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Protein

Proliferating cell nuclear antigen

Gene

PCNA

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi61 – 8020Sequence analysisAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • antimicrobial humoral response Source: FlyBase
  • DNA-dependent DNA replication Source: FlyBase
  • DNA replication Source: FlyBase
  • eggshell chorion gene amplification Source: FlyBase
  • leading strand elongation Source: GO_Central
  • mismatch repair Source: FlyBase
  • mitotic spindle organization Source: FlyBase
  • neurogenesis Source: FlyBase
  • nucleotide-excision repair Source: FlyBase
  • regulation of DNA replication Source: InterPro
  • translesion synthesis Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-DME-110312. Translesion synthesis by REV1.
R-DME-110314. Recognition of DNA damage by PCNA-containing replication complex.
R-DME-110320. Translesion Synthesis by POLH.
R-DME-113510. E2F mediated regulation of DNA replication.
R-DME-1538133. G0 and Early G1.
R-DME-174411. Polymerase switching on the C-strand of the telomere.
R-DME-5358565. Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha).
R-DME-5651801. PCNA-Dependent Long Patch Base Excision Repair.
R-DME-5655862. Translesion synthesis by POLK.
R-DME-5656121. Translesion synthesis by POLI.
R-DME-5656169. Termination of translesion DNA synthesis.
R-DME-69091. Polymerase switching.
R-DME-69166. Removal of the Flap Intermediate.
R-DME-69183. Processive synthesis on the lagging strand.
R-DME-69205. G1/S-Specific Transcription.
SignaLinkiP17917.

Names & Taxonomyi

Protein namesi
Recommended name:
Proliferating cell nuclear antigen
Short name:
PCNA
Alternative name(s):
Cyclin
Mutagen-sensitive 209 protein
Gene namesi
Name:PCNA
Synonyms:mus209
ORF Names:CG9193
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0005655. PCNA.

Subcellular locationi

  • Nucleus 1 Publication

  • Note: Colocalizes with crm in polytene nuclei during embryogenesis.

GO - Cellular componenti

  • microtubule associated complex Source: FlyBase
  • nucleus Source: GO_Central
  • PCNA complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mutant flies show temperature-sensitive lethality, hypersensitivity to DNA-damaging agents such as ionizing radiation and methyl methanesulfonate, suppression of position-effect variegation and female sterility.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 260260Proliferating cell nuclear antigenPRO_0000149170Add
BLAST

Proteomic databases

PaxDbiP17917.
PRIDEiP17917.

Expressioni

Tissue specificityi

Expressed at high levels in adult ovary.1 Publication

Developmental stagei

Expressed maternally and zygotically. Expressed at high levels in unfertilized eggs and during early stages of embryogenesis. Low expression detected during late embryogenesis, in second and third instar larvae and in adult flies.1 Publication

Gene expression databases

BgeeiP17917.
ExpressionAtlasiP17917. differential.
GenevisibleiP17917. DM.

Interactioni

Subunit structurei

Homotrimer. Forms a complex with activator 1 heteropentamer in the presence of ATP (By similarity). Interacts with E2f.By similarity1 Publication

Protein-protein interaction databases

BioGridi62946. 70 interactions.
DIPiDIP-20560N.
IntActiP17917. 16 interactions.
MINTiMINT-833658.
STRINGi7227.FBpp0085619.

Structurei

Secondary structure

1
260
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 76Combined sources
Helixi9 – 1911Combined sources
Turni20 – 223Combined sources
Beta strandi24 – 318Combined sources
Beta strandi34 – 407Combined sources
Beta strandi44 – 5310Combined sources
Helixi54 – 563Combined sources
Beta strandi57 – 648Combined sources
Beta strandi66 – 716Combined sources
Helixi72 – 798Combined sources
Beta strandi87 – 926Combined sources
Beta strandi98 – 1047Combined sources
Beta strandi111 – 1177Combined sources
Beta strandi137 – 1404Combined sources
Helixi141 – 15111Combined sources
Turni152 – 1543Combined sources
Beta strandi156 – 1627Combined sources
Beta strandi167 – 1737Combined sources
Beta strandi176 – 1827Combined sources
Beta strandi203 – 2086Combined sources
Helixi209 – 2157Combined sources
Helixi216 – 2216Combined sources
Beta strandi223 – 2286Combined sources
Beta strandi235 – 2417Combined sources
Turni242 – 2443Combined sources
Beta strandi245 – 2517Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4HK1X-ray2.00A1-260[»]
ProteinModelPortaliP17917.
SMRiP17917. Positions 1-254.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PCNA family.Curated

