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Protein

Ubiquinone biosynthesis O-methyltransferase

Gene

ubiG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

O-methyltransferase that catalyzes the 2 O-methylation steps in the ubiquinone biosynthetic pathway.UniRule annotation1 Publication

Catalytic activityi

S-adenosyl-L-methionine + 3-demethylubiquinone-n = S-adenosyl-L-homocysteine + ubiquinone-n.UniRule annotation1 Publication
S-adenosyl-L-methionine + 3-(all-trans-polyprenyl)benzene-1,2-diol = S-adenosyl-L-homocysteine + 2-methoxy-6-(all-trans-polyprenyl)phenol.UniRule annotation1 Publication

Pathwayi: ubiquinone biosynthesis

This protein is involved in the pathway ubiquinone biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway ubiquinone biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei44S-adenosyl-L-methionineUniRule annotation1 Publication1
Binding sitei64S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1 Publication1
Binding sitei85S-adenosyl-L-methionineUniRule annotation1 Publication1
Binding sitei129S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1 Publication1

GO - Molecular functioni

GO - Biological processi

  • hyperosmotic salinity response Source: EcoCyc
  • ubiquinone biosynthetic process Source: EcoCyc

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processUbiquinone biosynthesis
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:DHHB-METHYLTRANSFER-MONOMER.
MetaCyc:DHHB-METHYLTRANSFER-MONOMER.
UniPathwayiUPA00232.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquinone biosynthesis O-methyltransferaseUniRule annotation
Alternative name(s):
2-octaprenyl-6-hydroxyphenol methylaseUniRule annotation (EC:2.1.1.222UniRule annotation)
3-demethylubiquinone-8 3-O-methyltransferaseUniRule annotation (EC:2.1.1.64UniRule annotation)
Gene namesi
Name:ubiGUniRule annotation
Synonyms:pufX, yfaB
Ordered Locus Names:b2232, JW2226
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11143. ubiG.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001933791 – 240Ubiquinone biosynthesis O-methyltransferaseAdd BLAST240

Proteomic databases

PaxDbiP17993.
PRIDEiP17993.

Interactioni

Subunit structurei

Homodimer.Curated

Binary interactionsi

WithEntry#Exp.IntActNotes
rplVP611753EBI-559367,EBI-542255

Protein-protein interaction databases

BioGridi4262129. 5 interactors.
DIPiDIP-11070N.
IntActiP17993. 19 interactors.
MINTiMINT-1254635.
STRINGi316385.ECDH10B_2391.

Structurei

Secondary structure

1240
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi14 – 23Combined sources10
Helixi24 – 26Combined sources3
Helixi35 – 52Combined sources18
Beta strandi59 – 63Combined sources5
Helixi69 – 76Combined sources8
Beta strandi80 – 86Combined sources7
Helixi88 – 101Combined sources14
Beta strandi106 – 110Combined sources5
Helixi112 – 118Combined sources7
Beta strandi123 – 130Combined sources8
Helixi132 – 134Combined sources3
Helixi138 – 148Combined sources11
Beta strandi149 – 159Combined sources11
Helixi167 – 172Combined sources6
Helixi174 – 179Combined sources6
Helixi194 – 201Combined sources8
Beta strandi204 – 217Combined sources14
Turni218 – 221Combined sources4
Beta strandi222 – 227Combined sources6
Beta strandi233 – 239Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4KDCX-ray2.09A1-240[»]
4KDRX-ray2.00A1-159[»]
A188-240[»]
5DPMX-ray2.10A1-240[»]
ProteinModelPortaliP17993.
SMRiP17993.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the methyltransferase superfamily. UbiG/COQ3 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107RFD. Bacteria.
COG2227. LUCA.
HOGENOMiHOG000278065.
InParanoidiP17993.
KOiK00568.
PhylomeDBiP17993.

Family and domain databases

HAMAPiMF_00472. UbiG. 1 hit.
InterProiView protein in InterPro
IPR029063. SAM-dependent_MTases.
IPR010233. UbiG_MeTrfase.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR01983. UbiG. 1 hit.

Sequencei

Sequence statusi: Complete.

P17993-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNAEKSPVNH NVDHEEIAKF EAVASRWWDL EGEFKPLHRI NPLRLGYIAE
60 70 80 90 100
RAGGLFGKKV LDVGCGGGIL AESMAREGAT VTGLDMGFEP LQVAKLHALE
110 120 130 140 150
SGIQVDYVQE TVEEHAAKHA GQYDVVTCME MLEHVPDPQS VVRACAQLVK
160 170 180 190 200
PGGDVFFSTL NRNGKSWLMA VVGAEYILRM VPKGTHDVKK FIKPAELLGW
210 220 230 240
VDQTSLKERH ITGLHYNPIT NTFKLGPGVD VNYMLHTQNK
Length:240
Mass (Da):26,555
Last modified:November 1, 1990 - v1
Checksum:iD4EB4707A06F5613
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00544 Genomic DNA. Translation: CAA68610.1.
M87509 Genomic DNA. Translation: AAA24714.1.
U00096 Genomic DNA. Translation: AAC75292.1.
AP009048 Genomic DNA. Translation: BAA16049.1.
PIRiA47682.
RefSeqiNP_416735.1. NC_000913.3.
WP_000990765.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75292; AAC75292; b2232.
BAA16049; BAA16049; BAA16049.
GeneIDi946607.
KEGGiecj:JW2226.
eco:b2232.
PATRICifig|1411691.4.peg.3.

Similar proteinsi

Entry informationi

Entry nameiUBIG_ECOLI
AccessioniPrimary (citable) accession number: P17993
Secondary accession number(s): P76924
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: October 25, 2017
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families