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Protein

Probable E3 ubiquitin-protein ligase ipaH7.8

Gene

ipaH7.8

Organism
Shigella flexneri
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. This protein is an E3 ubiquitin ligase that interferes with host's ubiquitination pathway (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei357Glycyl thioester intermediateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway, Virulence

Names & Taxonomyi

Protein namesi
Recommended name:
Probable E3 ubiquitin-protein ligase ipaH7.8 (EC:6.3.2.-)
Gene namesi
Name:ipaH7.8
Ordered Locus Names:CP0078
ORF Names:pWR501_0084, SFLP133
Encoded oniPlasmid pWR1002 Publications
Plasmid pCP3011 Publication
Plasmid pSF51 Publication
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeShigella
Proteomesi
  • UP000001006 Componenti: Plasmid pCP301

Subcellular locationi

  • Secreted
  • Host cytoplasm Curated

  • Note: Secreted via Mxi-Spa type III secretion system (TTSS), and delivered into the host cytoplasm.Curated

GO - Cellular componenti

  • extracellular region Source: UniProtKB
  • host cell cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000842181 – 565Probable E3 ubiquitin-protein ligase ipaH7.8Add BLAST565

Post-translational modificationi

Ubiquitinated in the presence of host E1 ubiquitin-activating enzyme, E2 ubiquitin-conjugating enzyme and ubiquitin.By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiP18014.
PRIDEiP18014.

Interactioni

Protein-protein interaction databases

STRINGi198214.CP0078.

Structurei

Secondary structure

1565
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi274 – 279Combined sources6
Turni284 – 286Combined sources3
Turni288 – 290Combined sources3
Turni292 – 294Combined sources3
Helixi295 – 297Combined sources3
Helixi303 – 314Combined sources12
Helixi325 – 338Combined sources14
Helixi340 – 352Combined sources13
Beta strandi356 – 358Combined sources3
Helixi361 – 379Combined sources19
Turni380 – 384Combined sources5
Helixi386 – 410Combined sources25
Helixi418 – 428Combined sources11
Turni429 – 434Combined sources6
Helixi452 – 474Combined sources23
Helixi478 – 486Combined sources9
Helixi489 – 515Combined sources27
Helixi524 – 553Combined sources30

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CKDX-ray2.65A/B/C274-560[»]
ProteinModelPortaliP18014.
SMRiP18014.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati58 – 79LRR 1Add BLAST22
Repeati80 – 97LRR 2Add BLAST18
Repeati98 – 119LRR 3Add BLAST22
Repeati120 – 137LRR 4Add BLAST18
Repeati138 – 157LRR 5Add BLAST20
Repeati158 – 179LRR 6Add BLAST22
Repeati180 – 199LRR 7Add BLAST20
Repeati202 – 223LRR 8Add BLAST22
Repeati225 – 248LRR 9Add BLAST24

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 262Interaction with target proteinsBy similarityAdd BLAST262
Regioni263 – 270LinkerBy similarity8
Regioni271 – 565E3 ubiquitin-protein ligase catalytic domainBy similarityAdd BLAST295

Domaini

The LRR (leucine-rich repeat) domain forms a slightly curved solenoid and may mediate interaction with target proteins.By similarity

Sequence similaritiesi

Contains 9 LRR (leucine-rich) repeats.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat

Phylogenomic databases

eggNOGiENOG4108QHK. Bacteria.
COG4886. LUCA.
HOGENOMiHOG000290475.
OMAiNEYYLRI.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR032674. LRR_E3_ligase_N.
IPR029487. NEL_dom.
[Graphical view]
PfamiPF14496. NEL. 1 hit.
PF12468. TTSSLRR. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiPS51450. LRR. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18014-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFSVNNTHSS VSCSPSINSN STSNEHYLRI LTEWEKNSSP GEERGIAFNR
60 70 80 90 100
LSQCFQNQEA VLNLSDLNLT SLPELPKHIS ALIVENNKLT SLPKLPAFLK
110 120 130 140 150
ELNADNNRLS VIPELPESLT TLSVRSNQLE NLPVLPNHLT SLFVENNRLY
160 170 180 190 200
NLPALPEKLK FLHVYYNRLT TLPDLPDKLE ILCAQRNNLV TFPQFSDRNN
210 220 230 240 250
IRQKEYYFHF NQITTLPESF SQLDSSYRIN ISGNPLSTRV LQSLQRLTSS
260 270 280 290 300
PDYHGPQIYF SMSDGQQNTL HRPLADAVTA WFPENKQSDV SQIWHAFEHE
310 320 330 340 350
EHANTFSAFL DRLSDTVSAR NTSGFREQVA AWLEKLSASA ELRQQSFAVA
360 370 380 390 400
ADATESCEDR VALTWNNLRK TLLVHQASEG LFDNDTGALL SLGREMFRLE
410 420 430 440 450
ILEDIARDKV RTLHFVDEIE VYLAFQTMLA EKLQLSTAVK EMRFYGVSGV
460 470 480 490 500
TANDLRTAEA MVRSREENEF TDWFSLWGPW HAVLKRTEAD RWAQAEEQKY
510 520 530 540 550
EMLENEYSQR VADRLKASGL SGDADAEREA GAQVMRETEQ QIYRQLTDEV
560
LALRLSENGS RLHHS
Length:565
Mass (Da):64,532
Last modified:July 24, 2007 - v2
Checksum:i9294E03F99189520
GO

Sequence cautioni

The sequence AAA26526 differs from that shown. Reason: Frameshift at position 42.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti527E → Q in AAA26526 (PubMed:1690703).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF386526 Genomic DNA. Translation: AAL72346.1.
AF348706 Genomic DNA. Translation: AAK18394.1.
AY879342 Genomic DNA. Translation: AAW64892.1.
AL391753 Genomic DNA. Translation: CAC05787.1.
M32063 Genomic DNA. Translation: AAA26526.1. Frameshift.
PIRiA35149.
RefSeqiNP_085238.1. NC_002698.1.
NP_858211.1. NC_004851.1.
WP_010921637.1. NZ_JTBI01000002.1.
YP_006960361.1. NC_019197.1.
YP_009062469.1. NC_024996.1.

Genome annotation databases

EnsemblBacteriaiAAL72346; AAL72346; SF_p0078.
GeneIDi1238049.
13917161.
876715.
KEGGisfl:CP0078.
PATRICi18722468. VBIShiFle86970_5325.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF386526 Genomic DNA. Translation: AAL72346.1.
AF348706 Genomic DNA. Translation: AAK18394.1.
AY879342 Genomic DNA. Translation: AAW64892.1.
AL391753 Genomic DNA. Translation: CAC05787.1.
M32063 Genomic DNA. Translation: AAA26526.1. Frameshift.
PIRiA35149.
RefSeqiNP_085238.1. NC_002698.1.
NP_858211.1. NC_004851.1.
WP_010921637.1. NZ_JTBI01000002.1.
YP_006960361.1. NC_019197.1.
YP_009062469.1. NC_024996.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CKDX-ray2.65A/B/C274-560[»]
ProteinModelPortaliP18014.
SMRiP18014.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198214.CP0078.

Proteomic databases

PaxDbiP18014.
PRIDEiP18014.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL72346; AAL72346; SF_p0078.
GeneIDi1238049.
13917161.
876715.
KEGGisfl:CP0078.
PATRICi18722468. VBIShiFle86970_5325.

Phylogenomic databases

eggNOGiENOG4108QHK. Bacteria.
COG4886. LUCA.
HOGENOMiHOG000290475.
OMAiNEYYLRI.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR032674. LRR_E3_ligase_N.
IPR029487. NEL_dom.
[Graphical view]
PfamiPF14496. NEL. 1 hit.
PF12468. TTSSLRR. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiPS51450. LRR. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIPA7_SHIFL
AccessioniPrimary (citable) accession number: P18014
Secondary accession number(s): Q7BCM7, Q7BEH5, Q99Q94
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: July 24, 2007
Last modified: November 30, 2016
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.