P17906 (RSBX_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoserine phosphatase RsbX
EC=3.1.3.3

Alternative name(s):
Sigma-B negative effector

Gene names

Name:	rsbX
Ordered Locus Names:	BSU04740

Organism

Bacillus subtilis (strain 168) [Reference proteome] [HAMAP]

Taxonomic identifier

224308 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus subtilis group › Bacillus subtilis › Bacillus subtilis subsp. subtilis

Protein attributes

Sequence length	199 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Negative regulator of sigma-B activity. Dephosphorylates RsbS. Plays a role both in maintaining low sigma-B activity during growth and in reestablishing prestress sigma-B activity after induction. Could have a negative feedback role by indirectly communicating sigma-B protein levels. Ref.5 Ref.6 Ref.7 Ref.8
Catalytic activity	O-phospho-L(or D)-serine + H2O = L(or D)-serine + phosphate.
Sequence similarities	Contains 1 PP2C-like domain.

Ontologies

Keywords
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	metabolic process Inferred from electronic annotation. Source: GOC
Molecular_function	phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW phosphoserine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 199	199	Phosphoserine phosphatase RsbX		PRO_0000057791
Regions
	Domain	22 – 199	178	PP2C-like

Sequences

	Sequence		Length	Mass (Da)
	P17906 [UniParc]. Last modified November 1, 1990. Version 1. Checksum: 2AAEFB96FB072E33	FASTA	199	22,144
	10 20 30 40 50 60 MIQVEENEHI QTLVYQLNKE GKSICGDSFF MKADDKELIC AVADGLGSGS LANESSAAIK 70 80 90 100 110 120 DLVENYASED VESIIERCNQ AMKNKRGATA SILKINFEQR QFTYCSVGNV RFILHSPSGE 130 140 150 160 170 180 SFYPLPISGY LSGKPQKYKT HTATYEKGSK FIIHTDGLNV PDIRSHLKKG QSVEEISNSL 190 KMYTTSRKDD LTYILGQLS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Similar organization of the sigB and spoIIA operons encoding alternate sigma factors of Bacillus subtilis RNA polymerase." Kalman S., Duncan M.L., Thomas S.M., Price C.W. J. Bacteriol. 172:5575-5585(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"A 148 kbp sequence of the region between 35 and 47 degree of the Bacillus subtilis genome." Kasahara Y., Nakai S., Lee S., Sadaie Y., Ogasawara N. Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"Gene encoding the 37,000-dalton minor sigma factor of Bacillus subtilis RNA polymerase: isolation, nucleotide sequence, chromosomal locus, and cryptic function." Duncan M.L., Kalman S.S., Thomas S.M., Price C.W. J. Bacteriol. 169:771-778(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-179. Strain: 168.
[5]	"Activation of Bacillus subtilis transcription factor sigma B by a regulatory pathway responsive to stationary-phase signals." Boylan S.A., Rutherford A., Thomas S.M., Price C.W. J. Bacteriol. 174:3695-3706(1992) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
[6]	"Regulation of sigma B levels and activity in Bacillus subtilis." Benson A.K., Haldenwang W.G. J. Bacteriol. 175:2347-2356(1993) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. Strain: PY22.
[7]	"Opposing pairs of serine protein kinases and phosphatases transmit signals of environmental stress to activate a bacterial transcription factor." Yang X., Kang C.M., Brody M.S., Price C.W. Genes Dev. 10:2265-2275(1996) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
[8]	"Isolation and characterization of Bacillus subtilis sigB operon mutations that suppress the loss of the negative regulator RsbX." Smirnova N., Scott J., Voelker U., Haldenwang W.G. J. Bacteriol. 180:3671-3680(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. Strain: PY22.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M34995 Genomic DNA. Translation: AAA22714.1. AB001488 Genomic DNA. Translation: BAA19311.1. AL009126 Genomic DNA. Translation: CAB12281.1.
PIR	D36131.
RefSeq	NP_388355.1. NC_000964.3.
3D structure databases
ProteinModelPortal	P17906.
ModBase	Search...
Protein-protein interaction databases
STRING	224308.BSU04740.
Protein family/group databases
PptaseDB	P3D0406129.
Proteomic databases
PaxDb	P17906.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAB12281; CAB12281; BSU04740.
GeneID	938155.
KEGG	bsu:BSU04740.
PATRIC	18972532. VBIBacSub10457_0494.
Organism-specific databases
GenoList	BSU04740. [Micado]
Phylogenomic databases
eggNOG	NOG07987.
HOGENOM	HOG000270275.
KO	K05518.
OMA	MIGIEDK.
ProtClustDB	CLSK886747.
Enzyme and pathway databases
BioCyc	BSUB:BSU04740-MONOMER.
Family and domain databases
InterPro	IPR001932. PP2C-like. [Graphical view]
Pfam	PF07228. SpoIIE. 1 hit. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RSBX_BACSU

Accession

Primary (citable) accession number: P17906

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	May 1, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program