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Protein

26S protease regulatory subunit 6A

Gene

PSMC3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity). In case of HIV-1 infection, suppresses Tat-mediated transactivation.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi227 – 234ATPSequence analysis8

GO - Molecular functioni

  • ATPase activity Source: UniProtKB
  • ATP binding Source: UniProtKB-KW
  • proteasome-activating ATPase activity Source: GO_Central
  • TBP-class protein binding Source: GO_Central
  • transcription coactivator activity Source: ProtInc
  • transcription corepressor activity Source: ProtInc

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Host-virus interaction

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS09302-MONOMER.
ReactomeiR-HSA-1169091. Activation of NF-kappaB in B cells.
R-HSA-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-HSA-1236974. ER-Phagosome pathway.
R-HSA-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-HSA-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-HSA-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-HSA-174154. APC/C:Cdc20 mediated degradation of Securin.
R-HSA-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-HSA-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-HSA-180534. Vpu mediated degradation of CD4.
R-HSA-180585. Vif-mediated degradation of APOBEC3G.
R-HSA-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-HSA-195253. Degradation of beta-catenin by the destruction complex.
R-HSA-202424. Downstream TCR signaling.
R-HSA-211733. Regulation of activated PAK-2p34 by proteasome mediated degradation.
R-HSA-2467813. Separation of Sister Chromatids.
R-HSA-2871837. FCERI mediated NF-kB activation.
R-HSA-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-HSA-350562. Regulation of ornithine decarboxylase (ODC).
R-HSA-382556. ABC-family proteins mediated transport.
R-HSA-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-HSA-4608870. Asymmetric localization of PCP proteins.
R-HSA-4641257. Degradation of AXIN.
R-HSA-4641258. Degradation of DVL.
R-HSA-5358346. Hedgehog ligand biogenesis.
R-HSA-5362768. Hh mutants that don't undergo autocatalytic processing are degraded by ERAD.
R-HSA-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-HSA-5607764. CLEC7A (Dectin-1) signaling.
R-HSA-5610780. Degradation of GLI1 by the proteasome.
R-HSA-5610783. Degradation of GLI2 by the proteasome.
R-HSA-5610785. GLI3 is processed to GLI3R by the proteasome.
R-HSA-5632684. Hedgehog 'on' state.
R-HSA-5658442. Regulation of RAS by GAPs.
R-HSA-5668541. TNFR2 non-canonical NF-kB pathway.
R-HSA-5676590. NIK-->noncanonical NF-kB signaling.
R-HSA-5678895. Defective CFTR causes cystic fibrosis.
R-HSA-5687128. MAPK6/MAPK4 signaling.
R-HSA-5689603. UCH proteinases.
R-HSA-5689880. Ub-specific processing proteases.
R-HSA-6798695. Neutrophil degranulation.
R-HSA-68827. CDT1 association with the CDC6:ORC:origin complex.
R-HSA-68949. Orc1 removal from chromatin.
R-HSA-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-HSA-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-HSA-69481. G2/M Checkpoints.
R-HSA-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-HSA-8852276. The role of GTSE1 in G2/M progression after G2 checkpoint.
R-HSA-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
26S protease regulatory subunit 6A
Alternative name(s):
26S proteasome AAA-ATPase subunit RPT5
Proteasome 26S subunit ATPase 3
Proteasome subunit P50
Tat-binding protein 1
Short name:
TBP-1
Gene namesi
Name:PSMC3
Synonyms:TBP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:9549. PSMC3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasmic mRNA processing body Source: UniProtKB
  • cytosol Source: Reactome
  • cytosolic proteasome complex Source: GO_Central
  • membrane Source: UniProtKB
  • nuclear proteasome complex Source: GO_Central
  • nucleoplasm Source: Reactome
  • nucleus Source: UniProtKB
  • proteasome accessory complex Source: UniProtKB
  • proteasome complex Source: UniProtKB
  • proteasome regulatory particle, base subcomplex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi233K → H: Loss of function. 1
Mutagenesisi289D → A: Loss of function. 1

Organism-specific databases

DisGeNETi5702.
OpenTargetsiENSG00000165916.
PharmGKBiPA33894.

Polymorphism and mutation databases

BioMutaiPSMC3.
DMDMi20532406.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000846981 – 43926S protease regulatory subunit 6AAdd BLAST439

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei9PhosphoserineCombined sources1
Modified residuei376PhosphoserineCombined sources1

Post-translational modificationi

Sumoylated by UBE2I in response to MEKK1-mediated stimuli.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP17980.
MaxQBiP17980.
PaxDbiP17980.
PeptideAtlasiP17980.
PRIDEiP17980.

2D gel databases

REPRODUCTION-2DPAGEIPI00018398.

PTM databases

iPTMnetiP17980.
PhosphoSitePlusiP17980.
SwissPalmiP17980.

Miscellaneous databases

PMAP-CutDBP17980.

Expressioni

Gene expression databases

BgeeiENSG00000165916.
CleanExiHS_PSMC3.
ExpressionAtlasiP17980. baseline and differential.
GenevisibleiP17980. HS.

Organism-specific databases

HPAiHPA006065.

Interactioni

Subunit structurei

May form a heterodimer with a related family member. Interacts with PAAF1. Interacts with HIV-1 Tat.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-359720,EBI-359720
A8K2R33EBI-359720,EBI-9977437
P03255-12EBI-359720,EBI-6692439From a different organism.
AMOTL2Q9Y2J44EBI-359720,EBI-746752
AMOTL2Q9Y2J4-45EBI-359720,EBI-10187270
PSMC6P6233314EBI-359720,EBI-357669
PSMD9O0023313EBI-359720,EBI-750973

GO - Molecular functioni

Protein-protein interaction databases

BioGridi111675. 184 interactors.
DIPiDIP-27555N.
IntActiP17980. 48 interactors.
MINTiMINT-1149785.
STRINGi9606.ENSP00000298852.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5GJQelectron microscopy4.50M1-439[»]
5GJRelectron microscopy3.500/M1-439[»]
5L4Gelectron microscopy4.02M1-439[»]
5T0Celectron microscopy3.80AF/BF1-439[»]
5T0Gelectron microscopy4.40F1-439[»]
5T0Helectron microscopy6.80F1-439[»]
5T0Ielectron microscopy8.00F1-439[»]
5T0Jelectron microscopy8.00F1-439[»]
ProteinModelPortaliP17980.
SMRiP17980.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Phylogenomic databases

eggNOGiKOG0652. Eukaryota.
COG1222. LUCA.
GeneTreeiENSGT00730000111070.
HOVERGENiHBG000109.
InParanoidiP17980.
KOiK03065.
OMAiPMKEADR.
OrthoDBiEOG091G07E0.
PhylomeDBiP17980.
TreeFamiTF105648.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR005937. 26S_Psome_P45.
IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01242. 26Sp45. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P17980-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLLPNIESP VTRQEKMATV WDEAEQDGIG EEVLKMSTEE IIQRTRLLDS
60 70 80 90 100
EIKIMKSEVL RVTHELQAMK DKIKENSEKI KVNKTLPYLV SNVIELLDVD
110 120 130 140 150
PNDQEEDGAN IDLDSQRKGK CAVIKTSTRQ TYFLPVIGLV DAEKLKPGDL
160 170 180 190 200
VGVNKDSYLI LETLPTEYDS RVKAMEVDER PTEQYSDIGG LDKQIQELVE
210 220 230 240 250
AIVLPMNHKE KFENLGIQPP KGVLMYGPPG TGKTLLARAC AAQTKATFLK
260 270 280 290 300
LAGPQLVQMF IGDGAKLVRD AFALAKEKAP SIIFIDELDA IGTKRFDSEK
310 320 330 340 350
AGDREVQRTM LELLNQLDGF QPNTQVKVIA ATNRVDILDP ALLRSGRLDR
360 370 380 390 400
KIEFPMPNEE ARARIMQIHS RKMNVSPDVN YEELARCTDD FNGAQCKAVC
410 420 430
VEAGMIALRR GATELTHEDY MEGILEVQAK KKANLQYYA
Length:439
Mass (Da):49,204
Last modified:May 10, 2002 - v3
Checksum:i0E443465DDDEBB0B
GO

Sequence cautioni

The sequence AAI07805 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti356M → T in BAD96205 (Ref. 5) Curated1
Sequence conflicti409R → A in AAA36666 (PubMed:2194290).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK313518 mRNA. Translation: BAG36298.1.
CH471064 Genomic DNA. Translation: EAW67916.1.
BC008713 mRNA. Translation: AAH08713.4.
BC073165 mRNA. Translation: AAH73165.3.
BC106920 mRNA. Translation: AAI06921.1.
BC107804 mRNA. Translation: AAI07805.1. Different initiation.
AK222485 mRNA. Translation: BAD96205.1.
M34079 mRNA. Translation: AAA36666.1.
CR456731 mRNA. Translation: CAG33012.1.
CCDSiCCDS7935.1.
PIRiA34832.
RefSeqiNP_002795.2. NM_002804.4.
UniGeneiHs.250758.

Genome annotation databases

EnsembliENST00000298852; ENSP00000298852; ENSG00000165916.
ENST00000619920; ENSP00000481029; ENSG00000165916.
GeneIDi5702.
KEGGihsa:5702.
UCSCiuc001nfh.2. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK313518 mRNA. Translation: BAG36298.1.
CH471064 Genomic DNA. Translation: EAW67916.1.
BC008713 mRNA. Translation: AAH08713.4.
BC073165 mRNA. Translation: AAH73165.3.
BC106920 mRNA. Translation: AAI06921.1.
BC107804 mRNA. Translation: AAI07805.1. Different initiation.
AK222485 mRNA. Translation: BAD96205.1.
M34079 mRNA. Translation: AAA36666.1.
CR456731 mRNA. Translation: CAG33012.1.
CCDSiCCDS7935.1.
PIRiA34832.
RefSeqiNP_002795.2. NM_002804.4.
UniGeneiHs.250758.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5GJQelectron microscopy4.50M1-439[»]
5GJRelectron microscopy3.500/M1-439[»]
5L4Gelectron microscopy4.02M1-439[»]
5T0Celectron microscopy3.80AF/BF1-439[»]
5T0Gelectron microscopy4.40F1-439[»]
5T0Helectron microscopy6.80F1-439[»]
5T0Ielectron microscopy8.00F1-439[»]
5T0Jelectron microscopy8.00F1-439[»]
ProteinModelPortaliP17980.
SMRiP17980.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111675. 184 interactors.
DIPiDIP-27555N.
IntActiP17980. 48 interactors.
MINTiMINT-1149785.
STRINGi9606.ENSP00000298852.

PTM databases

iPTMnetiP17980.
PhosphoSitePlusiP17980.
SwissPalmiP17980.

Polymorphism and mutation databases

BioMutaiPSMC3.
DMDMi20532406.

2D gel databases

REPRODUCTION-2DPAGEIPI00018398.

Proteomic databases

EPDiP17980.
MaxQBiP17980.
PaxDbiP17980.
PeptideAtlasiP17980.
PRIDEiP17980.

Protocols and materials databases

DNASUi5702.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000298852; ENSP00000298852; ENSG00000165916.
ENST00000619920; ENSP00000481029; ENSG00000165916.
GeneIDi5702.
KEGGihsa:5702.
UCSCiuc001nfh.2. human.

