ipaA

Functionⁱ

Rapidly associates with the first 265 amino acids of vinculin after bacteria-cell contact. This interaction is critical for efficient Shigella uptake. IpaA acts as a potent activator of vinculin and increase its ability to interact with F-actin. The complex IpaA-vinculin induces F-actin depolymerization along with the occasional formation of actin filament bundles.2 Publications

GO - Molecular functionⁱ

actin binding Source: UniProtKB-KW
vinculin binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 10545097

Complete GO annotation...

GO - Biological processⁱ

pathogenesis Source: UniProtKB-KW
positive regulation of actin filament depolymerization
Source: UniProtKB
Inferred from direct assayⁱ
- 10545097

Complete GO annotation...

Keywordsⁱ

Molecular function	Actin-binding
Biological process	Virulence

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Invasin IpaA Alternative name(s): 70 kDa antigen
Gene namesⁱ	Name:ipaA Ordered Locus Names:CP0125
Encoded onⁱ	Plasmid pWR1004 Publications Plasmid pCP3011 Publication
Organismⁱ	Shigella flexneri
Taxonomic identifierⁱ	623 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Shigella
Proteomesⁱ	UP000001006 Componentⁱ: Plasmid pCP301

Subcellular locationⁱ

Secreted

Note:

Secreted through the specialized type-III secretion system Mxi/Spa from the bacterium through the cell cytosol.

GO - Cellular componentⁱ

extracellular region Source: UniProtKB-SubCell

Complete GO annotation...

Keywords - Cellular componentⁱ

Secreted

Pathology & Biotechⁱ

Disruption phenotypeⁱ

Cells are still able to induce low levels of internalization, but are impaired in their ability to enter epithelial cells.1 Publication

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000221448	1 – 633	Invasin IpaAAdd BLAST		633

Proteomic databases

PaxDbⁱ	P18010.
PRIDEⁱ	P18010.

Expressionⁱ

Inductionⁱ

Synthesis of this immunogen is repressed at 30 degrees Celsius and restored at 37 degrees Celsius.

Interactionⁱ

Subunit structureⁱ

The association of the vinculin-IpaA complex with actin occurs via the F-actin binding domain located on the tail of vinculin.

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
VCL	P18206	3	EBI-7640410,EBI-716775	From Homo sapiens.

With

Entry

#Exp.

IntAct

Notes

VCL

P18206

3

EBI-7640410,EBI-716775

From Homo sapiens.

GO - Molecular functionⁱ

actin binding Source: UniProtKB-KW
vinculin binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 10545097

Complete GO annotation...

Protein-protein interaction databases

IntActⁱ	P18010. 1 interactor.
Molecular INTeraction database More...MINTⁱ	MINT-6174234.

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Show more details

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	492 – 507	Combined sources	16
Helixⁱ	566 – 582	Combined sources	17
Helixⁱ	611 – 627	Combined sources	17
Helixⁱ	628 – 630	Combined sources	3

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2GDC	X-ray	2.74	B	623-630	[»]
	2GWW	X-ray	2.72	B	602-631	[»]
	2HSQ	X-ray	3.97	B	565-587	[»]
	2IBF	X-ray	3.20	B/D	563-587	[»]
	3RF3	X-ray	1.61	C/D	488-512	[»]
ProteinModelPortalⁱ	P18010.
SMRⁱ	P18010.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	P18010.

EvolutionaryTraceⁱ

P18010.

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the SipA/IpaA family.Curated

Phylogenomic databases

eggNOGⁱ	ENOG4105MH6. Bacteria. ENOG4111VCG. LUCA.
HOGENOMⁱ	HOG000126669.
KEGG Orthology (KO) More...KOⁱ	K13284.

Family and domain databases

Gene3Dⁱ	1.10.4150.10. 1 hit.
InterProⁱ	View protein in InterPro IPR015138. SipA. IPR023225. SipA_chaperone-bd.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF09052. SipA. 1 hit.
SUPFAMⁱ	SSF140746. SSF140746. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

