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P17906 (RSBX_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoserine phosphatase RsbX

EC=3.1.3.3
Alternative name(s):
Sigma-B negative effector
Gene names
Name:rsbX
Ordered Locus Names:BSU04740
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length199 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Negative regulator of sigma-B activity. Dephosphorylates RsbS. Plays a role both in maintaining low sigma-B activity during growth and in reestablishing prestress sigma-B activity after induction. Could have a negative feedback role by indirectly communicating sigma-B protein levels. Ref.5 Ref.6 Ref.7 Ref.8

Catalytic activity

O-phospho-L(or D)-serine + H2O = L(or D)-serine + phosphate.

Sequence similarities

Contains 1 PP2C-like domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 199199Phosphoserine phosphatase RsbX
PRO_0000057791

Regions

Domain22 – 199178PP2C-like

Secondary structure

.................................. 199
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P17906 [UniParc].

Last modified November 1, 1990. Version 1.
Checksum: 2AAEFB96FB072E33

FASTA19922,144
        10         20         30         40         50         60 
MIQVEENEHI QTLVYQLNKE GKSICGDSFF MKADDKELIC AVADGLGSGS LANESSAAIK 

        70         80         90        100        110        120 
DLVENYASED VESIIERCNQ AMKNKRGATA SILKINFEQR QFTYCSVGNV RFILHSPSGE 

       130        140        150        160        170        180 
SFYPLPISGY LSGKPQKYKT HTATYEKGSK FIIHTDGLNV PDIRSHLKKG QSVEEISNSL 

       190 
KMYTTSRKDD LTYILGQLS 

« Hide

References

« Hide 'large scale' references
[1]"Similar organization of the sigB and spoIIA operons encoding alternate sigma factors of Bacillus subtilis RNA polymerase."
Kalman S., Duncan M.L., Thomas S.M., Price C.W.
J. Bacteriol. 172:5575-5585(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"A 148 kbp sequence of the region between 35 and 47 degree of the Bacillus subtilis genome."
Kasahara Y., Nakai S., Lee S., Sadaie Y., Ogasawara N.
Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[3]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[4]"Gene encoding the 37,000-dalton minor sigma factor of Bacillus subtilis RNA polymerase: isolation, nucleotide sequence, chromosomal locus, and cryptic function."
Duncan M.L., Kalman S.S., Thomas S.M., Price C.W.
J. Bacteriol. 169:771-778(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-179.
Strain: 168.
[5]"Activation of Bacillus subtilis transcription factor sigma B by a regulatory pathway responsive to stationary-phase signals."
Boylan S.A., Rutherford A., Thomas S.M., Price C.W.
J. Bacteriol. 174:3695-3706(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
[6]"Regulation of sigma B levels and activity in Bacillus subtilis."
Benson A.K., Haldenwang W.G.
J. Bacteriol. 175:2347-2356(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
Strain: PY22.
[7]"Opposing pairs of serine protein kinases and phosphatases transmit signals of environmental stress to activate a bacterial transcription factor."
Yang X., Kang C.M., Brody M.S., Price C.W.
Genes Dev. 10:2265-2275(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
[8]"Isolation and characterization of Bacillus subtilis sigB operon mutations that suppress the loss of the negative regulator RsbX."
Smirnova N., Scott J., Voelker U., Haldenwang W.G.
J. Bacteriol. 180:3671-3680(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
Strain: PY22.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M34995 Genomic DNA. Translation: AAA22714.1.
AB001488 Genomic DNA. Translation: BAA19311.1.
AL009126 Genomic DNA. Translation: CAB12281.1.
PIRD36131.
RefSeqNP_388355.1. NC_000964.3.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3W40X-ray1.30A/B1-199[»]
3W41X-ray1.42A1-199[»]
3W42X-ray1.06A/B1-199[»]
3W43X-ray1.22A1-199[»]
3W44X-ray2.30A/B1-199[»]
3W45X-ray1.70A/B1-199[»]
ProteinModelPortalP17906.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU04740.

Protein family/group databases

PptaseDBP3D0406129.

Proteomic databases

PaxDbP17906.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB12281; CAB12281; BSU04740.
GeneID938155.
KEGGbsu:BSU04740.
PATRIC18972532. VBIBacSub10457_0494.

Organism-specific databases

GenoListBSU04740. [Micado]

Phylogenomic databases

eggNOGNOG07987.
HOGENOMHOG000270275.
KOK05518.
OMARCNQAMK.
OrthoDBEOG6K401B.
PhylomeDBP17906.

Enzyme and pathway databases

BioCycBSUB:BSU04740-MONOMER.

Family and domain databases

InterProIPR001932. PP2C-like_dom.
[Graphical view]
PfamPF07228. SpoIIE. 1 hit.
[Graphical view]
SMARTSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMSSF81606. SSF81606. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRSBX_BACSU
AccessionPrimary (citable) accession number: P17906
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: July 9, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList