Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P17891 (CLC1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Clathrin light chain

Short name=CLC
Gene names
Name:CLC1
Ordered Locus Names:YGR167W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length233 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. In yeast, it is involved in the retention of proteins in an intracellular membrane compartment, presumably the trans-Golgi. The yeast light chain is important for cell growth. The light chain may help to properly orient the assembly/ disassembly of the clathrin coats.

Subunit structure

Clathrin coats are formed from molecules containing 3 heavy chains and 3 light chains. Interacts with the auxilin-like clathrin uncoating factor SWA2. Ref.6

Subcellular location

Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Membranecoated pit; Peripheral membrane protein; Cytoplasmic side. Note: Cytoplasmic face of coated pits and vesicles.

Miscellaneous

CLC1 binds calcium, and calmodulin in presence of calcium.

Present with 3490 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the clathrin light chain family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 233233Clathrin light chain
PRO_0000205776

Regions

Region144 – 20461Involved in binding clathrin heavy chain Potential
Coiled coil125 – 18662 Potential

Amino acid modifications

Modified residue491Phosphothreonine Ref.8
Modified residue521Phosphoserine Ref.8 Ref.9

Sequences

Sequence LengthMass (Da)Tools
P17891 [UniParc].

Last modified November 1, 1990. Version 1.
Checksum: 27BAB175780EC8B3

FASTA23326,532
        10         20         30         40         50         60 
MSEKFPPLED QNIDFTPNDK KDDDTDFLKR EAEILGDEFK TEQDDILETE ASPAKDDDEI 

        70         80         90        100        110        120 
RDFEEQFPDI NSANGAVSSD QNGSATVSSG NDNGEADDDF STFEGANQST ESVKEDRSEV 

       130        140        150        160        170        180 
VDQWKQRRAV EIHEKDLKDE ELKKELQDEA IKHIDDFYDS YNKKKEQQLE DAAKEAEAFL 

       190        200        210        220        230 
KKRDEFFGQD NTTWDRALQL INQDDADIIG GRDRSKLKEI LLRLKGNAKA PGA 

« Hide

References

« Hide 'large scale' references
[1]"Yeast clathrin has a distinctive light chain that is important for cell growth."
Silveira L.A., Wong D.H., Masiarz F.R., Schekman R.
J. Cell Biol. 111:1437-1449(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 31-39.
[2]"Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"The auxilin-like phosphoprotein Swa2p is required for clathrin function in yeast."
Gall W.E., Higginbotham M.A., Chen C.-Y., Ingram M.F., Cyr D.M., Graham T.R.
Curr. Biol. 10:1349-1358(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SWA2.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-49 AND SER-52, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-52, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X52272 Genomic DNA. Translation: CAA36515.1.
Z72952 Genomic DNA. Translation: CAA97192.1.
Z72953 Genomic DNA. Translation: CAA97193.1.
AY558272 Genomic DNA. Translation: AAS56598.1.
BK006941 Genomic DNA. Translation: DAA08261.1.
PIRA36425.
RefSeqNP_011683.3. NM_001181296.3.

3D structure databases

ProteinModelPortalP17891.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33419. 399 interactions.
DIPDIP-2280N.
IntActP17891. 12 interactions.
MINTMINT-527297.
STRING4932.YGR167W.

Proteomic databases

PaxDbP17891.
PeptideAtlasP17891.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR167W; YGR167W; YGR167W.
GeneID853077.
KEGGsce:YGR167W.

Organism-specific databases

CYGDYGR167w.
SGDS000003399. CLC1.

Phylogenomic databases

eggNOGNOG251359.
HOGENOMHOG000190326.
OMASTFESQF.
OrthoDBEOG7Z95XB.

Enzyme and pathway databases

BioCycYEAST:G3O-30865-MONOMER.

Gene expression databases

GenevestigatorP17891.

Family and domain databases

InterProIPR000996. Clathrin_L-chain.
[Graphical view]
PANTHERPTHR10639. PTHR10639. 1 hit.
PfamPF01086. Clathrin_lg_ch. 1 hit.
[Graphical view]
PROSITEPS00581. CLATHRIN_LIGHT_CHN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio973038.

Entry information

Entry nameCLC1_YEAST
AccessionPrimary (citable) accession number: P17891
Secondary accession number(s): D6VUV0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: April 16, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families