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P18055

- MT2A_RABIT

UniProt

P18055 - MT2A_RABIT

Protein

Metallothionein-2A

Gene
N/A
Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi5 – 51Divalent metal cation; cluster B
    Metal bindingi7 – 71Divalent metal cation; cluster B
    Metal bindingi14 – 141Divalent metal cation; cluster B
    Metal bindingi16 – 161Divalent metal cation; cluster B
    Metal bindingi20 – 201Divalent metal cation; cluster B
    Metal bindingi22 – 221Divalent metal cation; cluster B
    Metal bindingi25 – 251Divalent metal cation; cluster B
    Metal bindingi27 – 271Divalent metal cation; cluster B
    Metal bindingi30 – 301Divalent metal cation; cluster B
    Metal bindingi34 – 341Divalent metal cation; cluster A
    Metal bindingi35 – 351Divalent metal cation; cluster A
    Metal bindingi37 – 371Divalent metal cation; cluster A
    Metal bindingi38 – 381Divalent metal cation; cluster A
    Metal bindingi42 – 421Divalent metal cation; cluster A
    Metal bindingi45 – 451Divalent metal cation; cluster A
    Metal bindingi49 – 491Divalent metal cation; cluster A
    Metal bindingi51 – 511Divalent metal cation; cluster A
    Metal bindingi58 – 581Divalent metal cation; cluster A
    Metal bindingi60 – 601Divalent metal cation; cluster A
    Metal bindingi61 – 611Divalent metal cation; cluster A

    GO - Molecular functioni

    1. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. cellular response to zinc ion Source: UniProtKB
    2. negative regulation of growth Source: UniProtKB

    Keywords - Ligandi

    Cadmium, Copper, Metal-binding, Metal-thiolate cluster, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Metallothionein-2A
    Short name:
    MT-2A
    Alternative name(s):
    Metallothionein-IIA
    Short name:
    MT-IIA
    OrganismiOryctolagus cuniculus (Rabbit)
    Taxonomic identifieri9986 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
    ProteomesiUP000001811: Chromosome 5

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. nucleus Source: UniProtKB
    3. perinuclear region of cytoplasm Source: UniProtKB

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 6262Metallothionein-2APRO_0000197216Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine

    Keywords - PTMi

    Acetylation

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    STRINGi9986.ENSOCUP00000018875.

    Structurei

    Secondary structure

    1
    62
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi10 – 123
    Beta strandi36 – 383
    Beta strandi46 – 483

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1MRBNMR-A32-62[»]
    2MRBNMR-A1-31[»]
    ProteinModelPortaliP18055.
    SMRiP18055. Positions 1-62.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP18055.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 3030BetaAdd
    BLAST
    Regioni31 – 6232AlphaAdd
    BLAST

    Domaini

    Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG138665.
    GeneTreeiENSGT00730000110883.
    HOGENOMiHOG000236262.
    OMAiCANSCTC.
    OrthoDBiEOG7PZS1X.
    TreeFamiTF336054.

    Family and domain databases

    Gene3Di4.10.10.10. 1 hit.
    InterProiIPR017854. Metalthion_dom.
    IPR023587. Metalthion_dom_vert.
    IPR003019. Metalthion_sfam_euk.
    IPR000006. Metalthion_vert.
    IPR018064. Metalthion_vert_metal_BS.
    [Graphical view]
    PANTHERiPTHR23299. PTHR23299. 1 hit.
    PfamiPF00131. Metallothio. 1 hit.
    [Graphical view]
    PRINTSiPR00860. MTVERTEBRATE.
    SUPFAMiSSF57868. SSF57868. 1 hit.
    PROSITEiPS00203. METALLOTHIONEIN_VRT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P18055-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDPNCSCAAA GDSCTCANSC TCKACKCTSC KKSCCSCCPP GCAKCAQGCI   50
    CKGASDKCSC CA 62
    Length:62
    Mass (Da):6,083
    Last modified:November 1, 1990 - v1
    Checksum:iC3FEA7B701081B3E
    GO

    Sequence databases

    PIRiA37425.
    S54336.
    UniGeneiOcu.3423.

    Genome annotation databases

    EnsembliENSOCUT00000023383; ENSOCUP00000018875; ENSOCUG00000021126.

    Cross-referencesi

    Sequence databases

    PIRi A37425.
    S54336.
    UniGenei Ocu.3423.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1MRB NMR - A 32-62 [» ]
    2MRB NMR - A 1-31 [» ]
    ProteinModelPortali P18055.
    SMRi P18055. Positions 1-62.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9986.ENSOCUP00000018875.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSOCUT00000023383 ; ENSOCUP00000018875 ; ENSOCUG00000021126 .

    Phylogenomic databases

    eggNOGi NOG138665.
    GeneTreei ENSGT00730000110883.
    HOGENOMi HOG000236262.
    OMAi CANSCTC.
    OrthoDBi EOG7PZS1X.
    TreeFami TF336054.

    Miscellaneous databases

    EvolutionaryTracei P18055.

    Family and domain databases

    Gene3Di 4.10.10.10. 1 hit.
    InterProi IPR017854. Metalthion_dom.
    IPR023587. Metalthion_dom_vert.
    IPR003019. Metalthion_sfam_euk.
    IPR000006. Metalthion_vert.
    IPR018064. Metalthion_vert_metal_BS.
    [Graphical view ]
    PANTHERi PTHR23299. PTHR23299. 1 hit.
    Pfami PF00131. Metallothio. 1 hit.
    [Graphical view ]
    PRINTSi PR00860. MTVERTEBRATE.
    SUPFAMi SSF57868. SSF57868. 1 hit.
    PROSITEi PS00203. METALLOTHIONEIN_VRT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: PROTEIN SEQUENCE.
      Strain: New Zealand white.
      Tissue: Kidney and Liver.
    2. "Primary structures of seven metallothioneins from rabbit tissue."
      Hunziker P.E., Kaur P., Wan M., Kaenzig A.
      Biochem. J. 306:265-270(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE.
      Strain: New Zealand white.
      Tissue: Kidney and Liver.
    3. "Sequence-specific 1H-NMR assignments in rabbit-liver metallothionein-2."
      Wagner G., Neuhaus D., Worgotter E., Vasak M., Kaegi J.H.R., Wuethrich K.
      Eur. J. Biochem. 157:275-289(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR, PROTEIN SEQUENCE.
    4. "Nuclear magnetic resonance identification of 'half-turn' and 3(10)-helix secondary structure in rabbit liver metallothionein-2."
      Wagner G., Neuhaus D., Worgotter E., Vasak M., Kaegi J.H.R., Wuethrich K.
      J. Mol. Biol. 187:131-135(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR.
    5. "Three-dimensional structure of rabbit liver [Cd7]metallothionein-2a in aqueous solution determined by nuclear magnetic resonance."
      Arseniev A., Schultze P., Worgotter E., Braun W., Wagner G., Vasak M., Kaegi J.H.R., Wuethrich K.
      J. Mol. Biol. 201:637-657(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR.

    Entry informationi

    Entry nameiMT2A_RABIT
    AccessioniPrimary (citable) accession number: P18055
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: November 1, 1990
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Metallothioneins
      Classification of metallothioneins and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3