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P18052

- PTPRA_MOUSE

UniProt

P18052 - PTPRA_MOUSE

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Protein

Receptor-type tyrosine-protein phosphatase alpha

Gene
Ptpra, Lrp, Ptpa
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei437 – 4371Substrate By similarity
Active sitei469 – 4691Phosphocysteine intermediate By similarity
Binding sitei513 – 5131Substrate By similarity
Active sitei759 – 7591Phosphocysteine intermediate By similarity

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. protein tyrosine phosphatase activity Source: UniProtKB-EC

GO - Biological processi

  1. insulin receptor signaling pathway Source: MGI
  2. protein phosphorylation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Names & Taxonomyi

Protein namesi
Recommended name:
Receptor-type tyrosine-protein phosphatase alpha (EC:3.1.3.48)
Short name:
Protein-tyrosine phosphatase alpha
Short name:
R-PTP-alpha
Alternative name(s):
LCA-related phosphatase
PTPTY-28
Gene namesi
Name:Ptpra
Synonyms:Lrp, Ptpa
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:97808. Ptpra.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 142123Extracellular Reviewed predictionAdd
BLAST
Transmembranei143 – 16624Helical; Reviewed predictionAdd
BLAST
Topological domaini167 – 829663Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. receptor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919 InferredAdd
BLAST
Chaini20 – 829810Receptor-type tyrosine-protein phosphatase alphaPRO_0000025434Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi21 – 211N-linked (GlcNAc...) Reviewed prediction
Glycosylationi47 – 471N-linked (GlcNAc...) Reviewed prediction
Glycosylationi51 – 511N-linked (GlcNAc...) Reviewed prediction
Glycosylationi68 – 681N-linked (GlcNAc...) Reviewed prediction
Glycosylationi80 – 801N-linked (GlcNAc...) Reviewed prediction
Glycosylationi86 – 861N-linked (GlcNAc...) Reviewed prediction
Glycosylationi104 – 1041N-linked (GlcNAc...) Reviewed prediction
Glycosylationi124 – 1241N-linked (GlcNAc...) Reviewed prediction
Modified residuei202 – 2021Phosphoserine1 Publication
Modified residuei204 – 2041Phosphoserine1 Publication
Modified residuei825 – 8251Phosphotyrosine3 Publications

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP18052.
PaxDbiP18052.
PRIDEiP18052.

PTM databases

PhosphoSiteiP18052.

Expressioni

Tissue specificityi

Widely expressed. Highest expression in brain and kidney.3 Publications

Gene expression databases

ArrayExpressiP18052.
BgeeiP18052.
CleanExiMM_PTPRA.
GenevestigatoriP18052.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Chl1P702324EBI-6597520,EBI-7703109
Grb2Q606314EBI-6597520,EBI-1688
PrkcbP684033EBI-6597520,EBI-397072From a different organism.
PtprnQ606733EBI-6597520,EBI-8328895
SRCP005232EBI-6597520,EBI-848039From a different organism.
SRCP129313EBI-6597520,EBI-621482From a different organism.
SrcP054802EBI-6597520,EBI-298680

Protein-protein interaction databases

BioGridi202491. 1 interaction.
IntActiP18052. 12 interactions.
MINTiMINT-129698.

Structurei

Secondary structure

1
829
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi214 – 23825
Helixi249 – 2524
Helixi254 – 2596
Turni305 – 3073
Turni318 – 3214
Beta strandi324 – 3307
Beta strandi333 – 3408
Helixi345 – 3473
Helixi348 – 35710
Beta strandi362 – 3654
Beta strandi369 – 3713
Beta strandi383 – 3897
Beta strandi392 – 40110
Beta strandi403 – 41311
Beta strandi425 – 4328
Beta strandi437 – 4393
Helixi445 – 45713
Beta strandi465 – 4684
Beta strandi470 – 4734
Helixi474 – 49118
Beta strandi492 – 4954
Helixi497 – 5048
Turni505 – 5073
Helixi515 – 53016
Helixi579 – 5813
Turni583 – 5853
Beta strandi608 – 6125
Beta strandi617 – 6215
Beta strandi630 – 6334
Beta strandi638 – 6403
Helixi641 – 65010
Beta strandi655 – 6584
Beta strandi664 – 6674
Beta strandi676 – 6783
Beta strandi696 – 70611
Beta strandi712 – 72110
Beta strandi726 – 7283
Beta strandi731 – 7333
Helixi736 – 74611
Turni747 – 7515
Beta strandi755 – 7639
Helixi764 – 78219
Helixi789 – 7957
Turni805 – 8084
Helixi809 – 8179
Turni818 – 8203

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1P15X-ray2.00A/B577-829[»]
1YFOX-ray2.25A/B202-539[»]
ProteinModelPortaliP18052.
SMRiP18052. Positions 208-821.

Miscellaneous databases

EvolutionaryTraceiP18052.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini232 – 528297Tyrosine-protein phosphatase 1Add
BLAST
Domaini560 – 818259Tyrosine-protein phosphatase 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni469 – 4757Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5599.
GeneTreeiENSGT00590000082937.
HOGENOMiHOG000231464.
HOVERGENiHBG053758.
InParanoidiA2AHF2.
KOiK18032.
OMAiKRGEENT.
TreeFamiTF351829.

Family and domain databases

Gene3Di3.90.190.10. 3 hits.
InterProiIPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
IPR016336. Tyr_Pase_rcpt_a/e-type.
IPR027262. Tyr_Pase_rcpt_alpha.
[Graphical view]
PfamiPF00102. Y_phosphatase. 2 hits.
[Graphical view]
PIRSFiPIRSF500808. PTPR_alpha. 1 hit.
PIRSF002006. PTPR_alpha_epsilon. 1 hit.
PRINTSiPR00700. PRTYPHPHTASE.
SMARTiSM00194. PTPc. 2 hits.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 3 hits.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 2 hits.
PS50056. TYR_PHOSPHATASE_2. 2 hits.
PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P18052-1) [UniParc]FASTAAdd to Basket

Also known as: Long

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MDSWFILVLF GSGLIHVSAN NATTVSPSLG TTRLIKTSTT ELAKEENKTS    50
NSTSSVISLS VAPTFSPNLT LEPTYVTTVN SSHSDNGTRR AASTESGGTT 100
ISPNGSWLIE NQFTDAITEP WEGNSSTAAT TPETFPPADE TPIIAVMVAL 150
SSLLVIVFII IVLYMLRFKK YKQAGSHSNS FRLSNGRTED VEPQSVPLLA 200
RSPSTNRKYP PLPVDKLEEE INRRMADDNK LFREEFNALP ACPIQATCEA 250
ASKEENKEKN RYVNILPFLS LAVSKDAVKA LNKTTPLLER RFIGKSNSRG 300
CLSDDHSRVH LTPVEGVPDS DYINASFING YQEKNKFIAA QGPKEETVND 350
FWRMIWEQNT ATIVMVTNLK ERKECKCAQY WPDQGCWTYG NVRVSVEDVT 400
VLVDYTVRKF CIQQVGDVTN RKPQRLITQF HFTSWPDFGV PFTPIGMLKF 450
LKKVKACNPQ YAGAIVVHCS AGVGRTGTFV VIDAMLDMMH SERKVDVYGF 500
VSRIRAQRCQ MVQTDMQYVF IYQALLEHYL YGDTELEVTS LETHLQKIYN 550
KIPGTSNNGL EEEFKKLTSI KIQNDKMRTG NLPANMKKNR VLQIIPYEFN 600
RVIIPVKRGE ENTDYVNASF IDGYRQKDSY IASQGPLLHT IEDFWRMIWE 650
WKSCSIVMLT ELEERGQEKC AQYWPSDGLV SYGDITVELK KEEECESYTV 700
RDLLVTNTRE NKSRQIRQFH FHGWPEVGIP SDGKGMINII AAVQKQQQQS 750
GNHPITVHCS AGAGRTGTFC ALSTVLERVK AEGILDVFQT VKSLRLQRPH 800
MVQTLEQYEF CYKVVQEYID AFSDYANFK 829
Length:829
Mass (Da):93,698
Last modified:July 27, 2011 - v3
Checksum:i4724A0F477D304B5
GO
Isoform 2 (identifier: P18052-2) [UniParc]FASTAAdd to Basket

Also known as: Short

The sequence of this isoform differs from the canonical sequence as follows:
     268-268: F → Y
     269-303: Missing.

Show »
Length:794
Mass (Da):89,957
Checksum:i5A282BF9347D18A8
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei268 – 2681F → Y in isoform 2. VSP_011880
Alternative sequencei269 – 30335Missing in isoform 2. VSP_005146Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti231 – 2311L → I in AAA39448. 1 Publication
Sequence conflicti326 – 3261S → G1 Publication
Sequence conflicti411 – 4111C → S1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M36033 mRNA. Translation: AAA39448.1.
M36034 Genomic DNA. Translation: AAA39449.2.
L13607 Genomic DNA. No translation available.
AL731707 Genomic DNA. Translation: CAM13265.1.
CH466519 Genomic DNA. Translation: EDL28272.1.
Z23054 mRNA. Translation: CAA80589.1.
Z23055 mRNA. Translation: CAA80590.1.
CCDSiCCDS16743.1. [P18052-1]
PIRiA47373.
RefSeqiNP_033006.2. NM_008980.2. [P18052-1]
UniGeneiMm.224246.

Genome annotation databases

EnsembliENSMUST00000077303; ENSMUSP00000076533; ENSMUSG00000027303. [P18052-1]
GeneIDi19262.
KEGGimmu:19262.
UCSCiuc008mjc.2. mouse. [P18052-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M36033 mRNA. Translation: AAA39448.1 .
M36034 Genomic DNA. Translation: AAA39449.2 .
L13607 Genomic DNA. No translation available.
AL731707 Genomic DNA. Translation: CAM13265.1 .
CH466519 Genomic DNA. Translation: EDL28272.1 .
Z23054 mRNA. Translation: CAA80589.1 .
Z23055 mRNA. Translation: CAA80590.1 .
CCDSi CCDS16743.1. [P18052-1 ]
PIRi A47373.
RefSeqi NP_033006.2. NM_008980.2. [P18052-1 ]
UniGenei Mm.224246.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1P15 X-ray 2.00 A/B 577-829 [» ]
1YFO X-ray 2.25 A/B 202-539 [» ]
ProteinModelPortali P18052.
SMRi P18052. Positions 208-821.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 202491. 1 interaction.
IntActi P18052. 12 interactions.
MINTi MINT-129698.

