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Protein

Clathrin light chain

Gene

CLC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. In yeast, it is involved in the retention of proteins in an intracellular membrane compartment, presumably the trans-Golgi. The yeast light chain is important for cell growth. The light chain may help to properly orient the assembly/ disassembly of the clathrin coats.

GO - Molecular functioni

  • structural molecule activity Source: SGD

GO - Biological processi

  • endocytosis Source: SGD
  • intracellular protein transport Source: InterPro
  • positive regulation of endocytosis Source: SGD
  • vesicle-mediated transport Source: SGD
Complete GO annotation...

Keywords - Ligandi

Calcium, Calmodulin-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30865-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Clathrin light chain
Short name:
CLC
Gene namesi
Name:CLC1
Ordered Locus Names:YGR167W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome VII

Organism-specific databases

CYGDiYGR167w.
EuPathDBiFungiDB:YGR167W.
SGDiS000003399. CLC1.

Subcellular locationi

GO - Cellular componenti

  • clathrin coat of coated pit Source: InterPro
  • clathrin coat of trans-Golgi network vesicle Source: InterPro
  • clathrin vesicle coat Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Coated pit, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 233233Clathrin light chainPRO_0000205776Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei49 – 491Phosphothreonine1 Publication
Modified residuei52 – 521Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP17891.
PaxDbiP17891.
PeptideAtlasiP17891.

Interactioni

Subunit structurei

Clathrin coats are formed from molecules containing 3 heavy chains and 3 light chains. Interacts with the auxilin-like clathrin uncoating factor SWA2.1 Publication

Protein-protein interaction databases

BioGridi33419. 400 interactions.
DIPiDIP-2280N.
IntActiP17891. 12 interactions.
MINTiMINT-527297.
STRINGi4932.YGR167W.

Structurei

3D structure databases

ProteinModelPortaliP17891.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni144 – 20461Involved in binding clathrin heavy chainSequence AnalysisAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili125 – 18662Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the clathrin light chain family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG251359.
HOGENOMiHOG000190326.
InParanoidiP17891.
OMAiSTFESQF.
OrthoDBiEOG7Z95XB.

Family and domain databases

InterProiIPR000996. Clathrin_L-chain.
[Graphical view]
PANTHERiPTHR10639. PTHR10639. 1 hit.
PfamiPF01086. Clathrin_lg_ch. 1 hit.
[Graphical view]
PROSITEiPS00581. CLATHRIN_LIGHT_CHN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P17891-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEKFPPLED QNIDFTPNDK KDDDTDFLKR EAEILGDEFK TEQDDILETE
60 70 80 90 100
ASPAKDDDEI RDFEEQFPDI NSANGAVSSD QNGSATVSSG NDNGEADDDF
110 120 130 140 150
STFEGANQST ESVKEDRSEV VDQWKQRRAV EIHEKDLKDE ELKKELQDEA
160 170 180 190 200
IKHIDDFYDS YNKKKEQQLE DAAKEAEAFL KKRDEFFGQD NTTWDRALQL
210 220 230
INQDDADIIG GRDRSKLKEI LLRLKGNAKA PGA
Length:233
Mass (Da):26,532
Last modified:November 1, 1990 - v1
Checksum:i27BAB175780EC8B3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52272 Genomic DNA. Translation: CAA36515.1.
Z72952 Genomic DNA. Translation: CAA97192.1.
Z72953 Genomic DNA. Translation: CAA97193.1.
AY558272 Genomic DNA. Translation: AAS56598.1.
BK006941 Genomic DNA. Translation: DAA08261.1.
PIRiA36425.
RefSeqiNP_011683.3. NM_001181296.3.

Genome annotation databases

EnsemblFungiiYGR167W; YGR167W; YGR167W.
GeneIDi853077.
KEGGisce:YGR167W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52272 Genomic DNA. Translation: CAA36515.1.
Z72952 Genomic DNA. Translation: CAA97192.1.
Z72953 Genomic DNA. Translation: CAA97193.1.
AY558272 Genomic DNA. Translation: AAS56598.1.
BK006941 Genomic DNA. Translation: DAA08261.1.
PIRiA36425.
RefSeqiNP_011683.3. NM_001181296.3.

3D structure databases

ProteinModelPortaliP17891.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33419. 400 interactions.
DIPiDIP-2280N.
IntActiP17891. 12 interactions.
MINTiMINT-527297.
STRINGi4932.YGR167W.

Proteomic databases

MaxQBiP17891.
PaxDbiP17891.
PeptideAtlasiP17891.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR167W; YGR167W; YGR167W.
GeneIDi853077.
KEGGisce:YGR167W.

Organism-specific databases

CYGDiYGR167w.
EuPathDBiFungiDB:YGR167W.
SGDiS000003399. CLC1.

Phylogenomic databases

eggNOGiNOG251359.
HOGENOMiHOG000190326.
InParanoidiP17891.
OMAiSTFESQF.
OrthoDBiEOG7Z95XB.

Enzyme and pathway databases

BioCyciYEAST:G3O-30865-MONOMER.

Miscellaneous databases

NextBioi973038.
PROiP17891.

Family and domain databases

InterProiIPR000996. Clathrin_L-chain.
[Graphical view]
PANTHERiPTHR10639. PTHR10639. 1 hit.
PfamiPF01086. Clathrin_lg_ch. 1 hit.
[Graphical view]
PROSITEiPS00581. CLATHRIN_LIGHT_CHN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Yeast clathrin has a distinctive light chain that is important for cell growth."
    Silveira L.A., Wong D.H., Masiarz F.R., Schekman R.
    J. Cell Biol. 111:1437-1449(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 31-39.
  2. "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
    Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
    Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "The auxilin-like phosphoprotein Swa2p is required for clathrin function in yeast."
    Gall W.E., Higginbotham M.A., Chen C.-Y., Ingram M.F., Cyr D.M., Graham T.R.
    Curr. Biol. 10:1349-1358(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SWA2.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-49 AND SER-52, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-52, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCLC1_YEAST
AccessioniPrimary (citable) accession number: P17891
Secondary accession number(s): D6VUV0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: June 24, 2015
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

CLC1 binds calcium, and calmodulin in presence of calcium.
Present with 3490 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.