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P17947

- SPI1_HUMAN

UniProt

P17947 - SPI1_HUMAN

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Protein

Transcription factor PU.1

Gene

SPI1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi170 – 25384ETSPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. core promoter binding Source: UniProtKB
  2. NFAT protein binding Source: BHF-UCL
  3. RNA binding Source: UniProtKB-KW
  4. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: Ensembl
  5. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: Ensembl
  6. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: Ensembl
  7. RNA polymerase II core promoter sequence-specific DNA binding Source: Ensembl
  8. RNA polymerase II distal enhancer sequence-specific DNA binding Source: BHF-UCL
  9. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Source: BHF-UCL
  10. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: Ensembl
  11. RNA polymerase II transcription factor binding Source: BHF-UCL
  12. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: RefGenome
  13. sequence-specific DNA binding transcription factor activity Source: BHF-UCL

GO - Biological processi

  1. anatomical structure regression Source: Ensembl
  2. apoptotic process involved in patterning of blood vessels Source: Ensembl
  3. erythrocyte differentiation Source: Ensembl
  4. granulocyte differentiation Source: Ensembl
  5. histone H3 acetylation Source: UniProtKB
  6. hypermethylation of CpG island Source: UniProtKB
  7. lymphocyte differentiation Source: Ensembl
  8. lymphoid progenitor cell differentiation Source: Ensembl
  9. macrophage differentiation Source: Ensembl
  10. myeloid dendritic cell differentiation Source: Ensembl
  11. negative regulation of gene expression, epigenetic Source: UniProtKB
  12. negative regulation of histone H4 acetylation Source: UniProtKB
  13. negative regulation of MHC class II biosynthetic process Source: Ensembl
  14. negative regulation of transcription, DNA-templated Source: UniProtKB
  15. negative regulation of transcription from RNA polymerase II promoter Source: ProtInc
  16. positive regulation of transcription, DNA-templated Source: BHF-UCL
  17. positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  18. regulation of erythrocyte differentiation Source: BHF-UCL
  19. regulation of transcription from RNA polymerase II promoter Source: RefGenome
  20. somatic stem cell maintenance Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, RNA-binding

Enzyme and pathway databases

SignaLinkiP17947.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor PU.1
Alternative name(s):
31 kDa-transforming protein
Gene namesi
Name:SPI1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 11

Organism-specific databases

HGNCiHGNC:11241. SPI1.

Subcellular locationi

Nucleus 1 PublicationPROSITE-ProRule annotation

GO - Cellular componenti

  1. nuclear chromatin Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

Organism-specific databases

PharmGKBiPA36071.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 270270Transcription factor PU.1PRO_0000204132Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei146 – 1461PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP17947.
PRIDEiP17947.

PTM databases

PhosphoSiteiP17947.

Expressioni

Inductioni

Highly expressed in both FV-P and FV-A-induced erythro-leukemia cell lines that have undergone rearrangements of the SPI1 gene due to the insertion of SFFV. Negatively regulated by microRNA-155 (miR-155).1 Publication

Gene expression databases

BgeeiP17947.
CleanExiHS_SPI1.
ExpressionAtlasiP17947. baseline and differential.
GenevestigatoriP17947.

Organism-specific databases

HPAiCAB004079.
HPA044653.

Interactioni

Subunit structurei

Binds DNA as a monomer. Interacts with CEBPD and NONO (By similarity). Interacts with RUNX1 and SPIB. Interacts with GFI1; the interaction represses SPI1 transcriptional activity.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HOXA10P312602EBI-2293548,EBI-2293516
PML-RARQ1515620EBI-2293548,EBI-867256
PRKDCP785272EBI-2293548,EBI-352053

Protein-protein interaction databases

BioGridi112566. 39 interactions.
DIPiDIP-953N.
IntActiP17947. 10 interactions.
STRINGi9606.ENSP00000227163.

Structurei

3D structure databases

ProteinModelPortaliP17947.
SMRiP17947. Positions 169-257.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ETS family.Curated
Contains 1 ETS DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG237824.
GeneTreeiENSGT00390000015212.
HOGENOMiHOG000095520.
HOVERGENiHBG002474.
InParanoidiP17947.
KOiK09438.
OMAiPRMCLPY.
OrthoDBiEOG78M02J.
PhylomeDBiP17947.
TreeFamiTF352494.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000418. Ets_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00178. Ets. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
[Graphical view]
PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P17947-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLQACKMEGF PLVPPPSEDL VPYDTDLYQR QTHEYYPYLS SDGESHSDHY
60 70 80 90 100
WDFHPHHVHS EFESFAENNF TELQSVQPPQ LQQLYRHMEL EQMHVLDTPM
110 120 130 140 150
VPPHPSLGHQ VSYLPRMCLQ YPSLSPAQPS SDEEEGERQS PPLEVSDGEA
160 170 180 190 200
DGLEPGPGLL PGETGSKKKI RLYQFLLDLL RSGDMKDSIW WVDKDKGTFQ
210 220 230 240 250
FSSKHKEALA HRWGIQKGNR KKMTYQKMAR ALRNYGKTGE VKKVKKKLTY
260 270
QFSGEVLGRG GLAERRHPPH
Length:270
Mass (Da):31,083
Last modified:February 1, 2005 - v2
Checksum:i9592DAA1D85053ED
GO
Isoform 2 (identifier: P17947-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     15-15: P → PQ

Show »
Length:271
Mass (Da):31,211
Checksum:iF69ED4D4C3F77318
GO

Sequence cautioni

The sequence CAA36281.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei15 – 151P → PQ in isoform 2. 1 PublicationVSP_038690

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52056 mRNA. Translation: CAA36281.1. Different initiation.
AL532058 mRNA. No translation available.
AC090559 Genomic DNA. No translation available.
AC090582 Genomic DNA. No translation available.
CCDSiCCDS44591.1. [P17947-2]
CCDS7933.2. [P17947-1]
PIRiS60367.
RefSeqiNP_001074016.1. NM_001080547.1. [P17947-2]
NP_003111.2. NM_003120.2. [P17947-1]
UniGeneiHs.502511.

Genome annotation databases

EnsembliENST00000227163; ENSP00000227163; ENSG00000066336. [P17947-2]
ENST00000378538; ENSP00000367799; ENSG00000066336. [P17947-1]
GeneIDi6688.
KEGGihsa:6688.
UCSCiuc001nfb.1. human. [P17947-2]
uc001nfc.1. human. [P17947-1]

Polymorphism databases

DMDMi60415923.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52056 mRNA. Translation: CAA36281.1 . Different initiation.
AL532058 mRNA. No translation available.
AC090559 Genomic DNA. No translation available.
AC090582 Genomic DNA. No translation available.
CCDSi CCDS44591.1. [P17947-2 ]
CCDS7933.2. [P17947-1 ]
PIRi S60367.
RefSeqi NP_001074016.1. NM_001080547.1. [P17947-2 ]
NP_003111.2. NM_003120.2. [P17947-1 ]
UniGenei Hs.502511.

3D structure databases

ProteinModelPortali P17947.
SMRi P17947. Positions 169-257.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 112566. 39 interactions.
DIPi DIP-953N.
IntActi P17947. 10 interactions.
STRINGi 9606.ENSP00000227163.

PTM databases

PhosphoSitei P17947.

Polymorphism databases

DMDMi 60415923.

Proteomic databases

PaxDbi P17947.
PRIDEi P17947.

Protocols and materials databases

DNASUi 6688.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000227163 ; ENSP00000227163 ; ENSG00000066336 . [P17947-2 ]
ENST00000378538 ; ENSP00000367799 ; ENSG00000066336 . [P17947-1 ]
GeneIDi 6688.
KEGGi hsa:6688.
UCSCi uc001nfb.1. human. [P17947-2 ]
uc001nfc.1. human. [P17947-1 ]

Organism-specific databases

CTDi 6688.
GeneCardsi GC11M049902.
HGNCi HGNC:11241. SPI1.
HPAi CAB004079.
HPA044653.
MIMi 165170. gene.
neXtProti NX_P17947.
PharmGKBi PA36071.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG237824.
GeneTreei ENSGT00390000015212.
HOGENOMi HOG000095520.
HOVERGENi HBG002474.
InParanoidi P17947.
KOi K09438.
OMAi PRMCLPY.
OrthoDBi EOG78M02J.
PhylomeDBi P17947.
TreeFami TF352494.

Enzyme and pathway databases

SignaLinki P17947.

Miscellaneous databases

ChiTaRSi SPI1. human.
GeneWikii SPI1.
GenomeRNAii 6688.
NextBioi 26063.
PROi P17947.
SOURCEi Search...

Gene expression databases

Bgeei P17947.
CleanExi HS_SPI1.
ExpressionAtlasi P17947. baseline and differential.
Genevestigatori P17947.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
InterProi IPR000418. Ets_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF00178. Ets. 1 hit.
[Graphical view ]
PRINTSi PR00454. ETSDOMAIN.
SMARTi SM00413. ETS. 1 hit.
[Graphical view ]
PROSITEi PS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The human homologue of the putative proto-oncogene Spi-1: characterization and expression in tumors."
    Ray D., Culine S., Tavitian A., Moreau-Gachelin F.
    Oncogene 5:663-668(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. Erratum
    Ray D., Culine S., Tavitian A., Moreau-Gachelin F.
    Oncogene 5:1611-1612(1990)
    Cited for: SEQUENCE REVISION.
  3. "Full-length cDNA libraries and normalization."
    Li W.B., Gruber C., Jessee J., Polayes D.
    Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Fetal liver.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "SPI-B activates transcription via a unique proline, serine, and threonine domain and exhibits DNA binding affinity differences from PU.1."
    Rao S., Matsumura A., Yoon J., Simon M.C.
    J. Biol. Chem. 274:11115-11124(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SPIB.
  6. "Functional and physical interactions between AML1 proteins and an ETS protein, MEF: implications for the pathogenesis of t(8;21)-positive leukemias."
    Mao S., Frank R.C., Zhang J., Miyazaki Y., Nimer S.D.
    Mol. Cell. Biol. 19:3635-3644(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RUNX1.
  7. "The transcriptional repressor GFI-1 antagonizes PU.1 activity through protein-protein interaction."
    Dahl R., Iyer S.R., Owens K.S., Cuylear D.D., Simon M.C.
    J. Biol. Chem. 282:6473-6483(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GFI1.
  8. "BCL6 positively regulates AID and germinal center gene expression via repression of miR-155."
    Basso K., Schneider C., Shen Q., Holmes A.B., Setty M., Leslie C., Dalla-Favera R.
    J. Exp. Med. 209:2455-2465(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiSPI1_HUMAN
AccessioniPrimary (citable) accession number: P17947
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: February 1, 2005
Last modified: November 26, 2014
This is version 154 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3