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Protein

Galectin-3

Gene

LGALS3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.By similarity3 Publications

GO - Molecular functioni

  • carbohydrate binding Source: ProtInc
  • chemoattractant activity Source: BHF-UCL
  • IgE binding Source: BHF-UCL
  • laminin binding Source: BHF-UCL
  • poly(A) RNA binding Source: UniProtKB

GO - Biological processi

  • eosinophil chemotaxis Source: BHF-UCL
  • epithelial cell differentiation Source: UniProtKB
  • innate immune response Source: UniProtKB-KW
  • macrophage chemotaxis Source: BHF-UCL
  • monocyte chemotaxis Source: BHF-UCL
  • mononuclear cell migration Source: BHF-UCL
  • mRNA processing Source: UniProtKB-KW
  • negative regulation of endocytosis Source: BHF-UCL
  • negative regulation of extrinsic apoptotic signaling pathway Source: BHF-UCL
  • negative regulation of immunological synapse formation Source: BHF-UCL
  • negative regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell Source: BHF-UCL
  • negative regulation of T cell receptor signaling pathway Source: BHF-UCL
  • neutrophil chemotaxis Source: BHF-UCL
  • positive chemotaxis Source: BHF-UCL
  • positive regulation of calcium ion import Source: BHF-UCL
  • positive regulation of mononuclear cell migration Source: BHF-UCL
  • regulation of extrinsic apoptotic signaling pathway via death domain receptors Source: BHF-UCL
  • regulation of T cell apoptotic process Source: BHF-UCL
  • regulation of T cell proliferation Source: BHF-UCL
  • RNA splicing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Differentiation, Immunity, Innate immunity, mRNA processing, mRNA splicing

Keywords - Ligandi

IgE-binding protein, Lectin

Enzyme and pathway databases

ReactomeiR-HSA-879415. Advanced glycosylation endproduct receptor signaling.
SIGNORiP17931.

Names & Taxonomyi

Protein namesi
Recommended name:
Galectin-3
Short name:
Gal-3
Alternative name(s):
35 kDa lectin
Carbohydrate-binding protein 35
Short name:
CBP 35
Galactose-specific lectin 3
Galactoside-binding protein
Short name:
GALBP
IgE-binding protein
L-31
Laminin-binding protein
Lectin L-29
Mac-2 antigen
Gene namesi
Name:LGALS3
Synonyms:MAC2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:6563. LGALS3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: BHF-UCL
  • extracellular exosome Source: UniProtKB
  • extracellular space Source: UniProtKB
  • immunological synapse Source: BHF-UCL
  • membrane Source: UniProtKB
  • mitochondrial inner membrane Source: UniProtKB
  • nucleus Source: BHF-UCL
  • plasma membrane Source: ProtInc
  • spliceosomal complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Secreted, Spliceosome

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA30340.

Chemistry

ChEMBLiCHEMBL4531.

Polymorphism and mutation databases

BioMutaiLGALS3.
DMDMi215274262.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 250249Galectin-3PRO_0000076930Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei6 – 61Phosphoserine1 Publication
Modified residuei12 – 121Phosphoserine1 Publication
Disulfide bondi173 – 173InterchainBy similarity
Modified residuei188 – 1881PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Disulfide bond, Phosphoprotein

Proteomic databases

EPDiP17931.
MaxQBiP17931.
PaxDbiP17931.
PeptideAtlasiP17931.
PRIDEiP17931.

2D gel databases

DOSAC-COBS-2DPAGEP17931.
REPRODUCTION-2DPAGEIPI00465431.
UCD-2DPAGEP17931.

PTM databases

iPTMnetiP17931.
PhosphoSiteiP17931.

Miscellaneous databases

PMAP-CutDBQ6IBA7.

Expressioni

Tissue specificityi

A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Expressed in fetal membranes.1 Publication

Gene expression databases

BgeeiENSG00000131981.
CleanExiHS_LGALS3.
ExpressionAtlasiP17931. baseline and differential.
GenevisibleiP17931. HS.

Organism-specific databases

HPAiCAB005191.
HPA003162.

