26S protease regulatory subunit 6A - Homo sapiens (Human)

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Reviewed, UniProtKB/Swiss-Prot P17980 (PRS6A_HUMAN)

Last modified November 25, 2008. Version 95. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    26S protease regulatory subunit 6A
Alternative name(s):
    Proteasome 26S subunit ATPase 3
    TAT-binding protein 1
      Short name=TBP-1
    Proteasome subunit P50

Gene names

Name:	PSMC3
Synonyms:	TBP1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	439 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex By similarity. In case of HIV-1 infection, suppresses Tat-mediated transactivation.
Subunit structure	May form a heterodimer with a related family member. Interacts with PAAF1. Interacts with HIV-1 Tat.
Subcellular location	CytoplasmPotential. NucleusPotential.
Sequence similarities	Belongs to the AAA ATPase family.

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Ontologies

Keywords
Biological process	Host-virus interaction
Cellular component	Cytoplasm Nucleus Proteasome
Ligand	ATP-binding Nucleotide-binding
PTM	Acetylation Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Inferred from Experiment. Source: Reactome interspecies interaction between organisms Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle Inferred from Experiment. Source: Reactome positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle Inferred from Experiment. Source: Reactome
Cellular component	cytosol Inferred from electronic annotation. Source: UniProtKB-KW nucleus Ref.4 Traceable author statement. Source: ProtInc proteasome complex Traceable author statement. Source: ProtInc
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro transcription coactivator activity Ref.1 Traceable author statement. Source: ProtInc transcription corepressor activity Ref.4 Traceable author statement. Source: ProtInc
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
HTR1E	P28566	1	EBI-359720,EBI-1043151
NDRG1	Q92597	1	EBI-359720,EBI-716486
PAAF1	Q9BRP4	1	EBI-359720,EBI-1056358
PSMC4	P43686	1	EBI-359720,EBI-743997
PSMD10	O75832	1	EBI-359720,EBI-752185
PSMD6	Q15008	1	EBI-359720,EBI-359701

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 439

439

26S protease regulatory subunit 6A

PRO_0000084698

Regions

Nucleotide binding

227 – 234

ATP Potential

Amino acid modifications

Modified residue

N-acetylmethionine

Modified residue

Phosphoserine

Experimental info

Mutagenesis

233

K → H: Loss of function

Mutagenesis

289

D → A: Loss of function

Sequence conflict

356

M → T in BAD96205. Ref.3

Sequence conflict

409

R → A in AAA36666. Ref.1

Sequence conflict

409

R → A Ref.4

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Sequences

Sequence

Length

Mass (Da)

Tools

P17980-1 [UniParc].

Last modified May 10, 2002. Version 3.
Checksum: 0E443465DDDEBB0B
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FASTA

439

49,204

        10         20         30         40         50         60 
MNLLPNIESP VTRQEKMATV WDEAEQDGIG EEVLKMSTEE IIQRTRLLDS EIKIMKSEVL 

        70         80         90        100        110        120 
RVTHELQAMK DKIKENSEKI KVNKTLPYLV SNVIELLDVD PNDQEEDGAN IDLDSQRKGK 

       130        140        150        160        170        180 
CAVIKTSTRQ TYFLPVIGLV DAEKLKPGDL VGVNKDSYLI LETLPTEYDS RVKAMEVDER 

       190        200        210        220        230        240 
PTEQYSDIGG LDKQIQELVE AIVLPMNHKE KFENLGIQPP KGVLMYGPPG TGKTLLARAC 

       250        260        270        280        290        300 
AAQTKATFLK LAGPQLVQMF IGDGAKLVRD AFALAKEKAP SIIFIDELDA IGTKRFDSEK 

       310        320        330        340        350        360 
AGDREVQRTM LELLNQLDGF QPNTQVKVIA ATNRVDILDP ALLRSGRLDR KIEFPMPNEE 

       370        380        390        400        410        420 
ARARIMQIHS RKMNVSPDVN YEELARCTDD FNGAQCKAVC VEAGMIALRR GATELTHEDY 

       430 
MEGILEVQAK KKANLQYYA

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The type 1 human immunodeficiency virus Tat binding protein is a transcriptional activator belonging to an additional family of evolutionarily conserved genes." Ohana B., Moore P.A., Ruben S.M., Southgate C.D., Green M.R., Rosen C.A. Proc. Natl. Acad. Sci. U.S.A. 90:138-142(1993) [PubMed: 8419915] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney, Lung and Peripheral nerve.
[3]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 16-439. Tissue: Adipose tissue.
[4]	"A cDNA for a protein that interacts with the human immunodeficiency virus Tat transactivator." Nelbock P., Dillion P.J., Perkins A., Rosen C.A. Science 248:1650-1653(1990) [PubMed: 2194290] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 36-439, INTERACTION WITH HIV-1 TAT.
[5]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 36-439.
[6]	Lubec G., Afjehi-Sadat L. Submitted (MAR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 156-171, MASS SPECTROMETRY. Tissue: Brain and Cajal-Retzius cell.
[7]	"Identification, purification, and characterization of a PA700-dependent activator of the proteasome." Demartino G.N., Proske R.J., Moomaw C.R., Strong A.A., Song X., Hisamatsu H., Tanaka K., Slaughter C.A. J. Biol. Chem. 271:3112-3118(1996) [PubMed: 8621709] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE.
[8]	"Proteasomal ATPase-associated factor 1 negatively regulates proteasome activity by interacting with proteasomal ATPases." Park Y., Hwang Y.-P., Lee J.-S., Seo S.-H., Yoon S.K., Yoon J.-B. Mol. Cell. Biol. 25:3842-3853(2005) [PubMed: 15831487] [Abstract] Cited for: INTERACTION WITH PAAF1.
[9]	"Mass spectrometric characterization of the affinity-purified human 26S proteasome complex." Wang X., Chen C.-F., Baker P.R., Chen P.-L., Kaiser P., Huang L. Biochemistry 46:3553-3565(2007) [PubMed: 17323924] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-9, ACETYLATION AT MET-1, MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	M34079 mRNA. Translation: AAA36666.1. BC008713 mRNA. Translation: AAH08713.4. BC073165 mRNA. Translation: AAH73165.3. BC106920 mRNA. Translation: AAI06921.1. BC107804 mRNA. Translation: AAI07805.1. Different initiation. AK222485 mRNA. Translation: BAD96205.1. CR456731 mRNA. Translation: CAG33012.1.
PIR	A34832.
RefSeq	NP_002795.2.
UniGene	Hs.250758
3D structure databases
HSSP	HSSP built from PDB template 1IY0 based on UniProtKB Q9LCZ4.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:27555N.
IntAct	P17980.
PTM databases
PhosphoSite	P17980.
2-D gel databases
REPRODUCTION-2DPAGE	IPI00018398.
Proteomic databases
PeptideAtlas	P17980.
Genome annotation databases
Ensembl	ENSG00000165916. Homo sapiens. [Contig view]
GeneID	5702.
KEGG	hsa:5702.
NMPDR	fig\|9606.3.peg.5546.
Organism-specific databases
H-InvDB	HIX0035958.
HGNC	HGNC:9549. PSMC3.
HPA	HPA006065.
MIM	186852. gene.
PharmGKB	PA33894.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOVERGEN	P17980.
Enzyme and pathway databases
Reactome	REACT_11045. Signaling by Wnt. REACT_152. Cell Cycle, Mitotic. REACT_1538. Cell Cycle Checkpoints. REACT_383. DNA Replication. REACT_6185. HIV Infection. REACT_6850. Cdc20:Phospho-APC/C mediated degradation of Cyclin A. REACT_9035. APC/C:Cdh1-mediated degradation of Skp2.
Gene expression databases
ArrayExpress	P17980.
CleanEx	HS_PSMC3.
GermOnline	ENSG00000165916. Homo sapiens.
Family and domain databases
InterPro	IPR005937. 26S_Psome_P45. IPR003593. AAA+_ATPase_core. IPR003959. AAA_ATPase_core. IPR003960. AAA_ATPase_CS. [Graphical view]
Pfam	PF00004. AAA. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01242. 26Sp45. 1 hit.
PROSITE	PS00674. AAA. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	22154.
SOURCE	Search...

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Entry information

Entry name

PRS6A_HUMAN

Accession

Primary (citable) accession number: P17980
Secondary accession number(s): Q3B757

Q96HD3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	May 10, 2002
Last modified:	November 25, 2008
This is version 95 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Keywords

Gene Ontology (GO)

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

2-D gel databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents