Reviewed,
UniProtKB/Swiss-Prot P17921 (SYFA_BACSU)
Last modified
November 3, 2009.
Version 84.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Phenylalanyl-tRNA synthetase alpha chain EC=6.1.1.20 Alternative name(s): Phenylalanine--tRNA ligase alpha chain Short name=PheRS | ||||
| Gene names |
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| Organism | Bacillus subtilis [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 1423 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus |
Protein attributes
| Sequence length | 344 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Catalytic activity | ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281 |
| Cofactor | Binds 2 magnesium ions per tetramer By similarity. |
| Subunit structure | Tetramer of two alpha and two beta chains By similarity. |
| Subcellular location | |
| Sequence similarities | Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Protein biosynthesis |
| Cellular component | Cytoplasm |
| Ligand | ATP-binding Magnesium Metal-binding Nucleotide-binding |
| Molecular function | Aminoacyl-tRNA synthetase Ligase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion bindingInferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activityInferred from electronic annotation. Source: HAMAP |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 344 | 344 | Phenylalanyl-tRNA synthetase alpha chain HAMAP MF_00281 | PRO_0000126666 | |||||
Sites | |||||||||
| Metal binding | 256 | 1 | Magnesium By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 90 – 105 | 16 | GQTID…PVAVG → DRQLTSRCREPCCSR in CAA37224. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Structure and nucleotide sequence of the Bacillus subtilis phenylalanyl-tRNA synthetase genes." Brakhage A., Wozny M., Putzer H. Biochimie 72:725-734(1990) [PubMed: 2127701] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168. |
| [2] | Erratum Brakhage A., Wozny M., Putzer H. Biochimie 73:127-127(1991) [PubMed: 1903307] [Abstract] |
| [3] | "The dnaB-pheA (256 degrees-240 degrees) region of the Bacillus subtilis chromosome containing genes responsible for stress responses, the utilization of plant cell walls and primary metabolism." Wipat A., Carter N., Brignell C.S., Guy J.B., Piper K., Sanders J., Emmerson P.T., Harwood C.R. Microbiology 142:3067-3078(1996) [PubMed: 8969504] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168. |
| [4] | "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. Danchin A.Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168. |
Cross-references
Sequence databases | |
|---|---|
| X53057 Genomic DNA. Translation: CAA37224.1. Z75208 Genomic DNA. Translation: CAA99603.1. AL009126 Genomic DNA. Translation: CAB14824.1. | |
| PIR | YFBSA. H69675. |
| RefSeq | NP_390742.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1JJC based on UniProtKB P27001. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 937987. |
| GenomeReviews | Gene locus BSU28640 in contig AL009126_GR. |
| KEGG | bsu:BSU28640. |
| NMPDR | fig|224308.1.peg.2867. |
Organism-specific databases | |
| SubtiList | BG10874. pheS. [Micado] |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | P17921. |
| OMA | FRASYFP. |
Enzyme and pathway databases | |
| BioCyc | BSUB224308:BSU2860-MON. |
| BRENDA | 6.1.1.20. 150. |
Family and domain databases | |
| HAMAP | MF_00281. [Tree] |
| InterPro | IPR006195. aa-tRNA-synth_II_cons-reg. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR002319. Phenylalanyl-tRNA_Synthase_acu. [Graphical view] |
| PANTHER | PTHR11538. tRNA-synt_2d. 1 hit. |
| Pfam | PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR00468. pheS. 1 hit. |
| PROSITE | PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | SYFA_BACSU | ||||||||
| Accession | Primary (citable) accession number: P17921 Secondary accession number(s): P94539 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Aminoacyl-tRNA synthetases List of aminoacyl-tRNA synthetase entries |
| Bacillus subtilis Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList |
| SIMILARITY comments Index of protein domains and families |

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