Reviewed, UniProtKB/Swiss-Prot P18011 (IPAB_SHIFL)

Names and origin

Protein names

Recommended name:
    Invasin ipaB
Alternative name(s):
    62 kDa antigen

Gene names

Name:	ipaB
Ordered Locus Names:	CP0128

Encoded on

Plasmid pWR100 Ref.1 Ref.2 Ref.3 Ref.4
Plasmid pMYSH6000 Ref.5
Plasmid pCP301 Ref.6

Organism

Shigella flexneri [Complete proteome] [HAMAP]

Taxonomic identifier

623 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	580 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Forms a pore with ipaC, which is inserted into the host cell membrane through the Mxi/Spa apparatus, during cell contact. This pore probably allows the translocation of ipaA. IpaB has also been found to be necessary and sufficient to activate macrophage apoptosis by binding to interleukin-1 beta converting enzyme (ICE). Has also been shown to be important, along with ipaD, to block or regulate secretion through the Mxi/Spa translocon in the presence or absence of the secretion signal, respectively. Ref.7
Subcellular location	Secreted. Host cell membrane; Multi-pass membrane protein Potential. Note: Secreted through the specialized type-III secretion system Mxi/Spa. Inserted into the host cell membrane. Also secreted into the host cell cytoplasm after the escape of bacteria from phagosome, where it colocalizes with ICE.
Induction	Synthesis of this immunogen is repressed at 30 degrees Celsius and restored at 37 degrees Celsius.
Sequence similarities	Belongs to the invasin protein B family.

Ontologies

Keywords
Biological process	Virulence
Cellular component	Cell membrane Membrane Secreted
Domain	Transmembrane
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	host cell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Casp1	P29452	3	EBI-490239,EBI-489700	From a different organism.
CD44	P16070	4	EBI-490239,EBI-490245	From a different organism.
MAD2L2	Q9UI95	4	EBI-490239,EBI-77889	From a different organism.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 580	580	Invasin ipaB		PRO_0000219853
Regions
	Transmembrane	313 – 333	21	Potential
	Transmembrane	399 – 419	21	Potential
Natural variations
	Natural variant	18	1	T → A in plasmid pMYSH6000 and plasmid pCP301.
Experimental info
	Sequence conflict	167	1	E → N in AAA26522. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P18011-1 [UniParc]. Last modified February 1, 1994. Version 2. Checksum: 56325105E4BC0F70	FASTA	580	62,201
	10 20 30 40 50 60 MHNVSTTTTG FPLAKILTST ELGDNTIQAA NDAANKLFSL TIADLTANQN INTTNAHSTS 70 80 90 100 110 120 NILIPELKAP KSLNASSQLT LLIGNLIQIL GEKSLTALTN KITAWKSQQQ ARQQKNLEFS 130 140 150 160 170 180 DKINTLLSET EGLTRDYEKQ INKLKNADSK IKDLENKINQ IQTRLSELDP ESPEKKKLSR 190 200 210 220 230 240 EEIQLTIKKD AAVKDRTLIE QKTLSIHSKL TDKSMQLEKE IDSFSAFSNT ASAEQLSTQQ 250 260 270 280 290 300 KSLTGLASVT QLMATFIQLV GKNNEESLKN DLALFQSLQE SRKTEMERKS DEYAAEVRKA 310 320 330 340 350 360 EELNRVMGCV GKILGALLTI VSVVAAAFSG GASLALAAVG LALMVTDAIV QAATGNSFME 370 380 390 400 410 420 QALNPIMKAV IEPLIKLLSD AFTKMLEGLG VDSKKAKMIG SILGAIAGAL VLVAAVVLVA 430 440 450 460 470 480 TVGKQAAAKL AENIGKIIGK TLTDLIPKFL KNFSSQLDDL ITNAVARLNK FLGAAGDEVI 490 500 510 520 530 540 SKQIISTHLN QAVLLGESVN SATQAGGSVA SAVFQNSAST NLADLTLSKY QVEQLSKYIS 550 560 570 580 EAIEKFGQLQ EVIADLLASM SNSQANRTDV AKAILQQTTA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the invasion plasmid antigen B and C genes (ipaB and ipaC) of Shigella flexneri." Baudry B., Kaczorek M., Sansonetti P.J. Microb. Pathog. 4:345-357(1988) [PubMed: 3071655] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: M90T / Serotype 5a.
[2]	"Characterization of invasion plasmid antigen genes (ipaBCD) from Shigella flexneri." Venkatesan M.M., Buysse J.M., Kopecko D.J. Proc. Natl. Acad. Sci. U.S.A. 85:9317-9321(1988) [PubMed: 3057506] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: M90T / Serotype 5a.
[3]	"The virulence plasmid pWR100 and the repertoire of proteins secreted by the type III secretion apparatus of Shigella flexneri." Buchrieser C., Glaser P., Rusniok C., Nedjari H., d'Hauteville H., Kunst F., Sansonetti P.J., Parsot C. Mol. Microbiol. 38:760-771(2000) [PubMed: 11115111] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: M90T / Serotype 5a.
[4]	"Complete DNA sequence and analysis of the large virulence plasmid of Shigella flexneri." Venkatesan M.M., Goldberg M.B., Rose D.J., Grotbeck E.J., Burland V., Blattner F.R. Infect. Immun. 69:3271-3285(2001) [PubMed: 11292750] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: M90T / Serotype 5a.
[5]	"Functional organization and nucleotide sequence of virulence region-2 on the large virulence plasmid in Shigella flexneri 2a." Sasakawa C., Adler B., Tobe T., Okada N., Nagai S., Komatsu K., Yoshikawa M. Mol. Microbiol. 3:1191-1201(1989) [PubMed: 2552264] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: YSH6000 / Serotype 2a.
[6]	"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J. Nucleic Acids Res. 30:4432-4441(2002) [PubMed: 12384590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a.
[7]	"IpaB, a Shigella flexneri invasin, colocalizes with interleukin-1 beta-converting enzyme in the cytoplasm of macrophages." Thirumalai K., Kim K.-S., Zychlinsky A. Infect. Immun. 65:787-793(1997) [PubMed: 9009343] [Abstract] Cited for: FUNCTION IN APOPTOSIS.
[8]	"Bacterial signals and cell responses during Shigella entry into epithelial cells." Tran Van Nhieu G., Bourdet-Sicard R., Dumenil G., Blocker A., Sansonetti P.J. Cell. Microbiol. 2:187-193(2000) [PubMed: 11207575] [Abstract] Cited for: REVIEW.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J04117 Genomic DNA. Translation: AAA26522.1. M34849 Genomic DNA. Translation: AAA98424.1. AL391753 Genomic DNA. Translation: CAC05803.1. AF348706 Genomic DNA. Translation: AAK18446.1. X15319 Genomic DNA. Translation: CAA33381.1. AF386526 Genomic DNA. Translation: AAL72352.1.
PIR	A34965.
RefSeq	NP_085290.1. NP_858261.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P18011. 4 interactions.
Genome annotation databases
GeneID	1238055. 876451.
GenomeReviews	Gene locus CP0128 in contig AF386526_GR.
KEGG	sfl:CP0128.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P18011.
Family and domain databases
InterPro	IPR003895. Invas_B. [Graphical view]
Pfam	PF03518. Invas_B. 1 hit. [Graphical view]
PRINTS	PR01375. BACINVASINB.
ProtoNet	Search...

Entry information

Entry name

IPAB_SHIFL

Accession

Primary (citable) accession number: P18011

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	February 1, 1994
Last modified:	June 16, 2009
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)