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P18077

- RL35A_HUMAN

UniProt

P18077 - RL35A_HUMAN

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Protein

60S ribosomal protein L35a

Gene

RPL35A

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Required for the proliferation and viability of hematopoietic cells. Plays a role in 60S ribosomal subunit formation. The protein was found to bind to both initiator and elongator tRNAs and consequently was assigned to the P site or P and A site.1 Publication

GO - Molecular functioni

  1. poly(A) RNA binding Source: UniProtKB
  2. structural constituent of ribosome Source: UniProtKB
  3. tRNA binding Source: UniProtKB-KW

GO - Biological processi

  1. cellular protein metabolic process Source: Reactome
  2. gene expression Source: Reactome
  3. mRNA metabolic process Source: Reactome
  4. nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: Reactome
  5. ribosomal large subunit biogenesis Source: UniProtKB
  6. RNA metabolic process Source: Reactome
  7. rRNA processing Source: UniProtKB
  8. SRP-dependent cotranslational protein targeting to membrane Source: Reactome
  9. translation Source: UniProtKB
  10. translational elongation Source: Reactome
  11. translational initiation Source: Reactome
  12. translational termination Source: Reactome
  13. viral life cycle Source: Reactome
  14. viral process Source: Reactome
  15. viral transcription Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Ligandi

RNA-binding, tRNA-binding

Enzyme and pathway databases

ReactomeiREACT_115902. SRP-dependent cotranslational protein targeting to membrane.
REACT_1404. Peptide chain elongation.
REACT_1797. Formation of a pool of free 40S subunits.
REACT_1986. Eukaryotic Translation Termination.
REACT_2085. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_75768. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_75822. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_79. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_9491. Viral mRNA Translation.

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L35a
Alternative name(s):
Cell growth-inhibiting gene 33 protein
Gene namesi
Name:RPL35A
ORF Names:GIG33
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 3

Organism-specific databases

HGNCiHGNC:10345. RPL35A.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: Reactome
  2. cytosolic large ribosomal subunit Source: UniProtKB
  3. extracellular vesicular exosome Source: UniProt
  4. membrane Source: UniProtKB
  5. mitochondrion Source: Ensembl
Complete GO annotation...

Pathology & Biotechi

Involvement in diseasei

Diamond-Blackfan anemia 5 (DBA5) [MIM:612528]: A form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies.1 Publication
Note: The disease is caused by mutations affecting the gene represented in this entry.
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti27 – 271Missing in DBA5. 1 Publication
VAR_055446
Natural varianti33 – 331V → I in DBA5; may result in aberrant splicing. 1 Publication
VAR_055447

Keywords - Diseasei

Diamond-Blackfan anemia, Disease mutation

Organism-specific databases

MIMi612528. phenotype.
Orphaneti124. Blackfan-Diamond anemia.
PharmGKBiPA34728.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11011060S ribosomal protein L35aPRO_0000192796Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei8 – 81N6-acetyllysine1 Publication
Modified residuei63 – 631N6-acetyllysine; alternateBy similarity
Modified residuei63 – 631N6-succinyllysine; alternateBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP18077.
PaxDbiP18077.
PRIDEiP18077.

2D gel databases

SWISS-2DPAGEP18077.

PTM databases

PhosphoSiteiP18077.

Expressioni

Gene expression databases

BgeeiP18077.
CleanExiHS_RPL35A.
ExpressionAtlasiP18077. baseline and differential.
GenevestigatoriP18077.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CNBPP626331EBI-353383,EBI-1047529
PSTPIP1O435861EBI-353383,EBI-1050964

Protein-protein interaction databases

BioGridi112084. 18 interactions.
IntActiP18077. 10 interactions.
MINTiMINT-1163509.
STRINGi9606.ENSP00000393393.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J3Belectron microscopy5.00f1-110[»]
ProteinModelPortaliP18077.
SMRiP18077. Positions 2-110.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L35Ae family.Curated

Phylogenomic databases

eggNOGiCOG2451.
GeneTreeiENSGT00390000016972.
HOGENOMiHOG000195636.
HOVERGENiHBG054581.
InParanoidiP18077.
KOiK02917.
OMAiSTRLYSK.
OrthoDBiEOG741Z4R.
PhylomeDBiP18077.
TreeFamiTF300104.

Family and domain databases

InterProiIPR001780. Ribosomal_L35A.
IPR018266. Ribosomal_L35Ae_CS.
IPR009000. Transl_B-barrel.
[Graphical view]
PANTHERiPTHR10902. PTHR10902. 1 hit.
PfamiPF01247. Ribosomal_L35Ae. 1 hit.
[Graphical view]
ProDomiPD012670. Ribosomal_L35Ae. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF50447. SSF50447. 1 hit.
PROSITEiPS01105. RIBOSOMAL_L35AE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18077-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSGRLWSKAI FAGYKRGLRN QREHTALLKI EGVYARDETE FYLGKRCAYV
60 70 80 90 100
YKAKNNTVTP GGKPNKTRVI WGKVTRAHGN SGMVRAKFRS NLPAKAIGHR
110
IRVMLYPSRI
Length:110
Mass (Da):12,538
Last modified:July 26, 2002 - v2
Checksum:iF32E4A26A25E79E8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti85 – 851R → L in CAA37138. (PubMed:2388839)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti27 – 271Missing in DBA5. 1 Publication
VAR_055446
Natural varianti33 – 331V → I in DBA5; may result in aberrant splicing. 1 Publication
VAR_055447

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52966 mRNA. Translation: CAA37138.1.
AY871273 mRNA. Translation: AAX11429.1.
CH471252 Genomic DNA. Translation: EAW92249.1.
BC001037 mRNA. Translation: AAH01037.1.
BC010949 mRNA. Translation: AAH10949.1.
BC017093 mRNA. Translation: AAH17093.1.
BC061890 mRNA. Translation: AAH61890.1.
X94619 Genomic DNA. Translation: CAA64325.1.
CCDSiCCDS33930.1.
PIRiS12710. R5HU35.
RefSeqiNP_000987.2. NM_000996.2.
XP_005269408.1. XM_005269351.1.
UniGeneiHs.529631.