Phylogenomic databases

eggNOGiKOG1636. Eukaryota.
COG0592. LUCA.
GeneTreeiENSGT00390000004965.
InParanoidiP17917.
KOiK04802.
OMAiSDGFDKY.
OrthoDBiEOG7JX34W.
PhylomeDBiP17917.

Family and domain databases

HAMAPiMF_00317. DNApol_clamp_arch.
InterProiIPR000730. Pr_cel_nuc_antig.
IPR022649. Pr_cel_nuc_antig_C.
IPR022659. Pr_cel_nuc_antig_CS.
IPR022648. Pr_cel_nuc_antig_N.
[Graphical view]
PANTHERiPTHR11352. PTHR11352. 1 hit.
PfamiPF02747. PCNA_C. 1 hit.
PF00705. PCNA_N. 1 hit.
[Graphical view]
PRINTSiPR00339. PCNACYCLIN.
TIGRFAMsiTIGR00590. pcna. 1 hit.
PROSITEiPS01251. PCNA_1. 1 hit.
PS00293. PCNA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P17917-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFEARLGQAT ILKKILDAIK DLLNEATFDC SDSGIQLQAM DNSHVSLVSL
60 70 80 90 100
TLRSDGFDKF RCDRNLSMGM NLGSMAKILK CANNEDNVTM KAQDNADTVT
110 120 130 140 150
IMFESANQEK VSDYEMKLMN LDQEHLGIPE TDFSCVVRMP AMEFARICRD
160 170 180 190 200
LAQFSESVVI CCTKEGVKFS ASGDVGTANI KLAQTGSVDK EEEAVIIEMQ
210 220 230 240 250
EPVTLTFACR YLNAFTKATP LSTQVQLSMC ADVPLVVEYA IKDLGHIRYY
260
LAPKIEDNET
Length:260
Mass (Da):28,830
Last modified:February 1, 1991 - v2
Checksum:i9A46280EA2C61FC5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33950 Genomic DNA. Translation: AAA28746.1.
AE013599 Genomic DNA. Translation: AAF57493.1.
AE013599 Genomic DNA. Translation: AAS64796.1.
AY122197 mRNA. Translation: AAM52709.1.
PIRiA34752.
RefSeqiNP_476905.1. NM_057557.4.
NP_995904.1. NM_206182.2.
UniGeneiDm.4751.

Genome annotation databases

EnsemblMetazoaiFBtr0086307; FBpp0085619; FBgn0005655.
FBtr0086308; FBpp0089395; FBgn0005655.
GeneIDi37290.
KEGGidme:Dmel_CG9193.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33950 Genomic DNA. Translation: AAA28746.1.
AE013599 Genomic DNA. Translation: AAF57493.1.
AE013599 Genomic DNA. Translation: AAS64796.1.
AY122197 mRNA. Translation: AAM52709.1.
PIRiA34752.
RefSeqiNP_476905.1. NM_057557.4.
NP_995904.1. NM_206182.2.
UniGeneiDm.4751.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4HK1X-ray2.00A1-260[»]
ProteinModelPortaliP17917.
SMRiP17917. Positions 1-254.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi62946. 70 interactions.
DIPiDIP-20560N.
IntActiP17917. 16 interactions.
MINTiMINT-833658.
STRINGi7227.FBpp0085619.

Proteomic databases

PaxDbiP17917.
PRIDEiP17917.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0086307; FBpp0085619; FBgn0005655.
FBtr0086308; FBpp0089395; FBgn0005655.
GeneIDi37290.
KEGGidme:Dmel_CG9193.

Organism-specific databases

CTDi5111.
FlyBaseiFBgn0005655. PCNA.

Phylogenomic databases

eggNOGiKOG1636. Eukaryota.
COG0592. LUCA.
GeneTreeiENSGT00390000004965.
InParanoidiP17917.
KOiK04802.
OMAiSDGFDKY.
OrthoDBiEOG7JX34W.
PhylomeDBiP17917.

Enzyme and pathway databases

ReactomeiR-DME-110312. Translesion synthesis by REV1.
R-DME-110314. Recognition of DNA damage by PCNA-containing replication complex.
R-DME-110320. Translesion Synthesis by POLH.
R-DME-113510. E2F mediated regulation of DNA replication.
R-DME-1538133. G0 and Early G1.
R-DME-174411. Polymerase switching on the C-strand of the telomere.
R-DME-5358565. Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha).
R-DME-5651801. PCNA-Dependent Long Patch Base Excision Repair.
R-DME-5655862. Translesion synthesis by POLK.
R-DME-5656121. Translesion synthesis by POLI.
R-DME-5656169. Termination of translesion DNA synthesis.
R-DME-69091. Polymerase switching.
R-DME-69166. Removal of the Flap Intermediate.
R-DME-69183. Processive synthesis on the lagging strand.
R-DME-69205. G1/S-Specific Transcription.
SignaLinkiP17917.

Miscellaneous databases

GenomeRNAii37290.
PROiP17917.

Gene expression databases

BgeeiP17917.
ExpressionAtlasiP17917. differential.
GenevisibleiP17917. DM.

Family and domain databases

HAMAPiMF_00317. DNApol_clamp_arch.
InterProiIPR000730. Pr_cel_nuc_antig.
IPR022649. Pr_cel_nuc_antig_C.
IPR022659. Pr_cel_nuc_antig_CS.
IPR022648. Pr_cel_nuc_antig_N.
[Graphical view]
PANTHERiPTHR11352. PTHR11352. 1 hit.
PfamiPF02747. PCNA_C. 1 hit.
PF00705. PCNA_N. 1 hit.
[Graphical view]
PRINTSiPR00339. PCNACYCLIN.
TIGRFAMsiTIGR00590. pcna. 1 hit.
PROSITEiPS01251. PCNA_1. 1 hit.
PS00293. PCNA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Drosophila proliferating cell nuclear antigen (cyclin) gene: structure, expression during development, and specific binding of homeodomain proteins to its 5'-flanking region."
    Yamaguchi M., Nishida Y., Moriuchi T., Hirose F., Hui C.C., Suzuki Y., Matsukage A.
    Mol. Cell. Biol. 10:872-879(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.
  5. "Drosophila proliferating cell nuclear antigen. Structural and functional homology with its mammalian counterpart."
    Ng L., Prelich G., Anderson C.W., Stillman B., Fisher P.A.
    J. Biol. Chem. 265:11948-11954(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-22.
    Strain: Oregon-R.
  6. "Mutagen sensitivity and suppression of position-effect variegation result from mutations in mus209, the Drosophila gene encoding PCNA."
    Henderson D.S., Banga S.S., Grigliatto T.A., Boyd J.B.
    EMBO J. 13:1450-1459(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  7. "The cramped gene of Drosophila is a member of the polycomb-group, and interacts with mus209, the gene encoding proliferating cell nuclear antigen."
    Yamamoto Y., Girard F., Bello B., Affolter M., Gehring W.J.
    Development 124:3385-3394(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: Oregon-R.
    Tissue: Embryo.
  8. "Intrinsic negative cell cycle regulation provided by PIP box- and Cul4Cdt2-mediated destruction of E2f1 during S phase."
    Shibutani S.T., de la Cruz A.F., Tran V., Turbyfill W.J. III, Reis T., Edgar B.A., Duronio R.J.
    Dev. Cell 15:890-900(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH E2F.

Entry informationi

Entry nameiPCNA_DROME
AccessioniPrimary (citable) accession number: P17917
Secondary accession number(s): Q540V1, Q9V909
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: February 1, 1991
Last modified: July 6, 2016
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.