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Protein

UDP-N-acetylmuramate--L-alanine ligase

Gene

murC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cell wall formation.1 Publication

Catalytic activityi

ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine.1 Publication

Kineticsi

  1. KM=100 µM for UDP-N-acetyl-alpha-D-muramate1 Publication
  2. KM=20 µM for L-alanine1 Publication
  3. KM=450 µM for ATP1 Publication
  1. Vmax=17.3 µmol/min/mg enzyme1 Publication

pH dependencei

Optimum pH is 8.6.1 Publication

Temperature dependencei

Optimum temperature is 45 degrees Celsius.1 Publication

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi126 – 1327ATPSequence analysis

GO - Molecular functioni

  • ATP binding Source: EcoliWiki
  • magnesium ion binding Source: EcoliWiki
  • UDP-N-acetylmuramate-L-alanine ligase activity Source: EcoliWiki

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • cell wall organization Source: UniProtKB-KW
  • peptidoglycan biosynthetic process Source: EcoCyc
  • regulation of cell shape Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:UDP-NACMUR-ALA-LIG-MONOMER.
ECOL316407:JW0089-MONOMER.
MetaCyc:UDP-NACMUR-ALA-LIG-MONOMER.
BRENDAi6.3.2.8. 2026.
UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylmuramate--L-alanine ligase (EC:6.3.2.8)
Alternative name(s):
UDP-N-acetylmuramoyl-L-alanine synthetase
Gene namesi
Name:murC
Ordered Locus Names:b0091, JW0089
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10619. murC.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi344 – 3441G → D in murC3.

Chemistry

ChEMBLiCHEMBL5168.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 491491UDP-N-acetylmuramate--L-alanine ligasePRO_0000182089Add
BLAST

Proteomic databases

EPDiP17952.
PaxDbiP17952.
PRIDEiP17952.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
flxAP776093EBI-554607,EBI-553024

Protein-protein interaction databases

BioGridi4261857. 473 interactions.
DIPiDIP-10278N.
IntActiP17952. 15 interactions.
MINTiMINT-1222662.
STRINGi511145.b0091.

Chemistry

BindingDBiP17952.

Structurei

Secondary structure

1
491
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 97Combined sources
Turni10 – 123Combined sources
Beta strandi21 – 266Combined sources
Helixi32 – 4110Combined sources
Beta strandi45 – 495Combined sources
Helixi55 – 628Combined sources
Beta strandi66 – 705Combined sources
Helixi73 – 764Combined sources
Beta strandi80 – 845Combined sources
Helixi93 – 1008Combined sources
Beta strandi105 – 1073Combined sources
Helixi108 – 1169Combined sources
Beta strandi119 – 12810Combined sources
Helixi130 – 14314Combined sources
Beta strandi149 – 1513Combined sources
Turni157 – 1593Combined sources
Beta strandi160 – 1645Combined sources
Beta strandi167 – 1748Combined sources
Helixi181 – 1844Combined sources
Beta strandi188 – 1925Combined sources
Helixi200 – 2023Combined sources
Turni203 – 2053Combined sources
Helixi207 – 21812Combined sources
Beta strandi226 – 2305Combined sources
Helixi234 – 2396Combined sources
Helixi240 – 2423Combined sources
Beta strandi245 – 2539Combined sources
Beta strandi257 – 26610Combined sources
Beta strandi269 – 2757Combined sources
Beta strandi282 – 2887Combined sources
Helixi291 – 30717Combined sources
Helixi311 – 3199Combined sources
Beta strandi327 – 34115Combined sources
Beta strandi343 – 3519Combined sources
Helixi356 – 36712Combined sources
Beta strandi372 – 3798Combined sources
Helixi384 – 3874Combined sources
Turni388 – 3903Combined sources
Helixi391 – 3988Combined sources
Beta strandi401 – 4066Combined sources
Beta strandi412 – 4143Combined sources
Helixi422 – 4254Combined sources
Helixi447 – 4504Combined sources
Helixi451 – 4533Combined sources
Beta strandi456 – 4638Combined sources
Helixi468 – 47710Combined sources
Turni478 – 4803Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CC9model-A1-491[»]
2F00X-ray2.50A/B1-491[»]
ProteinModelPortaliP17952.
SMRiP17952. Positions 1-483.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP17952.

Family & Domainsi

Sequence similaritiesi

Belongs to the MurCDEF family.Curated

Phylogenomic databases

eggNOGiENOG4105DFU. Bacteria.
COG0773. LUCA.
HOGENOMiHOG000256031.
InParanoidiP17952.
KOiK01924.
OMAiFHFIGIG.
OrthoDBiEOG64BQ73.
PhylomeDBiP17952.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.40.50.720. 1 hit.
3.90.190.20. 1 hit.
HAMAPiMF_00046. MurC.
InterProiIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR016040. NAD(P)-bd_dom.
IPR005758. UDP-N-AcMur_Ala_ligase_murC.
[Graphical view]
PfamiPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
TIGRFAMsiTIGR01082. murC. 1 hit.

Sequencei

Sequence statusi: Complete.