Interactioni

Subunit structurei

Probably forms homo- or heterodimers. Interacts with DMBT1 (By similarity). Interacts with CD6 and ALCAM (PubMed:24945728). Forms a complex with the ITGA3, ITGB1 and CSPG4. Interacts with LGALS3BP, LYPD3, CYHR1 and UACA.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AHSGP127632EBI-1170392,EBI-9396660From a different organism.
ALCAMQ137403EBI-1170392,EBI-1188108
CD6P302032EBI-1170392,EBI-2873748
GOLGA2Q083793EBI-1170392,EBI-618309
MICAQ299832EBI-1170392,EBI-1031130
PPIGQ134273EBI-1170392,EBI-396072
PRR13Q9NZ813EBI-1170392,EBI-740924
SS18L1O751773EBI-1170392,EBI-744674

GO - Molecular functioni

  • IgE binding Source: BHF-UCL
  • laminin binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi110149. 173 interactions.
DIPiDIP-45623N.
IntActiP17931. 51 interactions.
STRINGi9606.ENSP00000254301.

Chemistry

BindingDBiP17931.

Structurei

Secondary structure

1
250
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi116 – 1216Combined sources
Beta strandi130 – 1389Combined sources
Beta strandi144 – 1518Combined sources
Beta strandi154 – 16512Combined sources
Beta strandi168 – 17710Combined sources
Beta strandi185 – 1873Combined sources
Beta strandi197 – 2048Combined sources
Beta strandi206 – 2138Combined sources
Beta strandi216 – 2227Combined sources
Helixi228 – 2303Combined sources
Beta strandi233 – 24917Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1A3KX-ray2.10A114-250[»]
1KJLX-ray1.40A105-250[»]
1KJRX-ray1.55A105-250[»]
2NMNX-ray2.45A113-250[»]
2NMOX-ray1.35A113-250[»]
2NN8X-ray1.35A113-250[»]
2XG3X-ray1.20A114-250[»]
3AYAX-ray2.00A/B117-250[»]
3AYCX-ray1.80A/B117-250[»]
3AYDX-ray1.90A117-250[»]
3AYEX-ray2.00A/B117-250[»]
3T1LX-ray1.60A108-250[»]
3T1MX-ray1.55A108-250[»]
3ZSJX-ray0.86A113-250[»]
3ZSKX-ray0.90A114-250[»]
3ZSLX-ray1.08A114-250[»]
3ZSMX-ray1.25A114-250[»]
4BLIX-ray1.08A114-250[»]
4BLJX-ray1.20A114-250[»]
4BM8X-ray0.96A114-250[»]
4JC1X-ray1.50A108-250[»]
4JCKX-ray1.15A108-250[»]
4LBJX-ray1.80A114-250[»]
4LBKX-ray1.60A114-250[»]
4LBLX-ray1.58A114-250[»]
4LBMX-ray1.55A112-250[»]
4LBNX-ray1.70A112-250[»]
4LBOX-ray1.65A113-250[»]
4R9AX-ray1.20A111-250[»]
4R9BX-ray1.20A111-250[»]
4R9CX-ray1.19A111-250[»]
4R9DX-ray1.24A111-250[»]
4RL7X-ray2.00A111-250[»]
4XBNX-ray2.21A113-250[»]
ProteinModelPortaliP17931.
SMRiP17931. Positions 113-250.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP17931.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati36 – 4491
Repeati45 – 5392
Repeati54 – 6293
Repeati63 – 6974; approximate
Repeati70 – 7895
Repeati79 – 88106; approximate
Repeati89 – 100127; approximateAdd
BLAST
Repeati101 – 10998; approximate
Domaini118 – 248131GalectinPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni36 – 109748 X 9 AA tandem repeats of Y-P-G-X(3)-P-G-AAdd
BLAST
Regioni181 – 1877Beta-galactoside bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi226 – 24116Nuclear export signalBy similarityAdd
BLAST

