Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot P17952 (MURC_ECOLI)

Last modified November 3, 2009. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    UDP-N-acetylmuramate--L-alanine ligase
    EC=6.3.2.8
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanine synthetase
Gene names
Name: murC
Ordered Locus Names: b0091, JW0089
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Hide | Top

Protein attributes

Sequence length491 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

Hide | Top

General annotation (Comments)

Function

Cell wall formation. HAMAP MF_00046

Catalytic activity

ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046

Subcellular location

Cytoplasm Probable.

Sequence similarities

Belongs to the murCDEF family.

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 491491UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046
PRO_0000182089

Regions

Nucleotide binding126 – 1327ATP Potential

Experimental info

Mutagenesis3441G → D in murC3.

Secondary structure

1 ...................................................................................... 491
Helix Strand Turn

Details...

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
P17952-1 [UniParc].

Last modified August 1, 1991. Version 2.
Checksum: D201B35931C013FB

FASTA49153,626
        10         20         30         40         50         60 
MNTQQLAKLR SIVPEMRRVR HIHFVGIGGA GMGGIAEVLA NEGYQISGSD LAPNPVTQQL 

        70         80         90        100        110        120 
MNLGATIYFN HRPENVRDAS VVVVSSAISA DNPEIVAAHE ARIPVIRRAE MLAELMRFRH 

       130        140        150        160        170        180 
GIAIAGTHGK TTTTAMVSSI YAEAGLDPTF VNGGLVKAAG VHARLGHGRY LIAEADESDA 

       190        200        210        220        230        240 
SFLHLQPMVA IVTNIEADHM DTYQGDFENL KQTFINFLHN LPFYGRAVMC VDDPVIRELL 

       250        260        270        280        290        300 
PRVGRQTTTY GFSEDADVRV EDYQQIGPQG HFTLLRQDKE PMRVTLNAPG RHNALNAAAA 

       310        320        330        340        350        360 
VAVATEEGID DEAILRALES FQGTGRRFDF LGEFPLEPVN GKSGTAMLVD DYGHHPTEVD 

       370        380        390        400        410        420 
ATIKAARAGW PDKNLVMLFQ PHRFTRTRDL YDDFANVLTQ VDTLLMLEVY PAGEAPIPGA 

       430        440        450        460        470        480 
DSRSLCRTIR GRGKIDPILV PDPARVAEML APVLTGNDLI LVQGAGNIGK IARSLAEIKL 

       490 
KPQTPEEEQH D

« Hide

Hide | Top

References

« Hide 'large scale' references
[1]"Nucleotide sequence involving murG and murC in the mra gene cluster region of Escherichia coli."
Ikeda M., Wachi M., Jung H.K., Ishino F., Matsuhashi M.
Nucleic Acids Res. 18:4014-4014(1990) [PubMed: 2197603] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region."
Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A.
Nucleic Acids Res. 20:3305-3308(1992) [PubMed: 1630901] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Conditionally lethal Escherichia coli murein mutants contain point defects that map to regions conserved among murein and folyl poly-gamma-glutamate ligases: identification of a ligase superfamily."
Eveland S.S., Pompliano D.L., Anderson M.S.
Biochemistry 36:6223-6229(1997) [PubMed: 9166795] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (MUTANT MURC3).
Strain: CGSC 5988.
[6]"Over-production, purification and properties of the uridine-diphosphate-N-acetylmuramate:L-alanine ligase from Escherichia coli."
Liger D., Masson A., Blanot D., van Heijenoort J., Parquet C.
Eur. J. Biochem. 230:80-87(1995) [PubMed: 7601127] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-14, CHARACTERIZATION.
+Additional computationally mapped references.

Hide | Top

Cross-references

Sequence databases

X52644 Genomic DNA. Translation: CAA36868.1.
X55034 Genomic DNA. Translation: CAA38868.1.
U00096 Genomic DNA. Translation: AAC73202.1.
AP009048 Genomic DNA. Translation: BAB96659.1.
U67892 Genomic DNA. Translation: AAB60787.1.
PIRCEECAM. JQ0545.
RefSeqAP_000754.1.
NP_414633.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1CC9model-A1-491[»]
2F00X-ray2.50A/B1-491[»]
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:10278N.
STRINGP17952.

Genome annotation databases

GeneID946153.
GenomeReviewsGene locus JW0089 in contig AP009048_GR.
Gene locus b0091 in contig U00096_GR.
KEGGecj:JW0089.
eco:b0091.

Organism-specific databases

EchoBASEEB0614.
EcoGeneEG10619. murC.
CMRSearch...

Phylogenomic databases

HOGENOMP17952.
OMAEWMVVEA.

Enzyme and pathway databases

BioCycEcoCyc:UDP-NACMUR-ALA-LIG-MON.
MetaCyc:UDP-NACMUR-ALA-LIG-MON.

Gene expression databases

GenevestigatorP17952.

Family and domain databases

HAMAPMF_00046.
[Tree]
InterProIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR016040. NAD(P)-bd_dom.
IPR005758. UDP-N-AcMur_Ala_ligase.
[Graphical view]
Gene3DG3DSA:3.90.190.20. Mur_ligase_C. 1 hit.
G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PfamPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
TIGRFAMsTIGR01082. murC. 1 hit.
ProtoNetSearch...

Hide | Top

Entry information

Entry nameMURC_ECOLI
AccessionPrimary (citable) accession number: P17952
Secondary accession number(s): O07099
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: August 1, 1991
Last modified: November 3, 2009
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Hide | Top

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents