P17989 (GUB_FIBSS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-glucanase
EC=3.2.1.73

Alternative name(s):
1,3-1,4-beta-D-glucan 4-glucanohydrolase
Endo-beta-1,3-1,4 glucanase
Lichenase
Mixed linkage beta-glucanase

Gene names

Ordered Locus Names:

Fisuc_2961, FSU_0226

Organism

Fibrobacter succinogenes (strain ATCC 19169 / S85) [Complete proteome] [HAMAP]

Taxonomic identifier

59374 [NCBI]

Taxonomic lineage

Bacteria › Fibrobacteres › Fibrobacterales › Fibrobacteraceae › Fibrobacter

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds.
Sequence similarities	Belongs to the glycosyl hydrolase 16 family.

Ontologies

Keywords
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	licheninase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 27

27

Ref.1

Chain

28 – 349

322

Beta-glucanase

PRO_0000011793

Regions

Repeat

271 – 277

7

1

Repeat

278 – 284

7

2

Repeat

285 – 291

7

3

Repeat

292 – 298

7

4

Repeat

301 – 307

7

5

Region

271 – 307

37

5 X 7 AA tandem repeats of P-X-S-S-S-S-X

Sites

Active site

79

1

Nucleophile By similarity

Active site

83

1

Proton donor By similarity

Secondary structure

1

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349

Helix Strand Turn

Details...

Beta strand

29 – 39

11

Beta strand

41 – 51

11

Beta strand

58 – 65

8

Turn

67 – 70

4

Beta strand

79 – 85

7

Beta strand

91 – 100

10

Beta strand

103 – 105

3

Beta strand

109 – 112

4

Turn

117 – 119

3

Beta strand

122 – 128

7

Beta strand

130 – 137

8

Beta strand

140 – 147

8

Helix

148 – 152

5

Beta strand

157 – 167

11

Helix

169 – 172

4

Helix

177 – 179

3

Beta strand

182 – 194

13

Turn

196 – 198

3

Beta strand

203 – 211

9

Turn

218 – 220

3

Beta strand

221 – 223

3

Beta strand

233 – 235

3

Helix

237 – 239

3

Beta strand

240 – 243

4

Beta strand

246 – 253

8

Sequences

	Sequence		Length	Mass (Da)
	P17989 [UniParc]. Last modified November 1, 1990. Version 1. Checksum: 16DC4F5BDEFC578A	FASTA	349	37,738
	10 20 30 40 50 60 MNIKKTAVKS ALAVAAAAAA LTTNVSAKDF SGAELYTLEE VQYGKFEARM KMAAASGTVS 70 80 90 100 110 120 SMFLYQNGSE IADGRPWVEV DIEVLGKNPG SFQSNIITGK AGAQKTSEKH HAVSPAADQA 130 140 150 160 170 180 FHTYGLEWTP NYVRWTVDGQ EVRKTEGGQV SNLTGTQGLR FNLWSSESAA WVGQFDESKL 190 200 210 220 230 240 PLFQFINWVK VYKYTPGQGE GGSDFTLDWT DNFDTFDGSR WGKGDWTFDG NRVDLTDKNI 250 260 270 280 290 300 YSRDGMLILA LTRKGQESFN GQVPRDDEPA PQSSSSAPAS SSSVPASSSS VPASSSSAFV 310 320 330 340 PPSSSSATNA IHGMRTTPAV AKEHRNLVNA KGAKVNPNGH KRYRVNFEH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence of a Fibrobacter succinogenes mixed-linkage beta-glucanase (1,3-1,4-beta-D-glucan 4-glucanohydrolase) gene." Teather R.M., Erfle J.D. J. Bacteriol. 172:3837-3841(1990) [PubMed: 2193918] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 28-57.
[2]	"Complete sequence of Fibrobacter succinogenes subsp. succinogenes S85." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Detter J.C., Han C., Tapia R., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Weimer P.J. , Stevenson D.M., Boyum J., Brumm P.I., Mead D. Submitted (OCT-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19169 / S85.
[3]	"Complete sequence of Fibrobacter succinogenes subsp. succinogenes S85." Durkin A.S., Nelson K.E., Morrison M., Forsberg C.W., Wilson D.B., Russell J.B., Cann I.K.O., Mackie R.I., White B.A. Submitted (AUG-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19169 / S85.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M33676 Genomic DNA. Translation: AAA24896.1.
CP001792 Genomic DNA. Translation: ACX76541.1.
CP002158 Genomic DNA. Translation: ADL24776.1.

PIR

A44507.

RefSeq

YP_003251023.1. NC_013410.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1MVE	X-ray	1.70	A	25-266	[»]
1ZM1	X-ray	2.30	A/B	27-267	[»]
2R49	X-ray	2.20	A	26-266	[»]
3H0O	X-ray	1.40	A	27-266	[»]
3HR9	X-ray	1.70	A	26-266	[»]

ProteinModelPortal

P17989.

SMR

P17989. Positions 25-266.

ModBase

Search...

Protein family/group databases

CAZy

GH16. Glycoside Hydrolase Family 16.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

8523608.

GenomeReviews

Gene locus Fisuc_2961 in contig CP001792_GR.
Gene locus FSU_0226 in contig CP002158_GR.

KEGG

fsu:Fisuc_2961.

PATRIC

32145677. VBIFibSuc28982_2886.

Organism-specific databases

CMR

Search...

Phylogenomic databases

OMA

SWQSNII.

ProtClustDB

CLSK956259.

Family and domain databases

InterPro

IPR008264. Beta_glucanase.
IPR008985. ConA-like_lec_gl.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
[Graphical view]

Gene3D

G3DSA:2.60.120.200. ConA_like_subgrp. 1 hit.

Pfam

PF00722. Glyco_hydro_16. 1 hit.
[Graphical view]

PRINTS

PR00737. GLHYDRLASE16.

SUPFAM

SSF49899. ConA_like_lec_gl. 1 hit.

PROSITE

PS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

GUB_FIBSS

Accession

Primary (citable) accession number: P17989
Secondary accession number(s): C9RPK9, D9S550

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	January 25, 2012
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program