Sequence similaritiesi

Contains 1 galectin domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3587. Eukaryota.
ENOG4111EA0. LUCA.
GeneTreeiENSGT00760000119105.
HOVERGENiHBG006255.
InParanoidiP17931.
KOiK06831.
OMAiWGKEERH.
OrthoDBiEOG091G0WP8.
PhylomeDBiP17931.
TreeFamiTF315551.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR001079. Galectin_CRD.
[Graphical view]
PfamiPF00337. Gal-bind_lectin. 1 hit.
[Graphical view]
SMARTiSM00908. Gal-bind_lectin. 1 hit.
SM00276. GLECT. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS51304. GALECTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17931-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADNFSLHDA LSGSGNPNPQ GWPGAWGNQP AGAGGYPGAS YPGAYPGQAP
60 70 80 90 100
PGAYPGQAPP GAYPGAPGAY PGAPAPGVYP GPPSGPGAYP SSGQPSATGA
110 120 130 140 150
YPATGPYGAP AGPLIVPYNL PLPGGVVPRM LITILGTVKP NANRIALDFQ
160 170 180 190 200
RGNDVAFHFN PRFNENNRRV IVCNTKLDNN WGREERQSVF PFESGKPFKI
210 220 230 240 250
QVLVEPDHFK VAVNDAHLLQ YNHRVKKLNE ISKLGISGDI DLTSASYTMI
Length:250
Mass (Da):26,152
Last modified:November 25, 2008 - v5
Checksum:iC49DDF6D67AE0C88
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti33 – 5220AGGYP…QAPPG → QGLPRGFLSWGLPRAGTPR (PubMed:2402511).CuratedAdd
BLAST
Sequence conflicti88 – 881Missing (PubMed:2402511).Curated
Sequence conflicti232 – 2321S → R in M64303 (PubMed:2009535).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti64 – 641P → H.3 Publications
Corresponds to variant rs4644 [ dbSNP | Ensembl ].
VAR_012988
Natural varianti98 – 981T → P.4 Publications
Corresponds to variant rs4652 [ dbSNP | Ensembl ].
VAR_012989
Natural varianti183 – 1831R → K.
Corresponds to variant rs10148371 [ dbSNP | Ensembl ].
VAR_049768

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57710 mRNA. Translation: AAA35607.1.
M35368 mRNA. Translation: AAA88086.1.
M36682 mRNA. Translation: AAA36163.1.
M64303 mRNA. No translation available.
S59012 mRNA. Translation: AAB26229.1.
AF031425
, AF031422, AF031423, AF031424 Genomic DNA. Translation: AAB86584.1.
AB006780 mRNA. Translation: BAA22164.1.
AK314929 mRNA. Translation: BAG37435.1.
CR456897 mRNA. Translation: CAG33178.1.
AL139316 Genomic DNA. No translation available.
CH471061 Genomic DNA. Translation: EAW80658.1.
BC001120 mRNA. Translation: AAH01120.1.
BC053667 mRNA. Translation: AAH53667.1.
CCDSiCCDS41956.1.
PIRiA35820.
RefSeqiNP_002297.2. NM_002306.3.
XP_011535061.2. XM_011536759.2.
UniGeneiHs.531081.

Genome annotation databases

EnsembliENST00000254301; ENSP00000254301; ENSG00000131981.
GeneIDi3958.
KEGGihsa:3958.
UCSCiuc001xbr.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - Glycan Binding