Genome annotation databases

EnsembliENST00000448864; ENSP00000393393; ENSG00000182899.
ENST00000464167; ENSP00000419117; ENSG00000182899.
GeneIDi6165.
KEGGihsa:6165.
UCSCiuc003fyr.3. human.

Cross-referencesi

Web resourcesi

Diamond-Blackfan Anemia mutation database

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52966 mRNA. Translation: CAA37138.1 .
AY871273 mRNA. Translation: AAX11429.1 .
CH471252 Genomic DNA. Translation: EAW92249.1 .
BC001037 mRNA. Translation: AAH01037.1 .
BC010949 mRNA. Translation: AAH10949.1 .
BC017093 mRNA. Translation: AAH17093.1 .
BC061890 mRNA. Translation: AAH61890.1 .
X94619 Genomic DNA. Translation: CAA64325.1 .
CCDSi CCDS33930.1.
PIRi S12710. R5HU35.
RefSeqi NP_000987.2. NM_000996.2.
XP_005269408.1. XM_005269351.1.
UniGenei Hs.529631.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3J3B electron microscopy 5.00 f 1-110 [» ]
ProteinModelPortali P18077.
SMRi P18077. Positions 2-110.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 112084. 18 interactions.
IntActi P18077. 10 interactions.
MINTi MINT-1163509.
STRINGi 9606.ENSP00000393393.

PTM databases

PhosphoSitei P18077.

2D gel databases

SWISS-2DPAGE P18077.

Proteomic databases

MaxQBi P18077.
PaxDbi P18077.
PRIDEi P18077.

Protocols and materials databases

DNASUi 6165.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000448864 ; ENSP00000393393 ; ENSG00000182899 .
ENST00000464167 ; ENSP00000419117 ; ENSG00000182899 .
GeneIDi 6165.
KEGGi hsa:6165.
UCSCi uc003fyr.3. human.

Organism-specific databases

CTDi 6165.
GeneCardsi GC03P197676.
GeneReviewsi RPL35A.
H-InvDB HIX0005661.
HIX0163429.
HIX0170318.
HIX0170319.
HGNCi HGNC:10345. RPL35A.
MIMi 180468. gene.
612528. phenotype.
neXtProti NX_P18077.
Orphaneti 124. Blackfan-Diamond anemia.
PharmGKBi PA34728.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG2451.
GeneTreei ENSGT00390000016972.
HOGENOMi HOG000195636.
HOVERGENi HBG054581.
InParanoidi P18077.
KOi K02917.
OMAi STRLYSK.
OrthoDBi EOG741Z4R.
PhylomeDBi P18077.
TreeFami TF300104.

Enzyme and pathway databases

Reactomei REACT_115902. SRP-dependent cotranslational protein targeting to membrane.
REACT_1404. Peptide chain elongation.
REACT_1797. Formation of a pool of free 40S subunits.
REACT_1986. Eukaryotic Translation Termination.
REACT_2085. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_75768. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_75822. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_79. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_9491. Viral mRNA Translation.

Miscellaneous databases

GeneWikii RPL35A.
GenomeRNAii 6165.
NextBioi 23949.
PROi P18077.
SOURCEi Search...

Gene expression databases

Bgeei P18077.
CleanExi HS_RPL35A.
ExpressionAtlasi P18077. baseline and differential.
Genevestigatori P18077.

Family and domain databases

InterProi IPR001780. Ribosomal_L35A.
IPR018266. Ribosomal_L35Ae_CS.
IPR009000. Transl_B-barrel.
[Graphical view ]
PANTHERi PTHR10902. PTHR10902. 1 hit.
Pfami PF01247. Ribosomal_L35Ae. 1 hit.
[Graphical view ]
ProDomi PD012670. Ribosomal_L35Ae. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SUPFAMi SSF50447. SSF50447. 1 hit.
PROSITEi PS01105. RIBOSOMAL_L35AE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA encoding the human homologue of rat ribosomal protein L35a."
    Herzog H., Hfferer L., Schneider R., Schweiger M.
    Nucleic Acids Res. 18:4600-4600(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Identification of a cell growth-inhibiting gene."
    Kim J.W., Kim H.K.
    Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Bone marrow, Colon, Ovary and Placenta.
  5. "The human L35a ribosomal protein (RPL35A) gene is located at chromosome band 3q29-qter."
    Colombo P., Read M., Fried M.
    Genomics 32:148-150(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21.
  6. Cited for: FUNCTION, VARIANTS DBA5 LEU-27 DEL AND ILE-33.
  7. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
    Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
    Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-8, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. Cited for: STRUCTURE BY ELECTRON MICROSCOPY (5.0 ANGSTROMS) OF 80S RIBOSOME.

Entry informationi

Entry nameiRL35A_HUMAN
AccessioniPrimary (citable) accession number: P18077
Secondary accession number(s): Q08ES9, Q9BVN7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: July 26, 2002
Last modified: October 29, 2014
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Knockdown of RPL35A in hematopoietic cell lines results in decreased cell proliferation, increased apoptosis, decreased biogenesis of mature 60S ribosomal subunit, and abnormal processing of large ribosomal subunit rRNA.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Ribosomal proteins
    Ribosomal proteins families and list of entries
  7. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3