – 851R → L in CAA37138. (PubMed:2388839)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti27 – 271Missing in DBA5. 1 Publication
VAR_055446
Natural varianti33 – 331V → I in DBA5; may result in aberrant splicing. 1 Publication
VAR_055447

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52966 mRNA. Translation: CAA37138.1.
AY871273 mRNA. Translation: AAX11429.1.
CH471252 Genomic DNA. Translation: EAW92249.1.
BC001037 mRNA. Translation: AAH01037.1.
BC010949 mRNA. Translation: AAH10949.1.
BC017093 mRNA. Translation: AAH17093.1.
BC061890 mRNA. Translation: AAH61890.1.
X94619 Genomic DNA. Translation: CAA64325.1.
CCDSiCCDS33930.1.
PIRiS12710. R5HU35.
RefSeqiNP_000987.2. NM_000996.2.
XP_005269408.1. XM_005269351.1.
UniGeneiHs.529631.

Genome annotation databases

EnsembliENST00000448864; ENSP00000393393; ENSG00000182899.
ENST00000464167; ENSP00000419117; ENSG00000182899.
GeneIDi6165.
KEGGihsa:6165.
UCSCiuc003fyr.3. human.

Cross-referencesi

Web resourcesi

Diamond-Blackfan Anemia mutation database

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52966 mRNA. Translation: CAA37138.1.
AY871273 mRNA. Translation: AAX11429.1.
CH471252 Genomic DNA. Translation: EAW92249.1.
BC001037 mRNA. Translation: AAH01037.1.
BC010949 mRNA. Translation: AAH10949.1.
BC017093 mRNA. Translation: AAH17093.1.
BC061890 mRNA. Translation: AAH61890.1.
X94619 Genomic DNA. Translation: CAA64325.1.
CCDSiCCDS33930.1.
PIRiS12710. R5HU35.
RefSeqiNP_000987.2. NM_000996.2.
XP_005269408.1. XM_005269351.1.
UniGeneiHs.529631.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4V6Xelectron microscopy5.00f1-110[»]
ProteinModelPortaliP18077.
SMRiP18077. Positions 2-110.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112084. 18 interactions.
IntActiP18077. 10 interactions.
MINTiMINT-1163509.
STRINGi9606.ENSP00000393393.

PTM databases

PhosphoSiteiP18077.

2D gel databases

SWISS-2DPAGEP18077.

Proteomic databases

MaxQBiP18077.
PaxDbiP18077.
PRIDEiP18077.

Protocols and materials databases

DNASUi6165.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000448864; ENSP00000393393; ENSG00000182899.
ENST00000464167; ENSP00000419117; ENSG00000182899.
GeneIDi6165.
KEGGihsa:6165.
UCSCiuc003fyr.3. human.

Organism-specific databases

CTDi6165.
GeneCardsiGC03P197676.
GeneReviewsiRPL35A.
H-InvDBHIX0005661.
HIX0163429.
HIX0170318.
HIX0170319.
HGNCiHGNC:10345. RPL35A.
MIMi180468. gene.
612528. phenotype.
neXtProtiNX_P18077.
Orphaneti124. Blackfan-Diamond anemia.
PharmGKBiPA34728.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG2451.
GeneTreeiENSGT00390000016972.
HOGENOMiHOG000195636.
HOVERGENiHBG054581.
InParanoidiP18077.
KOiK02917.
OMAiSTRLYSK.
OrthoDBiEOG741Z4R.
PhylomeDBiP18077.
TreeFamiTF300104.

Enzyme and pathway databases

ReactomeiREACT_115902. SRP-dependent cotranslational protein targeting to membrane.
REACT_1404. Peptide chain elongation.
REACT_1797. Formation of a pool of free 40S subunits.
REACT_1986. Eukaryotic Translation Termination.
REACT_2085. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_75768. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_75822. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_79. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_9491. Viral mRNA Translation.

Miscellaneous databases

GeneWikiiRPL35A.
GenomeRNAii6165.
NextBioi23949.
PROiP18077.
SOURCEiSearch...

Gene expression databases

BgeeiP18077.
CleanExiHS_RPL35A.
ExpressionAtlasiP18077. baseline and differential.
GenevestigatoriP18077.

Family and domain databases

InterProiIPR001780. Ribosomal_L35A.
IPR018266. Ribosomal_L35Ae_CS.
IPR009000. Transl_B-barrel.
[Graphical view]
PANTHERiPTHR10902. PTHR10902. 1 hit.
PfamiPF01247. Ribosomal_L35Ae. 1 hit.
[Graphical view]
ProDomiPD012670. Ribosomal_L35Ae. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF50447. SSF50447. 1 hit.
PROSITEiPS01105. RIBOSOMAL_L35AE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA encoding the human homologue of rat ribosomal protein L35a."
    Herzog H., Hfferer L., Schneider R., Schweiger M.
    Nucleic Acids Res. 18:4600-4600(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Identification of a cell growth-inhibiting gene."
    Kim J.W., Kim H.K.
    Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Bone marrow, Colon, Ovary and Placenta.
  5. "The human L35a ribosomal protein (RPL35A) gene is located at chromosome band 3q29-qter."
    Colombo P., Read M., Fried M.
    Genomics 32:148-150(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21.
  6. Cited for: FUNCTION, VARIANTS DBA5 LEU-27 DEL AND ILE-33.
  7. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
    Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
    Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-8, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. Cited for: STRUCTURE BY ELECTRON MICROSCOPY (5.0 ANGSTROMS) OF 80S RIBOSOME.

Entry informationi

Entry nameiRL35A_HUMAN
AccessioniPrimary (citable) accession number: P18077
Secondary accession number(s): Q08ES9, Q9BVN7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: July 26, 2002
Last modified: February 4, 2015
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Knockdown of RPL35A in hematopoietic cell lines results in decreased cell proliferation, increased apoptosis, decreased biogenesis of mature 60S ribosomal subunit, and abnormal processing of large ribosomal subunit rRNA.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Ribosomal proteins
    Ribosomal proteins families and list of entries
  7. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.