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P18067 (RAB7A_CANFA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ras-related protein Rab-7a
Gene names
Name:RAB7A
Synonyms:RAB7
OrganismCanis familiaris (Dog) (Canis lupus familiaris) [Reference proteome]
Taxonomic identifier9615 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis

Protein attributes

Sequence length207 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Key regulator in endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades. Plays a central role, not only in endosomal traffic, but also in many other cellular and physiological events, such as growth-factor-mediated cell signaling, nutrient-transportor mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism. Also involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes (or vacuoles) and autophagosomes. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and Mycobacteria. Plays a role in the fusion of phagosomes with lysosomes By similarity. Plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses. Microbial pathogens possess survival strategies governed by RAB7A, sometimes by employing RAB7A function (e.g. Salmonella) and sometimes by excluding RAB7A function (e.g. Mycobacterium). In concert with RAC1, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. Controls the endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA. Regulates the endocytic trafficking of the EGF-EGFR complex by regulating its lysosomal degradation. Ref.3 Ref.4 Ref.5

Subunit structure

Interacts with NTRK1/TRKA, RILP, PSMA7, RNF115 and FYCO1. Interacts with the PIK3C3/VPS34-PIK3R4 complex. The GTP-bound form interacts with OSBPL1A and RAC1 By similarity. Interacts with CLN3. Interacts with CHM, the substrate-binding subunit of the Rab geranylgeranyltransferase complex By similarity. Interacts with C9orf72 By similarity.

Subcellular location

Late endosome. Lysosome By similarity. Cytoplasmic vesiclephagosome By similarity. Cytoplasmic vesiclephagosome membrane; Lipid-anchor; Cytoplasmic side By similarity. Melanosome By similarity. Note: Colocalizes with OSBPL1A at the late endosome. Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts. Recruited to phagosomes containing S.aureus or Mycobacterium By similarity.

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

Ontologies

Keywords
   Biological processProtein transport
Transport
   Cellular componentCytoplasmic vesicle
Endosome
Lysosome
Membrane
   LigandGTP-binding
Nucleotide-binding
   PTMLipoprotein
Methylation
Phosphoprotein
Prenylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processearly endosome to late endosome transport

Inferred from mutant phenotype Ref.3. Source: UniProtKB

endosome to lysosome transport

Inferred from electronic annotation. Source: Ensembl

epidermal growth factor catabolic process

Inferred from mutant phenotype Ref.5. Source: UniProtKB

phagosome acidification

Inferred from sequence or structural similarity. Source: UniProtKB

phagosome-lysosome fusion

Inferred from sequence or structural similarity. Source: UniProtKB

protein targeting to lysosome

Inferred from electronic annotation. Source: Ensembl

small GTPase mediated signal transduction

Inferred from electronic annotation. Source: InterPro

   Cellular_componentGolgi apparatus

Inferred from electronic annotation. Source: Ensembl

late endosome

Inferred from sequence or structural similarity. Source: UniProtKB

lysosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

melanosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

phagocytic vesicle

Inferred from sequence or structural similarity. Source: UniProtKB

phagocytic vesicle membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

GTPase activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 207207Ras-related protein Rab-7a
PRO_0000121120

Regions

Nucleotide binding15 – 228GTP By similarity
Nucleotide binding34 – 407GTP By similarity
Nucleotide binding63 – 675GTP By similarity
Nucleotide binding125 – 1284GTP By similarity
Nucleotide binding156 – 1572GTP By similarity
Motif37 – 459Effector region By similarity

Amino acid modifications

Modified residue721Phosphoserine By similarity
Modified residue2071Cysteine methyl ester By similarity
Lipidation2051S-geranylgeranyl cysteine By similarity
Lipidation2071S-geranylgeranyl cysteine By similarity

Sequences

Sequence LengthMass (Da)Tools
P18067 [UniParc].

Last modified November 1, 1990. Version 1.
Checksum: E3AF33B16A67296D

FASTA20723,520
        10         20         30         40         50         60 
MTSRKKVLLK VIILGDSGVG KTSLMNQYVN KKFSNQYKAT IGADFLTKEV MVDDRLVTMQ 

        70         80         90        100        110        120 
IWDTAGQERF QSLGVAFYRG ADCCVLVFDV TAPNTFKTLD SWRDEFLIQA SPRDPENFPF 

       130        140        150        160        170        180 
VVLGNKIDLE NRQVATKRAQ AWCYSKNNIP YFETSAKEAI NVEQAFQTIA RNALKQETEV 

       190        200 
ELYNEFPEPI KLDKNDRAKT SAESCSC 

« Hide

References

[1]"Localization of low molecular weight GTP binding proteins to exocytic and endocytic compartments."
Chavrier P., Parton R.G., Hauri H.P., Simons K., Zerial M.
Cell 62:317-329(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Molecular cloning of YPT1/SEC4-related cDNAs from an epithelial cell line."
Chavrier P., Vingron M., Sander C., Simons K., Zerial M.
Mol. Cell. Biol. 10:6578-6585(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Cocker spaniel.
Tissue: Kidney.
[3]"Rab 7: an important regulator of late endocytic membrane traffic."
Feng Y., Press B., Wandinger-Ness A.
J. Cell Biol. 131:1435-1452(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"Mutant Rab7 causes the accumulation of cathepsin D and cation-independent mannose 6-phosphate receptor in an early endocytic compartment."
Press B., Feng Y., Hoflack B., Wandinger-Ness A.
J. Cell Biol. 140:1075-1089(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"rab7 activity affects epidermal growth factor:epidermal growth factor receptor degradation by regulating endocytic trafficking from the late endosome."
Ceresa B.P., Bahr S.J.
J. Biol. Chem. 281:1099-1106(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M35522 mRNA. Translation: AAA30890.1.
PIRB30413.
RefSeqNP_001003316.1. NM_001003316.1.
XP_005632072.1. XM_005632015.1.
UniGeneCfa.29293.

3D structure databases

ProteinModelPortalP18067.
SMRP18067. Positions 7-190.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP18067. 2 interactions.
MINTMINT-8387239.
STRING9615.ENSCAFP00000034211.

Chemistry

BindingDBP18067.

Proteomic databases

PaxDbP18067.
PRIDEP18067.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSCAFT00000038482; ENSCAFP00000034211; ENSCAFG00000004157.
GeneID404007.
KEGGcfa:404007.

Organism-specific databases

CTD7879.

Phylogenomic databases

eggNOGCOG1100.
GeneTreeENSGT00700000104345.
HOGENOMHOG000233968.
HOVERGENHBG009351.
InParanoidP18067.
KOK07897.
OMADYPDPIK.
OrthoDBEOG7G4QG8.
TreeFamTF105605.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamPF00071. Ras. 1 hit.
[Graphical view]
PRINTSPR00449. RASTRNSFRMNG.
SMARTSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20817494.

Entry information

Entry nameRAB7A_CANFA
AccessionPrimary (citable) accession number: P18067
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: May 14, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families