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Protein

Guanine nucleotide-binding protein alpha-1 subunit

Gene

GPA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Exhibits a fast rate of basal nucleotide exchange. Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Together with GCR1, may regulate the cell cycle via a signaling cascade that uses phosphatidylinositol-specific phospholipase C (PI-PLC) as an effector and inositol 1,4,5-trisphosphate(IP3) as a second messenger. Promotes abscisic acid (ABA) responses in guard cells. But, together with GCR1 and GB1, acts as a negative regulator of ABA during seed germination and early seedling development. Involved in the blue light (BL) signaling. Together with GCR1 and ADT3, required for BL-mediated synthesis of phenylpyruvate and subsequently of phenylalanine (Phe), in etiolated seedlings. Modulates root architecture (e.g. lateral root formation). Negatively regulated by RGS1.6 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi52 – 521Magnesium
Metal bindingi193 – 1931Magnesium
Binding sitei355 – 3551GTP; via amide nitrogen1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi45 – 528GTP1 Publication
Nucleotide bindingi187 – 1937GTP1 Publication
Nucleotide bindingi218 – 2225GTP1 Publication
Nucleotide bindingi287 – 2904GTP1 Publication

GO - Molecular functioni

  • channel regulator activity Source: TAIR
  • GTPase activity Source: TAIR
  • GTPase inhibitor activity Source: TAIR
  • GTP binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: UniProtKB
  • blue light signaling pathway Source: UniProtKB
  • cell death Source: TAIR
  • cellular response to abscisic acid stimulus Source: UniProtKB
  • gibberellic acid mediated signaling pathway Source: TAIR
  • G-protein coupled receptor signaling pathway, coupled to S1P second messenger Source: TAIR
  • L-phenylalanine biosynthetic process Source: TAIR
  • negative regulation of abscisic acid-activated signaling pathway Source: UniProtKB
  • positive regulation of abscisic acid-activated signaling pathway Source: TAIR
  • reactive oxygen species metabolic process Source: TAIR
  • regulation of cell proliferation Source: TAIR
  • regulation of stomatal movement Source: TAIR
  • response to glucose Source: TAIR
  • response to low fluence blue light stimulus by blue low-fluence system Source: TAIR
  • seed germination Source: UniProtKB
  • thylakoid membrane organization Source: TAIR
  • tyrosine biosynthetic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Keywords - Biological processi

Abscisic acid signaling pathway

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G26300-MONOMER.
ReactomeiR-ATH-399997. Acetylcholine regulates insulin secretion.
R-ATH-416476. G alpha (q) signalling events.
R-ATH-416482. G alpha (12/13) signalling events.
R-ATH-418555. G alpha (s) signalling events.
R-ATH-434316. Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein alpha-1 subunit1 Publication
Short name:
GP-alpha-11 Publication
Gene namesi
Name:GPA11 Publication
Ordered Locus Names:At2g26300Imported
ORF Names:T1D16.6Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G26300.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: TAIR
  • heterotrimeric G-protein complex Source: UniProtKB
  • plasma membrane Source: UniProtKB
  • plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Hypersensitive to abscisic acid (ABA) and to the lipid metabolite, sphingosine-1-phosphate (S1P), a transducer of the ABA signal, but hyposensitive to gibberellins and brassinosteroids. Hypersensitivity to ABA and glucose (Glc) during and after seed germination. Altered response to blue light (BL). Abnormal roots architecture; more auxin-induced lateral roots.5 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved
Chaini2 – 383382Guanine nucleotide-binding protein alpha-1 subunitPRO_0000203618Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine2 Publications
Lipidationi5 – 51S-palmitoyl cysteine1 Publication

Keywords - PTMi

Lipoprotein, Myristate, Palmitate

Proteomic databases

PaxDbiP18064.
PRIDEiP18064.

PTM databases

iPTMnetiP18064.
SwissPalmiP18064.

Expressioni

Tissue specificityi

More abundant in roots and/or leaves.1 Publication

Inductioni

After imbibition and by abscisic acid (ABA) after germination initiation.1 Publication

Gene expression databases

GenevisibleiP18064. AT.

Interactioni

Subunit structurei

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with RGS1, THF1, the pirin protein PRN1, GTG1 and GTG2. Binds to GCR1. May interact with ADT3 (PubMed:12837948, PubMed:14500984, PubMed:15155892, PubMed:16415218, PubMed:16582010, PubMed:17158913, PubMed:17951432, PubMed:19135895, PubMed:21304159). No interactions with RACK1A, RACK1B or RACK1C (PubMed:25731164). Interacts with PLDALPHA1 (PubMed:14594812, PubMed:23913032).12 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GCR1O047146EBI-443890,EBI-443899
GCR2F4IEM55EBI-443890,EBI-1804974
GTG1Q9XIP72EBI-443890,EBI-2214605
GTG2Q0WQG82EBI-443890,EBI-2214623
PLDALPHA1Q388823EBI-443890,EBI-962294
PRN1Q9LX493EBI-443890,EBI-1606661
RGS1Q8H1F26EBI-443890,EBI-1627025
THF1Q9SKT04EBI-443890,EBI-972220

Protein-protein interaction databases

BioGridi2522. 53 interactions.
DIPiDIP-31793N.
IntActiP18064. 17 interactions.
MINTiMINT-6743099.
STRINGi3702.AT2G26300.1.

