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Protein

Superkiller protein 3

Gene

SKI3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the SKI complex involved in 3'-mRNA degradation pathway. Represses dsRNA virus propagation by specifically blocking translation of viral mRNAs, perhaps recognizing the absence of CAP or poly(A). Essential for cell growth only in the presence of M1 replicon.9 Publications

Miscellaneous

Present with 11900 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

  • defense response to virus Source: UniProtKB-KW
  • nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay Source: SGD
  • nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5' Source: SGD
  • nuclear-transcribed mRNA catabolic process, non-stop decay Source: SGD

Keywordsi

Biological processAntiviral defense

Enzyme and pathway databases

BioCyciYEAST:G3O-34312-MONOMER
ReactomeiR-SCE-429958 mRNA decay by 3' to 5' exoribonuclease

Names & Taxonomyi

Protein namesi
Recommended name:
Superkiller protein 3
Gene namesi
Name:SKI3
Ordered Locus Names:YPR189W
ORF Names:P9677.7
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR189W
SGDiS000006393 SKI3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001063231 – 1432Superkiller protein 3Add BLAST1432

Proteomic databases

MaxQBiP17883
PaxDbiP17883
PRIDEiP17883

Interactioni

Subunit structurei

Component of the SKI complex composed of at least SKI2, SKI3 and SKI8. The SKI complex interacts with SKI7, which makes the link between the SKI complex and the exosome in order to perform mRNA degradation.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi36361, 212 interactors
DIPiDIP-6379N
IntActiP17883, 40 interactors
MINTiP17883
STRINGi4932.YPR189W

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4BUJX-ray3.70B/F1-1432[»]
5MC6electron microscopy3.80i1-1432[»]
ProteinModelPortaliP17883
SMRiP17883
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati4 – 37TPR 1Add BLAST34
Repeati47 – 80TPR 2Add BLAST34
Repeati425 – 458TPR 3Add BLAST34
Repeati471 – 507TPR 4Add BLAST37
Repeati508 – 541TPR 5Add BLAST34
Repeati627 – 661TPR 6Add BLAST35
Repeati702 – 735TPR 7Add BLAST34
Repeati736 – 769TPR 8Add BLAST34
Repeati945 – 985TPR 9Add BLAST41
Repeati987 – 1018TPR 10Add BLAST32
Repeati1226 – 1259TPR 11Add BLAST34

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

GeneTreeiENSGT00390000016407
HOGENOMiHOG000247860
InParanoidiP17883
KOiK12600
OMAiAWTNLGT
OrthoDBiEOG092C0F8J

Family and domain databases

Gene3Di1.25.40.10, 3 hits
InterProiView protein in InterPro
IPR013026 TPR-contain_dom
IPR011990 TPR-like_helical_dom_sf
IPR019734 TPR_repeat
PfamiView protein in Pfam
PF13181 TPR_8, 2 hits
SMARTiView protein in SMART
SM00028 TPR, 8 hits
SUPFAMiSSF48452 SSF48452, 4 hits
PROSITEiView protein in PROSITE
PS50005 TPR, 7 hits
PS50293 TPR_REGION, 5 hits

Sequencei

Sequence statusi: Complete.

