Cholinephosphotransferase 1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P17898 (CPT1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 111. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Cholinephosphotransferase 1
EC=2.7.8.2

Alternative name(s):
Diacylglycerol cholinephosphotransferase 1
Sn-1,2-diacylglycerol cholinephosphotransferase
Short name=CHOPT

Gene names

Name:	CPT1
Ordered Locus Names:	YNL130C
ORF Names:	N1218, N1867

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in protein-dependent process of phospholipid transport to distribute phosphatidyl choline to the lumenal surface. The multiple transmembrane domains and lumenal hydrophilic domains of the cholinephosphotransferase might participate in the transport process.
Catalytic activity	CDP-choline + 1,2-diacylglycerol = CMP + a phosphatidylcholine.
Cofactor	Phospholipid.
Enzyme regulation	Requires a divalent cation activator, and is inhibited by CMP.
Pathway	Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine from phosphocholine: step 2/2.
Subcellular location	Microsome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.7.
Miscellaneous	Present with 981 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the CDP-alcohol phosphatidyltransferase class-I family.
Sequence caution	The sequence AAA63571.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA86895.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA96012.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Lipid biosynthesis Lipid metabolism Phospholipid biosynthesis Phospholipid metabolism
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane Transmembrane helix
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	Golgi apparatus Inferred from direct assay PubMed 8543066. Source: SGD endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from sequence model PubMed 12192589 Ref.1. Source: SGD mitochondrial outer membrane Inferred from direct assay PubMed 3005242. Source: SGD
Molecular_function	diacylglycerol cholinephosphotransferase activity Inferred from mutant phenotype PubMed 3029130. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
itself		1	EBI-2050738,EBI-2050738
AGP1	P25376	1	EBI-2050738,EBI-2357
CHO1	P08456	1	EBI-2050738,EBI-14055
ERG11	P10614	1	EBI-2050738,EBI-5127
ERP1	Q05359	1	EBI-2050738,EBI-6581
ERV25	P54837	1	EBI-2050738,EBI-6642
GAP1	P19145	1	EBI-2050738,EBI-7314
OST1	P41543	1	EBI-2050738,EBI-12651
PHO84	P25297	1	EBI-2050738,EBI-13320
PMP2	P40975	1	EBI-2050738,EBI-2043041
PMP3	P87284	1	EBI-2050738,EBI-13555
PPZ1	P26570	1	EBI-2050738,EBI-13807
RBD2	Q12270	1	EBI-2050738,EBI-31471
RTN1	Q04947	1	EBI-2050738,EBI-38020
SAC1	P32368	1	EBI-2050738,EBI-16210
SEC63	P14906	1	EBI-2050738,EBI-16636
SPF1	P39986	1	EBI-2050738,EBI-3128
SWP1	Q02795	1	EBI-2050738,EBI-12666
UBP1	P25037	1	EBI-2050738,EBI-19819
VRG4	P40107	1	EBI-2050738,EBI-7764
YER053C-A	Q3E7B0	1	EBI-2050738,EBI-2047907

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 393

393

Cholinephosphotransferase 1

PRO_0000056808

Regions

Topological domain

1 – 40

Lumenal Potential

Transmembrane

41 – 61

Helical; Potential

Topological domain

62 – 172

111

Cytoplasmic Potential

Transmembrane

173 – 193

Helical; Potential

Topological domain

194 – 210

Lumenal Potential

Transmembrane

211 – 231

Helical; Potential

Topological domain

232 – 263

Cytoplasmic Potential

Transmembrane

264 – 284

Helical; Potential

Topological domain

285

Lumenal Potential

Transmembrane

286 – 306

Helical; Potential

Topological domain

307 – 320

Cytoplasmic Potential

Transmembrane

321 – 341

Helical; Potential

Topological domain

342 – 348

Lumenal Potential

Transmembrane

349 – 369

Helical; Potential

Topological domain

370 – 393

Cytoplasmic Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P17898 [UniParc].

