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Protein

60S ribosomal protein L35a

Gene

RPL35A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the proliferation and viability of hematopoietic cells. Plays a role in 60S ribosomal subunit formation. The protein was found to bind to both initiator and elongator tRNAs and consequently was assigned to the P site or P and A site.1 Publication

GO - Molecular functioni

  • poly(A) RNA binding Source: UniProtKB
  • structural constituent of ribosome Source: UniProtKB
  • tRNA binding Source: UniProtKB-KW

GO - Biological processi

  • cytoplasmic translation Source: GO_Central
  • nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: Reactome
  • ribosomal large subunit biogenesis Source: UniProtKB
  • rRNA processing Source: UniProtKB
  • SRP-dependent cotranslational protein targeting to membrane Source: Reactome
  • translation Source: UniProtKB
  • translational initiation Source: Reactome
  • viral transcription Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Ligandi

RNA-binding, tRNA-binding

Enzyme and pathway databases

BioCyciZFISH:G66-33814-MONOMER.
ReactomeiR-HSA-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-HSA-156902. Peptide chain elongation.
R-HSA-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-HSA-192823. Viral mRNA Translation.
R-HSA-2408557. Selenocysteine synthesis.
R-HSA-6791226. Major pathway of rRNA processing in the nucleolus and cytosol.
R-HSA-72689. Formation of a pool of free 40S subunits.
R-HSA-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-HSA-72764. Eukaryotic Translation Termination.
R-HSA-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-HSA-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L35a
Alternative name(s):
Cell growth-inhibiting gene 33 protein
Gene namesi
Name:RPL35A
ORF Names:GIG33
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:10345. RPL35A.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Reactome
  • cytosolic large ribosomal subunit Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • extracellular matrix Source: BHF-UCL
  • membrane Source: UniProtKB
  • mitochondrion Source: Ensembl
Complete GO annotation...

Pathology & Biotechi

Involvement in diseasei

Diamond-Blackfan anemia 5 (DBA5)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies.
See also OMIM:612528
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05544627Missing in DBA5. 1 Publication1
Natural variantiVAR_05544733V → I in DBA5; may result in aberrant splicing. 1 PublicationCorresponds to variant rs116840808dbSNPEnsembl.1

Keywords - Diseasei

Diamond-Blackfan anemia, Disease mutation

Organism-specific databases

DisGeNETi6165.
MalaCardsiRPL35A.
MIMi612528. phenotype.
OpenTargetsiENSG00000182899.
Orphaneti124. Blackfan-Diamond anemia.
PharmGKBiPA34728.

Polymorphism and mutation databases

BioMutaiRPL35A.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001927961 – 11060S ribosomal protein L35aAdd BLAST110

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei8N6-acetyllysineCombined sources1
Modified residuei63N6-acetyllysine; alternateBy similarity1
Modified residuei63N6-succinyllysine; alternateBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP18077.
MaxQBiP18077.
PaxDbiP18077.
PeptideAtlasiP18077.
PRIDEiP18077.
TopDownProteomicsiP18077.

2D gel databases

SWISS-2DPAGEP18077.

PTM databases

iPTMnetiP18077.
PhosphoSitePlusiP18077.
SwissPalmiP18077.

Expressioni

Gene expression databases

BgeeiENSG00000182899.
CleanExiHS_RPL35A.
ExpressionAtlasiP18077. baseline and differential.
GenevisibleiP18077. HS.

Interactioni

Protein-protein interaction databases

BioGridi112084. 89 interactors.
IntActiP18077. 17 interactors.
MINTiMINT-1163509.
STRINGi9606.ENSP00000393393.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4UG0electron microscopy-Lf1-110[»]
4V6Xelectron microscopy5.00Cf1-110[»]
5AJ0electron microscopy3.50Af1-110[»]
ProteinModelPortaliP18077.
SMRiP18077.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L35Ae family.Curated

Phylogenomic databases

eggNOGiKOG0887. Eukaryota.
COG2451. LUCA.
GeneTreeiENSGT00390000016972.
HOGENOMiHOG000195636.
HOVERGENiHBG054581.
InParanoidiP18077.
KOiK02917.
OMAiVYARQET.
OrthoDBiEOG091G112U.
PhylomeDBiP18077.
TreeFamiTF300104.

Family and domain databases

InterProiIPR001780. Ribosomal_L35A.
IPR018266. Ribosomal_L35Ae_CS.
IPR009000. Transl_B-barrel.
[Graphical view]
PfamiPF01247. Ribosomal_L35Ae. 1 hit.
[Graphical view]
ProDomiPD012670. Ribosomal_L35Ae. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF50447. SSF50447. 1 hit.
PROSITEiPS01105. RIBOSOMAL_L35AE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18077-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGRLWSKAI FAGYKRGLRN QREHTALLKI EGVYARDETE FYLGKRCAYV
60 70 80 90 100
YKAKNNTVTP GGKPNKTRVI WGKVTRAHGN SGMVRAKFRS NLPAKAIGHR
110
IRVMLYPSRI
Length:110
Mass (Da):12,538
Last modified:July 26, 2002 - v2
Checksum:iF32E4A26A25E79E8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti85R → L in CAA37138 (PubMed:2388839).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05544627Missing in DBA5. 1 Publication1
Natural variantiVAR_05544733V → I in DBA5; may result in aberrant splicing. 1 PublicationCorresponds to variant rs116840808dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52966 mRNA. Translation: CAA37138.1.
AY871273 mRNA. Translation: AAX11429.1.
CH471252 Genomic DNA. Translation: EAW92249.1.
BC001037 mRNA. Translation: AAH01037.1.
BC010949 mRNA. Translation: AAH10949.1.
BC017093 mRNA. Translation: AAH17093.1.
BC061890 mRNA. Translation: AAH61890.1.
X94619 Genomic DNA. Translation: CAA64325.1.
CCDSiCCDS33930.1.
PIRiS12710. R5HU35.
RefSeqiNP_000987.2. NM_000996.2.
NP_001303240.1. NM_001316311.1.
UniGeneiHs.529631.

Genome annotation databases

EnsembliENST00000448864; ENSP00000393393; ENSG00000182899.
ENST00000464167; ENSP00000419117; ENSG00000182899.
GeneIDi6165.
KEGGihsa:6165.
UCSCiuc003fyr.4. human.

Cross-referencesi

Web resourcesi

Diamond-Blackfan Anemia mutation database

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52966 mRNA. Translation: CAA37138.1.
AY871273 mRNA. Translation: AAX11429.1.
CH471252 Genomic DNA. Translation: EAW92249.1.
BC001037 mRNA. Translation: AAH01037.1.
BC010949 mRNA. Translation: AAH10949.1.
BC017093 mRNA. Translation: AAH17093.1.
BC061890 mRNA. Translation: AAH61890.1.
X94619 Genomic DNA. Translation: CAA64325.1.
CCDSiCCDS33930.1.
PIRiS12710. R5HU35.
RefSeqiNP_000987.2. NM_000996.2.
NP_001303240.1. NM_001316311.1.
UniGeneiHs.529631.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4UG0electron microscopy-Lf1-110[»]
4V6Xelectron microscopy5.00Cf1-110[»]
5AJ0electron microscopy3.50Af1-110[»]
ProteinModelPortaliP18077.
SMRiP18077.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112084. 89 interactors.
IntActiP18077. 17 interactors.
MINTiMINT-1163509.
STRINGi9606.ENSP00000393393.

PTM databases

iPTMnetiP18077.
PhosphoSitePlusiP18077.
SwissPalmiP18077.

Polymorphism and mutation databases

BioMutaiRPL35A.

2D gel databases

SWISS-2DPAGEP18077.

Proteomic databases

EPDiP18077.
MaxQBiP18077.
PaxDbiP18077.
PeptideAtlasiP18077.
PRIDEiP18077.
TopDownProteomicsiP18077.

Protocols and materials databases

DNASUi6165.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000448864; ENSP00000393393; ENSG00000182899.
ENST00000464167; ENSP00000419117; ENSG00000182899.
GeneIDi6165.
KEGGihsa:6165.
UCSCiuc003fyr.4. human.

Organism-specific databases

CTDi6165.
DisGeNETi6165.
GeneCardsiRPL35A.
GeneReviewsiRPL35A.
H-InvDBHIX0005661.
HIX0163429.
HIX0170318.
HIX0170319.
HGNCiHGNC:10345. RPL35A.
MalaCardsiRPL35A.
MIMi180468. gene.
612528. phenotype.
neXtProtiNX_P18077.
OpenTargetsiENSG00000182899.
Orphaneti124. Blackfan-Diamond anemia.
PharmGKBiPA34728.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0887. Eukaryota.
COG2451. LUCA.
GeneTreeiENSGT00390000016972.
HOGENOMiHOG000195636.
HOVERGENiHBG054581.
InParanoidiP18077.
KOiK02917.
OMAiVYARQET.
OrthoDBiEOG091G112U.
PhylomeDBiP18077.
TreeFamiTF300104.

Enzyme and pathway databases

BioCyciZFISH:G66-33814-MONOMER.
ReactomeiR-HSA-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-HSA-156902. Peptide chain elongation.
R-HSA-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-HSA-192823. Viral mRNA Translation.
R-HSA-2408557. Selenocysteine synthesis.
R-HSA-6791226. Major pathway of rRNA processing in the nucleolus and cytosol.
R-HSA-72689. Formation of a pool of free 40S subunits.
R-HSA-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-HSA-72764. Eukaryotic Translation Termination.
R-HSA-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-HSA-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

GeneWikiiRPL35A.
GenomeRNAii6165.
PROiP18077.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000182899.
CleanExiHS_RPL35A.
ExpressionAtlasiP18077. baseline and differential.
GenevisibleiP18077. HS.

Family and domain databases

InterProiIPR001780. Ribosomal_L35A.
IPR018266. Ribosomal_L35Ae_CS.
IPR009000. Transl_B-barrel.
[Graphical view]
PfamiPF01247. Ribosomal_L35Ae. 1 hit.
[Graphical view]
ProDomiPD012670. Ribosomal_L35Ae. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF50447. SSF50447. 1 hit.
PROSITEiPS01105. RIBOSOMAL_L35AE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRL35A_HUMAN
AccessioniPrimary (citable) accession number: P18077
Secondary accession number(s): Q08ES9, Q9BVN7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: July 26, 2002
Last modified: November 30, 2016
This is version 155 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Knockdown of RPL35A in hematopoietic cell lines results in decreased cell proliferation, increased apoptosis, decreased biogenesis of mature 60S ribosomal subunit, and abnormal processing of large ribosomal subunit rRNA.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Ribosomal proteins
    Ribosomal proteins families and list of entries
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.