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Protein

Galectin-3

Gene

LGALS3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.By similarity3 Publications

GO - Molecular functioni

  • carbohydrate binding Source: ProtInc
  • chemoattractant activity Source: BHF-UCL
  • IgE binding Source: BHF-UCL
  • laminin binding Source: BHF-UCL
  • oligosaccharide binding Source: UniProtKB
  • RNA binding Source: UniProtKB

GO - Biological processi

  • antimicrobial humoral immune response mediated by antimicrobial peptide Source: UniProtKB
  • eosinophil chemotaxis Source: BHF-UCL
  • epithelial cell differentiation Source: UniProtKB
  • innate immune response Source: UniProtKB-KW
  • killing of cells of other organism Source: UniProtKB
  • macrophage chemotaxis Source: BHF-UCL
  • monocyte chemotaxis Source: BHF-UCL
  • mononuclear cell migration Source: BHF-UCL
  • mRNA processing Source: UniProtKB-KW
  • negative regulation of endocytosis Source: BHF-UCL
  • negative regulation of extrinsic apoptotic signaling pathway Source: BHF-UCL
  • negative regulation of immunological synapse formation Source: BHF-UCL
  • negative regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell Source: BHF-UCL
  • negative regulation of T cell receptor signaling pathway Source: BHF-UCL
  • neutrophil chemotaxis Source: BHF-UCL
  • neutrophil degranulation Source: Reactome
  • positive chemotaxis Source: BHF-UCL
  • positive regulation of calcium ion import Source: BHF-UCL
  • positive regulation of mononuclear cell migration Source: BHF-UCL
  • regulation of extrinsic apoptotic signaling pathway via death domain receptors Source: BHF-UCL
  • regulation of myeloid cell differentiation Source: Reactome
  • regulation of T cell apoptotic process Source: BHF-UCL
  • regulation of T cell proliferation Source: BHF-UCL
  • RNA splicing Source: UniProtKB-KW

Keywordsi

Molecular functionIgE-binding protein
Biological processDifferentiation, Immunity, Innate immunity, mRNA processing, mRNA splicing
LigandLectin

Enzyme and pathway databases

ReactomeiR-HSA-6798695 Neutrophil degranulation
R-HSA-879415 Advanced glycosylation endproduct receptor signaling
R-HSA-8939246 RUNX1 regulates transcription of genes involved in differentiation of myeloid cells
R-HSA-8941333 RUNX2 regulates genes involved in differentiation of myeloid cells
SIGNORiP17931

Names & Taxonomyi

Protein namesi
Recommended name:
Galectin-3
Short name:
Gal-3
Alternative name(s):
35 kDa lectin
Carbohydrate-binding protein 35
Short name:
CBP 35
Galactose-specific lectin 3
Galactoside-binding protein
Short name:
GALBP
IgE-binding protein
L-31
Laminin-binding protein
Lectin L-29
Mac-2 antigen
Gene namesi
Name:LGALS3
Synonyms:MAC2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000131981.15
HGNCiHGNC:6563 LGALS3
MIMi153619 gene
neXtProtiNX_P17931

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Secreted, Spliceosome

Pathology & Biotechi

Organism-specific databases

DisGeNETi3958
OpenTargetsiENSG00000131981
PharmGKBiPA30340

Chemistry databases

ChEMBLiCHEMBL4531

Polymorphism and mutation databases

BioMutaiLGALS3
DMDMi215274262

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000769302 – 250Galectin-3Add BLAST249

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei6Phosphoserine1 Publication1
Modified residuei12Phosphoserine1 Publication1
Disulfide bondi173InterchainBy similarity
Modified residuei188PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Disulfide bond, Phosphoprotein

Proteomic databases

EPDiP17931
MaxQBiP17931
PaxDbiP17931
PeptideAtlasiP17931
PRIDEiP17931

2D gel databases

DOSAC-COBS-2DPAGEiP17931
REPRODUCTION-2DPAGEiIPI00465431
UCD-2DPAGEiP17931

PTM databases

iPTMnetiP17931
PhosphoSitePlusiP17931

Miscellaneous databases

PMAP-CutDBiQ6IBA7

Expressioni

Tissue specificityi

A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Expressed in fetal membranes.1 Publication

Gene expression databases

BgeeiENSG00000131981
CleanExiHS_LGALS3
ExpressionAtlasiP17931 baseline and differential
GenevisibleiP17931 HS

Organism-specific databases

HPAiCAB005191
HPA003162

Interactioni

Subunit structurei

Probably forms homo- or heterodimers. Interacts with DMBT1 (By similarity). Interacts with CD6 and ALCAM (PubMed:24945728). Forms a complex with the ITGA3, ITGB1 and CSPG4. Interacts with LGALS3BP, LYPD3, CYHR1 and UACA.By similarity3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • chemoattractant activity Source: BHF-UCL
  • laminin binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi110149, 210 interactors
CORUMiP17931
DIPiDIP-45623N
IntActiP17931, 61 interactors
MINTiP17931
STRINGi9606.ENSP00000254301

Chemistry databases

BindingDBiP17931

Structurei

Secondary structure

1250
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi116 – 121Combined sources6
Beta strandi130 – 138Combined sources9
Beta strandi144 – 151Combined sources8
Beta strandi154 – 165Combined sources12
Beta strandi168 – 177Combined sources10
Beta strandi185 – 187Combined sources3
Beta strandi197 – 204Combined sources8
Beta strandi206 – 213Combined sources8
Beta strandi216 – 222Combined sources7
Helixi228 – 230Combined sources3
Beta strandi233 – 249Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A3KX-ray2.10A114-250[»]
1KJLX-ray1.40A105-250[»]
1KJRX-ray1.55A105-250[»]
2NMNX-ray2.45A113-250[»]
2NMOX-ray1.35A113-250[»]
2NN8X-ray1.35A113-250[»]
2XG3X-ray1.20A114-250[»]
3AYAX-ray2.00A/B117-250[»]
3AYCX-ray1.80A/B117-250[»]
3AYDX-ray1.90A117-250[»]
3AYEX-ray2.00A/B117-250[»]
3T1LX-ray1.60A108-250[»]
3T1MX-ray1.55A108-250[»]
3ZSJX-ray0.86A113-250[»]
3ZSKX-ray0.90A114-250[»]
3ZSLX-ray1.08A114-250[»]
3ZSMX-ray1.25A114-250[»]
4BLIX-ray1.08A114-250[»]
4BLJX-ray1.20A114-250[»]
4BM8X-ray0.96A114-250[»]
4JC1X-ray1.50A108-250[»]
4JCKX-ray1.15A108-250[»]
4LBJX-ray1.80A114-250[»]
4LBKX-ray1.60A114-250[»]
4LBLX-ray1.58A114-250[»]
4LBMX-ray1.55A112-250[»]
4LBNX-ray1.70A112-250[»]
4LBOX-ray1.65A113-250[»]
4R9AX-ray1.20A111-250[»]
4R9BX-ray1.20A111-250[»]
4R9CX-ray1.19A111-250[»]
4R9DX-ray1.24A111-250[»]
4RL7X-ray2.00A111-250[»]
4XBNX-ray2.21A113-250[»]
5E88X-ray1.60A114-250[»]
5E89X-ray1.50A114-250[»]
5E8AX-ray1.50A114-250[»]
5EXOX-ray1.50A112-250[»]
5H9PX-ray2.04A113-250[»]
5H9RX-ray1.58A113-250[»]
5IUQX-ray1.12A113-250[»]
5NF7X-ray1.59A106-250[»]
5NF9X-ray1.87A106-250[»]
5NFAX-ray1.59A106-250[»]
5NFBX-ray1.59A106-250[»]
5NFCX-ray1.59A106-250[»]
ProteinModelPortaliP17931
SMRiP17931
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP17931

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati36 – 4419
Repeati45 – 5329
Repeati54 – 6239
Repeati63 – 694; approximate7
Repeati70 – 7859
Repeati79 – 886; approximate10
Repeati89 – 1007; approximateAdd BLAST12
Repeati101 – 1098; approximate9
Domaini118 – 248GalectinPROSITE-ProRule annotationAdd BLAST131

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni36 – 1098 X 9 AA tandem repeats of Y-P-G-X(3)-P-G-AAdd BLAST74
Regioni181 – 187Beta-galactoside bindingBy similarity7

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi226 – 241Nuclear export signalBy similarityAdd BLAST16

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3587 Eukaryota
ENOG4111EA0 LUCA
GeneTreeiENSGT00760000119105
HOVERGENiHBG006255
InParanoidiP17931
KOiK06831
OMAiSASHAMI
OrthoDBiEOG091G0WP8
PhylomeDBiP17931
TreeFamiTF315551

Family and domain databases

CDDicd00070 GLECT, 1 hit
InterProiView protein in InterPro
IPR013320 ConA-like_dom_sf
IPR015534 Galectin_3
IPR001079 Galectin_CRD
PANTHERiPTHR11346:SF26 PTHR11346:SF26, 1 hit
PfamiView protein in Pfam
PF00337 Gal-bind_lectin, 1 hit
SMARTiView protein in SMART
SM00908 Gal-bind_lectin, 1 hit
SM00276 GLECT, 1 hit
SUPFAMiSSF49899 SSF49899, 1 hit
PROSITEiView protein in PROSITE
PS51304 GALECTIN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17931-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADNFSLHDA LSGSGNPNPQ GWPGAWGNQP AGAGGYPGAS YPGAYPGQAP
60 70 80 90 100
PGAYPGQAPP GAYPGAPGAY PGAPAPGVYP GPPSGPGAYP SSGQPSATGA
110 120 130 140 150
YPATGPYGAP AGPLIVPYNL PLPGGVVPRM LITILGTVKP NANRIALDFQ
160 170 180 190 200
RGNDVAFHFN PRFNENNRRV IVCNTKLDNN WGREERQSVF PFESGKPFKI
210 220 230 240 250
QVLVEPDHFK VAVNDAHLLQ YNHRVKKLNE ISKLGISGDI DLTSASYTMI
Length:250
Mass (Da):26,152
Last modified:November 25, 2008 - v5
Checksum:iC49DDF6D67AE0C88
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti33 – 52AGGYP…QAPPG → QGLPRGFLSWGLPRAGTPR (PubMed:2402511).CuratedAdd BLAST20
Sequence conflicti88Missing (PubMed:2402511).Curated1
Sequence conflicti232S → R in M64303 (PubMed:2009535).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01298864P → H3 PublicationsCorresponds to variant dbSNP:rs4644Ensembl.1
Natural variantiVAR_01298998T → P4 PublicationsCorresponds to variant dbSNP:rs4652Ensembl.1
Natural variantiVAR_049768183R → K. Corresponds to variant dbSNP:rs10148371Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57710 mRNA Translation: AAA35607.1
M35368 mRNA Translation: AAA88086.1
M36682 mRNA Translation: AAA36163.1
M64303 mRNA No translation available.
S59012 mRNA Translation: AAB26229.1
AF031425
, AF031422, AF031423, AF031424 Genomic DNA Translation: AAB86584.1
AB006780 mRNA Translation: BAA22164.1
AK314929 mRNA Translation: BAG37435.1
CR456897 mRNA Translation: CAG33178.1
AL139316 Genomic DNA No translation available.
CH471061 Genomic DNA Translation: EAW80658.1
BC001120 mRNA Translation: AAH01120.1
BC053667 mRNA Translation: AAH53667.1
CCDSiCCDS41956.1
PIRiA35820
RefSeqiNP_002297.2, NM_002306.3
UniGeneiHs.531081

Genome annotation databases

EnsembliENST00000254301; ENSP00000254301; ENSG00000131981
GeneIDi3958
KEGGihsa:3958
UCSCiuc001xbr.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiLEG3_HUMAN
AccessioniPrimary (citable) accession number: P17931
Secondary accession number(s): B2RC38
, Q16005, Q6IBA7, Q96J47
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 25, 2008
Last modified: April 25, 2018
This is version 210 of the entry and version 5 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health