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Protein

Invasin IpaB

Gene

ipaB

Organism
Shigella flexneri
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. Forms a pore with IpaC, which is inserted into the host cell membrane through the Mxi/Spa apparatus, during cell contact. This pore probably allows the translocation of IpaA. IpaB has also been found to be necessary and sufficient to activate macrophage apoptosis by binding to interleukin-1 beta converting enzyme (ICE). Has also been shown to be important, along with IpaD, to block or regulate secretion through the Mxi/Spa translocon in the presence or absence of the secretion signal, respectively. Through interaction with host human MAD2L2, constitutively activates the anaphase-promoting complex APC and induces a cell cycle arrest to prevent epithelial renewal in order to promote bacterial colonization.2 Publications

GO - Biological processi

Keywordsi

Biological processVirulence

Names & Taxonomyi

Protein namesi
Recommended name:
Invasin IpaB
Alternative name(s):
62 kDa antigen
Gene namesi
Name:ipaB
Ordered Locus Names:CP0128
Encoded oniPlasmid pWR1004 Publications
Plasmid pMYSH60001 Publication
Plasmid pCP3011 Publication
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeShigella
Proteomesi
  • UP000001006 Componenti: Plasmid pCP301

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei313 – 333HelicalSequence analysisAdd BLAST21
Transmembranei399 – 419HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Host cell membrane, Host membrane, Host nucleus, Membrane, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi61N → A: Loss of interaction with human MAD2L2. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002198531 – 580Invasin IpaBAdd BLAST580

Proteomic databases

PRIDEiP18011

Expressioni

Inductioni

Synthesis of this immunogen is repressed at 30 degrees Celsius and restored at 37 degrees Celsius.

Interactioni

Subunit structurei

Interacts with host human MAD2L2; in the G2/M phase of the cell cycle.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

DIPiDIP-45233N
IntActiP18011, 4 interactors

Structurei

Secondary structure

1580
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi75 – 90Combined sources16
Helixi93 – 95Combined sources3
Helixi96 – 166Combined sources71
Helixi175 – 222Combined sources48

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3GZ1X-ray2.15P/Q51-72[»]
3GZ2X-ray2.65P16-72[»]
3U0CX-ray2.05A/B28-226[»]
SMRiP18011
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP18011

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni61 – 70Mediates interaction with human MAD2L210

Sequence similaritiesi

Belongs to the invasin protein B family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4106H1J Bacteria
ENOG410XWFQ LUCA
HOGENOMiHOG000224565
KOiK13285

Family and domain databases

InterProiView protein in InterPro
IPR032391 SipB_N
IPR006972 SseC
IPR003895 T3SS_invasion_prot_B
PfamiView protein in Pfam
PF04888 SseC, 1 hit
PF16535 T3SSipB, 1 hit
PRINTSiPR01375 BACINVASINB

Sequencei

Sequence statusi: Complete.

P18011-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHNVSTTTTG FPLAKILTST ELGDNTIQAA NDAANKLFSL TIADLTANQN
60 70 80 90 100
INTTNAHSTS NILIPELKAP KSLNASSQLT LLIGNLIQIL GEKSLTALTN
110 120 130 140 150
KITAWKSQQQ ARQQKNLEFS DKINTLLSET EGLTRDYEKQ INKLKNADSK
160 170 180 190 200
IKDLENKINQ IQTRLSELDP ESPEKKKLSR EEIQLTIKKD AAVKDRTLIE
210 220 230 240 250
QKTLSIHSKL TDKSMQLEKE IDSFSAFSNT ASAEQLSTQQ KSLTGLASVT
260 270 280 290 300
QLMATFIQLV GKNNEESLKN DLALFQSLQE SRKTEMERKS DEYAAEVRKA
310 320 330 340 350
EELNRVMGCV GKILGALLTI VSVVAAAFSG GASLALAAVG LALMVTDAIV
360 370 380 390 400
QAATGNSFME QALNPIMKAV IEPLIKLLSD AFTKMLEGLG VDSKKAKMIG
410 420 430 440 450
SILGAIAGAL VLVAAVVLVA TVGKQAAAKL AENIGKIIGK TLTDLIPKFL
460 470 480 490 500
KNFSSQLDDL ITNAVARLNK FLGAAGDEVI SKQIISTHLN QAVLLGESVN
510 520 530 540 550
SATQAGGSVA SAVFQNSAST NLADLTLSKY QVEQLSKYIS EAIEKFGQLQ
560 570 580
EVIADLLASM SNSQANRTDV AKAILQQTTA
Length:580
Mass (Da):62,201
Last modified:February 1, 1994 - v2
Checksum:i56325105E4BC0F70
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti167E → N in AAA26522 (PubMed:3057506).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti18T → A in plasmid pMYSH6000 and plasmid pCP301. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04117 Genomic DNA Translation: AAA26522.1
M34849 Genomic DNA Translation: AAA98424.1
AL391753 Genomic DNA Translation: CAC05803.1
AF348706 Genomic DNA Translation: AAK18446.1
X15319 Genomic DNA Translation: CAA33381.1
AF386526 Genomic DNA Translation: AAL72352.1
PIRiA34965
RefSeqiNP_085290.1, NC_002698.1
NP_858261.1, NC_004851.1
YP_009062485.1, NC_024996.1

Genome annotation databases

EnsemblBacteriaiAAL72352; AAL72352; SF_p0128
GeneIDi1238055
876451
KEGGisfl:CP0128
PATRICifig|198214.7.peg.5383

Similar proteinsi

Entry informationi

Entry nameiIPAB_SHIFL
AccessioniPrimary (citable) accession number: P18011
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: February 1, 1994
Last modified: April 25, 2018
This is version 128 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Plasmid, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health