Galectin-3

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57710 mRNA. Translation: AAA35607.1.
M35368 mRNA. Translation: AAA88086.1.
M36682 mRNA. Translation: AAA36163.1.
M64303 mRNA. No translation available.
S59012 mRNA. Translation: AAB26229.1.
AF031425
, AF031422, AF031423, AF031424 Genomic DNA. Translation: AAB86584.1.
AB006780 mRNA. Translation: BAA22164.1.
AK314929 mRNA. Translation: BAG37435.1.
CR456897 mRNA. Translation: CAG33178.1.
AL139316 Genomic DNA. No translation available.
CH471061 Genomic DNA. Translation: EAW80658.1.
BC001120 mRNA. Translation: AAH01120.1.
BC053667 mRNA. Translation: AAH53667.1.
CCDSiCCDS41956.1.
PIRiA35820.
RefSeqiNP_002297.2. NM_002306.3.
XP_011535061.2. XM_011536759.2.
UniGeneiHs.531081.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1A3KX-ray2.10A114-250[»]
1KJLX-ray1.40A105-250[»]
1KJRX-ray1.55A105-250[»]
2NMNX-ray2.45A113-250[»]
2NMOX-ray1.35A113-250[»]
2NN8X-ray1.35A113-250[»]
2XG3X-ray1.20A114-250[»]
3AYAX-ray2.00A/B117-250[»]
3AYCX-ray1.80A/B117-250[»]
3AYDX-ray1.90A117-250[»]
3AYEX-ray2.00A/B117-250[»]
3T1LX-ray1.60A108-250[»]
3T1MX-ray1.55A108-250[»]
3ZSJX-ray0.86A113-250[»]
3ZSKX-ray0.90A114-250[»]
3ZSLX-ray1.08A114-250[»]
3ZSMX-ray1.25A114-250[»]
4BLIX-ray1.08A114-250[»]
4BLJX-ray1.20A114-250[»]
4BM8X-ray0.96A114-250[»]
4JC1X-ray1.50A108-250[»]
4JCKX-ray1.15A108-250[»]
4LBJX-ray1.80A114-250[»]
4LBKX-ray1.60A114-250[»]
4LBLX-ray1.58A114-250[»]
4LBMX-ray1.55A112-250[»]
4LBNX-ray1.70A112-250[»]
4LBOX-ray1.65A113-250[»]
4R9AX-ray1.20A111-250[»]
4R9BX-ray1.20A111-250[»]
4R9CX-ray1.19A111-250[»]
4R9DX-ray1.24A111-250[»]
4RL7X-ray2.00A111-250[»]
4XBNX-ray2.21A113-250[»]
ProteinModelPortaliP17931.
SMRiP17931. Positions 113-250.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110149. 173 interactions.
DIPiDIP-45623N.
IntActiP17931. 51 interactions.
STRINGi9606.ENSP00000254301.

Chemistry

BindingDBiP17931.
ChEMBLiCHEMBL4531.

PTM databases

iPTMnetiP17931.
PhosphoSiteiP17931.

Polymorphism and mutation databases

BioMutaiLGALS3.
DMDMi215274262.

2D gel databases

DOSAC-COBS-2DPAGEP17931.
REPRODUCTION-2DPAGEIPI00465431.
UCD-2DPAGEP17931.

Proteomic databases

EPDiP17931.
MaxQBiP17931.
PaxDbiP17931.
PeptideAtlasiP17931.
PRIDEiP17931.

Protocols and materials databases

DNASUi3958.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000254301; ENSP00000254301; ENSG00000131981.
GeneIDi3958.
KEGGihsa:3958.
UCSCiuc001xbr.4. human.

Organism-specific databases

CTDi3958.
GeneCardsiLGALS3.
HGNCiHGNC:6563. LGALS3.
HPAiCAB005191.
HPA003162.
MIMi153619. gene.
neXtProtiNX_P17931.
PharmGKBiPA30340.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3587. Eukaryota.
ENOG4111EA0. LUCA.
GeneTreeiENSGT00760000119105.
HOVERGENiHBG006255.
InParanoidiP17931.
KOiK06831.
OMAiWGKEERH.
OrthoDBiEOG091G0WP8.
PhylomeDBiP17931.
TreeFamiTF315551.

Enzyme and pathway databases

ReactomeiR-HSA-879415. Advanced glycosylation endproduct receptor signaling.
SIGNORiP17931.

Miscellaneous databases

ChiTaRSiLGALS3. human.
EvolutionaryTraceiP17931.
GeneWikiiLGALS3.
GenomeRNAii3958.
PMAP-CutDBQ6IBA7.
PROiP17931.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000131981.
CleanExiHS_LGALS3.
ExpressionAtlasiP17931. baseline and differential.
GenevisibleiP17931. HS.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR001079. Galectin_CRD.
[Graphical view]
PfamiPF00337. Gal-bind_lectin. 1 hit.
[Graphical view]
SMARTiSM00908. Gal-bind_lectin. 1 hit.
SM00276. GLECT. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS51304. GALECTIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLEG3_HUMAN
AccessioniPrimary (citable) accession number: P17931
Secondary accession number(s): B2RC38
, Q16005, Q6IBA7, Q96J47
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 25, 2008
Last modified: September 7, 2016
This is version 194 of the entry and version 5 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.