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P17974

- GUN_RALSL

UniProt

P17974 - GUN_RALSL

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Protein

Endoglucanase

Gene

egl

Organism
Ralstonia solanacearum (Pseudomonas solanacearum)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei249 – 2491Proton donorBy similarity
Active sitei361 – 3611NucleophileBy similarity

GO - Molecular functioni

  1. cellulase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiGH5. Glycoside Hydrolase Family 5.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase (EC:3.2.1.4)
Alternative name(s):
Cellulase
Endo-1,4-beta-glucanase
Gene namesi
Name:egl
OrganismiRalstonia solanacearum (Pseudomonas solanacearum)
Taxonomic identifieri305 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia

Subcellular locationi

Cell membrane Curated; Lipid-anchor Curated

GO - Cellular componenti

  1. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Add
BLAST
Propeptidei20 – 4526PRO_0000007869Add
BLAST
Chaini46 – 426381EndoglucanasePRO_0000007870Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi20 – 201N-palmitoyl cysteine1 PublicationPROSITE-ProRule annotation
Lipidationi20 – 201S-diacylglycerol cysteine1 PublicationPROSITE-ProRule annotation

Keywords - PTMi

Lipoprotein, Palmitate, Zymogen

Structurei

3D structure databases

ProteinModelPortaliP17974.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00659. GLYCOSYL_HYDROL_F5. 1 hit.
PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17974 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRRCMPLVAA SVAALMLAGC GGGDGDPSLS TASVSATDTT TLKPAATSTT
60 70 80 90 100
SSVWLTLAKD SAAFTVSGTR TVRYGAGSAW VEKSVSGSGR CTSTFFGKDP
110 120 130 140 150
AAGVAKVCQL LQGTGTLLWR GVSLAGAEFG EGSLPGTYGS NYIYPSADSV
160 170 180 190 200
TYYKNKGMNL VRLPFRWERL QPTLNQVFDA NELSRLTGFV NAVTATGQTV
210 220 230 240 250
LLDPHNYARY YGNVIGSSAV PNSAYADFWR RLATQFKSNP RVILGLMNEP
260 270 280 290 300
NSMPTEQWLS GANAELAAIR SANASNVVFV PGNAWTGAHS WNQNWYGTPN
310 320 330 340 350
GTVMKGINDP GHNLVFEVHQ YLDGDSSGQS ANCVSATIGA QRLQDFTTWL
360 370 380 390 400
RSNGYRGFLG EFGAASNDTC NQAVSNMLTF VKNNADVWTG WAWWAGGPWW
410 420
GGYMYSIEPS NGVDKPQMSV LAPYLK
Length:426
Mass (Da):45,578
Last modified:May 1, 1992 - v2
Checksum:i51E13AD4442CF4A8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M84922 Genomic DNA. Translation: AAA61980.1.
PIRiA42649.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M84922 Genomic DNA. Translation: AAA61980.1 .
PIRi A42649.

3D structure databases

ProteinModelPortali P17974.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF00150. Cellulase. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Role of the two-component leader sequence and mature amino acid sequences in extracellular export of endoglucanase EGL from Pseudomonas solanacearum."
    Huang J., Schell M.A.
    J. Bacteriol. 174:1314-1323(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: AW.
  2. "Excretion of the egl gene product of Pseudomonas solanacearum."
    Huang J., Sukordhaman M., Schell M.A.
    J. Bacteriol. 171:3767-3774(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-112, PARTIAL PROTEIN SEQUENCE.
  3. "Evidence that extracellular export of the endoglucanase encoded by egl of Pseudomonas solanacearum occurs by a two-step process involving a lipoprotein intermediate."
    Huang J., Schell M.A.
    J. Biol. Chem. 265:11628-11632(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEOLYTIC PROCESSING, DIACYLGLYCEROL AT CYS-20, PALMITOYLATION AT CYS-20.

Entry informationi

Entry nameiGUN_RALSL
AccessioniPrimary (citable) accession number: P17974
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: May 1, 1992
Last modified: October 29, 2014
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3