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P18052 (PTPRA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 142. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Receptor-type tyrosine-protein phosphatase alpha

Short name=Protein-tyrosine phosphatase alpha
Short name=R-PTP-alpha
EC=3.1.3.48
Alternative name(s):
LCA-related phosphatase
PTPTY-28
Gene names
Name:Ptpra
Synonyms:Lrp, Ptpa
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length829 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Widely expressed. Highest expression in brain and kidney. Ref.1 Ref.2 Ref.7

Sequence similarities

Belongs to the protein-tyrosine phosphatase family. Receptor class 4 subfamily.

Contains 2 tyrosine-protein phosphatase domains.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Chl1P702324EBI-6597520,EBI-7703109
Grb2Q606313EBI-6597520,EBI-1688
PrkcbP684033EBI-6597520,EBI-397072From a different organism.
PtprnQ606733EBI-6597520,EBI-8328895
SRCP005232EBI-6597520,EBI-848039From a different organism.
SRCP129313EBI-6597520,EBI-621482From a different organism.
SrcP054802EBI-6597520,EBI-298680

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P18052-1)

Also known as: Long;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P18052-2)

Also known as: Short;

The sequence of this isoform differs from the canonical sequence as follows:
     268-268: F → Y
     269-303: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Probable
Chain20 – 829810Receptor-type tyrosine-protein phosphatase alpha
PRO_0000025434

Regions

Topological domain20 – 142123Extracellular Potential
Transmembrane143 – 16624Helical; Potential
Topological domain167 – 829663Cytoplasmic Potential
Domain232 – 528297Tyrosine-protein phosphatase 1
Domain560 – 818259Tyrosine-protein phosphatase 2
Region469 – 4757Substrate binding By similarity

Sites

Active site4691Phosphocysteine intermediate By similarity
Active site7591Phosphocysteine intermediate By similarity
Binding site4371Substrate By similarity
Binding site5131Substrate By similarity

Amino acid modifications

Modified residue2021Phosphoserine Ref.14
Modified residue2041Phosphoserine Ref.14
Modified residue8251Phosphotyrosine Ref.12 Ref.13 Ref.14
Glycosylation211N-linked (GlcNAc...) Potential
Glycosylation471N-linked (GlcNAc...) Potential
Glycosylation511N-linked (GlcNAc...) Potential
Glycosylation681N-linked (GlcNAc...) Potential
Glycosylation801N-linked (GlcNAc...) Potential
Glycosylation861N-linked (GlcNAc...) Potential
Glycosylation1041N-linked (GlcNAc...) Potential
Glycosylation1241N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence2681F → Y in isoform 2.
VSP_011880
Alternative sequence269 – 30335Missing in isoform 2.
VSP_005146

Experimental info

Sequence conflict2311L → I in AAA39448. Ref.1
Sequence conflict3261S → G Ref.6
Sequence conflict4111C → S Ref.2

Secondary structure

.................................................................................... 829
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Long) [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: 4724A0F477D304B5

FASTA82993,698
        10         20         30         40         50         60 
MDSWFILVLF GSGLIHVSAN NATTVSPSLG TTRLIKTSTT ELAKEENKTS NSTSSVISLS 

        70         80         90        100        110        120 
VAPTFSPNLT LEPTYVTTVN SSHSDNGTRR AASTESGGTT ISPNGSWLIE NQFTDAITEP 

       130        140        150        160        170        180 
WEGNSSTAAT TPETFPPADE TPIIAVMVAL SSLLVIVFII IVLYMLRFKK YKQAGSHSNS 

       190        200        210        220        230        240 
FRLSNGRTED VEPQSVPLLA RSPSTNRKYP PLPVDKLEEE INRRMADDNK LFREEFNALP 

       250        260        270        280        290        300 
ACPIQATCEA ASKEENKEKN RYVNILPFLS LAVSKDAVKA LNKTTPLLER RFIGKSNSRG 

       310        320        330        340        350        360 
CLSDDHSRVH LTPVEGVPDS DYINASFING YQEKNKFIAA QGPKEETVND FWRMIWEQNT 

       370        380        390        400        410        420 
ATIVMVTNLK ERKECKCAQY WPDQGCWTYG NVRVSVEDVT VLVDYTVRKF CIQQVGDVTN 

       430        440        450        460        470        480 
RKPQRLITQF HFTSWPDFGV PFTPIGMLKF LKKVKACNPQ YAGAIVVHCS AGVGRTGTFV 

       490        500        510        520        530        540 
VIDAMLDMMH SERKVDVYGF VSRIRAQRCQ MVQTDMQYVF IYQALLEHYL YGDTELEVTS 

       550        560        570        580        590        600 
LETHLQKIYN KIPGTSNNGL EEEFKKLTSI KIQNDKMRTG NLPANMKKNR VLQIIPYEFN 

       610        620        630        640        650        660 
RVIIPVKRGE ENTDYVNASF IDGYRQKDSY IASQGPLLHT IEDFWRMIWE WKSCSIVMLT 

       670        680        690        700        710        720 
ELEERGQEKC AQYWPSDGLV SYGDITVELK KEEECESYTV RDLLVTNTRE NKSRQIRQFH 

       730        740        750        760        770        780 
FHGWPEVGIP SDGKGMINII AAVQKQQQQS GNHPITVHCS AGAGRTGTFC ALSTVLERVK 

       790        800        810        820 
AEGILDVFQT VKSLRLQRPH MVQTLEQYEF CYKVVQEYID AFSDYANFK 

« Hide

Isoform 2 (Short) [UniParc].

Checksum: 5A282BF9347D18A8
Show »

FASTA79489,957

References

« Hide 'large scale' references
[1]"Identification of an additional member of the protein-tyrosine-phosphatase family: evidence for alternative splicing in the tyrosine phosphatase domain."
Matthews R.J., Cahir E.D., Thomas M.L.
Proc. Natl. Acad. Sci. U.S.A. 87:4444-4448(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
Strain: C57BL/6 X DBA/2.
[2]"Cloning and expression of a widely expressed receptor tyrosine phosphatase."
Sap J., D'Eustachio P., Givol D., Schlessinger J.
Proc. Natl. Acad. Sci. U.S.A. 87:6112-6116(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY.
Strain: BALB/c.
Tissue: Brain.
[3]"Leukocyte common antigen-related phosphatase (LRP) gene structure: conservation of the genomic organization of transmembrane protein tyrosine phosphatases."
Wong E.C., Mullersman J.E., Thomas M.L.
Genomics 17:33-38(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[5]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"Differential expression of a novel murine non-receptor protein tyrosine phosphatase during differentiation of P19 embryonal carcinoma cells."
den Hertog J., Pals C.E., Jonk L.J., Kruijer W.
Biochem. Biophys. Res. Commun. 184:1241-1249(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 322-356.
[7]"Identification of novel protein tyrosine phosphatases of hematopoietic cells by polymerase chain reaction amplification."
Yi T., Cleveland J.L., Ihle J.N.
Blood 78:2222-2228(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 358-467, TISSUE SPECIFICITY.
Strain: BALB/c.
Tissue: Myeloid leukemia cell.
[8]"A novel receptor-type protein tyrosine phosphatase with a single catalytic domain is specifically expressed in mouse brain."
Hendriks W., Schepens J., Brugman C., Zeeuwen P., Wieringa B.
Biochem. J. 305:499-504(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 358-467.
Strain: BALB/c.
Tissue: Brain.
[9]Lubec G., Sunyer B., Chen W.-Q.
Submitted (JAN-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 566-576, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: OF1.
Tissue: Hippocampus.
[10]"Identification and typing of members of the protein-tyrosine phosphatase gene family expressed in mouse brain."
Schepens J., Zeeuwen P., Wieringa B., Hendriks W.
Mol. Biol. Rep. 16:241-248(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 651-756.
Strain: BALB/c.
Tissue: Brain.
[11]"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-825, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Mast cell.
[13]"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-825, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain.
[14]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-202; SER-204 AND TYR-825, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[15]"Structural basis for inhibition of receptor protein-tyrosine phosphatase-alpha by dimerization."
Bilwes A.M., den Hertog J., Hunter T., Noel J.P.
Nature 382:555-559(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 202-503.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M36033 mRNA. Translation: AAA39448.1.
M36034 Genomic DNA. Translation: AAA39449.2.
L13607 Genomic DNA. No translation available.
AL731707 Genomic DNA. Translation: CAM13265.1.
CH466519 Genomic DNA. Translation: EDL28272.1.
Z23054 mRNA. Translation: CAA80589.1.
Z23055 mRNA. Translation: CAA80590.1.
PIRA47373.
RefSeqNP_033006.2. NM_008980.2.
UniGeneMm.224246.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1P15X-ray2.00A/B577-829[»]
1YFOX-ray2.25A/B202-539[»]
ProteinModelPortalP18052.
SMRP18052. Positions 208-821.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid202491. 1 interaction.
IntActP18052. 12 interactions.
MINTMINT-129698.

PTM databases

PhosphoSiteP18052.

Proteomic databases

PaxDbP18052.
PRIDEP18052.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000077303; ENSMUSP00000076533; ENSMUSG00000027303. [P18052-1]
GeneID19262.
KEGGmmu:19262.
UCSCuc008mjc.2. mouse. [P18052-1]

Organism-specific databases

CTD5786.
MGIMGI:97808. Ptpra.

Phylogenomic databases

eggNOGCOG5599.
GeneTreeENSGT00590000082937.
HOGENOMHOG000231464.
HOVERGENHBG053758.
InParanoidA2AHF2.
KOK18032.
OMAKRGEENT.
TreeFamTF351829.

Gene expression databases

ArrayExpressP18052.
BgeeP18052.
CleanExMM_PTPRA.
GenevestigatorP18052.

Family and domain databases

InterProIPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
IPR016336. Tyr_Pase_rcpt_a/e-type.
IPR027262. Tyr_Pase_rcpt_alpha.
[Graphical view]
PfamPF00102. Y_phosphatase. 2 hits.
[Graphical view]
PIRSFPIRSF500808. PTPR_alpha. 1 hit.
PIRSF002006. PTPR_alpha_epsilon. 1 hit.
PRINTSPR00700. PRTYPHPHTASE.
SMARTSM00194. PTPc. 2 hits.
[Graphical view]
PROSITEPS00383. TYR_PHOSPHATASE_1. 2 hits.
PS50056. TYR_PHOSPHATASE_2. 2 hits.
PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP18052.
NextBio296124.
PROP18052.
SOURCESearch...

Entry information

Entry namePTPRA_MOUSE
AccessionPrimary (citable) accession number: P18052
Secondary accession number(s): A2AHF2, Q61808
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 142 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot