ID   NAR2A_RAT               Reviewed;         275 AA.
AC   P17982;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1990, sequence version 1.
DT   13-OCT-2009, entry version 85.
DE   RecName: Full=T-cell ecto-ADP-ribosyltransferase 1;
DE            EC=2.4.2.31;
DE   AltName: Full=T-cell NAD(P)(+)--arginine ADP-ribosyltransferase 1;
DE   AltName: Full=T-cell mono(ADP-ribosyl)transferase 1;
DE   AltName: Full=Alloantigen Rt6.1;
DE   AltName: Full=T-cell surface protein Rt6.1;
DE   Flags: Precursor;
GN   Name=Art2a; Synonyms=Rt6, Rt6-a;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Lewis A;
RX   MEDLINE=90192088; PubMed=2129547; DOI=10.1093/nar/18.4.1047;
RA   Haag F., Koch F., Thiele H.-G.;
RT   "Nucleotide and deduced amino acid sequence of the rat T-cell
RT   alloantigen RT6.1.";
RL   Nucleic Acids Res. 18:1047-1047(1990).
RN   [2]
RP   MUTAGENESIS OF GLN-207.
RX   MEDLINE=96275529; PubMed=8690084; DOI=10.1016/0014-5793(96)00568-6;
RA   Maehama T., Hoshino S., Katada T.;
RT   "Increase in ADP-ribosyltransferase activity of rat T lymphocyte
RT   alloantigen RT6.1 by a single amino acid mutation.";
RL   FEBS Lett. 388:189-191(1996).
CC   -!- FUNCTION: Has NAD+ glycohydrolase activity and extremely low ADP-
CC       ribosyltransferase activity.
CC   -!- CATALYTIC ACTIVITY: NAD(+) + protein-L-arginine = nicotinamide +
CC       N(omega)-(ADP-D-ribosyl)-protein-L-arginine.
CC   -!- CATALYTIC ACTIVITY: NADP(+) + protein-L-arginine = nicotinamide +
CC       N(omega)-((2'-phospho-ADP)-D-ribosyl)-protein-L-arginine.
CC   -!- CATALYTIC ACTIVITY: NAD(+) + H(2)O = nicotinamide + ADP-ribose.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor.
CC   -!- TISSUE SPECIFICITY: Postthymic T-cells.
CC   -!- SIMILARITY: Belongs to the Arg-specific ADP-ribosyltransferase
CC       family.
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DR   EMBL; X52082; CAA36301.1; -; mRNA.
DR   IPI; IPI00193034; -.
DR   PIR; S08464; S08464.
DR   UniGene; Rn.107075; -.
DR   UniGene; Rn.214239; -.
DR   HSSP; P20974; 1GXY.
DR   SMR; P17982; 24-246.
DR   STRING; P17982; -.
DR   Ensembl; ENSRNOT00000026644; ENSRNOP00000026644; ENSRNOG00000019687; Rattus norvegicus.
DR   Ensembl; ENSRNOT00000055260; ENSRNOP00000052135; ENSRNOG00000019687; Rattus norvegicus.
DR   RGD; 3521; Rt6.
DR   HOVERGEN; P17982; -.
DR   BRENDA; 2.4.2.31; 248.
DR   ArrayExpress; P17982; -.
DR   Genevestigator; P17982; -.
DR   GermOnline; ENSRNOG00000019687; Rattus norvegicus.
DR   GO; GO:0031225; C:anchored to membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR   GO; GO:0003956; F:NAD(P)+-protein-arginine ADP-ribosyltransfe...; IEA:EC.
DR   GO; GO:0006471; P:protein amino acid ADP-ribosylation; IEA:InterPro.
DR   InterPro; IPR000768; ART.
DR   PANTHER; PTHR10339; ART; 1.
DR   Pfam; PF01129; ART; 1.
DR   PRINTS; PR00970; RIBTRNSFRASE.
DR   PROSITE; PS01291; ART; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Disulfide bond; Glycoprotein; Glycosyltransferase;
KW   GPI-anchor; Lipoprotein; Membrane; NAD; NADP; Signal; Transferase.
FT   SIGNAL        1     20
FT   CHAIN        21    246       T-cell ecto-ADP-ribosyltransferase 1.
FT                                /FTId=PRO_0000019319.
FT   PROPEP      247    275       Removed in mature form (By similarity).
FT                                /FTId=PRO_0000019320.
FT   ACT_SITE    216    216       By similarity.
FT   BINDING      98     98       NAD (By similarity).
FT   BINDING     146    146       NAD (By similarity).
FT   BINDING     164    164       NAD (By similarity).
FT   BINDING     202    202       NAD (By similarity).
FT   LIPID       246    246       GPI-anchor amidated serine (By
FT                                similarity).
FT   CARBOHYD     58     58       N-linked (GlcNAc...) (Potential).
FT   DISULFID     41    243       By similarity.
FT   DISULFID    141    193       By similarity.
FT   MUTAGEN     207    207       Q->E: Increased ADP-ribosyltransferase
FT                                activity.
SQ   SEQUENCE   275 AA;  31388 MW;  D84CBE8A4704031E CRC64;
     MPSNICKFFL TWWLIQQVTG LTGPLMLDTA PNAFDDQYEG CVNKMEEKAP LLLKEDFNKS
     EKLKVAWEEA KKRWNNIKPS MSYPKGFNDF HGTALVAYTG SIGVDFNRAV REFKENPGQF
     HYKAFHYYLT RALQLLSNGD CHSVYRGTKT RFHYTGAGSV RFGQFTSSSL SKTVAQSPEF
     FSDDGTLFII KTCLGVYIKE FSFYPDQEEV LIPGYEVYQK VRTQGYNEIF LDSPKRKKSN
     YNCLYSSAGT RESCVSLFLV VLTSLLVQLL CLAEP
//