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Protein

Invasin IpaB

Gene

ipaB

Organism
Shigella flexneri
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. Forms a pore with IpaC, which is inserted into the host cell membrane through the Mxi/Spa apparatus, during cell contact. This pore probably allows the translocation of IpaA. IpaB has also been found to be necessary and sufficient to activate macrophage apoptosis by binding to interleukin-1 beta converting enzyme (ICE). Has also been shown to be important, along with IpaD, to block or regulate secretion through the Mxi/Spa translocon in the presence or absence of the secretion signal, respectively. Through interaction with host human MAD2L2, constitutively activates the anaphase-promoting complex APC and induces a cell cycle arrest to prevent epithelial renewal in order to promote bacterial colonization.2 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Virulence

Names & Taxonomyi

Protein namesi
Recommended name:
Invasin IpaB
Alternative name(s):
62 kDa antigen
Gene namesi
Name:ipaB
Ordered Locus Names:CP0128
Encoded oniPlasmid pWR1004 Publications
Plasmid pMYSH60001 Publication
Plasmid pCP3011 Publication
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeShigella
Proteomesi
  • UP000001006 Componenti: Plasmid pCP301

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei313 – 333HelicalSequence analysisAdd BLAST21
Transmembranei399 – 419HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Host nucleus, Membrane, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi61N → A: Loss of interaction with human MAD2L2. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002198531 – 580Invasin IpaBAdd BLAST580

Proteomic databases

PaxDbiP18011.
PRIDEiP18011.

Expressioni

Inductioni

Synthesis of this immunogen is repressed at 30 degrees Celsius and restored at 37 degrees Celsius.

Interactioni

Subunit structurei

Interacts with host human MAD2L2; in the G2/M phase of the cell cycle.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Casp1P294523EBI-490239,EBI-489700From a different organism.
CD44P160704EBI-490239,EBI-490245From a different organism.
MAD2L2Q9UI957EBI-490239,EBI-77889From a different organism.

Protein-protein interaction databases

DIPiDIP-45233N.
IntActiP18011. 4 interactors.
STRINGi198214.CP0128.

Structurei

Secondary structure

1580
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi75 – 90Combined sources16
Helixi93 – 95Combined sources3
Helixi96 – 166Combined sources71
Helixi175 – 222Combined sources48

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3GZ1X-ray2.15P/Q51-72[»]
3GZ2X-ray2.65P16-72[»]
3U0CX-ray2.05A/B28-226[»]
SMRiP18011.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP18011.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni61 – 70Mediates interaction with human MAD2L210

Sequence similaritiesi

Belongs to the invasin protein B family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4106H1J. Bacteria.
ENOG410XWFQ. LUCA.
HOGENOMiHOG000224565.
KOiK13285.

Family and domain databases

InterProiIPR032391. SipB_N.
IPR006972. SseC.
IPR003895. T3SS_invasion_prot_B.
[Graphical view]
PfamiPF04888. SseC. 1 hit.
PF16535. T3SSipB. 1 hit.
[Graphical view]
PRINTSiPR01375. BACINVASINB.

Sequencei

Sequence statusi: Complete.

P18011-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHNVSTTTTG FPLAKILTST ELGDNTIQAA NDAANKLFSL TIADLTANQN
60 70 80 90 100
INTTNAHSTS NILIPELKAP KSLNASSQLT LLIGNLIQIL GEKSLTALTN
110 120 130 140 150
KITAWKSQQQ ARQQKNLEFS DKINTLLSET EGLTRDYEKQ INKLKNADSK
160 170 180 190 200
IKDLENKINQ IQTRLSELDP ESPEKKKLSR EEIQLTIKKD AAVKDRTLIE
210 220 230 240 250
QKTLSIHSKL TDKSMQLEKE IDSFSAFSNT ASAEQLSTQQ KSLTGLASVT
260 270 280 290 300
QLMATFIQLV GKNNEESLKN DLALFQSLQE SRKTEMERKS DEYAAEVRKA
310 320 330 340 350
EELNRVMGCV GKILGALLTI VSVVAAAFSG GASLALAAVG LALMVTDAIV
360 370 380 390 400
QAATGNSFME QALNPIMKAV IEPLIKLLSD AFTKMLEGLG VDSKKAKMIG
410 420 430 440 450
SILGAIAGAL VLVAAVVLVA TVGKQAAAKL AENIGKIIGK TLTDLIPKFL
460 470 480 490 500
KNFSSQLDDL ITNAVARLNK FLGAAGDEVI SKQIISTHLN QAVLLGESVN
510 520 530 540 550
SATQAGGSVA SAVFQNSAST NLADLTLSKY QVEQLSKYIS EAIEKFGQLQ
560 570 580
EVIADLLASM SNSQANRTDV AKAILQQTTA
Length:580
Mass (Da):62,201
Last modified:February 1, 1994 - v2
Checksum:i56325105E4BC0F70
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti167E → N in AAA26522 (PubMed:3057506).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti18T → A in plasmid pMYSH6000 and plasmid pCP301. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04117 Genomic DNA. Translation: AAA26522.1.
M34849 Genomic DNA. Translation: AAA98424.1.
AL391753 Genomic DNA. Translation: CAC05803.1.
AF348706 Genomic DNA. Translation: AAK18446.1.
X15319 Genomic DNA. Translation: CAA33381.1.
AF386526 Genomic DNA. Translation: AAL72352.1.
PIRiA34965.
RefSeqiNP_085290.1. NC_002698.1.
NP_858261.1. NC_004851.1.
YP_009062485.1. NC_024996.1.

Genome annotation databases

EnsemblBacteriaiAAL72352; AAL72352; SF_p0128.
GeneIDi1238055.
876451.
KEGGisfl:CP0128.
PATRICi18722585. VBIShiFle86970_5383.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04117 Genomic DNA. Translation: AAA26522.1.
M34849 Genomic DNA. Translation: AAA98424.1.
AL391753 Genomic DNA. Translation: CAC05803.1.
AF348706 Genomic DNA. Translation: AAK18446.1.
X15319 Genomic DNA. Translation: CAA33381.1.
AF386526 Genomic DNA. Translation: AAL72352.1.
PIRiA34965.
RefSeqiNP_085290.1. NC_002698.1.
NP_858261.1. NC_004851.1.
YP_009062485.1. NC_024996.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3GZ1X-ray2.15P/Q51-72[»]
3GZ2X-ray2.65P16-72[»]
3U0CX-ray2.05A/B28-226[»]
SMRiP18011.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-45233N.
IntActiP18011. 4 interactors.
STRINGi198214.CP0128.

Proteomic databases

PaxDbiP18011.
PRIDEiP18011.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL72352; AAL72352; SF_p0128.
GeneIDi1238055.
876451.
KEGGisfl:CP0128.
PATRICi18722585. VBIShiFle86970_5383.

Phylogenomic databases

eggNOGiENOG4106H1J. Bacteria.
ENOG410XWFQ. LUCA.
HOGENOMiHOG000224565.
KOiK13285.

Miscellaneous databases

EvolutionaryTraceiP18011.

Family and domain databases

InterProiIPR032391. SipB_N.
IPR006972. SseC.
IPR003895. T3SS_invasion_prot_B.
[Graphical view]
PfamiPF04888. SseC. 1 hit.
PF16535. T3SSipB. 1 hit.
[Graphical view]
PRINTSiPR01375. BACINVASINB.
ProtoNetiSearch...

Entry informationi

Entry nameiIPAB_SHIFL
AccessioniPrimary (citable) accession number: P18011
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: February 1, 1994
Last modified: November 30, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.