Reviewed, UniProtKB/Swiss-Prot P17947 (SPI1_HUMAN)

Names and origin

Protein names

Recommended name:
    Transcription factor PU.1
Alternative name(s):
    31 kDa-transforming protein

Gene names

Name:

SPI1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	270 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing By similarity.
Subunit structure	Binds DNA as a monomer. Interacts with RUNX1 and SPIB. Interacts with CEBPD and NONO By similarity.
Subcellular location	Nucleus.
Induction	Highly expressed in both FV-P and FV-A-induced erythro-leukemia cell lines that have undergone rearrangements of the Spi-1 gene due to the insertion of SFFV.
Sequence similarities	Belongs to the ETS family. Contains 1 ETS DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Disease	Proto-oncogene
Ligand	DNA-binding RNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	negative regulation of transcription from RNA polymerase II promoter Traceable author statement. Source: ProtInc positive regulation of gene-specific transcription Inferred from direct assay. Source: UniProtKB regulation of erythrocyte differentiation Inferred from mutant phenotype. Source: UniProtKB transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Ref.4 Inferred from physical interaction. Source: UniProtKB sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro transcription factor activity Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 270	270	Transcription factor PU.1		PRO_0000204132
Regions
	DNA binding	170 – 253	84	ETS
Amino acid modifications
	Modified residue	146	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P17947-1 [UniParc]. Last modified February 1, 2005. Version 2. Checksum: 9592DAA1D85053ED	FASTA	270	31,083
	10 20 30 40 50 60 MLQACKMEGF PLVPPPSEDL VPYDTDLYQR QTHEYYPYLS SDGESHSDHY WDFHPHHVHS 70 80 90 100 110 120 EFESFAENNF TELQSVQPPQ LQQLYRHMEL EQMHVLDTPM VPPHPSLGHQ VSYLPRMCLQ 130 140 150 160 170 180 YPSLSPAQPS SDEEEGERQS PPLEVSDGEA DGLEPGPGLL PGETGSKKKI RLYQFLLDLL 190 200 210 220 230 240 RSGDMKDSIW WVDKDKGTFQ FSSKHKEALA HRWGIQKGNR KKMTYQKMAR ALRNYGKTGE 250 260 270 VKKVKKKLTY QFSGEVLGRG GLAERRHPPH

References

[1]	"The human homologue of the putative proto-oncogene Spi-1: characterization and expression in tumors." Ray D., Culine S., Tavitian A., Moreau-Gachelin F. Oncogene 5:663-668(1990) [PubMed: 1693183] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Erratum Ray D., Culine S., Tavitian A., Moreau-Gachelin F. Oncogene 5:1611-1612(1990) Cited for: SEQUENCE REVISION.
[3]	"SPI-B activates transcription via a unique proline, serine, and threonine domain and exhibits DNA binding affinity differences from PU.1." Rao S., Matsumura A., Yoon J., Simon M.C. J. Biol. Chem. 274:11115-11124(1999) [PubMed: 10196196] [Abstract] Cited for: INTERACTION WITH SPIB.
[4]	"Functional and physical interactions between AML1 proteins and an ETS protein, MEF: implications for the pathogenesis of t(8;21)-positive leukemias." Mao S., Frank R.C., Zhang J., Miyazaki Y., Nimer S.D. Mol. Cell. Biol. 19:3635-3644(1999) [PubMed: 10207087] [Abstract] Cited for: INTERACTION WITH RUNX1.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases
EMBLGenBankDDBJ	X52056 mRNA. Translation: CAA36281.1. Different initiation.
IPI	IPI00018333.
PIR	S60367.
RefSeq	NP_003111.2.
UniGene	Hs.502511 Hs.524906
3D structure databases
HSSP	HSSP built from PDB template 1PUE based on UniProtKB P17433.
SMR	P17947. Positions 169-257.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:953N.
PTM databases
PhosphoSite	P17947.
Genome annotation databases
Ensembl	ENSG00000066336. Homo sapiens. [Contig view]
GeneID	6688.
Organism-specific databases
GeneCards	GC11M047332.
H-InvDB	HIX0035922.
HGNC	HGNC:11241. SPI1.
HPA	CAB004079.
MIM	165170. gene.
PharmGKB	PA36071.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	P17947.
HOVERGEN	P17947.
OMA	P17947. PRMCLPY.
Enzyme and pathway databases
Pathway_Interaction_DB	il4_2pathway. IL4-mediated signaling events.
Gene expression databases
ArrayExpress	P17947.
Bgee	P17947.
CleanEx	HS_SPI1.
GermOnline	ENSG00000066336. Homo sapiens.
Family and domain databases
InterPro	IPR000418. Ets. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF00178. Ets. 1 hit. [Graphical view]
PRINTS	PR00454. ETSDOMAIN.
SMART	SM00413. ETS. 1 hit. [Graphical view]
PROSITE	PS00345. ETS_DOMAIN_1. 1 hit. PS00346. ETS_DOMAIN_2. 1 hit. PS50061. ETS_DOMAIN_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	26063.
SOURCE	Search...

Entry information

Entry name

SPI1_HUMAN

Accession

Primary (citable) accession number: P17947

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	February 1, 2005
Last modified:	June 16, 2009
This is version 96 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)