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P18036 (NSP5_ROTHB) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Non-structural protein 5

Short name=NSP5
Alternative name(s):
NS26
OrganismRotavirus A (strain Human/Indonesia/B37/198? G8-Px[x]-Ix-Rx-Cx-Mx-Ax-N2-Tx-Ex-H2) (RV-A)
Taxonomic identifier10949 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length198 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in genome replication. Plays a crucial role, together with NSP2, in the formation of virus factories (viroplasms) which are large inclusions in the cytoplasm where core-like replication intermediates are assembled and RNA replication takes place. May regulate NSP2-RNA interactions during genome replication, since NSP5 competes with RNA for the same binding site on the NSP2 octamer. Binds to either ssRNA or dsRNA with similar affinities. Displays ATPase and autokinase activities By similarity.

Cofactor

Magnesium for ATPase activity By similarity.

Subunit structure

Homodimer. Interacts with VP1. Interacts with VP2. Interacts with NSP2; this interaction leads to up-regulation of NSP5 hyperphosphorylation and formation of virus factories. Interacts with NSP6 By similarity.

Subcellular location

Host cytoplasm. Note: Found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging By similarity.

Post-translational modification

O-glycosylated By similarity.

Hyperphosphorylated on serine residues, when in dimeric form. Ser-67 phosphorylation by CK1 is required for the hyperphosphorylation of NSP5 dimer. Impaired phosphorylation is associated with a profound morphological change in virus factories and a moderate decrease in virus replication By similarity.

Sequence similarities

Belongs to the rotavirus A NSP5 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 198198Non-structural protein 5
PRO_0000149633

Regions

Region1 – 4848Interaction with VP1 By similarity
Region189 – 19810Homodimerization and interaction with NSP6 By similarity
Compositional bias2 – 101100Ser-rich

Sites

Metal binding921Magnesium Potential

Amino acid modifications

Modified residue671Phosphoserine; by host CK1 By similarity
Modified residue1541Phosphoserine; by host By similarity
Modified residue1561Phosphoserine; by host By similarity
Modified residue1641Phosphoserine; by host By similarity
Modified residue1661Phosphoserine; by host By similarity

Sequences

Sequence LengthMass (Da)Tools
P18036 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: EA5945DF361D8E64

FASTA19821,779
        10         20         30         40         50         60 
MSLSIDVTSL PSISSSIYKH ESSSTTSTIS GKSIGRSEQY ISPDAEAFNK YMLSKSPEDI 

        70         80         90        100        110        120 
GPSDSASNDP LTSFSIRSNA VKTNPDAGVS MDSSTQSRPS SHIGCDQVDF SLNRGIKIDA 

       130        140        150        160        170        180 
MVDSSISIST KSKKEKSKHE TKNRKCYPKI EAESDSDDYV LDDSDSDDGK CKNCKYKKKY 

       190 
FALRLRMKQV AMQLIENL 

« Hide

References

[1]"Sequences of VP9 genes from short and supershort rotavirus strains."
Nuttall S.D., Hum C.P., Holmes I.H., Dyall-Smith M.L.
Virology 171:453-457(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M28377 Genomic RNA. Translation: AAA57459.1.
PIRMNXRB3. A34007.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR002512. Rotavirus_A/C_NSP5.
[Graphical view]
PfamPF01525. Rota_NS26. 1 hit.
[Graphical view]
PIRSFPIRSF004006. Rota_NS26. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNSP5_ROTHB
AccessionPrimary (citable) accession number: P18036
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: February 1, 1996
Last modified: April 3, 2013
This is version 47 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families