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Protein

Nucleoprotein

Gene

NP

Organism
Influenza A virus (strain A/Fort Warren/1/1950 H1N1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that nucleoprotein binds directly host exportin-1/XPO1 and plays an active role in RNPs nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmasks nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRibonucleoprotein, RNA-binding, Viral nucleoprotein
Biological processHost-virus interaction, Viral penetration into host nucleus, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
NucleoproteinUniRule annotation
Alternative name(s):
Nucleocapsid proteinUniRule annotation
Short name:
Protein NUniRule annotation
Gene namesi
Name:NPUniRule annotation
OrganismiInfluenza A virus (strain A/Fort Warren/1/1950 H1N1)
Taxonomic identifieri384525 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]
Proteomesi
  • UP000167029 Componenti: Genome

Subcellular locationi

  • Virion UniRule annotation
  • Host nucleus UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Capsid protein, Helical capsid protein, Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000790451 – 498NucleoproteinAdd BLAST498

Post-translational modificationi

Late in virus-infected cells, may be cleaved from a 56-kDa protein to a 53-kDa protein by a cellular caspase. This cleavage might be a marker for the onset of apoptosis in infected cells or have a specific function in virus host interaction.UniRule annotation

Interactioni

Subunit structurei

Homomultimerizes to form the nucleocapsid. May bind host exportin-1/XPO1. Binds to viral genomic RNA. Protein-RNA contacts are mediated by a combination of electrostatic interactions between positively charged residues and the phosphate backbone and planar interactions between aromatic side chains and bases.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP18071.
SMRiP18071.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1 – 18Unconventional nuclear localization signalUniRule annotationAdd BLAST18
Motifi198 – 216Bipartite nuclear localization signalUniRule annotationAdd BLAST19

Sequence similaritiesi

Belongs to the influenza viruses nucleoprotein family.UniRule annotation

Family and domain databases

HAMAPiMF_04070. INFV_NCAP. 1 hit.
InterProiView protein in InterPro
IPR002141. Flu_NP.
PfamiView protein in Pfam
PF00506. Flu_NP. 1 hit.

Sequencei

Sequence statusi: Complete.

P18071-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASQGTKRSY EQMETDGDRQ NATEIRASVG KMIDGIGRFY IQMCTELKLS
60 70 80 90 100
DYEGRLIQNS LTIERMVLSA FDERRNKYLE EHPSAGKDPK KTGGPIYKRV
110 120 130 140 150
DGKWMRELVL YDKEEIRRIW RQANNGDDAT AGLTHMMIWH SNLNDTTYQR
160 170 180 190 200
TRALVRTGMD PRMCSLMQGS TLPRRSGAAG AAVKGVGTMV MELIRMIKRG
210 220 230 240 250
INDRNFWRGE NGRKTRIAYE RMCNILKGKF QTAAQRAMMD QVRESRNPGN
260 270 280 290 300
AEIEDLIFLA RSALILRGSV AHKSCLPACV YGPAVASGYD FEKEGYSLVG
310 320 330 340 350
IDPFKLLQNS QVYSLIRPNE NPAHKSQLVW MACNSAAFED LRVSSFIRGT
360 370 380 390 400
KVIPRGKLST RGVQIASNEN MDTMGSSTLE LRSRYWAIRT RSGGNTNQQR
410 420 430 440 450
ASAGQISIQP TFSVQRNLPF DKTTIMAAFT GNAEGRTSDM RAEIIRMMES
460 470 480 490
ARPEEVSFQG RGVFELSDER AANPIVPSFD MSNEGSYFFG DNAEEYDN
Length:498
Mass (Da):56,068
Last modified:November 1, 1990 - v1
Checksum:i10666466BA603775
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00601 Genomic RNA. Translation: BAA00477.1.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiNCAP_I50A0
AccessioniPrimary (citable) accession number: P18071
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: June 7, 2017
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families