P18045 (VPX_HV2G1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein Vpx

Alternative name(s):
Viral protein X
X ORF protein

Gene names

Name:

vpx

Organism

Human immunodeficiency virus type 2 subtype A (isolate Ghana-1) (HIV-2) [Complete proteome]

Taxonomic identifier

11717 [NCBI]

Taxonomic lineage

Viruses › Retro-transcribing viruses › Retroviridae › Orthoretrovirinae › Lentivirus › Primate lentivirus group › Human immunodeficiency virus 2

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	112 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays a role in nuclear translocation of the viral pre-integration complex (PIC), thus is required for the virus to infect non-dividing cells. Targets specific host proteins for degradation by the 26S proteasome. Acts by associating with the cellular CUL4A-DDB1 E3 ligase complex through direct interaction with host VPRPB/DCAF-1. This change in the E3 ligase substrate specificity results in the degradation of host SAMHD1. In turn, SAMHD1 depletion allows viral replication in host myeloid cells by preventing SAMHD1-mediated hydrolysis of intracellular dNTPs necessary for reverse transcription By similarity.
Subunit structure	Interacts with the P6 region of unprocessed GAG. Interacts with host VPRBP/DCAF1, leading to change substrate specificity of the CUL4A-DDB1 E3 ligase complex By similarity.
Subcellular location	Virion. Host nucleus. Note: Nuclear just after virion uncoating, or if expressed in the absence of unprocessed GAG By similarity.
Sequence similarities	Belongs to the lentivirus VPX protein family.

Ontologies

Keywords
Biological process	Host-virus interaction Inhibition of host innate immune response by virus Viral immunoevasion
Cellular component	Host nucleus Virion
Disease	AIDS
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	viral infectious cycle Inferred from electronic annotation. Source: InterPro
Cellular_component	host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 112	112	Protein Vpx		PRO_0000085393
Regions
	Motif	65 – 72	8	Nuclear localization signal By similarity

Sequences

	Sequence		Length	Mass (Da)
	P18045 [UniParc]. Last modified November 1, 1990. Version 1. Checksum: 70998DF097DAA579	FASTA	112	12,820
	10 20 30 40 50 60 MTDPRERVPP GNSGEETIGE AFEWLDRTIE ALNREAVNHL PRELIFQVWQ RSWRYWHDDQ 70 80 90 100 110 GMSPSYTKYR YLCLMQKAVF IHFKRGCTCL GGGHGPGGWR SGPPPPPPPG LV

References

[1]	"Genomic divergence of HIV-2 from Ghana." Hasegawa A., Tsujimoto H., Maki N., Ishikawa K., Miura T., Fukasawa M., Miki K., Hayami M. AIDS Res. Hum. Retroviruses 5:593-604(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M30895 Genomic DNA. Translation: AAA43935.1.
PIR	ASLJGH. JS0330.
3D structure databases
ProteinModelPortal	P18045.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000012. RetroV_VpR/X. [Graphical view]
Pfam	PF00522. VPR. 1 hit. [Graphical view]
PRINTS	PR00444. HIVVPRVPX.
ProtoNet	Search...

Entry information

Entry name

VPX_HV2G1

Accession

Primary (citable) accession number: P18045

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	May 1, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program