PTM databases

PhosphoSitei P18052.

Proteomic databases

MaxQBi P18052.
PaxDbi P18052.
PRIDEi P18052.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000077303 ; ENSMUSP00000076533 ; ENSMUSG00000027303 . [P18052-1 ]
GeneIDi 19262.
KEGGi mmu:19262.
UCSCi uc008mjc.2. mouse. [P18052-1 ]

Organism-specific databases

CTDi 5786.
MGIi MGI:97808. Ptpra.

Phylogenomic databases

eggNOGi COG5599.
GeneTreei ENSGT00590000082937.
HOGENOMi HOG000231464.
HOVERGENi HBG053758.
InParanoidi A2AHF2.
KOi K18032.
OMAi KRGEENT.
TreeFami TF351829.

Miscellaneous databases

EvolutionaryTracei P18052.
NextBioi 296124.
PROi P18052.
SOURCEi Search...

Gene expression databases

ArrayExpressi P18052.
Bgeei P18052.
CleanExi MM_PTPRA.
Genevestigatori P18052.

Family and domain databases

Gene3Di 3.90.190.10. 3 hits.
InterProi IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
IPR016336. Tyr_Pase_rcpt_a/e-type.
IPR027262. Tyr_Pase_rcpt_alpha.
[Graphical view ]
Pfami PF00102. Y_phosphatase. 2 hits.
[Graphical view ]
PIRSFi PIRSF500808. PTPR_alpha. 1 hit.
PIRSF002006. PTPR_alpha_epsilon. 1 hit.
PRINTSi PR00700. PRTYPHPHTASE.
SMARTi SM00194. PTPc. 2 hits.
[Graphical view ]
SUPFAMi SSF52799. SSF52799. 3 hits.
PROSITEi PS00383. TYR_PHOSPHATASE_1. 2 hits.
PS50056. TYR_PHOSPHATASE_2. 2 hits.
PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of an additional member of the protein-tyrosine-phosphatase family: evidence for alternative splicing in the tyrosine phosphatase domain."
    Matthews R.J., Cahir E.D., Thomas M.L.
    Proc. Natl. Acad. Sci. U.S.A. 87:4444-4448(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
    Strain: C57BL/6 X DBA/2.
  2. "Cloning and expression of a widely expressed receptor tyrosine phosphatase."
    Sap J., D'Eustachio P., Givol D., Schlessinger J.
    Proc. Natl. Acad. Sci. U.S.A. 87:6112-6116(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY.
    Strain: BALB/c.
    Tissue: Brain.
  3. "Leukocyte common antigen-related phosphatase (LRP) gene structure: conservation of the genomic organization of transmembrane protein tyrosine phosphatases."
    Wong E.C., Mullersman J.E., Thomas M.L.
    Genomics 17:33-38(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  5. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "Differential expression of a novel murine non-receptor protein tyrosine phosphatase during differentiation of P19 embryonal carcinoma cells."
    den Hertog J., Pals C.E., Jonk L.J., Kruijer W.
    Biochem. Biophys. Res. Commun. 184:1241-1249(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 322-356.
  7. "Identification of novel protein tyrosine phosphatases of hematopoietic cells by polymerase chain reaction amplification."
    Yi T., Cleveland J.L., Ihle J.N.
    Blood 78:2222-2228(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 358-467, TISSUE SPECIFICITY.
    Strain: BALB/c.
    Tissue: Myeloid leukemia cell.
  8. "A novel receptor-type protein tyrosine phosphatase with a single catalytic domain is specifically expressed in mouse brain."
    Hendriks W., Schepens J., Brugman C., Zeeuwen P., Wieringa B.
    Biochem. J. 305:499-504(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 358-467.
    Strain: BALB/c.
    Tissue: Brain.
  9. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 566-576, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.
  10. "Identification and typing of members of the protein-tyrosine phosphatase gene family expressed in mouse brain."
    Schepens J., Zeeuwen P., Wieringa B., Hendriks W.
    Mol. Biol. Rep. 16:241-248(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 651-756.
    Strain: BALB/c.
    Tissue: Brain.
  11. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
    Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
    Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
    Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
    J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-825, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Mast cell.
  13. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
    Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
    J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-825, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  14. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-202; SER-204 AND TYR-825, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  15. "Structural basis for inhibition of receptor protein-tyrosine phosphatase-alpha by dimerization."
    Bilwes A.M., den Hertog J., Hunter T., Noel J.P.
    Nature 382:555-559(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 202-503.

Entry informationi

Entry nameiPTPRA_MOUSE
AccessioniPrimary (citable) accession number: P18052
Secondary accession number(s): A2AHF2, Q61808
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 145 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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