P17952-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTQQLAKLR SIVPEMRRVR HIHFVGIGGA GMGGIAEVLA NEGYQISGSD
60 70 80 90 100
LAPNPVTQQL MNLGATIYFN HRPENVRDAS VVVVSSAISA DNPEIVAAHE
110 120 130 140 150
ARIPVIRRAE MLAELMRFRH GIAIAGTHGK TTTTAMVSSI YAEAGLDPTF
160 170 180 190 200
VNGGLVKAAG VHARLGHGRY LIAEADESDA SFLHLQPMVA IVTNIEADHM
210 220 230 240 250
DTYQGDFENL KQTFINFLHN LPFYGRAVMC VDDPVIRELL PRVGRQTTTY
260 270 280 290 300
GFSEDADVRV EDYQQIGPQG HFTLLRQDKE PMRVTLNAPG RHNALNAAAA
310 320 330 340 350
VAVATEEGID DEAILRALES FQGTGRRFDF LGEFPLEPVN GKSGTAMLVD
360 370 380 390 400
DYGHHPTEVD ATIKAARAGW PDKNLVMLFQ PHRFTRTRDL YDDFANVLTQ
410 420 430 440 450
VDTLLMLEVY PAGEAPIPGA DSRSLCRTIR GRGKIDPILV PDPARVAEML
460 470 480 490
APVLTGNDLI LVQGAGNIGK IARSLAEIKL KPQTPEEEQH D
Length:491
Mass (Da):53,626
Last modified:August 1, 1991 - v2
Checksum:iD201B35931C013FB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52644 Genomic DNA. Translation: CAA36868.1.
X55034 Genomic DNA. Translation: CAA38868.1.
U00096 Genomic DNA. Translation: AAC73202.1.
AP009048 Genomic DNA. Translation: BAB96659.1.
U67892 Genomic DNA. Translation: AAB60787.1.
PIRiJQ0545. CEECAM.
RefSeqiNP_414633.1. NC_000913.3.
WP_001096049.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73202; AAC73202; b0091.
BAB96659; BAB96659; BAB96659.
GeneIDi946153.
KEGGiecj:JW0089.
eco:b0091.
PATRICi32115287. VBIEscCol129921_0095.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52644 Genomic DNA. Translation: CAA36868.1.
X55034 Genomic DNA. Translation: CAA38868.1.
U00096 Genomic DNA. Translation: AAC73202.1.
AP009048 Genomic DNA. Translation: BAB96659.1.
U67892 Genomic DNA. Translation: AAB60787.1.
PIRiJQ0545. CEECAM.
RefSeqiNP_414633.1. NC_000913.3.
WP_001096049.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CC9model-A1-491[»]
2F00X-ray2.50A/B1-491[»]
ProteinModelPortaliP17952.
SMRiP17952. Positions 1-483.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261857. 473 interactions.
DIPiDIP-10278N.
IntActiP17952. 15 interactions.
MINTiMINT-1222662.
STRINGi511145.b0091.

Chemistry

BindingDBiP17952.
ChEMBLiCHEMBL5168.

Proteomic databases

EPDiP17952.
PaxDbiP17952.
PRIDEiP17952.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73202; AAC73202; b0091.
BAB96659; BAB96659; BAB96659.
GeneIDi946153.
KEGGiecj:JW0089.
eco:b0091.
PATRICi32115287. VBIEscCol129921_0095.

Organism-specific databases

EchoBASEiEB0614.
EcoGeneiEG10619. murC.

Phylogenomic databases

eggNOGiENOG4105DFU. Bacteria.
COG0773. LUCA.
HOGENOMiHOG000256031.
InParanoidiP17952.
KOiK01924.
OMAiFHFIGIG.
OrthoDBiEOG64BQ73.
PhylomeDBiP17952.

Enzyme and pathway databases

UniPathwayiUPA00219.
BioCyciEcoCyc:UDP-NACMUR-ALA-LIG-MONOMER.
ECOL316407:JW0089-MONOMER.
MetaCyc:UDP-NACMUR-ALA-LIG-MONOMER.
BRENDAi6.3.2.8. 2026.

Miscellaneous databases

EvolutionaryTraceiP17952.
PROiP17952.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.40.50.720. 1 hit.
3.90.190.20. 1 hit.
HAMAPiMF_00046. MurC.
InterProiIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR016040. NAD(P)-bd_dom.
IPR005758. UDP-N-AcMur_Ala_ligase_murC.
[Graphical view]
PfamiPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
TIGRFAMsiTIGR01082. murC. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence involving murG and murC in the mra gene cluster region of Escherichia coli."
    Ikeda M., Wachi M., Jung H.K., Ishino F., Matsuhashi M.
    Nucleic Acids Res. 18:4014-4014(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region."
    Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A.
    Nucleic Acids Res. 20:3305-3308(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Conditionally lethal Escherichia coli murein mutants contain point defects that map to regions conserved among murein and folyl poly-gamma-glutamate ligases: identification of a ligase superfamily."
    Eveland S.S., Pompliano D.L., Anderson M.S.
    Biochemistry 36:6223-6229(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (MUTANT MURC3).
    Strain: CGSC 5988.
  6. "Over-production, purification and properties of the uridine-diphosphate-N-acetylmuramate:L-alanine ligase from Escherichia coli."
    Liger D., Masson A., Blanot D., van Heijenoort J., Parquet C.
    Eur. J. Biochem. 230:80-87(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-14, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBSTRATE SPECIFICITY.

Entry informationi

Entry nameiMURC_ECOLI
AccessioniPrimary (citable) accession number: P17952
Secondary accession number(s): O07099
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: August 1, 1991
Last modified: April 13, 2016
This is version 161 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.