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P18073

- NCAP_I77AB

UniProt

P18073 - NCAP_I77AB

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Protein

Nucleoprotein

Gene

NP

Organism
Influenza A virus (strain A/USSR/90/1977 H1N1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus (By similarity).By similarity

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
  2. structural molecule activity Source: InterPro

GO - Biological processi

  1. viral entry into host cell Source: UniProtKB-KW
  2. viral penetration into host nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

Host-virus interaction, Viral penetration into host nucleus, Virus entry into host cell

Keywords - Ligandi

RNA-binding, Viral nucleoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoprotein
Alternative name(s):
Nucleocapsid protein
Short name:
Protein N
Gene namesi
Name:NP
OrganismiInfluenza A virus (strain A/USSR/90/1977 H1N1)
Taxonomic identifieri381516 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000007793: Genome

Subcellular locationi

GO - Cellular componenti

  1. helical viral capsid Source: UniProtKB-KW
  2. host cell nucleus Source: UniProtKB-KW
  3. ribonucleoprotein complex Source: UniProtKB-KW
  4. viral nucleocapsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Helical capsid protein, Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 498498NucleoproteinPRO_0000079109Add
BLAST

Post-translational modificationi

Late in virus-infected cells, may be cleaved from a 56-kDa protein to a 53-kDa protein by a cellular caspase. This cleavage might be a marker for the onset of apoptosis in infected cells or have a specific function in virus host interaction (By similarity).By similarity

Interactioni

Subunit structurei

Homomultimerizes to form the nucleocapsid. May bind human exportin-1. Binds to viral genomic RNA. Protein-RNA contacts are mediated by a combination of electrostatic interactions between positively charged residues and the phosphate backbone and planar interactions between aromatic side chains and bases (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP18073.
SMRiP18073. Positions 22-496.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1 – 1818Unconventional nuclear localization signalBy similarityAdd
BLAST
Motifi198 – 21619Bipartite nuclear localization signalBy similarityAdd
BLAST

Sequence similaritiesi

Family and domain databases

InterProiIPR002141. Flu_NP.
[Graphical view]
PfamiPF00506. Flu_NP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18073 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASQGTKRSY EQMETDGERQ NATEIRASVG KMIDGIGRFY IQMCTELKLS
60 70 80 90 100
DYEGRLIQNS LTIERMVLSA FDERRNKYLE EHPSAGKDPK KTGGPIYKRV
110 120 130 140 150
DGKWMRELVL YDKEEIRRIW RQANNGDDAT AGLTHMMIWH SNLNDTTYQR
160 170 180 190 200
TRALVRTGMD PRMCSLMQGS TLPRRSGAAG AAVKGVGTMV LELIRMIKRG
210 220 230 240 250
INDRNFWRGE NGRKTRIAYE RMCNILKGKF QTAAQRAMMD QVRESRNPGN
260 270 280 290 300
AEIEDLIFLA RSALILRGSV AHKSCLPACV YGPAVASGYE FEKEGYSLVG
310 320 330 340 350
IDPFKLLQTS QVYSLIRPNE NPAHKSQLVW MACNSAAFED LRVSSFIRGT
360 370 380 390 400
KVIPRGKLST RGVQIASNEN MDTMGSSTLE LRSRYWAIRT RSGGNTNQQR
410 420 430 440 450
ASAGQISIQP TFSVQRNLPF DKTTIMAAFT GNAEGRTSDM RAEIIKMMES
460 470 480 490
ARPEEVSFQG RGVFELSDER AANPIVPSFD MSNEGSYFFG DNAEEYDN
Length:498
Mass (Da):56,037
Last modified:November 1, 1990 - v1
Checksum:i9CD09A429CE16294
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti290 – 2901E → N in ABD95354. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D00603 Genomic RNA. Translation: BAA00479.1.
CY010375 Genomic RNA. Translation: ABD95354.1.
DQ508898 Genomic RNA. Translation: ABF21291.1.
PIRiD36754. VHIVX3.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D00603 Genomic RNA. Translation: BAA00479.1 .
CY010375 Genomic RNA. Translation: ABD95354.1 .
DQ508898 Genomic RNA. Translation: ABF21291.1 .
PIRi D36754. VHIVX3.

3D structure databases

ProteinModelPortali P18073.
SMRi P18073. Positions 22-496.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR002141. Flu_NP.
[Graphical view ]
Pfami PF00506. Flu_NP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Biological and genetic evolution of the nucleoprotein gene of human influenza A viruses."
    Altmueller A., Fitch W.M., Scholtissek C.
    J. Gen. Virol. 70:2111-2119(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  3. "Complete genome sequencing and analysis of selected Influenza Virus vaccine strains spanning six decades (1933-1999)."
    Mbawuike I.N., Zhang Y., Yamada R.E., Nino D., Bui H.-H., Sette A., Couch R.B.
    Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiNCAP_I77AB
AccessioniPrimary (citable) accession number: P18073
Secondary accession number(s): Q1K9I2, Q1WP05
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: October 29, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3