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P17947

- SPI1_HUMAN

UniProt

P17947 - SPI1_HUMAN

Protein

Transcription factor PU.1

Gene

SPI1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 152 (01 Oct 2014)
      Sequence version 2 (01 Feb 2005)
      Previous versions | rss
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    Functioni

    Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi170 – 25384ETSPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. core promoter binding Source: UniProtKB
    2. NFAT protein binding Source: BHF-UCL
    3. protein binding Source: UniProtKB
    4. RNA binding Source: UniProtKB-KW
    5. RNA polymerase II distal enhancer sequence-specific DNA binding Source: BHF-UCL
    6. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Source: BHF-UCL
    7. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: Ensembl
    8. RNA polymerase II transcription factor binding Source: BHF-UCL
    9. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: RefGenome
    10. sequence-specific DNA binding transcription factor activity Source: BHF-UCL

    GO - Biological processi

    1. anatomical structure regression Source: Ensembl
    2. apoptotic process involved in patterning of blood vessels Source: Ensembl
    3. erythrocyte differentiation Source: Ensembl
    4. granulocyte differentiation Source: Ensembl
    5. histone H3 acetylation Source: UniProtKB
    6. hypermethylation of CpG island Source: UniProtKB
    7. lymphocyte differentiation Source: Ensembl
    8. lymphoid progenitor cell differentiation Source: Ensembl
    9. macrophage differentiation Source: Ensembl
    10. myeloid dendritic cell differentiation Source: Ensembl
    11. negative regulation of gene expression, epigenetic Source: UniProtKB
    12. negative regulation of histone H4 acetylation Source: UniProtKB
    13. negative regulation of MHC class II biosynthetic process Source: Ensembl
    14. negative regulation of transcription, DNA-templated Source: UniProtKB
    15. negative regulation of transcription from RNA polymerase II promoter Source: ProtInc
    16. positive regulation of transcription, DNA-templated Source: BHF-UCL
    17. positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
    18. regulation of erythrocyte differentiation Source: BHF-UCL
    19. regulation of transcription from RNA polymerase II promoter Source: RefGenome
    20. somatic stem cell maintenance Source: Ensembl
    21. transcription from RNA polymerase II promoter Source: GOC

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, RNA-binding

    Enzyme and pathway databases

    SignaLinkiP17947.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription factor PU.1
    Alternative name(s):
    31 kDa-transforming protein
    Gene namesi
    Name:SPI1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:11241. SPI1.

    Subcellular locationi

    Nucleus 1 PublicationPROSITE-ProRule annotation

    GO - Cellular componenti

    1. nuclear chromatin Source: BHF-UCL

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Keywords - Diseasei

    Proto-oncogene

    Organism-specific databases

    PharmGKBiPA36071.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 270270Transcription factor PU.1PRO_0000204132Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei146 – 1461PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP17947.
    PRIDEiP17947.

    PTM databases

    PhosphoSiteiP17947.

    Expressioni

    Inductioni

    Highly expressed in both FV-P and FV-A-induced erythro-leukemia cell lines that have undergone rearrangements of the SPI1 gene due to the insertion of SFFV. Negatively regulated by microRNA-155 (miR-155).1 Publication

    Gene expression databases

    ArrayExpressiP17947.
    BgeeiP17947.
    CleanExiHS_SPI1.
    GenevestigatoriP17947.

    Organism-specific databases

    HPAiCAB004079.
    HPA044653.

    Interactioni

    Subunit structurei

    Binds DNA as a monomer. Interacts with CEBPD and NONO By similarity. Interacts with RUNX1 and SPIB. Interacts with GFI1; the interaction represses SPI1 transcriptional activity.By similarity3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    HOXA10P312602EBI-2293548,EBI-2293516
    PML-RARQ1515620EBI-2293548,EBI-867256
    PRKDCP785272EBI-2293548,EBI-352053

    Protein-protein interaction databases

    BioGridi112566. 39 interactions.
    DIPiDIP-953N.
    IntActiP17947. 10 interactions.
    STRINGi9606.ENSP00000227163.