Structurei

Secondary structure

1
383
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi37 – 448Combined sources
Helixi51 – 6212Combined sources
Helixi68 – 725Combined sources
Helixi75 – 9925Combined sources
Helixi110 – 12516Combined sources
Helixi133 – 14210Combined sources
Helixi146 – 1527Combined sources
Helixi153 – 1575Combined sources
Helixi164 – 1685Combined sources
Helixi171 – 1755Combined sources
Helixi183 – 1886Combined sources
Beta strandi195 – 2017Combined sources
Beta strandi212 – 2198Combined sources
Helixi225 – 2284Combined sources
Helixi230 – 2323Combined sources
Beta strandi236 – 2449Combined sources
Helixi245 – 2473Combined sources
Helixi260 – 27213Combined sources
Helixi275 – 2773Combined sources
Beta strandi281 – 2877Combined sources
Helixi289 – 2957Combined sources
Turni296 – 2983Combined sources
Helixi301 – 3033Combined sources
Helixi305 – 3073Combined sources
Helixi316 – 33722Combined sources
Turni341 – 3455Combined sources
Beta strandi348 – 3525Combined sources
Helixi358 – 37821Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2XTZX-ray2.34A/B/C37-383[»]
ProteinModelPortaliP18064.
SMRiP18064. Positions 37-380.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The helical domain (68-188) is required for self-activation.

Sequence similaritiesi

Belongs to the G-alpha family.Curated

Phylogenomic databases

eggNOGiKOG0082. Eukaryota.
ENOG410XNVQ. LUCA.
HOGENOMiHOG000038730.
InParanoidiP18064.
KOiK19729.
OMAiSARAXYL.
OrthoDBiEOG09360CLR.
PhylomeDBiP18064.

Family and domain databases

Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
IPR002976. Plant_Gprotein_alpha.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR01242. GPROTEINAPLT.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P18064-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLLCSRSRH HTEDTDENTQ AAEIERRIEQ EAKAEKHIRK LLLLGAGESG
60 70 80 90 100
KSTIFKQIKL LFQTGFDEGE LKSYVPVIHA NVYQTIKLLH DGTKEFAQNE
110 120 130 140 150
TDSAKYMLSS ESIAIGEKLS EIGGRLDYPR LTKDIAEGIE TLWKDPAIQE
160 170 180 190 200
TCARGNELQV PDCTKYLMEN LKRLSDINYI PTKEDVLYAR VRTTGVVEIQ
210 220 230 240 250
FSPVGENKKS GEVYRLFDVG GQRNERRKWI HLFEGVTAVI FCAAISEYDQ
260 270 280 290 300
TLFEDEQKNR MMETKELFDW VLKQPCFEKT SFMLFLNKFD IFEKKVLDVP
310 320 330 340 350
LNVCEWFRDY QPVSSGKQEI EHAYEFVKKK FEELYYQNTA PDRVDRVFKI
360 370 380
YRTTALDQKL VKKTFKLVDE TLRRRNLLEA GLL
Length:383
Mass (Da):44,546
Last modified:November 1, 1990 - v1
Checksum:i46BF8650E067F968
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32887 mRNA. Translation: AAA32805.1.
AC004484 Genomic DNA. Translation: AAC14520.1.
CP002685 Genomic DNA. Translation: AEC07820.1.
AY093966 mRNA. Translation: AAM16227.1.
AF385704 mRNA. Translation: AAK60296.1.
PIRiA35864. RGMUOA.
RefSeqiNP_180198.1. NM_128187.3.
UniGeneiAt.25148.

Genome annotation databases

EnsemblPlantsiAT2G26300.1; AT2G26300.1; AT2G26300.
GeneIDi817170.
GrameneiAT2G26300.1; AT2G26300.1; AT2G26300.
KEGGiath:AT2G26300.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32887 mRNA. Translation: AAA32805.1.
AC004484 Genomic DNA. Translation: AAC14520.1.
CP002685 Genomic DNA. Translation: AEC07820.1.
AY093966 mRNA. Translation: AAM16227.1.
AF385704 mRNA. Translation: AAK60296.1.
PIRiA35864. RGMUOA.
RefSeqiNP_180198.1. NM_128187.3.
UniGeneiAt.25148.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2XTZX-ray2.34A/B/C37-383[»]
ProteinModelPortaliP18064.
SMRiP18064. Positions 37-380.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi2522. 53 interactions.
DIPiDIP-31793N.
IntActiP18064. 17 interactions.
MINTiMINT-6743099.
STRINGi3702.AT2G26300.1.

PTM databases

iPTMnetiP18064.
SwissPalmiP18064.

Proteomic databases

PaxDbiP18064.
PRIDEiP18064.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G26300.1; AT2G26300.1; AT2G26300.
GeneIDi817170.
GrameneiAT2G26300.1; AT2G26300.1; AT2G26300.
KEGGiath:AT2G26300.

Organism-specific databases

TAIRiAT2G26300.

Phylogenomic databases

eggNOGiKOG0082. Eukaryota.
ENOG410XNVQ. LUCA.
HOGENOMiHOG000038730.
InParanoidiP18064.
KOiK19729.
OMAiSARAXYL.
OrthoDBiEOG09360CLR.
PhylomeDBiP18064.

Enzyme and pathway databases

BioCyciARA:AT2G26300-MONOMER.
ReactomeiR-ATH-399997. Acetylcholine regulates insulin secretion.
R-ATH-416476. G alpha (q) signalling events.
R-ATH-416482. G alpha (12/13) signalling events.
R-ATH-418555. G alpha (s) signalling events.
R-ATH-434316. Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion.

Miscellaneous databases

PROiP18064.

Gene expression databases

GenevisibleiP18064. AT.

Family and domain databases

Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
IPR002976. Plant_Gprotein_alpha.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR01242. GPROTEINAPLT.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiGPA1_ARATH
AccessioniPrimary (citable) accession number: P18064
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: September 7, 2016
This is version 154 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.