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Protein

Beta-glucanase

Gene

Fisuc_2961

Organism
Fibrobacter succinogenes (strain ATCC 19169 / S85)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei79 – 791NucleophilePROSITE-ProRule annotation
Active sitei83 – 831Proton donorPROSITE-ProRule annotation

GO - Molecular functioni

  1. licheninase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BRENDAi3.2.1.73. 771.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucanase (EC:3.2.1.73)
Alternative name(s):
1,3-1,4-beta-D-glucan 4-glucanohydrolase
Endo-beta-1,3-1,4 glucanase
Lichenase
Mixed linkage beta-glucanase
Gene namesi
Ordered Locus Names:Fisuc_2961, FSU_0226
OrganismiFibrobacter succinogenes (strain ATCC 19169 / S85)
Taxonomic identifieri59374 [NCBI]
Taxonomic lineageiBacteriaFibrobacteresFibrobacteralesFibrobacteraceaeFibrobacter
ProteomesiUP000001497 Componenti: Chromosome UP000000517 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 27271 PublicationAdd
BLAST
Chaini28 – 349322Beta-glucanasePRO_0000011793Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi59374.Fisuc_2961.

Structurei

Secondary structure

1
349
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi29 – 3911Combined sources
Beta strandi41 – 5111Combined sources
Beta strandi58 – 658Combined sources
Turni67 – 704Combined sources
Beta strandi72 – 743Combined sources
Beta strandi79 – 857Combined sources
Beta strandi91 – 10010Combined sources
Beta strandi103 – 1053Combined sources
Beta strandi109 – 1124Combined sources
Turni117 – 1193Combined sources
Beta strandi122 – 1287Combined sources
Beta strandi133 – 1375Combined sources
Beta strandi140 – 1456Combined sources
Helixi148 – 1514Combined sources
Beta strandi157 – 16711Combined sources
Helixi169 – 1724Combined sources
Helixi177 – 1793Combined sources
Beta strandi182 – 19413Combined sources
Turni196 – 1983Combined sources
Helixi200 – 2023Combined sources
Beta strandi204 – 2118Combined sources
Turni218 – 2203Combined sources
Beta strandi221 – 2233Combined sources
Beta strandi233 – 2353Combined sources
Helixi237 – 2393Combined sources
Beta strandi240 – 2434Combined sources
Beta strandi246 – 2538Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MVEX-ray1.70A25-266[»]
1ZM1X-ray2.30A/B27-267[»]
2R49X-ray2.20A26-266[»]
3AXDX-ray1.53A/B25-271[»]
3AXEX-ray1.53A25-271[»]
3H0OX-ray1.40A27-266[»]
3HR9X-ray1.70A26-266[»]
SMRiP17989. Positions 25-266.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP17989.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati271 – 27771
Repeati278 – 28472
Repeati285 – 29173
Repeati292 – 29874
Repeati301 – 30775

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni271 – 307375 X 7 AA tandem repeats of P-X-S-S-S-S-XAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 16 family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiCOG2273.
OMAiTYKNGSE.
OrthoDBiEOG6JB18P.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR008264. Beta_glucanase.
IPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
PRINTSiPR00737. GLHYDRLASE16.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17989-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIKKTAVKS ALAVAAAAAA LTTNVSAKDF SGAELYTLEE VQYGKFEARM
60 70 80 90 100
KMAAASGTVS SMFLYQNGSE IADGRPWVEV DIEVLGKNPG SFQSNIITGK
110 120 130 140 150
AGAQKTSEKH HAVSPAADQA FHTYGLEWTP NYVRWTVDGQ EVRKTEGGQV
160 170 180 190 200
SNLTGTQGLR FNLWSSESAA WVGQFDESKL PLFQFINWVK VYKYTPGQGE
210 220 230 240 250
GGSDFTLDWT DNFDTFDGSR WGKGDWTFDG NRVDLTDKNI YSRDGMLILA
260 270 280 290 300
LTRKGQESFN GQVPRDDEPA PQSSSSAPAS SSSVPASSSS VPASSSSAFV
310 320 330 340
PPSSSSATNA IHGMRTTPAV AKEHRNLVNA KGAKVNPNGH KRYRVNFEH
Length:349
Mass (Da):37,738
Last modified:November 1, 1990 - v1
Checksum:i16DC4F5BDEFC578A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33676 Genomic DNA. Translation: AAA24896.1.
CP001792 Genomic DNA. Translation: ACX76541.1.
CP002158 Genomic DNA. Translation: ADL24776.1.
PIRiA44507.
RefSeqiWP_014545066.1. NC_017448.1.
YP_003251023.1. NC_013410.1.
YP_005820380.1. NC_017448.1.

Genome annotation databases

EnsemblBacteriaiACX76541; ACX76541; Fisuc_2961.
ADL24776; ADL24776; FSU_0226.
KEGGifsc:FSU_0226.
fsu:Fisuc_2961.
PATRICi32145677. VBIFibSuc28982_2886.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33676 Genomic DNA. Translation: AAA24896.1.
CP001792 Genomic DNA. Translation: ACX76541.1.
CP002158 Genomic DNA. Translation: ADL24776.1.
PIRiA44507.
RefSeqiWP_014545066.1. NC_017448.1.
YP_003251023.1. NC_013410.1.
YP_005820380.1. NC_017448.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MVEX-ray1.70A25-266[»]
1ZM1X-ray2.30A/B27-267[»]
2R49X-ray2.20A26-266[»]
3AXDX-ray1.53A/B25-271[»]
3AXEX-ray1.53A25-271[»]
3H0OX-ray1.40A27-266[»]
3HR9X-ray1.70A26-266[»]
SMRiP17989. Positions 25-266.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi59374.Fisuc_2961.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACX76541; ACX76541; Fisuc_2961.
ADL24776; ADL24776; FSU_0226.
KEGGifsc:FSU_0226.
fsu:Fisuc_2961.
PATRICi32145677. VBIFibSuc28982_2886.

Phylogenomic databases

eggNOGiCOG2273.
OMAiTYKNGSE.
OrthoDBiEOG6JB18P.

Enzyme and pathway databases

BRENDAi3.2.1.73. 771.

Miscellaneous databases

EvolutionaryTraceiP17989.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR008264. Beta_glucanase.
IPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
PRINTSiPR00737. GLHYDRLASE16.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "DNA sequence of a Fibrobacter succinogenes mixed-linkage beta-glucanase (1,3-1,4-beta-D-glucan 4-glucanohydrolase) gene."
    Teather R.M., Erfle J.D.
    J. Bacteriol. 172:3837-3841(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 28-57.
  2. "Complete sequence of Fibrobacter succinogenes subsp. succinogenes S85."
    US DOE Joint Genome Institute
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Detter J.C., Han C., Tapia R., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Weimer P.J.
    , Stevenson D.M., Boyum J., Brumm P.I., Mead D.
    Submitted (OCT-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 19169 / S85.
  3. "Complete sequence of Fibrobacter succinogenes subsp. succinogenes S85."
    Durkin A.S., Nelson K.E., Morrison M., Forsberg C.W., Wilson D.B., Russell J.B., Cann I.K.O., Mackie R.I., White B.A.
    Submitted (AUG-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 19169 / S85.

Entry informationi

Entry nameiGUB_FIBSS
AccessioniPrimary (citable) accession number: P17989
Secondary accession number(s): C9RPK9, D9S550
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: April 1, 2015
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.