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Protein

Transcription factor PU.1

Gene

SPI1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi170 – 253ETSPROSITE-ProRule annotationAdd BLAST84

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding, RNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-8939236 RUNX1 regulates transcription of genes involved in differentiation of HSCs
SignaLinkiP17947
SIGNORiP17947

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor PU.1
Alternative name(s):
31 kDa-transforming protein
Gene namesi
Name:SPI1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000066336.11
HGNCiHGNC:11241 SPI1
MIMi165170 gene
neXtProtiNX_P17947

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

Organism-specific databases

DisGeNETi6688
OpenTargetsiENSG00000066336
PharmGKBiPA36071

Polymorphism and mutation databases

BioMutaiSPI1
DMDMi60415923

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002041321 – 270Transcription factor PU.1Add BLAST270

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei140PhosphoserineCombined sources1
Modified residuei146PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP17947
PeptideAtlasiP17947
PRIDEiP17947

PTM databases

iPTMnetiP17947
PhosphoSitePlusiP17947

Expressioni

Inductioni

Highly expressed in both FV-P and FV-A-induced erythro-leukemia cell lines that have undergone rearrangements of the SPI1 gene due to the insertion of SFFV. Negatively regulated by microRNA-155 (miR-155).1 Publication

Gene expression databases

BgeeiENSG00000066336
CleanExiHS_SPI1
ExpressionAtlasiP17947 baseline and differential
GenevisibleiP17947 HS

Organism-specific databases

HPAiCAB004079
HPA044653

Interactioni

Subunit structurei

Binds DNA as a monomer. Interacts with CEBPD and NONO (By similarity). Interacts with RUNX1 and SPIB. Interacts with GFI1; the interaction represses SPI1 transcriptional activity. Interacts with CEBPE (PubMed:26019275).By similarity4 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi112566, 41 interactors
CORUMiP17947
DIPiDIP-953N
IntActiP17947, 11 interactors
MINTiP17947
STRINGi9606.ENSP00000227163

Structurei

3D structure databases

ProteinModelPortaliP17947
SMRiP17947
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ETS family.Curated

Phylogenomic databases

eggNOGiKOG3805 Eukaryota
ENOG410XSXU LUCA
GeneTreeiENSGT00390000015212
HOGENOMiHOG000095520
HOVERGENiHBG002474
InParanoidiP17947
KOiK09438
OMAiPRMCLPY
OrthoDBiEOG091G0DTY
PhylomeDBiP17947
TreeFamiTF352494

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR000418 Ets_dom
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00178 Ets, 1 hit
PRINTSiPR00454 ETSDOMAIN
SMARTiView protein in SMART
SM00413 ETS, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS00345 ETS_DOMAIN_1, 1 hit
PS00346 ETS_DOMAIN_2, 1 hit
PS50061 ETS_DOMAIN_3, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P17947-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLQACKMEGF PLVPPPSEDL VPYDTDLYQR QTHEYYPYLS SDGESHSDHY
60 70 80 90 100
WDFHPHHVHS EFESFAENNF TELQSVQPPQ LQQLYRHMEL EQMHVLDTPM
110 120 130 140 150
VPPHPSLGHQ VSYLPRMCLQ YPSLSPAQPS SDEEEGERQS PPLEVSDGEA
160 170 180 190 200
DGLEPGPGLL PGETGSKKKI RLYQFLLDLL RSGDMKDSIW WVDKDKGTFQ
210 220 230 240 250
FSSKHKEALA HRWGIQKGNR KKMTYQKMAR ALRNYGKTGE VKKVKKKLTY
260 270
QFSGEVLGRG GLAERRHPPH
Length:270
Mass (Da):31,083
Last modified:February 1, 2005 - v2
Checksum:i9592DAA1D85053ED
GO
Isoform 2 (identifier: P17947-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     15-15: P → PQ

Show »
Length:271
Mass (Da):31,211
Checksum:iF69ED4D4C3F77318
GO

Sequence cautioni

The sequence CAA36281 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03869015P → PQ in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52056 mRNA Translation: CAA36281.1 Different initiation.
AL532058 mRNA No translation available.
AC090559 Genomic DNA No translation available.
AC090582 Genomic DNA No translation available.
CCDSiCCDS44591.1 [P17947-2]
CCDS7933.2 [P17947-1]
PIRiS60367
RefSeqiNP_001074016.1, NM_001080547.1 [P17947-2]
NP_003111.2, NM_003120.2 [P17947-1]
UniGeneiHs.502511

Genome annotation databases

EnsembliENST00000227163; ENSP00000227163; ENSG00000066336 [P17947-2]
ENST00000378538; ENSP00000367799; ENSG00000066336 [P17947-1]
GeneIDi6688
KEGGihsa:6688
UCSCiuc001nfb.2 human [P17947-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSPI1_HUMAN
AccessioniPrimary (citable) accession number: P17947
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: February 1, 2005
Last modified: May 23, 2018
This is version 180 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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