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P18070

- NCAP_I78A6

UniProt

P18070 - NCAP_I78A6

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Protein
Nucleoprotein
Gene
NP
Organism
Influenza A virus (strain A/California/10/1978 H1N1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus By similarity.

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
  2. structural molecule activity Source: InterPro

GO - Biological processi

  1. viral entry into host cell Source: UniProtKB-KW
  2. viral penetration into host nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

Host-virus interaction, Viral penetration into host nucleus, Virus entry into host cell

Keywords - Ligandi

RNA-binding, Viral nucleoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoprotein
Alternative name(s):
Nucleocapsid protein
Short name:
Protein N
Gene namesi
Name:NP
OrganismiInfluenza A virus (strain A/California/10/1978 H1N1)
Taxonomic identifieri425557 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]

Subcellular locationi

Virion Reviewed prediction. Host nucleus

GO - Cellular componenti

  1. helical viral capsid Source: UniProtKB-KW
  2. host cell nucleus Source: UniProtKB-SubCell
  3. ribonucleoprotein complex Source: UniProtKB-KW
  4. viral nucleocapsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Helical capsid protein, Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 498498Nucleoprotein
PRO_0000079028Add
BLAST

Post-translational modificationi

Late in virus-infected cells, may be cleaved from a 56-kDa protein to a 53-kDa protein by a cellular caspase. This cleavage might be a marker for the onset of apoptosis in infected cells or have a specific function in virus host interaction By similarity.

Interactioni

Subunit structurei

Homomultimerizes to form the nucleocapsid. May bind human exportin-1. Binds to viral genomic RNA. Protein-RNA contacts are mediated by a combination of electrostatic interactions between positively charged residues and the phosphate backbone and planar interactions between aromatic side chains and bases By similarity.

Structurei

3D structure databases

ProteinModelPortaliP18070.
SMRiP18070. Positions 22-496.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1 – 1818Unconventional nuclear localization signal By similarity
Add
BLAST
Motifi198 – 21619Bipartite nuclear localization signal By similarity
Add
BLAST

Sequence similaritiesi

Family and domain databases

InterProiIPR002141. Flu_NP.
[Graphical view]
PfamiPF00506. Flu_NP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18070-1 [UniParc]FASTAAdd to Basket

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MASQGTKRSY EQMETDGERQ NATEIRASVG KMIDGIGRFY IQMCTELKLS    50
DYEGRLIQNS LTIERMVLSA FDERRNRYLE EHPSAGKDPK KTGGPIYKRV 100
DGKWMRELIL YDKEEIRRIW RQANNGDDAT RGLTHMMIWH SNLNDTTYQR 150
TRALVRTGMD PRMCSLMQGS TLPRRSGAAG AAVKGVGTMV MELIRMIKRG 200
INDRNFWRGE NGRKTRSAYE RMCNILKGKF QTAAQRAMMD QVRESRNPGN 250
AEIEDLIFSA RSALILRGSV AHKSCLPACV YGPAVASGYD FEKEGYSLVG 300
IDPFKLLQNS QVYSLIRPNE NPAHKSQLVW MACHSAAFED LRLLSFIRGT 350
KVSPRGKLST RGVQIASNEN MDTMESSTLE LRSRYWAIRT RSGGNTNQQR 400
ASAGQISVQP TFSVQRNLPF DKSTVMAAFT GNTEGRTSDM RAEIIRMMED 450
AKPEEVSFRG RGVFELSDEK ATNPIVPSFD MSNEGSYFFG DNAEEYDN 498
Length:498
Mass (Da):56,284
Last modified:November 1, 1990 - v1
Checksum:iFAD26B1023B30894
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D00600 Genomic RNA. Translation: BAA00476.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D00600 Genomic RNA. Translation: BAA00476.1 .

3D structure databases

ProteinModelPortali P18070.
SMRi P18070. Positions 22-496.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR002141. Flu_NP.
[Graphical view ]
Pfami PF00506. Flu_NP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Biological and genetic evolution of the nucleoprotein gene of human influenza A viruses."
    Altmueller A., Fitch W.M., Scholtissek C.
    J. Gen. Virol. 70:2111-2119(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiNCAP_I78A6
AccessioniPrimary (citable) accession number: P18070
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: February 19, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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