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P17968

- VP7_ROTB6

UniProt

P17968 - VP7_ROTB6

Protein

Outer capsid glycoprotein VP7

Gene
N/A
Organism
Rotavirus A (isolate Cow/Thailand/61A/1988 G10-P7[5]-Ix-Rx-Cx-Mx-Ax-Nx-Tx-Ex-Hx) (RV-A)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (01 Nov 1990)
      Previous versions | rss
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    Functioni

    Outer capsid protein involved in attachment and possibly entry into the host epithelial cell. It is subsequently lost, together with VP4, following virus entry into the host cell. The outer layer contains 780 copies of VP7, grouped as 260 trimers. Rotavirus attachment and entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. In integrin-dependent strains, VP7 seems to essentially target the integrin heterodimers ITGAX/ITGB2 and ITGA5/ITGB3 at a postbinding stage, once the initial attachment by VP4 has been achieved By similarity.By similarity

    GO - Biological processi

    1. viral process Source: UniProtKB-KW

    Keywords - Biological processi

    Host-virus interaction

    Keywords - Ligandi

    Calcium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Outer capsid glycoprotein VP7
    OrganismiRotavirus A (isolate Cow/Thailand/61A/1988 G10-P7[5]-Ix-Rx-Cx-Mx-Ax-Nx-Tx-Ex-Hx) (RV-A)
    Taxonomic identifieri10929 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
    Virus hostiBos taurus (Bovine) [TaxID: 9913]

    Subcellular locationi

    Virion By similarity. Host endoplasmic reticulum lumen Curated
    Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles By similarity.By similarity

    GO - Cellular componenti

    1. host cell endoplasmic reticulum lumen Source: UniProtKB-SubCell
    2. T=13 icosahedral viral capsid Source: UniProtKB-KW
    3. viral outer capsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid protein, Host endoplasmic reticulum, Outer capsid protein, T=13 icosahedral capsid protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 5050Sequence AnalysisAdd
    BLAST
    Chaini51 – 326276Outer capsid glycoprotein VP7Sequence AnalysisPRO_0000149580Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi69 – 691N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi145 – 1451N-linked (GlcNAc...); by hostSequence Analysis

    Post-translational modificationi

    The N-terminus is blocked possibly by pyroglutamic acid.By similarity
    N-glycosylated.By similarity
    Intramolecular disulfide bonds.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Subunit structurei

    Homotrimer; in the presence of calcium By similarity. Acquisition of the capsid outer layer requires a high calcium concentration inside the endoplasmic reticulum. Following cell entry, the low calcium concentration in the cytoplasm is probably responsible for the solubilization of the outer layer. Interacts with host integrin heterodimers ITGAX/ITGB2 and ITGA5/ITGB3 By similarity.By similarity

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the rotavirus VP7 family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    InterProiIPR001963. VP7.
    [Graphical view]
    PfamiPF00434. VP7. 1 hit.
    [Graphical view]
    ProDomiPD000191. VP7. 1 hit.
    [Graphical view] [Entries sharing at least one domain]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative initiation. Align

    Isoform 1 (identifier: P17968-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MYGIEYTTFL IYLISIILFN YILKSITRMM DYIIYKFLLI VTIASIVVNA    50
    QNYGINLPIT GSMDASYVNA TKDKPFLTST LCLYYPTEAR TEINDNEWTS 100
    TLSQLFLTKG WPTGSVYFKE YDDIATFSVD PQLYCDYNIV LMRYNSSLEL 150
    DMSELANLIL NEWLCNPMDI TLYYYQQNGQ ANKWIAMGQS CTIKVCPLNT 200
    QTLGIGCQTT NTRTFEEVAT AEKLVITDVV DGVNHKLDVT TATCTIRNCK 250
    KLGPRENVAV IQVGGADILD ITSDPTTAPQ TERMMRINWK KWWQVFYTIV 300
    DYVNQIVQAM SKRSRSLNSA AFYYRV 326
    Length:326
    Mass (Da):37,310
    Last modified:November 1, 1990 - v1
    Checksum:iDF486855D90442D0
    GO
    Isoform 2 (identifier: P17968-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-29: Missing.

    Note: Produced by alternative initiation at Met-30 of isoform 1. No experimental confirmation available.

    Show »
    Length:297
    Mass (Da):33,794
    Checksum:i13638B091036D5EA
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 2929Missing in isoform 2. CuratedVSP_038583Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53403 Genomic RNA. Translation: CAA37478.1.

    Keywords - Coding sequence diversityi

    Alternative initiation

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53403 Genomic RNA. Translation: CAA37478.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR001963. VP7.
    [Graphical view ]
    Pfami PF00434. VP7. 1 hit.
    [Graphical view ]
    ProDomi PD000191. VP7. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the VP7 gene of a bovine rotavirus (strain 61A) with different serotype specificity from serotype 6."
      Taniguchi K., Pongsuwanna Y., Choonthanom M., Urasawa S.
      Nucleic Acids Res. 18:4613-4613(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiVP7_ROTB6
    AccessioniPrimary (citable) accession number: P17968
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: November 1, 1990
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In group A rotaviruses, VP7 defines the G serotype.

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3