P17883-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDIKQLLKE AKQELTNRDY EETIEISEKV LKLDPDNYFA HIFLGKALSS
60 70 80 90 100
LPASNNVSSN RNLERATNHY VSAAKLVPDN LLAWKGLFLL FRTTEVVPDI
110 120 130 140 150
LSYDEYFDLC GQYADALLKQ EQSQVELIND IKLLKKTHPD CQKAFYQHLK
160 170 180 190 200
PGSLMAETIG RHLSTPQDAL LNLIKILSNI ETTEIGKTLS QNRLKLKASD
210 220 230 240 250
PDYQIKLNSF SWEIIKNSEI DQLYNQLVNI LADDQKRSEI ENQWLEYRIK
260 270 280 290 300
VLKSMPLDVK KDFFTKVKEM VEDMVLVNHQ SLLAWQKYFE WTDYEDLDNM
310 320 330 340 350
DAPLIIKYFK KFPKDPLAMI LYSWLSSKLS KYDIKSLESA NKPPEGHKKT
360 370 380 390 400
EKETDIKDVD ETNEDEVKDR VEDEVKDRVE DEVKDQDEEA KEDEEEDLDD
410 420 430 440 450
IEIGLLEEEV VTVLTENIVK CKNNILAHRI LCQYYLLTKE YEAALPYIKN
460 470 480 490 500
GISLIAYNIK DLGVHLPLTK REFSLDLATV YTYVDAPKDH NAALKLYDNI
510 520 530 540 550
LSGDFSNIQA KMGKGIIFIE RKNWKDAMTL LTQVHEQSPN NLEVLSELSW
560 570 580 590 600
SKAHMGYMDE ALAGLDTVIK GIKGMDLRSI DFRALNLWRQ AKVYIMKHAS
610 620 630 640 650
INDAKQENVK CAFKLLIQSI KILDTFAPGF STLGDIYCHY YKDHLRAFKC
660 670 680 690 700
YFKAFDLDAG DYTAAKYITE TYASKPNWQA ASSIASRLIK GEKAKAELRS
710 720 730 740 750
NNWPFRVVGI AHLEKQEESD SIEWFQSALR VDPNDVESWV GLGQAYHACG
760 770 780 790 800
RIEASIKVFD KAIQLRPSHT FAQYFKAISL CDVGEYLESL DILEKVCQEA
810 820 830 840 850
ATEESFQIGL VEVLMRCSLD LYSQGFLLKS VSIAKDTIER IKIIISELKC
860 870 880 890 900
ENQQVWIYLS QVLRLFIWIE SKVDTLPVES LVSIFENSQF SGSEEIDSVD
910 920 930 940 950
NIKIDTLLDS TTDDNVSIAC KFLILASKYS VSDQKFTDIA GTVRASYWYN
960 970 980 990 1000
IGISELTAFI TLKEPQYRDA AIFAFKKSIQ LQSNTSETWI GLGIATMDIN
1010 1020 1030 1040 1050
FRVSQHCFIK ATALEPKATN TWFNLAMLGL KKKDTEFAQQ VLNKLQSLAP
1060 1070 1080 1090 1100
QDSSPWLGMA LILEEQGDII GSSKLFAHSF ILSNGRSKAA QFMYAKNVLE
1110 1120 1130 1140 1150
NHINNGDDER DIETVEKLTT ASIALEQFFK KSPDSQFALQ CALLTLERLH
1160 1170 1180 1190 1200
HYENANELAN RLIGILEKKF EKTQDERELF NFAIIKGQFA RIHLGLGNFE
1210 1220 1230 1240 1250
LSIENADLSQ GIISESSDEK SMKTKISNHI CLGLSYFFLN DFDQTLNQFQ
1260 1270 1280 1290 1300
ELLSISKDSK HLVVLIAKVL YDVGESDTKE IALQELTEYI ATSGADLLVT
1310 1320 1330 1340 1350
LTIAAMSILD DKREDLSIIL EELKALPLSK QIIDKHKDAP YLIEEITKRL
1360 1370 1380 1390 1400
YRNDTGKQVW QRSAYFFPNN LKVWERLDKN IQRRIASNGQ NKVTAEEMSK
1410 1420 1430
LYCESKNLRS IQRGMFLCPW NVTAVKALNE CF
Length:1,432
Mass (Da):163,726
Last modified:May 15, 2002 - v2
Checksum:i18FFF78D7CA5A6E4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti985T → I in AAA50573 (PubMed:2660461).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36940 Genomic DNA Translation: AAA50573.1
U25841 Genomic DNA Translation: AAB64618.1
BK006949 Genomic DNA Translation: DAA11605.1
PIRiS58819
RefSeqiNP_015515.1, NM_001184286.1

Genome annotation databases

EnsemblFungiiYPR189W; YPR189W; YPR189W
GeneIDi856319
KEGGisce:YPR189W

Similar proteinsi

Entry informationi

Entry nameiSKI3_YEAST
AccessioniPrimary (citable) accession number: P17883
Secondary accession number(s): Q06585
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: May 15, 2002
Last modified: May 23, 2018
This is version 177 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

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