Last modified September 5, 2006. Version 3.
Checksum: 320845FE9A79C5D6
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FASTA

393

44,829

        10         20         30         40         50         60 
MGFFIPQSSL GNLKLYKYQS DDRSFLSNHV LRPFWRKFAT IFPLWMAPNL VTLLGFCFII 

        70         80         90        100        110        120 
FNVLTTLYYD PYFDQESPRW TYFSYAIGLF LYQTFDACDG MHARRTGQQG PLGELFDHCI 

       130        140        150        160        170        180 
DSINTTLSMI PVCSMTGMGY TYMTIFSQFA ILCSFYLSTW EEYHTHKLYL AEFCGPVEGI 

       190        200        210        220        230        240 
IVLCISFIAV GIYGPQTIWH TKVAQFSWQD FVFDVETVHL MYAFCTGALI FNIVTAHTNV 

       250        260        270        280        290        300 
VRYYESQSTK SATPSKTAEN ISKAVNGLLP FFAYFSSIFT LVLIQPSFIS LALILSIGFS 

       310        320        330        340        350        360 
VAFVVGRMII AHLTMQPFPM VNFPFLIPTI QLVLYAFMVY VLDYQKGSIV SALVWMGLGL 

       370        380        390 
TLAIHGMFIN DIIYDITTFL DIYALSIKHP KEI

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sn-1,2-diacylglycerol cholinephosphotransferase of Saccharomyces cerevisiae. Nucleotide sequence, transcriptional mapping, and gene product analysis of the CPT1 gene." Hjelmstad R.H., Bell R.M. J. Biol. Chem. 265:1755-1764(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A 43.5 kb segment of yeast chromosome XIV, which contains MFA2, MEP2, CAP/SRV2, NAM9, FKB1/FPR1/RBP1, MOM22 and CPT1, predicts an adenosine deaminase gene and 14 new open reading frames." Mallet L., Bussereau F., Jacquet M. Yeast 11:1195-1209(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"sn-1,2-diacylglycerol choline- and ethanolaminephosphotransferases in Saccharomyces cerevisiae. Mixed micellar analysis of the CPT1 and EPT1 gene products." Hjelmstad R.H., Bell R.M. J. Biol. Chem. 266:4357-4365(1991) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[6]	"Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii." Brachat S., Dietrich F.S., Voegeli S., Zhang Z., Stuart L., Lerch A., Gates K., Gaffney T.D., Philippsen P. Genome Biol. 4:R45.1-R45.13(2003) [PubMed] [Europe PMC] [Abstract] Cited for: REVISION OF GENE MODEL.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J05203 Genomic DNA. Translation: AAA63571.1. Different initiation. Z46843 Genomic DNA. Translation: CAA86895.1. Different initiation. Z71406 Genomic DNA. Translation: CAA96012.1. Sequence problems. BK006947 Genomic DNA. Translation: DAA10418.1.
PIR	S63075.
RefSeq	NP_014269.4. NM_001182968.3.
3D structure databases
ProteinModelPortal	P17898.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-7848N.
IntAct	P17898. 7 interactions.
MINT	MINT-4499922.
STRING	4932.YNL130C.
Proteomic databases
PaxDb	P17898.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL130C; YNL130C; YNL130C.
GeneID	855593.
KEGG	sce:YNL124W. sce:YNL130C.
Organism-specific databases
CYGD	YNL130c.
SGD	S000005074. CPT1.
Phylogenomic databases
eggNOG	COG5050.
GeneTree	ENSGT00530000063048.
HOGENOM	HOG000157893.
KO	K00994. K14763.
OMA	GMFINDI.
OrthoDB	EOG4K9FN5.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-13671.
UniPathway	UPA00753; UER00740.
Gene expression databases
Genevestigator	P17898.
GermOnline	YNL130C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000462. CDP-OH_P_trans. IPR014472. CHOPT. [Graphical view]
Pfam	PF01066. CDP-OH_P_transf. 1 hit. [Graphical view]
PIRSF	PIRSF015665. CHOPT. 1 hit.
PROSITE	PS00379. CDP_ALCOHOL_P_TRANSF. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	979741.

Entry information

Entry name

CPT1_YEAST

Accession

Primary (citable) accession number: P17898
Secondary accession number(s): D6W152

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	September 5, 2006
Last modified:	May 1, 2013
This is version 111 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents