P17957 (CHS2_SOYBN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chalcone synthase 2
EC=2.3.1.74

Alternative name(s):
Naringenin-chalcone synthase 2

Gene names

Name:

CHS2

Organism

Glycine max (Soybean) (Glycine hispida) [Reference proteome]

Taxonomic identifier

3847 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Glycine

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.
Catalytic activity	3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.
Pathway	Secondary metabolite biosynthesis; flavonoid biosynthesis.
Sequence similarities	Belongs to the chalcone/stilbene synthases family.

Ontologies

Keywords
Biological process	Flavonoid biosynthesis
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	flavonoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	naringenin-chalcone synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 388	388	Chalcone synthase 2		PRO_0000216063
Sites
	Active site	164	1	By similarity
Experimental info
	Sequence conflict	9	1	K → Q in CAA46590. Ref.2
	Sequence conflict	100	1	M → V in CAA46590. Ref.2
	Sequence conflict	299	1	R → W in CAA46590. Ref.2
	Sequence conflict	387	1	T → S in CAA46590. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P17957 [UniParc]. Last modified November 1, 1990. Version 1. Checksum: 634D3F1CEDC5F973	FASTA	388	42,504
	10 20 30 40 50 60 MVSVEEIRKA QRAEGPATVM AIGTATPPNC VDQSTYPDYY FRITNSEHMT ELKEKFKRMC 70 80 90 100 110 120 DKSMIKKRYM YLNEEILKEN PSVCAYMAPS LDARQDMVVM EVPKLGKEAA TKAIKEWGQP 130 140 150 160 170 180 KSKITHLIFC TTSGVDMPGA DYQLTKLLGL RPSVKRYMMY QQGCFAGGTV LRLAKDLAEN 190 200 210 220 230 240 NKGARVLVVC SEITAVTFRG PTDTHLDSLV GQALFGDGAA AVIVGSDPLP VEKPLFQLVW 250 260 270 280 290 300 TAQTILPDSE GAIDGHLREV GLTFHLLKDV PGLISKNIEK ALVEAFQPLG ISDYNSIFRI 310 320 330 340 350 360 AHPGGPAILD QVEAKLGLKP EKMEATRHVL SEYGNMSSAC VLFILDQMRK KSIENGLGTT 370 380 GEGLDWGVLF GFGPGLTVET VVLRSVTV

References

[1]	"Nucleotide sequence of one member of soybean chalcone synthase multi-gene family." Akada S., Kung S.D., Dube S.K. Nucleic Acids Res. 18:3398-3398(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Williams.
[2]	"Nucleotide sequence and putative regulatory elements of gene 2 of the soybean (Glycine max) chalcone synthase multigene family." Akada S., Kung S.D., Dube S.K. Plant Physiol. 102:317-319(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Williams.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X52097 Genomic DNA. Translation: CAA36317.1. X65636 Genomic DNA. Translation: CAA46590.1.
PIR	JQ2249. SYSYCN. S16338.
RefSeq	XP_003524935.1. XM_003524887.1.
3D structure databases
ProteinModelPortal	P17957.
SMR	P17957. Positions 3-388.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	GLYMA05G28610.1; GLYMA05G28610.1; GLYMA05G28610. GLYMA05G28610.2; GLYMA05G28610.2; GLYMA05G28610.
GeneID	100775307.
KEGG	gmx:100775307.
Enzyme and pathway databases
UniPathway	UPA00154.
Gene expression databases
Genevestigator	P17957.
Family and domain databases
Gene3D	3.40.47.10. 2 hits.
InterPro	IPR012328. Chalcone/stilbene_synth_C. IPR018088. Chalcone/stilbene_synthase_AS. IPR001099. Chalcone/stilbene_synthase_N. IPR011141. Polyketide_synthase_type-III. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Pfam	PF02797. Chal_sti_synt_C. 1 hit. PF00195. Chal_sti_synt_N. 1 hit. [Graphical view]
PIRSF	PIRSF000451. PKS_III. 1 hit.
SUPFAM	SSF53901. Thiolase-like. 2 hits.
PROSITE	PS00441. CHALCONE_SYNTH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHS2_SOYBN

Accession

Primary (citable) accession number: P17957

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	April 3, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program