P17974 (GUN_RALSL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase
EC=3.2.1.4

Alternative name(s):
Cellulase
Endo-1,4-beta-glucanase

Gene names

Name:

egl

Organism

Ralstonia solanacearum (Pseudomonas solanacearum)

Taxonomic identifier

305 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Cell membrane; Lipid-anchor Probable.
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Cellular component	Cell membrane Membrane
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Lipoprotein Palmitate Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19
	Propeptide	20 – 45	26			PRO_0000007869
	Chain	46 – 426	381	Endoglucanase		PRO_0000007870
Sites
	Active site	249	1	Proton donor By similarity
	Active site	361	1	Nucleophile By similarity
Amino acid modifications
	Lipidation	20	1	N-palmitoyl cysteine
	Lipidation	20	1	S-diacylglycerol cysteine

Sequences

	Sequence		Length	Mass (Da)
	P17974 [UniParc]. Last modified May 1, 1992. Version 2. Checksum: 51E13AD4442CF4A8	FASTA	426	45,578
	10 20 30 40 50 60 MRRCMPLVAA SVAALMLAGC GGGDGDPSLS TASVSATDTT TLKPAATSTT SSVWLTLAKD 70 80 90 100 110 120 SAAFTVSGTR TVRYGAGSAW VEKSVSGSGR CTSTFFGKDP AAGVAKVCQL LQGTGTLLWR 130 140 150 160 170 180 GVSLAGAEFG EGSLPGTYGS NYIYPSADSV TYYKNKGMNL VRLPFRWERL QPTLNQVFDA 190 200 210 220 230 240 NELSRLTGFV NAVTATGQTV LLDPHNYARY YGNVIGSSAV PNSAYADFWR RLATQFKSNP 250 260 270 280 290 300 RVILGLMNEP NSMPTEQWLS GANAELAAIR SANASNVVFV PGNAWTGAHS WNQNWYGTPN 310 320 330 340 350 360 GTVMKGINDP GHNLVFEVHQ YLDGDSSGQS ANCVSATIGA QRLQDFTTWL RSNGYRGFLG 370 380 390 400 410 420 EFGAASNDTC NQAVSNMLTF VKNNADVWTG WAWWAGGPWW GGYMYSIEPS NGVDKPQMSV LAPYLK

References

[1]	"Role of the two-component leader sequence and mature amino acid sequences in extracellular export of endoglucanase EGL from Pseudomonas solanacearum." Huang J., Schell M.A. J. Bacteriol. 174:1314-1323(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: AW.
[2]	"Excretion of the egl gene product of Pseudomonas solanacearum." Huang J., Sukordhaman M., Schell M.A. J. Bacteriol. 171:3767-3774(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-112, PARTIAL PROTEIN SEQUENCE.
[3]	"Evidence that extracellular export of the endoglucanase encoded by egl of Pseudomonas solanacearum occurs by a two-step process involving a lipoprotein intermediate." Huang J., Schell M.A. J. Biol. Chem. 265:11628-11632(1990) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEOLYTIC PROCESSING.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M84922 Genomic DNA. Translation: AAA61980.1.
PIR	A42649.
3D structure databases
ProteinModelPortal	P17974.
ModBase	Search...
Protein family/group databases
CAZy	GH5. Glycoside Hydrolase Family 5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR001547. Glyco_hydro_5. IPR018087. Glyco_hydro_5_CS. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00150. Cellulase. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00659. GLYCOSYL_HYDROL_F5. 1 hit. PS51257. PROKAR_LIPOPROTEIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN_RALSL

Accession

Primary (citable) accession number: P17974

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	May 1, 1992
Last modified:	May 1, 2013
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program