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Protein

Ras-related protein Rab-5A

Gene

RAB5A

Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed:8521472). RAB5A is required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan. Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 (By similarity).By similarity1 Publication

Enzyme regulationi

Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi27 – 35GTPBy similarity9
Nucleotide bindingi46 – 52GTPBy similarity7
Nucleotide bindingi75 – 79GTPBy similarity5
Nucleotide bindingi133 – 136GTPBy similarity4
Nucleotide bindingi163 – 165GTPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processEndocytosis, Phagocytosis, Protein transport, Transport
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CFA-1660499. Synthesis of PIPs at the plasma membrane.
R-CFA-8854214. TBC/RABGAPs.
R-CFA-8856828. Clathrin-mediated endocytosis.
R-CFA-8873719. RAB geranylgeranylation.
R-CFA-8876198. RAB GEFs exchange GTP for GDP on RABs.
R-CFA-983231. Factors involved in megakaryocyte development and platelet production.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-5A
Gene namesi
Name:RAB5A
OrganismiCanis lupus familiaris (Dog) (Canis familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
Proteomesi
  • UP000002254 Componenti: Chromosome 23

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001211031 – 215Ras-related protein Rab-5AAdd BLAST215

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi212S-geranylgeranyl cysteineBy similarity1
Lipidationi213S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Lipoprotein, Prenylation

Proteomic databases

PaxDbiP18066.
PRIDEiP18066.

Expressioni

Gene expression databases

BgeeiENSCAFG00000005814.

Interactioni

Subunit structurei

Binds EEA1. Interacts with ALS2CL, SUN2, ZFYVE20 and RUFY1. Interacts with RIN1 and GAPVD1, which regulate its pathway, probably by acting as a GEF. Interacts with SGSM1 and SGSM3. Interacts with PIK3CB (By similarity). Interacts with RABEP1 and RINL (PubMed:8521472, PubMed:21419809). Interacts with OCRL and INPP5F. May be a component of a complex composed of RAB5A, DYN2 and PIK3C3 (By similarity). Does not interact with the BLOC-3 complex (heterodimer of HPS1 and HPS4) (PubMed:20048159). Interacts with CLN5 (By similarity).By similarity3 Publications

Protein-protein interaction databases

IntActiP18066. 1 interactor.
STRINGi9615.ENSCAFP00000008690.

Structurei

3D structure databases

ProteinModelPortaliP18066.
SMRiP18066.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi49 – 57Effector regionSequence analysis9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0092. Eukaryota.
ENOG410YCCP. LUCA.
GeneTreeiENSGT00760000119101.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP18066.
KOiK07887.
OMAiGKDACNC.
OrthoDBiEOG091G0HKN.
TreeFamiTF300199.

Family and domain databases

InterProiView protein in InterPro
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
PfamiView protein in Pfam
PF00071. Ras. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiView protein in PROSITE
PS51419. RAB. 1 hit.

Sequencei

Sequence statusi: Complete.

P18066-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANRGATRPN GPNTGNKICQ FKLVLLGESA VGKSSLVLRF VKGQFHEFQE
60 70 80 90 100
STIGAAFLTQ TVCLDDTTVK FEIWDTAGQE RYHSLAPMYY RGAQAAIVVY
110 120 130 140 150
DITNEESFAR AKNWVKELQR QASPNIVIAL SGNKADLANK RAVDFQEAQS
160 170 180 190 200
YADDNSLLFM ETSAKTSMNV NEIFMAIAKK LPKNEPQNPG ANSARGRGVD
210
LTEPTQPTRS QCCSN
Length:215
Mass (Da):23,659
Last modified:November 1, 1990 - v1
Checksum:iDDDE3FD8B37C2606
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35520 mRNA. Translation: AAA30889.1.
PIRiA30413.
RefSeqiNP_001003317.1. NM_001003317.2.
UniGeneiCfa.40292.

Genome annotation databases

EnsembliENSCAFT00000009363; ENSCAFP00000008690; ENSCAFG00000005814.
GeneIDi404008.
KEGGicfa:404008.

Similar proteinsi

Entry informationi

Entry nameiRAB5A_CANLF
AccessioniPrimary (citable) accession number: P18066
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: August 30, 2017
This is version 161 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families