UDP-N-acetylmuramate--L-alanine ligase - Escherichia coli (strain K12)

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P17952 (MURC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 136. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramate--L-alanine ligase
EC=6.3.2.8

Alternative name(s):
UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	b0091, JW0089

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	491 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cell wall formation. HAMAP-Rule MF_00046
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP-Rule MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00046
Subcellular location	Cytoplasm Probable HAMAP-Rule MF_00046.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from direct assay PubMed 17139082. Source: EcoliWiki UDP-N-acetylmuramate-L-alanine ligase activity Inferred from direct assay Ref.6. Source: EcoliWiki magnesium ion binding Inferred from direct assay PubMed 17139082. Source: EcoliWiki
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 491

491

UDP-N-acetylmuramate--L-alanine ligase HAMAP-Rule MF_00046

PRO_0000182089

Regions

Nucleotide binding

126 – 132

ATP Potential

Experimental info

Mutagenesis

344

G → D in murC3.

Secondary structure

491

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P17952 [UniParc].

Last modified August 1, 1991. Version 2.
Checksum: D201B35931C013FB
Show »

FASTA

491

53,626

        10         20         30         40         50         60 
MNTQQLAKLR SIVPEMRRVR HIHFVGIGGA GMGGIAEVLA NEGYQISGSD LAPNPVTQQL 

        70         80         90        100        110        120 
MNLGATIYFN HRPENVRDAS VVVVSSAISA DNPEIVAAHE ARIPVIRRAE MLAELMRFRH 

       130        140        150        160        170        180 
GIAIAGTHGK TTTTAMVSSI YAEAGLDPTF VNGGLVKAAG VHARLGHGRY LIAEADESDA 

       190        200        210        220        230        240 
SFLHLQPMVA IVTNIEADHM DTYQGDFENL KQTFINFLHN LPFYGRAVMC VDDPVIRELL 

       250        260        270        280        290        300 
PRVGRQTTTY GFSEDADVRV EDYQQIGPQG HFTLLRQDKE PMRVTLNAPG RHNALNAAAA 

       310        320        330        340        350        360 
VAVATEEGID DEAILRALES FQGTGRRFDF LGEFPLEPVN GKSGTAMLVD DYGHHPTEVD 

       370        380        390        400        410        420 
ATIKAARAGW PDKNLVMLFQ PHRFTRTRDL YDDFANVLTQ VDTLLMLEVY PAGEAPIPGA 

       430        440        450        460        470        480 
DSRSLCRTIR GRGKIDPILV PDPARVAEML APVLTGNDLI LVQGAGNIGK IARSLAEIKL 

       490 
KPQTPEEEQH D

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence involving murG and murC in the mra gene cluster region of Escherichia coli." Ikeda M., Wachi M., Jung H.K., Ishino F., Matsuhashi M. Nucleic Acids Res. 18:4014-4014(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A. Nucleic Acids Res. 20:3305-3308(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Conditionally lethal Escherichia coli murein mutants contain point defects that map to regions conserved among murein and folyl poly-gamma-glutamate ligases: identification of a ligase superfamily." Eveland S.S., Pompliano D.L., Anderson M.S. Biochemistry 36:6223-6229(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (MUTANT MURC3). Strain: CGSC 5988.
[6]	"Over-production, purification and properties of the uridine-diphosphate-N-acetylmuramate:L-alanine ligase from Escherichia coli." Liger D., Masson A., Blanot D., van Heijenoort J., Parquet C. Eur. J. Biochem. 230:80-87(1995) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-14, CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X52644 Genomic DNA. Translation: CAA36868.1.
X55034 Genomic DNA. Translation: CAA38868.1.
U00096 Genomic DNA. Translation: AAC73202.1.
AP009048 Genomic DNA. Translation: BAB96659.1.
U67892 Genomic DNA. Translation: AAB60787.1.

PIR

CEECAM. JQ0545.

RefSeq

NP_414633.1. NC_000913.2.
YP_488396.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1CC9	model	-	A	1-491	[»]
2F00	X-ray	2.50	A/B	1-491	[»]

ProteinModelPortal

P17952.

SMR

P17952. Positions 1-483.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-10278N.

IntAct

P17952. 12 interactions.

MINT

MINT-1222662.

STRING

511145.b0091.

Proteomic databases

PaxDb

P17952.

PRIDE

P17952.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC73202; AAC73202; b0091.
BAB96659; BAB96659; BAB96659.

GeneID

12932863.
946153.

KEGG

ecj:Y75_p0090.
eco:b0091.

PATRIC

32115287. VBIEscCol129921_0095.

Organism-specific databases

EchoBASE

EB0614.

EcoGene

EG10619. murC.

Phylogenomic databases

eggNOG

COG0773.

HOGENOM

HOG000256031.

K01924.

OMA

YQPHRYS.

ProtClustDB

PRK00421.

Enzyme and pathway databases

BioCyc

EcoCyc:UDP-NACMUR-ALA-LIG-MONOMER.
ECOL316407:JW0089-MONOMER.
MetaCyc:UDP-NACMUR-ALA-LIG-MONOMER.

UniPathway

UPA00219.

Gene expression databases

Genevestigator

P17952.

Family and domain databases

Gene3D

3.40.1190.10. 1 hit.
3.40.50.720. 1 hit.
3.90.190.20. 1 hit.

HAMAP

MF_00046. MurC.

InterPro

IPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR016040. NAD(P)-bd_dom.
IPR005758. UDP-N-AcMur_Ala_ligase.
[Graphical view]

PANTHER

PTHR23135:SF5. PTHR23135:SF5. 1 hit.

Pfam

PF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]

SUPFAM

SSF53244. Mur_ligase_C. 1 hit.
SSF53623. Mur_ligase_cen. 1 hit.

TIGRFAMs

TIGR01082. murC. 1 hit.

ProtoNet

Search...

Other

BindingDB

P17952.

ChEMBL

CHEMBL5168.

EvolutionaryTrace

P17952.

Entry information

Entry name

MURC_ECOLI

Accession

Primary (citable) accession number: P17952
Secondary accession number(s): O07099

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	August 1, 1991
Last modified:	May 1, 2013
This is version 136 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents