P18052 (PTPRA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Receptor-type tyrosine-protein phosphatase alpha
Short name=Protein-tyrosine phosphatase alpha
Short name=R-PTP-alpha
EC=3.1.3.48

Alternative name(s):
LCA-related phosphatase
PTPTY-28

Gene names

Name:	Ptpra
Synonyms:	Lrp, Ptpa

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	829 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Widely expressed. Highest expression in brain and kidney. Ref.1 Ref.2 Ref.7
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Receptor class 4 subfamily. Contains 2 tyrosine-protein phosphatase domains.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Repeat Signal Transmembrane Transmembrane helix
Molecular function	Hydrolase Protein phosphatase
PTM	Glycoprotein Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	insulin receptor signaling pathway Inferred from mutant phenotype PubMed 14733908. Source: MGI peptidyl-tyrosine dephosphorylation Inferred from electronic annotation. Source: GOC protein phosphorylation Inferred from direct assay PubMed 14733908. Source: MGI
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P18052-1)

Also known as: Long;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P18052-2)

Also known as: Short;

The sequence of this isoform differs from the canonical sequence as follows:
     268-268: F → Y
     269-303: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 19

19

Probable

Chain

20 – 829

810

Receptor-type tyrosine-protein phosphatase alpha

PRO_0000025434

Regions

Topological domain

20 – 142

123

Extracellular Potential

Transmembrane

143 – 166

24

Helical; Potential

Topological domain

167 – 829

663

Cytoplasmic Potential

Domain

232 – 528

297

Tyrosine-protein phosphatase 1

Domain

560 – 818

259

Tyrosine-protein phosphatase 2

Region

469 – 475

7

Substrate binding By similarity

Sites

Active site

469

1

Phosphocysteine intermediate By similarity

Active site

759

1

Phosphocysteine intermediate By similarity

Binding site

437

1

Substrate By similarity

Binding site

513

1

Substrate By similarity

Amino acid modifications

Modified residue

180

1

Phosphoserine Ref.13

Modified residue

204

1

Phosphoserine Ref.13

Modified residue

825

1

Phosphotyrosine Ref.11 Ref.12 Ref.13

Glycosylation

21

1

N-linked (GlcNAc...) Potential

Glycosylation

47

1

N-linked (GlcNAc...) Potential

Glycosylation

51

1

N-linked (GlcNAc...) Potential

Glycosylation

68

1

N-linked (GlcNAc...) Potential

Glycosylation

80

1

N-linked (GlcNAc...) Potential

Glycosylation

86

1

N-linked (GlcNAc...) Potential

Glycosylation

104

1

N-linked (GlcNAc...) Potential

Glycosylation

124

1

N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence

268

1

F → Y in isoform 2.

VSP_011880

Alternative sequence

269 – 303

35

Missing in isoform 2.

VSP_005146

Experimental info

Sequence conflict

231

1

L → I in AAA39448. Ref.1

Sequence conflict

326

1

S → G Ref.6

Sequence conflict

411

1

C → S Ref.2

Secondary structure

1

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829

Helix Strand Turn

Details...

Helix

214 – 238

25

Helix

249 – 252

4

Helix

254 – 259

6

Turn

305 – 307

3

Turn

318 – 321

4

Beta strand

324 – 330

7

Beta strand

333 – 340

8

Helix

345 – 347

3

Helix

348 – 357

10

Beta strand

362 – 365

4

Beta strand

369 – 371

3

Beta strand

383 – 389

7

Beta strand

392 – 401

10

Beta strand

403 – 413

11

Beta strand

425 – 432

8

Beta strand

437 – 439

3

Helix

445 – 457

13

Beta strand

465 – 468

4

Beta strand

470 – 473

4

Helix

474 – 491

18

Beta strand

492 – 495

4

Helix

497 – 504

8

Turn

505 – 507

3

Helix

515 – 530

16

Helix

579 – 581

3

Turn

583 – 585

3

Beta strand

608 – 612

5

Beta strand

617 – 621

5

Beta strand

630 – 633

4

Beta strand

638 – 640

3

Helix

641 – 650

10

Beta strand

655 – 658

4

Beta strand

664 – 667

4

Beta strand

676 – 678

3

Beta strand

696 – 706

11

Beta strand

712 – 721

10

Beta strand

726 – 728

3

Beta strand

731 – 733

3

Helix

736 – 746

11

Turn

747 – 751

5

Beta strand

755 – 763

9

Helix

764 – 782

19

Helix

789 – 795

7

Turn

805 – 808

4

Helix

809 – 817

9

Turn

818 – 820

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Long) [UniParc]. Last modified July 27, 2011. Version 3. Checksum: 4724A0F477D304B5	FASTA	829	93,698
	10 20 30 40 50 60 MDSWFILVLF GSGLIHVSAN NATTVSPSLG TTRLIKTSTT ELAKEENKTS NSTSSVISLS 70 80 90 100 110 120 VAPTFSPNLT LEPTYVTTVN SSHSDNGTRR AASTESGGTT ISPNGSWLIE NQFTDAITEP 130 140 150 160 170 180 WEGNSSTAAT TPETFPPADE TPIIAVMVAL SSLLVIVFII IVLYMLRFKK YKQAGSHSNS 190 200 210 220 230 240 FRLSNGRTED VEPQSVPLLA RSPSTNRKYP PLPVDKLEEE INRRMADDNK LFREEFNALP 250 260 270 280 290 300 ACPIQATCEA ASKEENKEKN RYVNILPFLS LAVSKDAVKA LNKTTPLLER RFIGKSNSRG 310 320 330 340 350 360 CLSDDHSRVH LTPVEGVPDS DYINASFING YQEKNKFIAA QGPKEETVND FWRMIWEQNT 370 380 390 400 410 420 ATIVMVTNLK ERKECKCAQY WPDQGCWTYG NVRVSVEDVT VLVDYTVRKF CIQQVGDVTN 430 440 450 460 470 480 RKPQRLITQF HFTSWPDFGV PFTPIGMLKF LKKVKACNPQ YAGAIVVHCS AGVGRTGTFV 490 500 510 520 530 540 VIDAMLDMMH SERKVDVYGF VSRIRAQRCQ MVQTDMQYVF IYQALLEHYL YGDTELEVTS 550 560 570 580 590 600 LETHLQKIYN KIPGTSNNGL EEEFKKLTSI KIQNDKMRTG NLPANMKKNR VLQIIPYEFN 610 620 630 640 650 660 RVIIPVKRGE ENTDYVNASF IDGYRQKDSY IASQGPLLHT IEDFWRMIWE WKSCSIVMLT 670 680 690 700 710 720 ELEERGQEKC AQYWPSDGLV SYGDITVELK KEEECESYTV RDLLVTNTRE NKSRQIRQFH 730 740 750 760 770 780 FHGWPEVGIP SDGKGMINII AAVQKQQQQS GNHPITVHCS AGAGRTGTFC ALSTVLERVK 790 800 810 820 AEGILDVFQT VKSLRLQRPH MVQTLEQYEF CYKVVQEYID AFSDYANFK
	Isoform 2 (Short) [UniParc]. Checksum: 5A282BF9347D18A8	FASTA	794	89,957
	10 20 30 40 50 60 MDSWFILVLF GSGLIHVSAN NATTVSPSLG TTRLIKTSTT ELAKEENKTS NSTSSVISLS 70 80 90 100 110 120 VAPTFSPNLT LEPTYVTTVN SSHSDNGTRR AASTESGGTT ISPNGSWLIE NQFTDAITEP 130 140 150 160 170 180 WEGNSSTAAT TPETFPPADE TPIIAVMVAL SSLLVIVFII IVLYMLRFKK YKQAGSHSNS 190 200 210 220 230 240 FRLSNGRTED VEPQSVPLLA RSPSTNRKYP PLPVDKLEEE INRRMADDNK LFREEFNALP 250 260 270 280 290 300 ACPIQATCEA ASKEENKEKN RYVNILPYDD HSRVHLTPVE GVPDSDYINA SFINGYQEKN 310 320 330 340 350 360 KFIAAQGPKE ETVNDFWRMI WEQNTATIVM VTNLKERKEC KCAQYWPDQG CWTYGNVRVS 370 380 390 400 410 420 VEDVTVLVDY TVRKFCIQQV GDVTNRKPQR LITQFHFTSW PDFGVPFTPI GMLKFLKKVK 430 440 450 460 470 480 ACNPQYAGAI VVHCSAGVGR TGTFVVIDAM LDMMHSERKV DVYGFVSRIR AQRCQMVQTD 490 500 510 520 530 540 MQYVFIYQAL LEHYLYGDTE LEVTSLETHL QKIYNKIPGT SNNGLEEEFK KLTSIKIQND 550 560 570 580 590 600 KMRTGNLPAN MKKNRVLQII PYEFNRVIIP VKRGEENTDY VNASFIDGYR QKDSYIASQG 610 620 630 640 650 660 PLLHTIEDFW RMIWEWKSCS IVMLTELEER GQEKCAQYWP SDGLVSYGDI TVELKKEEEC 670 680 690 700 710 720 ESYTVRDLLV TNTRENKSRQ IRQFHFHGWP EVGIPSDGKG MINIIAAVQK QQQQSGNHPI 730 740 750 760 770 780 TVHCSAGAGR TGTFCALSTV LERVKAEGIL DVFQTVKSLR LQRPHMVQTL EQYEFCYKVV 790 QEYIDAFSDY ANFK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of an additional member of the protein-tyrosine-phosphatase family: evidence for alternative splicing in the tyrosine phosphatase domain." Matthews R.J., Cahir E.D., Thomas M.L. Proc. Natl. Acad. Sci. U.S.A. 87:4444-4448(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY. Strain: C57BL/6 X DBA/2.
[2]	"Cloning and expression of a widely expressed receptor tyrosine phosphatase." Sap J., D'Eustachio P., Givol D., Schlessinger J. Proc. Natl. Acad. Sci. U.S.A. 87:6112-6116(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY. Strain: BALB/c. Tissue: Brain.
[3]	"Leukocyte common antigen-related phosphatase (LRP) gene structure: conservation of the genomic organization of transmembrane protein tyrosine phosphatases." Wong E.C., Mullersman J.E., Thomas M.L. Genomics 17:33-38(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[5]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"Differential expression of a novel murine non-receptor protein tyrosine phosphatase during differentiation of P19 embryonal carcinoma cells." den Hertog J., Pals C.E., Jonk L.J., Kruijer W. Biochem. Biophys. Res. Commun. 184:1241-1249(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 322-356.
[7]	"Identification of novel protein tyrosine phosphatases of hematopoietic cells by polymerase chain reaction amplification." Yi T., Cleveland J.L., Ihle J.N. Blood 78:2222-2228(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 358-467, TISSUE SPECIFICITY. Strain: BALB/c. Tissue: Myeloid leukemia cell.
[8]	"A novel receptor-type protein tyrosine phosphatase with a single catalytic domain is specifically expressed in mouse brain." Hendriks W., Schepens J., Brugman C., Zeeuwen P., Wieringa B. Biochem. J. 305:499-504(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 358-467. Strain: BALB/c. Tissue: Brain.
[9]	Lubec G., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 566-576, MASS SPECTROMETRY. Strain: OF1. Tissue: Hippocampus.
[10]	"Identification and typing of members of the protein-tyrosine phosphatase gene family expressed in mouse brain." Schepens J., Zeeuwen P., Wieringa B., Hendriks W. Mol. Biol. Rep. 16:241-248(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 651-756. Strain: BALB/c. Tissue: Brain.
[11]	"Quantitative time-resolved phosphoproteomic analysis of mast cell signaling." Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R. J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-825, MASS SPECTROMETRY. Tissue: Mast cell.
[12]	"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P. J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-825, MASS SPECTROMETRY. Tissue: Brain.
[13]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-180; SER-204 AND TYR-825, MASS SPECTROMETRY. Tissue: Macrophage.
[14]	"Structural basis for inhibition of receptor protein-tyrosine phosphatase-alpha by dimerization." Bilwes A.M., den Hertog J., Hunter T., Noel J.P. Nature 382:555-559(1996) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 202-503.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M36033 mRNA. Translation: AAA39448.1.
M36034 Genomic DNA. Translation: AAA39449.2.
L13607 Genomic DNA. No translation available.
AL731707 Genomic DNA. Translation: CAM13265.1.
CH466519 Genomic DNA. Translation: EDL28272.1.
Z23054 mRNA. Translation: CAA80589.1.
Z23055 mRNA. Translation: CAA80590.1.

IPI

IPI00108685.
IPI00336550.

PIR

A47373.

RefSeq

NP_033006.2. NM_008980.2.

UniGene

Mm.224246.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1P15	X-ray	2.00	A/B	577-829	[»]
1YFO	X-ray	2.25	A/B	202-539	[»]

ProteinModelPortal

P18052.

SMR

P18052. Positions 208-821.

ModBase

Search...

Protein-protein interaction databases

MINT

MINT-129698.

PTM databases

PhosphoSite

P18052.

Proteomic databases

PaxDb

P18052.

PRIDE

P18052.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000077303; ENSMUSP00000076533; ENSMUSG00000027303.

GeneID

19262.

KEGG

mmu:19262.

Organism-specific databases

CTD

5786.

MGI

MGI:97808. Ptpra.

Phylogenomic databases

eggNOG

COG5599.

GeneTree

ENSGT00590000082937.

HOGENOM

HOG000231464.

HOVERGEN

HBG053758.

InParanoid

A2AHF2.

KO

K01104.

OMA

KRGEENT.

Gene expression databases

ArrayExpress

P18052.

Bgee

P18052.

CleanEx

MM_PTPRA.

Genevestigator

P18052.

GermOnline

ENSMUSG00000027303. Mus musculus.

Family and domain databases

InterPro

IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
IPR016336. Tyr_Pase_rcpt_a/e-type.
IPR027262. Tyr_Pase_rcpt_alpha.
[Graphical view]

Pfam

PF00102. Y_phosphatase. 2 hits.
[Graphical view]

PIRSF

PIRSF500808. PTPR_alpha. 1 hit.
PIRSF002006. PTPR_alpha_epsilon. 1 hit.

PRINTS

PR00700. PRTYPHPHTASE.

SMART

SM00194. PTPc. 2 hits.
[Graphical view]

PROSITE

PS00383. TYR_PHOSPHATASE_1. 2 hits.
PS50056. TYR_PHOSPHATASE_2. 2 hits.
PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P18052.

NextBio

296124.

SOURCE

Search...

Entry information

Entry name

PTPRA_MOUSE

Accession

Primary (citable) accession number: P18052
Secondary accession number(s): A2AHF2, Q61808

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 133 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program