P18010-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MHNVNNTQAP TFLYKATSPS STEYSELKSK ISDIHSSQTS LKTPASVSEK 
        60         70         80         90        100
ENFATSFNQK CLDFLFSSSG KEDVLRSIYS NSMNAYAKSE ILEFSNVLYS 
       110        120        130        140        150
LVHQNGLNFE NEKGLQKIVA QYSELIIKDK LSQDSAFGPW SAKNKKLHQL 
       160        170        180        190        200
RQNIEHRLAL LAQQHTSGEA LSLGQKLLNT EVSSFIKNNI LAELKLSNET 
       210        220        230        240        250
VSSLKLDDLV DAQAKLAFDS LRNQRKNTID SKGFGIGKLS RDLNTVAVFP 
       260        270        280        290        300
ELLRKVLNDI LEDIKDSHPI QDGLPTPPED MPDGGPTPGA NEKTSQPVIH 
       310        320        330        340        350
YHINNDNRTY DNRVFDNRVY DNSYHENPEN DAQSPTSQTN DLLSRNGNSL 
       360        370        380        390        400
LNPQRALVQK VTSVLPHSIS DTVQTFANNS ALEKVFNHTP DNSDGIGSDL 
       410        420        430        440        450
LTTSSQERSA NNSLSRGHRP LNIQNSSTTP PLHPEGVTSS NDNSSDTTKS 
       460        470        480        490        500
SASLSHRVAS QINKFNSNTD SKVLQTDFLS RNGDTYLTRE TIFEASKKVT 
       510        520        530        540        550
NSLSNLISLI GTKSGTQERE LQEKSKDITK STTEHRINNK LKVTDANIRN 
       560        570        580        590        600
YVTETNADTI DKNHAIYEKA KEVSSALSKV LSKIDDTSAE LLTDDISDLK 
       610        620        630 
NNNDITAENN NIYKAAKDVT TSLSKVLKNI NKD

Length:633

Mass (Da):70,093

Last modified:November 1, 1990 - v1

Checksum:ⁱ2F804F45355E4751

GO

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱ	385	V → A in plasmid pCP301.		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X17628 Genomic DNA. Translation: CAA35624.1. AL391753 Genomic DNA. Translation: CAC05800.1. AF348706 Genomic DNA. Translation: AAK18443.1. AF386526 Genomic DNA. Translation: AAL72353.1. J04117 Genomic DNA. Translation: AAA26525.1.
PIRⁱ	S12763. E31265.
NCBI Reference Sequences More...RefSeqⁱ	NP_085287.1. NC_002698.1. NP_858258.1. NC_004851.1. WP_005063225.1. NZ_MSJZ02000181.1. YP_009062482.1. NC_024996.1.

Genome annotation databases

EnsemblBacteriaⁱ	AAL72353; AAL72353; SF_p0125.
GeneIDⁱ	1238056. 876595.
KEGGⁱ	sfl:CP0125.
PATRICⁱ	fig\|198214.7.peg.5380.

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X17628 Genomic DNA. Translation: CAA35624.1. AL391753 Genomic DNA. Translation: CAC05800.1. AF348706 Genomic DNA. Translation: AAK18443.1. AF386526 Genomic DNA. Translation: AAL72353.1. J04117 Genomic DNA. Translation: AAA26525.1.
PIRⁱ	S12763. E31265.
NCBI Reference Sequences More...RefSeqⁱ	NP_085287.1. NC_002698.1. NP_858258.1. NC_004851.1. WP_005063225.1. NZ_MSJZ02000181.1. YP_009062482.1. NC_024996.1.

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2GDC	X-ray	2.74	B	623-630	[»]
	2GWW	X-ray	2.72	B	602-631	[»]
	2HSQ	X-ray	3.97	B	565-587	[»]
	2IBF	X-ray	3.20	B/D	563-587	[»]
	3RF3	X-ray	1.61	C/D	488-512	[»]
ProteinModelPortalⁱ	P18010.
SMRⁱ	P18010.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

IntActⁱ	P18010. 1 interactor.
Molecular INTeraction database More...MINTⁱ	MINT-6174234.

Proteomic databases

PaxDbⁱ	P18010.
PRIDEⁱ	P18010.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAL72353; AAL72353; SF_p0125.
GeneIDⁱ	1238056. 876595.
KEGGⁱ	sfl:CP0125.
PATRICⁱ	fig\|198214.7.peg.5380.

Phylogenomic databases

eggNOGⁱ	ENOG4105MH6. Bacteria. ENOG4111VCG. LUCA.
HOGENOMⁱ	HOG000126669.
KEGG Orthology (KO) More...KOⁱ	K13284.

Miscellaneous databases

EvolutionaryTraceⁱ	P18010.

EvolutionaryTraceⁱ

P18010.

Family and domain databases

Gene3Dⁱ	1.10.4150.10. 1 hit.
InterProⁱ	View protein in InterPro IPR015138. SipA. IPR023225. SipA_chaperone-bd.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF09052. SipA. 1 hit.
SUPFAMⁱ	SSF140746. SSF140746. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	IPAA_SHIFL
Accessionⁱ	P18010Primary (citable) accession number: P18010 Secondary accession number(s): Q8VSH8
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
	Last sequence update:	November 1, 1990
	Last modified:	July 5, 2017
	This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P18010 (IPAA_SHIFL)

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Invasin IpaA

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

Natural variant

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