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P18069

- NCAP_I77AA

UniProt

P18069 - NCAP_I77AA

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Protein

Nucleoprotein

Gene
NP
Organism
Influenza A virus (strain A/Brazil/11/1978 H1N1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus By similarity.

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
  2. structural molecule activity Source: InterPro

GO - Biological processi

  1. viral entry into host cell Source: UniProtKB-KW
  2. viral penetration into host nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

Host-virus interaction, Viral penetration into host nucleus, Virus entry into host cell

Keywords - Ligandi

RNA-binding, Viral nucleoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoprotein
Alternative name(s):
Nucleocapsid protein
Short name:
Protein N
Gene namesi
Name:NP
OrganismiInfluenza A virus (strain A/Brazil/11/1978 H1N1)
Taxonomic identifieri393560 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000008025: Genome

Subcellular locationi

Virion Reviewed prediction. Host nucleus

GO - Cellular componenti

  1. helical viral capsid Source: UniProtKB-KW
  2. host cell nucleus Source: UniProtKB-SubCell
  3. ribonucleoprotein complex Source: UniProtKB-KW
  4. viral nucleocapsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Helical capsid protein, Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 498498NucleoproteinPRO_0000079026Add
BLAST

Post-translational modificationi

Late in virus-infected cells, may be cleaved from a 56-kDa protein to a 53-kDa protein by a cellular caspase. This cleavage might be a marker for the onset of apoptosis in infected cells or have a specific function in virus host interaction By similarity.

Interactioni

Subunit structurei

Homomultimerizes to form the nucleocapsid. May bind human exportin-1. Binds to viral genomic RNA. Protein-RNA contacts are mediated by a combination of electrostatic interactions between positively charged residues and the phosphate backbone and planar interactions between aromatic side chains and bases By similarity.

Structurei

3D structure databases

ProteinModelPortaliP18069.
SMRiP18069. Positions 22-496.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1 – 1818Unconventional nuclear localization signal By similarityAdd
BLAST
Motifi198 – 21619Bipartite nuclear localization signal By similarityAdd
BLAST

Sequence similaritiesi

Family and domain databases

InterProiIPR002141. Flu_NP.
[Graphical view]
PfamiPF00506. Flu_NP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18069-1 [UniParc]FASTAAdd to Basket

« Hide

MASQGTKRSY EQMETDGERQ NATEIRASVG KMIDGIGRFY IQMCTELKLS    50
DYEGRLIQNS LTIERMVLSA FDERRNKYLE EHPSAGKDPK KTGGPIYKRV 100
DGKWMRELVL YDKEEIRRIW RQANNGDDAT AGLTHMMIWH SNLNDTTYQR 150
TRALVRTGMD PRMCSLMQGS TLPRRSGAAG AAVKGVGTMV LELIRIIKRG 200
INDRNFWRGE NGRKTRIAYE RMCNILKGKF QTAAQRAMMD QVRESRNPGN 250
AEIEDLIFLA RSALILRGSV AHKSCLPACV YGPAVASGYA FEKEGYSLVG 300
IDPFKLLQTS QVYSLIRPNE NPAHKSQLVW MACNSAAFED LRVSSFIRGT 350
KVIPRGKLST RGVQIASNEN MDTMGSSTLE LRSRYWAIRT RSGGNTNQQR 400
ASAGQISIQP TFSVQRNLPF DKTTIMAAFT GNAEGRTSDM RAEIIRMMES 450
ARPEEVSFQG RGVFELSDER AANPIVPSFD MSNEGSYFFG DNAEEYDN 498
Length:498
Mass (Da):55,989
Last modified:November 1, 1990 - v1
Checksum:i4B09757425A7F8DA
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti196 – 1961I → M.
Natural varianti446 – 4461R → K.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D00599 Genomic RNA. Translation: BAA00475.1.
CY020296 Viral cRNA. Translation: ABO38069.1.
PIRiE36754. VHIVX4.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D00599 Genomic RNA. Translation: BAA00475.1 .
CY020296 Viral cRNA. Translation: ABO38069.1 .
PIRi E36754. VHIVX4.

3D structure databases

ProteinModelPortali P18069.
SMRi P18069. Positions 22-496.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR002141. Flu_NP.
[Graphical view ]
Pfami PF00506. Flu_NP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Biological and genetic evolution of the nucleoprotein gene of human influenza A viruses."
    Altmueller A., Fitch W.M., Scholtissek C.
    J. Gen. Virol. 70:2111-2119(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  3. The NIAID Influenza Genome Sequencing Consortium
    Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiNCAP_I77AA
AccessioniPrimary (citable) accession number: P18069
Secondary accession number(s): A4GBY1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: February 19, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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