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Protein

Ras-related protein Rab-5A

Gene

RAB5A

Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed:8521472). RAB5A is required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan. Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 (By similarity).By similarity1 Publication

Enzyme regulationi

Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi27 – 359GTPBy similarity
Nucleotide bindingi46 – 527GTPBy similarity
Nucleotide bindingi75 – 795GTPBy similarity
Nucleotide bindingi133 – 1364GTPBy similarity
Nucleotide bindingi163 – 1653GTPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Endocytosis, Phagocytosis, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CFA-983231. Factors involved in megakaryocyte development and platelet production.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-5A
Gene namesi
Name:RAB5A
OrganismiCanis lupus familiaris (Dog) (Canis familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
Proteomesi
  • UP000002254 Componenti: Chromosome 23

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 215215Ras-related protein Rab-5APRO_0000121103Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi212 – 2121S-geranylgeranyl cysteineBy similarity
Lipidationi213 – 2131S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Prenylation

Proteomic databases

PaxDbiP18066.
PRIDEiP18066.

Interactioni

Subunit structurei

Binds EEA1. Interacts with ALS2CL, SUN2, ZFYVE20 and RUFY1. Interacts with RIN1 and GAPVD1, which regulate its pathway, probably by acting as a GEF. Interacts with SGSM1 and SGSM3. Interacts with PIK3CB (By similarity). Interacts with RABEP1 and RINL (PubMed:8521472, PubMed:21419809). Interacts with OCRL and INPP5F. May be a component of a complex composed of RAB5A, DYN2 and PIK3C3 (By similarity).By similarity2 Publications

Protein-protein interaction databases

IntActiP18066. 1 interaction.
STRINGi9615.ENSCAFP00000008690.

Structurei

3D structure databases

ProteinModelPortaliP18066.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi49 – 579Effector regionSequence analysis

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0092. Eukaryota.
ENOG410YCCP. LUCA.
GeneTreeiENSGT00760000119101.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP18066.
KOiK07887.
OMAiPKSQCCS.
OrthoDBiEOG77DJ7M.
TreeFamiTF300199.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18066-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANRGATRPN GPNTGNKICQ FKLVLLGESA VGKSSLVLRF VKGQFHEFQE
60 70 80 90 100
STIGAAFLTQ TVCLDDTTVK FEIWDTAGQE RYHSLAPMYY RGAQAAIVVY
110 120 130 140 150
DITNEESFAR AKNWVKELQR QASPNIVIAL SGNKADLANK RAVDFQEAQS
160 170 180 190 200
YADDNSLLFM ETSAKTSMNV NEIFMAIAKK LPKNEPQNPG ANSARGRGVD
210
LTEPTQPTRS QCCSN
Length:215
Mass (Da):23,659
Last modified:November 1, 1990 - v1
Checksum:iDDDE3FD8B37C2606
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35520 mRNA. Translation: AAA30889.1.
PIRiA30413.
RefSeqiNP_001003317.1. NM_001003317.2.
UniGeneiCfa.40292.

Genome annotation databases

EnsembliENSCAFT00000009363; ENSCAFP00000008690; ENSCAFG00000005814.
GeneIDi404008.
KEGGicfa:404008.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35520 mRNA. Translation: AAA30889.1.
PIRiA30413.
RefSeqiNP_001003317.1. NM_001003317.2.
UniGeneiCfa.40292.

3D structure databases

ProteinModelPortaliP18066.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP18066. 1 interaction.
STRINGi9615.ENSCAFP00000008690.

Proteomic databases

PaxDbiP18066.
PRIDEiP18066.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSCAFT00000009363; ENSCAFP00000008690; ENSCAFG00000005814.
GeneIDi404008.
KEGGicfa:404008.

Organism-specific databases

CTDi5868.

Phylogenomic databases

eggNOGiKOG0092. Eukaryota.
ENOG410YCCP. LUCA.
GeneTreeiENSGT00760000119101.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP18066.
KOiK07887.
OMAiPKSQCCS.
OrthoDBiEOG77DJ7M.
TreeFamiTF300199.

Enzyme and pathway databases

ReactomeiR-CFA-983231. Factors involved in megakaryocyte development and platelet production.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Localization of low molecular weight GTP binding proteins to exocytic and endocytic compartments."
    Chavrier P., Parton R.G., Hauri H.P., Simons K., Zerial M.
    Cell 62:317-329(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
  2. "Molecular cloning of YPT1/SEC4-related cDNAs from an epithelial cell line."
    Chavrier P., Vingron M., Sander C., Simons K., Zerial M.
    Mol. Cell. Biol. 10:6578-6585(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Cocker spaniel.
    Tissue: Kidney.
  3. "Rabaptin-5 is a direct effector of the small GTPase Rab5 in endocytic membrane fusion."
    Stenmark H., Vitale G., Ulrich O., Zerial M.
    Cell 83:423-432(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH RABEP1.
  4. "Rin-like, a novel regulator of endocytosis, acts as guanine nucleotide exchange factor for Rab5a and Rab22."
    Woller B., Luiskandl S., Popovic M., Prieler B.E., Ikonge G., Mutzl M., Rehmann H., Herbst R.
    Biochim. Biophys. Acta 1813:1198-1210(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RINL, ENZYME REGULATION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiRAB5A_CANLF
AccessioniPrimary (citable) accession number: P18066
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: June 8, 2016
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.