Organism-specific databases

CTDi5702.
DisGeNETi5702.
GeneCardsiPSMC3.
HGNCiHGNC:9549. PSMC3.
HPAiHPA006065.
MIMi186852. gene.
neXtProtiNX_P17980.
OpenTargetsiENSG00000165916.
PharmGKBiPA33894.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0652. Eukaryota.
COG1222. LUCA.
GeneTreeiENSGT00730000111070.
HOVERGENiHBG000109.
InParanoidiP17980.
KOiK03065.
OMAiPMKEADR.
OrthoDBiEOG091G07E0.
PhylomeDBiP17980.
TreeFamiTF105648.

Enzyme and pathway databases

BioCyciZFISH:HS09302-MONOMER.
ReactomeiR-HSA-1169091. Activation of NF-kappaB in B cells.
R-HSA-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-HSA-1236974. ER-Phagosome pathway.
R-HSA-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-HSA-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-HSA-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-HSA-174154. APC/C:Cdc20 mediated degradation of Securin.
R-HSA-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-HSA-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-HSA-180534. Vpu mediated degradation of CD4.
R-HSA-180585. Vif-mediated degradation of APOBEC3G.
R-HSA-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-HSA-195253. Degradation of beta-catenin by the destruction complex.
R-HSA-202424. Downstream TCR signaling.
R-HSA-211733. Regulation of activated PAK-2p34 by proteasome mediated degradation.
R-HSA-2467813. Separation of Sister Chromatids.
R-HSA-2871837. FCERI mediated NF-kB activation.
R-HSA-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-HSA-350562. Regulation of ornithine decarboxylase (ODC).
R-HSA-382556. ABC-family proteins mediated transport.
R-HSA-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-HSA-4608870. Asymmetric localization of PCP proteins.
R-HSA-4641257. Degradation of AXIN.
R-HSA-4641258. Degradation of DVL.
R-HSA-5358346. Hedgehog ligand biogenesis.
R-HSA-5362768. Hh mutants that don't undergo autocatalytic processing are degraded by ERAD.
R-HSA-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-HSA-5607764. CLEC7A (Dectin-1) signaling.
R-HSA-5610780. Degradation of GLI1 by the proteasome.
R-HSA-5610783. Degradation of GLI2 by the proteasome.
R-HSA-5610785. GLI3 is processed to GLI3R by the proteasome.
R-HSA-5632684. Hedgehog 'on' state.
R-HSA-5658442. Regulation of RAS by GAPs.
R-HSA-5668541. TNFR2 non-canonical NF-kB pathway.
R-HSA-5676590. NIK-->noncanonical NF-kB signaling.
R-HSA-5678895. Defective CFTR causes cystic fibrosis.
R-HSA-5687128. MAPK6/MAPK4 signaling.
R-HSA-5689603. UCH proteinases.
R-HSA-5689880. Ub-specific processing proteases.
R-HSA-6798695. Neutrophil degranulation.
R-HSA-68827. CDT1 association with the CDC6:ORC:origin complex.
R-HSA-68949. Orc1 removal from chromatin.
R-HSA-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-HSA-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-HSA-69481. G2/M Checkpoints.
R-HSA-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-HSA-8852276. The role of GTSE1 in G2/M progression after G2 checkpoint.
R-HSA-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

ChiTaRSiPSMC3. human.
GeneWikiiPSMC3.
GenomeRNAii5702.
PMAP-CutDBP17980.
PROiP17980.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000165916.
CleanExiHS_PSMC3.
ExpressionAtlasiP17980. baseline and differential.
GenevisibleiP17980. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR005937. 26S_Psome_P45.
IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01242. 26Sp45. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRS6A_HUMAN
AccessioniPrimary (citable) accession number: P17980
Secondary accession number(s): B2R8V1
, Q3B757, Q3B865, Q53HU5, Q6GPG8, Q6IBS1, Q96HD3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: May 10, 2002
Last modified: November 30, 2016
This is version 185 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.