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Protein

Probable E3 ubiquitin-protein ligase ipaH7.8

Gene

ipaH7.8

Organism
Shigella flexneri
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. This protein is an E3 ubiquitin ligase that interferes with host's ubiquitination pathway (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei357 – 3571Glycyl thioester intermediateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway, Virulence

Names & Taxonomyi

Protein namesi
Recommended name:
Probable E3 ubiquitin-protein ligase ipaH7.8 (EC:6.3.2.-)
Gene namesi
Name:ipaH7.8
Ordered Locus Names:CP0078
ORF Names:pWR501_0084, SFLP133
Encoded oniPlasmid pWR1002 Publications
Plasmid pCP3011 Publication
Plasmid pSF51 Publication
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
Proteomesi
  • UP000001006 Componenti: Plasmid pCP301

Subcellular locationi

  • Secreted
  • Host cytoplasm Curated

  • Note: Secreted via Mxi-Spa type III secretion system (TTSS), and delivered into the host cytoplasm.Curated

GO - Cellular componenti

  • extracellular region Source: UniProtKB
  • host cell cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 565565Probable E3 ubiquitin-protein ligase ipaH7.8PRO_0000084218Add
BLAST

Post-translational modificationi

Ubiquitinated in the presence of host E1 ubiquitin-activating enzyme, E2 ubiquitin-conjugating enzyme and ubiquitin.By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiP18014.
PRIDEiP18014.

Interactioni

Protein-protein interaction databases

STRINGi198214.CP0078.

Structurei

Secondary structure

1
565
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi274 – 2796Combined sources
Turni284 – 2863Combined sources
Turni288 – 2903Combined sources
Turni292 – 2943Combined sources
Helixi295 – 2973Combined sources
Helixi303 – 31412Combined sources
Helixi325 – 33814Combined sources
Helixi340 – 35213Combined sources
Beta strandi356 – 3583Combined sources
Helixi361 – 37919Combined sources
Turni380 – 3845Combined sources
Helixi386 – 41025Combined sources
Helixi418 – 42811Combined sources
Turni429 – 4346Combined sources
Helixi452 – 47423Combined sources
Helixi478 – 4869Combined sources
Helixi489 – 51527Combined sources
Helixi524 – 55330Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3CKDX-ray2.65A/B/C274-560[»]
ProteinModelPortaliP18014.
SMRiP18014. Positions 274-554.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati58 – 7922LRR 1Add
BLAST
Repeati80 – 9718LRR 2Add
BLAST
Repeati98 – 11922LRR 3Add
BLAST
Repeati120 – 13718LRR 4Add
BLAST
Repeati138 – 15720LRR 5Add
BLAST
Repeati158 – 17922LRR 6Add
BLAST
Repeati180 – 19920LRR 7Add
BLAST
Repeati202 – 22322LRR 8Add
BLAST
Repeati225 – 24824LRR 9Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 262262Interaction with target proteinsBy similarityAdd
BLAST
Regioni263 – 2708LinkerBy similarity
Regioni271 – 565295E3 ubiquitin-protein ligase catalytic domainBy similarityAdd
BLAST

Domaini

The LRR (leucine-rich repeat) domain forms a slightly curved solenoid and may mediate interaction with target proteins.By similarity

Sequence similaritiesi

Contains 9 LRR (leucine-rich) repeats.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat

Phylogenomic databases

eggNOGiENOG4108QHK. Bacteria.
COG4886. LUCA.
HOGENOMiHOG000290475.
OMAiNEYYLRI.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR032674. LRR_E3_ligase_N.
IPR029487. NEL_dom.
[Graphical view]
PfamiPF14496. NEL. 1 hit.
PF12468. TTSSLRR. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiPS51450. LRR. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18014-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFSVNNTHSS VSCSPSINSN STSNEHYLRI LTEWEKNSSP GEERGIAFNR
60 70 80 90 100
LSQCFQNQEA VLNLSDLNLT SLPELPKHIS ALIVENNKLT SLPKLPAFLK
110 120 130 140 150
ELNADNNRLS VIPELPESLT TLSVRSNQLE NLPVLPNHLT SLFVENNRLY
160 170 180 190 200
NLPALPEKLK FLHVYYNRLT TLPDLPDKLE ILCAQRNNLV TFPQFSDRNN
210 220 230 240 250
IRQKEYYFHF NQITTLPESF SQLDSSYRIN ISGNPLSTRV LQSLQRLTSS
260 270 280 290 300
PDYHGPQIYF SMSDGQQNTL HRPLADAVTA WFPENKQSDV SQIWHAFEHE
310 320 330 340 350
EHANTFSAFL DRLSDTVSAR NTSGFREQVA AWLEKLSASA ELRQQSFAVA
360 370 380 390 400
ADATESCEDR VALTWNNLRK TLLVHQASEG LFDNDTGALL SLGREMFRLE
410 420 430 440 450
ILEDIARDKV RTLHFVDEIE VYLAFQTMLA EKLQLSTAVK EMRFYGVSGV
460 470 480 490 500
TANDLRTAEA MVRSREENEF TDWFSLWGPW HAVLKRTEAD RWAQAEEQKY
510 520 530 540 550
EMLENEYSQR VADRLKASGL SGDADAEREA GAQVMRETEQ QIYRQLTDEV
560
LALRLSENGS RLHHS
Length:565
Mass (Da):64,532
Last modified:July 24, 2007 - v2
Checksum:i9294E03F99189520
GO

Sequence cautioni

The sequence AAA26526 differs from that shown. Reason: Frameshift at position 42. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti527 – 5271E → Q in AAA26526 (PubMed:1690703).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF386526 Genomic DNA. Translation: AAL72346.1.
AF348706 Genomic DNA. Translation: AAK18394.1.
AY879342 Genomic DNA. Translation: AAW64892.1.
AL391753 Genomic DNA. Translation: CAC05787.1.
M32063 Genomic DNA. Translation: AAA26526.1. Frameshift.
PIRiA35149.
RefSeqiNP_085238.1. NC_002698.1.
NP_858211.1. NC_004851.1.
WP_010921637.1. NZ_JTBI01000002.1.
YP_006960361.1. NC_019197.1.
YP_009062469.1. NC_024996.1.

Genome annotation databases

EnsemblBacteriaiAAL72346; AAL72346; SF_p0078.
GeneIDi13917161.
876715.
KEGGipg:13917161.
pg:20467606.
pg:876715.
sfl:CP0078.
PATRICi18722468. VBIShiFle86970_5325.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF386526 Genomic DNA. Translation: AAL72346.1.
AF348706 Genomic DNA. Translation: AAK18394.1.
AY879342 Genomic DNA. Translation: AAW64892.1.
AL391753 Genomic DNA. Translation: CAC05787.1.
M32063 Genomic DNA. Translation: AAA26526.1. Frameshift.
PIRiA35149.
RefSeqiNP_085238.1. NC_002698.1.
NP_858211.1. NC_004851.1.
WP_010921637.1. NZ_JTBI01000002.1.
YP_006960361.1. NC_019197.1.
YP_009062469.1. NC_024996.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3CKDX-ray2.65A/B/C274-560[»]
ProteinModelPortaliP18014.
SMRiP18014. Positions 274-554.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198214.CP0078.

Proteomic databases

PaxDbiP18014.
PRIDEiP18014.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL72346; AAL72346; SF_p0078.
GeneIDi13917161.
876715.
KEGGipg:13917161.
pg:20467606.
pg:876715.
sfl:CP0078.
PATRICi18722468. VBIShiFle86970_5325.

Phylogenomic databases

eggNOGiENOG4108QHK. Bacteria.
COG4886. LUCA.
HOGENOMiHOG000290475.
OMAiNEYYLRI.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR032674. LRR_E3_ligase_N.
IPR029487. NEL_dom.
[Graphical view]
PfamiPF14496. NEL. 1 hit.
PF12468. TTSSLRR. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiPS51450. LRR. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIPA7_SHIFL
AccessioniPrimary (citable) accession number: P18014
Secondary accession number(s): Q7BCM7, Q7BEH5, Q99Q94
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: July 24, 2007
Last modified: September 7, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.