    Structurei

    3D structure databases

    ProteinModelPortaliP17947.
    SMRiP17947. Positions 169-257.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ETS family.Curated
    Contains 1 ETS DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG237824.
    HOGENOMiHOG000095520.
    HOVERGENiHBG002474.
    InParanoidiP17947.
    KOiK09438.
    OMAiPRMCLPY.
    OrthoDBiEOG78M02J.
    PhylomeDBiP17947.
    TreeFamiTF352494.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    InterProiIPR000418. Ets_dom.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00178. Ets. 1 hit.
    [Graphical view]
    PRINTSiPR00454. ETSDOMAIN.
    SMARTiSM00413. ETS. 1 hit.
    [Graphical view]
    PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
    PS00346. ETS_DOMAIN_2. 1 hit.
    PS50061. ETS_DOMAIN_3. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P17947-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MLQACKMEGF PLVPPPSEDL VPYDTDLYQR QTHEYYPYLS SDGESHSDHY    50
    WDFHPHHVHS EFESFAENNF TELQSVQPPQ LQQLYRHMEL EQMHVLDTPM 100
    VPPHPSLGHQ VSYLPRMCLQ YPSLSPAQPS SDEEEGERQS PPLEVSDGEA 150
    DGLEPGPGLL PGETGSKKKI RLYQFLLDLL RSGDMKDSIW WVDKDKGTFQ 200
    FSSKHKEALA HRWGIQKGNR KKMTYQKMAR ALRNYGKTGE VKKVKKKLTY 250
    QFSGEVLGRG GLAERRHPPH 270
    Length:270
    Mass (Da):31,083
    Last modified:February 1, 2005 - v2
    Checksum:i9592DAA1D85053ED
    GO
    Isoform 2 (identifier: P17947-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         15-15: P → PQ

    Show »
    Length:271
    Mass (Da):31,211
    Checksum:iF69ED4D4C3F77318
    GO

    Sequence cautioni

    The sequence CAA36281.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei15 – 151P → PQ in isoform 2. 1 PublicationVSP_038690

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X52056 mRNA. Translation: CAA36281.1. Different initiation.
    AL532058 mRNA. No translation available.
    AC090559 Genomic DNA. No translation available.
    AC090582 Genomic DNA. No translation available.
    CCDSiCCDS44591.1. [P17947-2]
    CCDS7933.2. [P17947-1]
    PIRiS60367.
    RefSeqiNP_001074016.1. NM_001080547.1. [P17947-2]
    NP_003111.2. NM_003120.2. [P17947-1]
    UniGeneiHs.502511.

    Genome annotation databases

    EnsembliENST00000227163; ENSP00000227163; ENSG00000066336. [P17947-2]
    ENST00000378538; ENSP00000367799; ENSG00000066336. [P17947-1]
    GeneIDi6688.
    KEGGihsa:6688.
    UCSCiuc001nfb.1. human. [P17947-2]
    uc001nfc.1. human. [P17947-1]

    Polymorphism databases

    DMDMi60415923.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Web resourcesi

    Atlas of Genetics and Cytogenetics in Oncology and Haematology

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X52056 mRNA. Translation: CAA36281.1 . Different initiation.
    AL532058 mRNA. No translation available.
    AC090559 Genomic DNA. No translation available.
    AC090582 Genomic DNA. No translation available.
    CCDSi CCDS44591.1. [P17947-2 ]
    CCDS7933.2. [P17947-1 ]
    PIRi S60367.
    RefSeqi NP_001074016.1. NM_001080547.1. [P17947-2 ]
    NP_003111.2. NM_003120.2. [P17947-1 ]
    UniGenei Hs.502511.

    3D structure databases

    ProteinModelPortali P17947.
    SMRi P17947. Positions 169-257.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 112566. 39 interactions.
    DIPi DIP-953N.
    IntActi P17947. 10 interactions.
    STRINGi 9606.ENSP00000227163.

    PTM databases

    PhosphoSitei P17947.

    Polymorphism databases

    DMDMi 60415923.

    Proteomic databases

    PaxDbi P17947.
    PRIDEi P17947.

    Protocols and materials databases

    DNASUi 6688.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000227163 ; ENSP00000227163 ; ENSG00000066336 . [P17947-2 ]
    ENST00000378538 ; ENSP00000367799 ; ENSG00000066336 . [P17947-1 ]
    GeneIDi 6688.
    KEGGi hsa:6688.
    UCSCi uc001nfb.1. human. [P17947-2 ]
    uc001nfc.1. human. [P17947-1 ]

    Organism-specific databases

    CTDi 6688.
    GeneCardsi GC11M048800.
    HGNCi HGNC:11241. SPI1.
    HPAi CAB004079.
    HPA044653.
    MIMi 165170. gene.
    neXtProti NX_P17947.
    PharmGKBi PA36071.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG237824.
    HOGENOMi HOG000095520.
    HOVERGENi HBG002474.
    InParanoidi P17947.
    KOi K09438.
    OMAi PRMCLPY.
    OrthoDBi EOG78M02J.
    PhylomeDBi P17947.
    TreeFami TF352494.

    Enzyme and pathway databases

    SignaLinki P17947.

    Miscellaneous databases

    GeneWikii SPI1.
    GenomeRNAii 6688.
    NextBioi 26063.
    PROi P17947.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P17947.
    Bgeei P17947.
    CleanExi HS_SPI1.
    Genevestigatori P17947.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    InterProi IPR000418. Ets_dom.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00178. Ets. 1 hit.
    [Graphical view ]
    PRINTSi PR00454. ETSDOMAIN.
    SMARTi SM00413. ETS. 1 hit.
    [Graphical view ]
    PROSITEi PS00345. ETS_DOMAIN_1. 1 hit.
    PS00346. ETS_DOMAIN_2. 1 hit.
    PS50061. ETS_DOMAIN_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The human homologue of the putative proto-oncogene Spi-1: characterization and expression in tumors."
      Ray D., Culine S., Tavitian A., Moreau-Gachelin F.
      Oncogene 5:663-668(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. Erratum
      Ray D., Culine S., Tavitian A., Moreau-Gachelin F.
      Oncogene 5:1611-1612(1990)
      Cited for: SEQUENCE REVISION.
    3. "Full-length cDNA libraries and normalization."
      Li W.B., Gruber C., Jessee J., Polayes D.
      Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Fetal liver.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "SPI-B activates transcription via a unique proline, serine, and threonine domain and exhibits DNA binding affinity differences from PU.1."
      Rao S., Matsumura A., Yoon J., Simon M.C.
      J. Biol. Chem. 274:11115-11124(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SPIB.
    6. "Functional and physical interactions between AML1 proteins and an ETS protein, MEF: implications for the pathogenesis of t(8;21)-positive leukemias."
      Mao S., Frank R.C., Zhang J., Miyazaki Y., Nimer S.D.
      Mol. Cell. Biol. 19:3635-3644(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RUNX1.
    7. "The transcriptional repressor GFI-1 antagonizes PU.1 activity through protein-protein interaction."
      Dahl R., Iyer S.R., Owens K.S., Cuylear D.D., Simon M.C.
      J. Biol. Chem. 282:6473-6483(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH GFI1.
    8. "BCL6 positively regulates AID and germinal center gene expression via repression of miR-155."
      Basso K., Schneider C., Shen Q., Holmes A.B., Setty M., Leslie C., Dalla-Favera R.
      J. Exp. Med. 209:2455-2465(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiSPI1_HUMAN
    AccessioniPrimary (citable) accession number: P17947
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: February 1, 2005
    Last modified: October 1, 2014
    This is version 152 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3