Reviewed, UniProtKB/Swiss-Prot P17981 (NAR2A_MOUSE)

Names and origin

Protein names

Recommended name:
    T-cell ecto-ADP-ribosyltransferase 1
    EC=2.4.2.31
Alternative name(s):
    T-cell NAD(P)(+)--arginine ADP-ribosyltransferase 1
    T-cell mono(ADP-ribosyl)transferase 1
    T-cell differentiation marker Rt6 homolog 1

Gene names

Name:	Art2a
Synonyms:	Rt6-1, Rt6.1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	287 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	NAD+ + protein-L-arginine = nicotinamide + N(omega)-(ADP-D-ribosyl)-protein-L-arginine. NADP+ + protein-L-arginine = nicotinamide + N(omega)-((2'-phospho-ADP)-D-ribosyl)-protein-L-arginine.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor.
Tissue specificity	Expressed in spleen and intestine only.
Involvement in disease	A subset of Rt6+ regulatory T-cells may confer protection to autoimmune disease, and failure to develop this subset may result in enhanced susceptibility for autoimmune disease.
Sequence similarities	Belongs to the Arg-specific ADP-ribosyltransferase family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Signal
Ligand	NAD NADP
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein
Gene Ontology (GO)
Biological process	protein amino acid ADP-ribosylation Inferred from electronic annotation. Source: InterPro response to protein stimulus Inferred from mutant phenotype. Source: MGI
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD(P)+-protein-arginine ADP-ribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20
	Chain	21 – 258	238	T-cell ecto-ADP-ribosyltransferase 1		PRO_0000019317
	Propeptide	259 – 287	29	Removed in mature form By similarity		PRO_0000019318
Sites
	Active site	216	1	By similarity
	Binding site	98	1	NAD By similarity
	Binding site	146	1	NAD By similarity
	Binding site	202	1	NAD By similarity
Amino acid modifications
	Lipidation	258	1	GPI-anchor amidated serine By similarity
	Glycosylation	171	1	N-linked (GlcNAc...) Potential
	Glycosylation	256	1	N-linked (GlcNAc...) Potential
	Disulfide bond	41 ↔ 246		By similarity
	Disulfide bond	141 ↔ 193		By similarity
Natural variations
	Natural variant	22 – 24	3	AVP → TGS in strain: NZW.
	Natural variant	26	1	M → T in strain: NZW.
	Natural variant	40	1	G → S in strain: NZW.
Experimental info
	Sequence conflict	5	1	N → K in AAB35402. Ref.2
	Sequence conflict	11	1	T → A in AAB35402. Ref.2
	Sequence conflict	268	1	S → P in CAA37181. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P17981-1 [UniParc]. Last modified August 13, 2002. Version 2. Checksum: 606A7F69943E2692	FASTA	287	32,801
	10 20 30 40 50 60 MPSNNFKFFL TWWLTQQVTG LAVPFMLDMA PNAFDDQYEG CVEDMEKKAP QLLQEDFNMN 70 80 90 100 110 120 EELKLEWEKA EIKWKEIKNC MSYPAGFHDF HGTALVAYTG NIHRSLNEAT REFKINPGNF 130 140 150 160 170 180 HYKAFHYYLT RALQLLSDQG CRSVYRGTNV RFRYTGKGSV RFGHFASSSL NRSVATSSPF 190 200 210 220 230 240 FNGQGTLFII KTCLGAHIKH CSYYTHEEEV LIPGYEVFHK VKTQSVERYI QISLDSPKRK 250 260 270 280 KSNFNCFYSG STQAANVSSL GSRESCVSLF LVVLLGLLVQ QLTLAEP

References

[1]	"Nucleotide and deduced amino acid sequence for the mouse homologue of the rat T-cell differentiation marker RT6." Koch F., Haag F., Thiele H.-G. Nucleic Acids Res. 18:3636-3636(1990) [PubMed: 2362814] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Spleen.
[2]	"Defects in the structure and expression of the genes for the T cell marker Rt6 in NZW and (NZB x NZW)F1 mice." Koch-Nolte F., Klein J., Hollmann C., Kuehl M., Haag F., Gaskins H.R., Leiter E., Thiele H.-G. Int. Immunol. 7:883-890(1995) [PubMed: 7547715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: NZW. Tissue: Spleen.
[3]	"Expression in BALB/c and C57BL/6 mice of Rt6-1 and Rt6-2 ADP-ribosyltransferases that differ in enzymatic activity: C57BL/6 Rt6-1 is a natural transferase knockout." Kanaitsuka T., Bortell R., Stevens L.A., Moss J., Sardinha D., Rajan T.V., Zipris D., Mordes J.P., Greiner D.L., Rossini A.A. J. Immunol. 159:2741-2749(1997) [PubMed: 9300695] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Spleen.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X52991 mRNA. Translation: CAA37181.1. S79913 mRNA. Translation: AAB35402.1. AF016462 mRNA. Translation: AAB71682.1.
IPI	IPI00113911.
PIR	S12738.
RefSeq	NP_031516.1.
UniGene	Mm.439746
3D structure databases
HSSP	HSSP built from PDB template 1GXY based on UniProtKB P20974.
SMR	P17981. Positions 24-249.
ModBase	Search...
Proteomic databases
PRIDE	P17981.
Genome annotation databases
GeneID	11871.
KEGG	mmu:11871.
Organism-specific databases
CTD	11871.
MGI	MGI:107546. Art2a.
Phylogenomic databases
HOVERGEN	P17981.
Enzyme and pathway databases
BRENDA	2.4.2.31. 244.
Gene expression databases
CleanEx	MM_ART2A.
Genevestigator	P17981.
Family and domain databases
InterPro	IPR000768. ART. [Graphical view]
PANTHER	PTHR10339. ART. 1 hit.
Pfam	PF01129. ART. 1 hit. [Graphical view]
PRINTS	PR00970. RIBTRNSFRASE.
PROSITE	PS01291. ART. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	279887.
SOURCE	Search...

Entry information

Entry name

NAR2A_MOUSE

Accession

Primary (citable) accession number: P17981
Secondary accession number(s): O35278, Q64173

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	August 13, 2002
Last modified:	October 13, 2009
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)