P17891 (CLC1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Clathrin light chain
Short name=CLC

Gene names

Name:	CLC1
Ordered Locus Names:	YGR167W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	233 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. In yeast, it is involved in the retention of proteins in an intracellular membrane compartment, presumably the trans-Golgi. The yeast light chain is important for cell growth. The light chain may help to properly orient the assembly/ disassembly of the clathrin coats.
Subunit structure	Clathrin coats are formed from molecules containing 3 heavy chains and 3 light chains. Interacts with the auxilin-like clathrin uncoating factor SWA2. Ref.6
Subcellular location	Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Membrane › coated pit; Peripheral membrane protein; Cytoplasmic side. Note: Cytoplasmic face of coated pits and vesicles.
Miscellaneous	CLC1 binds calcium, and calmodulin in presence of calcium. Present with 3490 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the clathrin light chain family.

Ontologies

Keywords
Cellular component	Coated pit Cytoplasmic vesicle Membrane
Domain	Coiled coil
Ligand	Calcium Calmodulin-binding
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	endocytosis Inferred from direct assay PubMed 16643280. Source: SGD intracellular protein transport Inferred from electronic annotation. Source: InterPro
Cellular_component	clathrin coat of coated pit Inferred from electronic annotation. Source: InterPro clathrin coat of trans-Golgi network vesicle Inferred from electronic annotation. Source: InterPro clathrin vesicle coat Traceable author statement PubMed 9171338. Source: SGD
Molecular_function	structural molecule activity Traceable author statement PubMed 9171338. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 233	233	Clathrin light chain		PRO_0000205776
Regions
	Region	144 – 204	61	Involved in binding clathrin heavy chain Potential
	Coiled coil	125 – 186	62	Potential
Amino acid modifications
	Modified residue	49	1	Phosphothreonine Ref.8
	Modified residue	52	1	Phosphoserine Ref.8 Ref.9

Sequences

	Sequence		Length	Mass (Da)
	P17891 [UniParc]. Last modified November 1, 1990. Version 1. Checksum: 27BAB175780EC8B3	FASTA	233	26,532
	10 20 30 40 50 60 MSEKFPPLED QNIDFTPNDK KDDDTDFLKR EAEILGDEFK TEQDDILETE ASPAKDDDEI 70 80 90 100 110 120 RDFEEQFPDI NSANGAVSSD QNGSATVSSG NDNGEADDDF STFEGANQST ESVKEDRSEV 130 140 150 160 170 180 VDQWKQRRAV EIHEKDLKDE ELKKELQDEA IKHIDDFYDS YNKKKEQQLE DAAKEAEAFL 190 200 210 220 230 KKRDEFFGQD NTTWDRALQL INQDDADIIG GRDRSKLKEI LLRLKGNAKA PGA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Yeast clathrin has a distinctive light chain that is important for cell growth." Silveira L.A., Wong D.H., Masiarz F.R., Schekman R. J. Cell Biol. 111:1437-1449(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 31-39.
[2]	"Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII." Rieger M., Brueckner M., Schaefer M., Mueller-Auer S. Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"The auxilin-like phosphoprotein Swa2p is required for clathrin function in yeast." Gall W.E., Higginbotham M.A., Chen C.-Y., Ingram M.F., Cyr D.M., Graham T.R. Curr. Biol. 10:1349-1358(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SWA2.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-49 AND SER-52, MASS SPECTROMETRY. Strain: ADR376.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-52, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X52272 Genomic DNA. Translation: CAA36515.1. Z72952 Genomic DNA. Translation: CAA97192.1. Z72953 Genomic DNA. Translation: CAA97193.1. AY558272 Genomic DNA. Translation: AAS56598.1. BK006941 Genomic DNA. Translation: DAA08261.1.
PIR	A36425.
RefSeq	NP_011683.3. NM_001181296.3.
3D structure databases
ProteinModelPortal	P17891.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2280N.
IntAct	P17891. 12 interactions.
MINT	MINT-527297.
STRING	4932.YGR167W.
Proteomic databases
PaxDb	P17891.
PeptideAtlas	P17891.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR167W; YGR167W; YGR167W.
GeneID	853077.
KEGG	sce:YGR167W. sce:YGR172C.
Organism-specific databases
CYGD	YGR167w.
SGD	S000003399. CLC1.
Phylogenomic databases
eggNOG	NOG251359.
HOGENOM	HOG000190326.
OMA	GTSWERI.
OrthoDB	EOG4HX895.
Gene expression databases
Genevestigator	P17891.
GermOnline	YGR167W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000996. Clathrin_L-chain. [Graphical view]
PANTHER	PTHR10639. PTHR10639. 1 hit.
Pfam	PF01086. Clathrin_lg_ch. 1 hit. [Graphical view]
PROSITE	PS00224. CLATHRIN_LIGHT_CHN_1. False negative. PS00581. CLATHRIN_LIGHT_CHN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	973038.

Entry information

Entry name

CLC1_YEAST

Accession

Primary (citable) accession number: P17891
Secondary accession number(s): D6VUV0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	April 3, 2013
This is version 119 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program