Reviewed, UniProtKB/Swiss-Prot P17906 (RSBX_BACSU)

Names and origin

Protein names

Recommended name:
    Phosphoserine phosphatase rsbX
    EC=3.1.3.3
Alternative name(s):
    Sigma-B negative effector

Gene names

Name:	rsbX
Ordered Locus Names:	BSU04740

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	199 AA.
Sequence status	Complete.
Protein existence	Predicted.

General annotation (Comments)

Function	Negative regulator of sigma-B activity. Dephosphorylates rsbS. Plays a role both in maintaining low sigma-B activity during growth and in reestablishing prestress sigma-B activity after induction. Could have a negative feedback role by indirectly communicating sigma-B protein levels. Ref.5 Ref.6 Ref.7 Ref.8
Catalytic activity	O-phospho-L(or D)-serine + H2O = L(or D)-serine + phosphate.
Sequence similarities	Contains 1 PP2C-like domain.

Ontologies

Keywords
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW phosphoserine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 199	199	Phosphoserine phosphatase rsbX		PRO_0000057791
Regions
	Domain	22 – 199	178	PP2C-like

Sequences

	Sequence		Length	Mass (Da)
	P17906-1 [UniParc]. Last modified November 1, 1990. Version 1. Checksum: 2AAEFB96FB072E33	FASTA	199	22,144
	10 20 30 40 50 60 MIQVEENEHI QTLVYQLNKE GKSICGDSFF MKADDKELIC AVADGLGSGS LANESSAAIK 70 80 90 100 110 120 DLVENYASED VESIIERCNQ AMKNKRGATA SILKINFEQR QFTYCSVGNV RFILHSPSGE 130 140 150 160 170 180 SFYPLPISGY LSGKPQKYKT HTATYEKGSK FIIHTDGLNV PDIRSHLKKG QSVEEISNSL 190 KMYTTSRKDD LTYILGQLS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Similar organization of the sigB and spoIIA operons encoding alternate sigma factors of Bacillus subtilis RNA polymerase." Kalman S., Duncan M.L., Thomas S.M., Price C.W. J. Bacteriol. 172:5575-5585(1990) [PubMed: 2170324] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"A 148 kbp sequence of the region between 35 and 47 degree of the Bacillus subtilis genome." Kasahara Y., Nakai S., Lee S., Sadaie Y., Ogasawara N. Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"Gene encoding the 37,000-dalton minor sigma factor of Bacillus subtilis RNA polymerase: isolation, nucleotide sequence, chromosomal locus, and cryptic function." Duncan M.L., Kalman S.S., Thomas S.M., Price C.W. J. Bacteriol. 169:771-778(1987) [PubMed: 3027048] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-179. Strain: 168.
[5]	"Activation of Bacillus subtilis transcription factor sigma B by a regulatory pathway responsive to stationary-phase signals." Boylan S.A., Rutherford A., Thomas S.M., Price C.W. J. Bacteriol. 174:3695-3706(1992) [PubMed: 1592822] [Abstract] Cited for: FUNCTION. Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NCIB 3610.
[6]	"Regulation of sigma B levels and activity in Bacillus subtilis." Benson A.K., Haldenwang W.G. J. Bacteriol. 175:2347-2356(1993) [PubMed: 8468294] [Abstract] Cited for: FUNCTION. Strain: PY22.
[7]	"Opposing pairs of serine protein kinases and phosphatases transmit signals of environmental stress to activate a bacterial transcription factor." Yang X., Kang C.M., Brody M.S., Price C.W. Genes Dev. 10:2265-2275(1996) [PubMed: 8824586] [Abstract] Cited for: FUNCTION. Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NCIB 3610.
[8]	"Isolation and characterization of Bacillus subtilis sigB operon mutations that suppress the loss of the negative regulator RsbX." Smirnova N., Scott J., Voelker U., Haldenwang W.G. J. Bacteriol. 180:3671-3680(1998) [PubMed: 9658013] [Abstract] Cited for: FUNCTION. Strain: PY22.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M34995 Genomic DNA. Translation: AAA22714.1. AB001488 Genomic DNA. Translation: BAA19311.1. AL009126 Genomic DNA. Translation: CAB12281.1.
PIR	D36131.
RefSeq	NP_388355.1.
3D structure databases
ModBase	Search...
Protein family/group databases
PptaseDB	P3D0406129.
Genome annotation databases
GeneID	938155.
GenomeReviews	Gene locus BSU04740 in contig AL009126_GR.
KEGG	bsu:BSU04740.
NMPDR	fig|224308.1.peg.474.
Organism-specific databases
SubtiList	BG10736. rsbX. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	HBG338562.
OMA	MIGIEDK.
Enzyme and pathway databases
BRENDA	3.1.3.3. 150.
Family and domain databases
InterPro	IPR001932. PP2C-related. IPR010822. Spore_II_E_C. [Graphical view]
Pfam	PF07228. SpoIIE. 1 hit. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RSBX_BACSU

Accession

Primary (citable) accession number: P17906

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	December 15, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)