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Protein

Potassium voltage-gated channel protein Shab

Gene

Shab

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.

GO - Molecular functioni

GO - Biological processi

  • action potential Source: FlyBase
  • chemical synaptic transmission Source: FlyBase
  • larval locomotory behavior Source: FlyBase
  • neuron projection morphogenesis Source: FlyBase
  • potassium ion transport Source: FlyBase
  • protein homooligomerization Source: InterPro
  • regulation of heart contraction Source: FlyBase
  • regulation of synaptic activity Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

ReactomeiR-DME-1296072. Voltage gated Potassium channels.
R-DME-381676. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.

Protein family/group databases

TCDBi1.A.1.2.1. the voltage-gated ion channel (vic) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium voltage-gated channel protein Shab
Gene namesi
Name:Shab
ORF Names:CG43128
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0262593. Shab.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei436 – 45419Helical; Name=Segment S1Add
BLAST
Transmembranei474 – 49522Helical; Name=Segment S2Add
BLAST
Transmembranei506 – 52722Helical; Name=Segment S3Add
BLAST
Transmembranei536 – 56126Helical; Name=Segment S4Add
BLAST
Transmembranei577 – 59822Helical; Name=Segment S5Add
BLAST
Transmembranei638 – 65922Helical; Name=Segment S6Add
BLAST

GO - Cellular componenti

  • integral component of membrane Source: FlyBase
  • plasma membrane Source: FlyBase
  • voltage-gated potassium channel complex Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi435 – 4351R → Q in allele Shab-1; temperature-sensitive paralytic. 1 Publication
Mutagenesisi608 – 6081V → D in allele Shab-1; temperature-sensitive paralytic. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 985985Potassium voltage-gated channel protein ShabPRO_0000053965Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi245 – 2451N-linked (GlcNAc...)Sequence analysis
Glycosylationi429 – 4291N-linked (GlcNAc...)Sequence analysis
Glycosylationi530 – 5301N-linked (GlcNAc...)Sequence analysis
Modified residuei690 – 6901Phosphoserine; by PKASequence analysis
Modified residuei731 – 7311Phosphoserine; by PKASequence analysis
Glycosylationi749 – 7491N-linked (GlcNAc...)Sequence analysis
Glycosylationi756 – 7561N-linked (GlcNAc...)Sequence analysis
Modified residuei796 – 7961Phosphoserine; by PKASequence analysis
Glycosylationi885 – 8851N-linked (GlcNAc...)Sequence analysis
Glycosylationi888 – 8881N-linked (GlcNAc...)Sequence analysis
Glycosylationi914 – 9141N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP17970.

Expressioni

Developmental stagei

Expressed in late embryos and pupae.

Gene expression databases

BgeeiFBgn0262593.
ExpressionAtlasiP17970. differential.
GenevisibleiP17970. DM.

Interactioni

Subunit structurei

Heterotetramer of potassium channel proteins.By similarity

Protein-protein interaction databases

BioGridi63854. 2 interactions.
STRINGi7227.FBpp0306030.

Structurei

3D structure databases

ProteinModelPortaliP17970.
SMRiP17970. Positions 276-669.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi623 – 6286Selectivity filterBy similarity

Domaini

The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3713. Eukaryota.
COG1226. LUCA.
InParanoidiP17970.
KOiK04885.
OrthoDBiEOG091G0FP3.
PhylomeDBiP17970.

Family and domain databases

InterProiIPR000210. BTB/POZ_dom.
IPR005821. Ion_trans_dom.
IPR003968. K_chnl_volt-dep_Kv.
IPR003971. K_chnl_volt-dep_Kv9.
IPR011333. SKP1/BTB/POZ.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view]
PANTHERiPTHR11537. PTHR11537. 2 hits.
PfamiPF02214. BTB_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR00169. KCHANNEL.
PR01494. KV9CHANNEL.
PR01491. KVCHANNEL.
SMARTiSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform B (identifier: P17970-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVGQLQGGQA AGQQQQQQQA TQQQQHSKQQ QQQQQQQQQQ LQLKQHQQQQ
60 70 80 90 100
QDILYQQHNE AIAIARGLQA ATPADIGDNQ PYYDTSGNVD WERAMGAGGA
110 120 130 140 150
GAYGGIGIGS LPAAGGAAYH LGPANPAGLV SRHLDYGDGG HLAGPSAGLP
160 170 180 190 200
AGAVGSGAGA GAGAGASVTG SGSGAGTGTG TGAGSGSGSG AAGKEVRYAP
210 220 230 240 250
FPVASPTHSI PTTSQQIVGS VGGVGVGGAS SQSISGGVPT HSQSNTTGAL
260 270 280 290 300
QRTHSRSMSS IPPPEPFMIA QSKAVNSRVS INVGGVRHEV LWRTLERLPH
310 320 330 340 350
TRLGRLRECT THEAIVELCD DYSLADNEYF FDRHPKSFSS ILNFYRTGKL
360 370 380 390 400
HIVDEMCVLA FSDDLEYWGV DELYLESCCQ HKYHQRKENV HEEMRKEAES
410 420 430 440 450
LRQRDEEEFG EGKFSEYQKY LWELLEKPNT SFAARVIAVI SILFIVLSTI
460 470 480 490 500
ALTLNTLPQL QHIDNGTPQD NPQLAMVEAV CITWFTLEYI LRFSASPDKW
510 520 530 540 550
KFFKGGLNII DLLAILPYFV SLFLLETNKN ATDQFQDVRR VVQVFRIMRI
560 570 580 590 600
LRVLKLARHS TGLQSLGFTL RNSYKELGLL MLFLAMGVLI FSSLAYFAEK
610 620 630 640 650
DEKDTKFVSI PEAFWWAGIT MTTVGYGDIC PTTALGKVIG TVCCICGVLV
660 670 680 690 700
VALPIPIIVN NFAEFYKNQM RREKALKRRE ALDRAKREGS IVSFHHINLK
710 720 730 740 750
DAFAKSMDLI DVIVDTGKQT NVVHPKGKRQ STPNIGRQTL DVQSAPGHNL
760 770 780 790 800
SQTDGNSTEG ESTSGRNPAT TGTGCYKNYD HVANLRNSNL HNRRGSSSEQ
810 820 830 840 850
DAVPPYSFDN PNARQTSMMA MESYRREQQA LLQQQQQQQQ QMLQMQQIQQ
860 870 880 890 900
KAPNGNGGAT GGGVANNLAM VAASSAATAV ATATNASNAS NTAPGSEGAE
910 920 930 940 950
GGGDGDGGGV DDDNLSQAKG LPIQMMITPG EVAELRRQVA LENLQNQRMD
960 970 980
NLEQDVPVEF ECCFCTTKGL PGCHGECIPL RANSV
Length:985
Mass (Da):106,359
Last modified:November 2, 2001 - v2
Checksum:i59E38AD35F064AC8
GO
Isoform A (identifier: P17970-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     717-746: Missing.

Show »
Length:955
Mass (Da):103,135
Checksum:iBAEFBFADC1AEAA6D
GO

Sequence cautioni

The sequence AAG22232 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAG22233 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti31 – 311Q → L (PubMed:2493160).Curated
Sequence conflicti31 – 311Q → L (PubMed:2333511).Curated
Sequence conflicti31 – 311Q → L (PubMed:2336395).Curated
Sequence conflicti220 – 2201S → G (PubMed:2493160).Curated
Sequence conflicti220 – 2201S → G (PubMed:2333511).Curated
Sequence conflicti220 – 2201S → G (PubMed:2336395).Curated
Sequence conflicti307 – 3071R → G (PubMed:2493160).Curated
Sequence conflicti307 – 3071R → G (PubMed:2333511).Curated
Sequence conflicti307 – 3071R → G (PubMed:2336395).Curated
Sequence conflicti362 – 3621S → G (PubMed:2493160).Curated
Sequence conflicti362 – 3621S → G (PubMed:2333511).Curated
Sequence conflicti362 – 3621S → G (PubMed:2336395).Curated
Sequence conflicti414 – 4152FS → CA in AAG22232 (PubMed:10731132).Curated
Sequence conflicti414 – 4152FS → CA in AAG22233 (PubMed:10731132).Curated
Sequence conflicti495 – 4951A → S (PubMed:2493160).Curated
Sequence conflicti495 – 4951A → S (PubMed:2333511).Curated
Sequence conflicti495 – 4951A → S (PubMed:2336395).Curated
Sequence conflicti553 – 5531V → I in AAG22232 (PubMed:10731132).Curated
Sequence conflicti553 – 5531V → I in AAG22233 (PubMed:10731132).Curated
Sequence conflicti613 – 6131A → T in AAG22232 (PubMed:10731132).Curated
Sequence conflicti613 – 6131A → T in AAG22233 (PubMed:10731132).Curated
Sequence conflicti630 – 6301C → Y in AAG22232 (PubMed:10731132).Curated
Sequence conflicti630 – 6301C → Y in AAG22233 (PubMed:10731132).Curated
Sequence conflicti651 – 6511V → I in AAG22232 (PubMed:10731132).Curated
Sequence conflicti651 – 6511V → I in AAG22233 (PubMed:10731132).Curated
Sequence conflicti827 – 8282EQ → DE (PubMed:2493160).Curated
Sequence conflicti827 – 8282EQ → DE (PubMed:2333511).Curated
Sequence conflicti827 – 8282EQ → DE (PubMed:2336395).Curated
Sequence conflicti877 – 8771A → G (PubMed:2493160).Curated
Sequence conflicti877 – 8771A → G (PubMed:2333511).Curated
Sequence conflicti877 – 8771A → G (PubMed:2336395).Curated
Sequence conflicti903 – 98583GDGDG…RANSV → VMEMGAVSMTTTFPRPRDCP SR (PubMed:2493160).CuratedAdd
BLAST
Sequence conflicti903 – 98583GDGDG…RANSV → VMEMGAVSMTTTFPRPRDCP SR (PubMed:2333511).CuratedAdd
BLAST
Sequence conflicti903 – 98583GDGDG…RANSV → VMEMGAVSMTTTFPRPRDCP SR (PubMed:2336395).CuratedAdd
BLAST
Sequence conflicti911 – 9133DDD → NDN in AAC33365 (PubMed:10419540).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei717 – 74630Missing in isoform A. 1 PublicationVSP_000960Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32659 mRNA. Translation: AAA28896.1.
AF084525 mRNA. Translation: AAC33365.1.
AE014296 Genomic DNA. Translation: AAG22232.2. Different initiation.
AE014296 Genomic DNA. Translation: AAG22233.1. Different initiation.
PIRiS12746.
RefSeqiNP_523894.2. NM_079170.6.
NP_728783.1. NM_167967.4.
UniGeneiDm.2563.

Genome annotation databases

GeneIDi38352.
KEGGidme:Dmel_CG43128.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32659 mRNA. Translation: AAA28896.1.
AF084525 mRNA. Translation: AAC33365.1.
AE014296 Genomic DNA. Translation: AAG22232.2. Different initiation.
AE014296 Genomic DNA. Translation: AAG22233.1. Different initiation.
PIRiS12746.
RefSeqiNP_523894.2. NM_079170.6.
NP_728783.1. NM_167967.4.
UniGeneiDm.2563.

3D structure databases

ProteinModelPortaliP17970.
SMRiP17970. Positions 276-669.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi63854. 2 interactions.
STRINGi7227.FBpp0306030.

Protein family/group databases

TCDBi1.A.1.2.1. the voltage-gated ion channel (vic) superfamily.

Proteomic databases

PaxDbiP17970.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi38352.
KEGGidme:Dmel_CG43128.

Organism-specific databases

CTDi38352.
FlyBaseiFBgn0262593. Shab.

Phylogenomic databases

eggNOGiKOG3713. Eukaryota.
COG1226. LUCA.
InParanoidiP17970.
KOiK04885.
OrthoDBiEOG091G0FP3.
PhylomeDBiP17970.

Enzyme and pathway databases

ReactomeiR-DME-1296072. Voltage gated Potassium channels.
R-DME-381676. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.

Miscellaneous databases

ChiTaRSiShab. fly.
GenomeRNAii38352.
PROiP17970.

Gene expression databases

BgeeiFBgn0262593.
ExpressionAtlasiP17970. differential.
GenevisibleiP17970. DM.

Family and domain databases

InterProiIPR000210. BTB/POZ_dom.
IPR005821. Ion_trans_dom.
IPR003968. K_chnl_volt-dep_Kv.
IPR003971. K_chnl_volt-dep_Kv9.
IPR011333. SKP1/BTB/POZ.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view]
PANTHERiPTHR11537. PTHR11537. 2 hits.
PfamiPF02214. BTB_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR00169. KCHANNEL.
PR01494. KV9CHANNEL.
PR01491. KVCHANNEL.
SMARTiSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKCNAB_DROME
AccessioniPrimary (citable) accession number: P17970
Secondary accession number(s): O76805, Q9I7T9, Q9I7U0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 2, 2001
Last modified: September 7, 2016
This is version 150 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Miscellaneous

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.