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Protein

Heat shock 70 kDa protein 1B

Gene

Hspa1b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. Essential for STUB1-mediated ubiquitination and degradation of FOXP3 in regulatory T-cells (Treg) during inflammation.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei71 – 711ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 154ATPBy similarity
Nucleotide bindingi202 – 2043ATPBy similarity
Nucleotide bindingi268 – 2758ATPBy similarity
Nucleotide bindingi339 – 3424ATPBy similarity

GO - Molecular functioni

GO - Biological processi

  • binding of sperm to zona pellucida Source: MGI
  • negative regulation of apoptotic process Source: MGI
  • negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
  • response to heat Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Heat shock 70 kDa protein 1B
Alternative name(s):
Heat shock 70 kDa protein 1
Short name:
HSP70.1
Gene namesi
Name:Hspa1b
Synonyms:Hcp70.1, Hsp70-1, Hsp70a1, Hspa1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:99517. Hspa1b.

Subcellular locationi

  • Cytoplasm By similarity

  • Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs.By similarity

GO - Cellular componenti

  • cell body Source: MGI
  • intracellular Source: MGI
  • mitochondrial matrix Source: MGI
  • mitochondrion Source: MGI
  • zona pellucida receptor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 642641Heat shock 70 kDa protein 1BPRO_0000078251Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei108 – 1081N6-acetyllysineBy similarity
Modified residuei246 – 2461N6-acetyllysineBy similarity
Modified residuei348 – 3481N6-acetyllysineBy similarity
Modified residuei561 – 5611N6,N6,N6-trimethyllysine; by METTL21ABy similarity
Modified residuei632 – 6321PhosphoserineBy similarity
Modified residuei634 – 6341PhosphoserineBy similarity
Modified residuei637 – 6371PhosphothreonineBy similarity

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiP17879.
MaxQBiP17879.
PaxDbiP17879.
PeptideAtlasiP17879.
PRIDEiP17879.

PTM databases

PhosphoSiteiP17879.

Expressioni

Tissue specificityi

Testis-specific.

Gene expression databases

BgeeiP17879.
CleanExiMM_HSPA1B.
GenevisibleiP17879. MM.

Interactioni

Subunit structurei

Component of the CatSper complex (PubMed:17478420, PubMed:19516020). Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs (By similarity). Interacts with CHCHD3, DNAJC7, IRAK1BP1, PPP5C and TSC2 (By similarity). Interacts with TERT; the interaction occurs in the absence of the RNA component, TERC, and dissociates once the TERT complex has formed (By similarity). Interacts with TRIM5 (via B30.2/SPRY domain) (By similarity). Interacts with METTL21A (By similarity). Interacts with PARK2 (By similarity). Interacts with FOXP3 (PubMed:23973223). Interacts with NOD2; the interaction enhances NOD2 stability (By similarity). Interacts with DNAJC9 (via J domain) (By similarity). Interacts with ATF5; the interaction protects ATF5 from degradation via proteasome-dependent and caspase-dependent processes (By similarity).By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
LoxP283012EBI-397360,EBI-642911
Traf6P701963EBI-397360,EBI-448028

Protein-protein interaction databases

BioGridi200452. 36 interactions.
DIPiDIP-31549N.
IntActiP17879. 11 interactions.
MINTiMINT-2516359.
STRINGi10090.ENSMUSP00000128525.

Structurei

3D structure databases

ProteinModelPortaliP17879.
SMRiP17879. Positions 1-613.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the heat shock protein 70 family.Curated

Phylogenomic databases

eggNOGiKOG0101. Eukaryota.
COG0443. LUCA.
GeneTreeiENSGT00820000127001.
HOGENOMiHOG000228135.
HOVERGENiHBG051845.
InParanoidiP17879.
KOiK03283.
OMAiCSETIAW.
OrthoDBiEOG7PCJGF.
PhylomeDBiP17879.
TreeFamiTF105042.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
InterProiIPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
PROSITEiPS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17879-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKNTAIGID LGTTYSCVGV FQHGKVEIIA NDQGNRTTPS YVAFTDTERL
60 70 80 90 100
IGDAAKNQVA LNPQNTVFDA KRLIGRKFGD AVVQSDMKHW PFQVVNDGDK
110 120 130 140 150
PKVQVNYKGE SRSFFPEEIS SMVLTKMKEI AEAYLGHPVT NAVITVPAYF
160 170 180 190 200
NDSQRQATKD AGVIAGLNVL RIINEPTAAA IAYGLDRTGK GERNVLIFDL
210 220 230 240 250
GGGTFDVSIL TIDDGIFEVK ATAGDTHLGG EDFDNRLVSH FVEEFKRKHK
260 270 280 290 300
KDISQNKRAV RRLRTACERA KRTLSSSTQA SLEIDSLFEG IDFYTSITRA
310 320 330 340 350
RFEELCSDLF RGTLEPVEKA LRDAKMDKAQ IHDLVLVGGS TRIPKVQKLL
360 370 380 390 400
QDFFNGRDLN KSINPDEAVA YGAAVQAAIL MGDKSENVQD LLLLDVAPLS
410 420 430 440 450
LGLETAGGVM TALIKRNSTI PTKQTQTFTT YSDNQPGVLI QVYEGERAMT
460 470 480 490 500
RDNNLLGRFE LSGIPPAPRG VPQIEVTFDI DANGILNVTA TDKSTGKANK
510 520 530 540 550
ITITNDKGRL SKEEIERMVQ EAERYKAEDE VQRDRVAAKN ALESYAFNMK
560 570 580 590 600
SAVEDEGLKG KLSEADKKKV LDKCQEVISW LDSNTLADKE EFVHKREELE
610 620 630 640
RVCSPIISGL YQGAGAPGAG GFGAQAPPKG ASGSGPTIEE VD
Length:642
Mass (Da):70,176
Last modified:October 24, 2003 - v3
Checksum:i4BB9B0B130C23D8B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35021 Genomic DNA. Translation: AAA37864.1.
AF109906 Genomic DNA. Translation: AAC84168.1.
CCDSiCCDS37592.1.
PIRiJH0095.
RefSeqiNP_034608.2. NM_010478.2.
UniGeneiMm.372314.

Genome annotation databases

EnsembliENSMUST00000172753; ENSMUSP00000133815; ENSMUSG00000090877.
GeneIDi15511.
KEGGimmu:15511.
UCSCiuc008ceo.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35021 Genomic DNA. Translation: AAA37864.1.
AF109906 Genomic DNA. Translation: AAC84168.1.
CCDSiCCDS37592.1.
PIRiJH0095.
RefSeqiNP_034608.2. NM_010478.2.
UniGeneiMm.372314.

3D structure databases

ProteinModelPortaliP17879.
SMRiP17879. Positions 1-613.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200452. 36 interactions.
DIPiDIP-31549N.
IntActiP17879. 11 interactions.
MINTiMINT-2516359.
STRINGi10090.ENSMUSP00000128525.

PTM databases

PhosphoSiteiP17879.

Proteomic databases

EPDiP17879.
MaxQBiP17879.
PaxDbiP17879.
PeptideAtlasiP17879.
PRIDEiP17879.

Protocols and materials databases

DNASUi15511.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000172753; ENSMUSP00000133815; ENSMUSG00000090877.
GeneIDi15511.
KEGGimmu:15511.
UCSCiuc008ceo.1. mouse.

Organism-specific databases

CTDi3304.
MGIiMGI:99517. Hspa1b.

Phylogenomic databases

eggNOGiKOG0101. Eukaryota.
COG0443. LUCA.
GeneTreeiENSGT00820000127001.
HOGENOMiHOG000228135.
HOVERGENiHBG051845.
InParanoidiP17879.
KOiK03283.
OMAiCSETIAW.
OrthoDBiEOG7PCJGF.
PhylomeDBiP17879.
TreeFamiTF105042.

Miscellaneous databases

PROiP17879.
SOURCEiSearch...

Gene expression databases

BgeeiP17879.
CleanExiMM_HSPA1B.
GenevisibleiP17879. MM.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
InterProiIPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
PROSITEiPS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization and sequence of a mouse hsp70 gene and its expression in mouse cell lines."
    Hunt C., Calderwood S.
    Gene 87:199-204(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: A/J.
  2. "Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse."
    Xie T., Rowen L., Aguado B., Ahearn M.E., Madan A., Qin S., Campbell R.D., Hood L.
    Genome Res. 13:2621-2636(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 129.
  3. "CatSperbeta, a novel transmembrane protein in the CatSper channel complex."
    Liu J., Xia J., Cho K.-H., Clapham D.E., Ren D.
    J. Biol. Chem. 282:18945-18952(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN A COMPLEX WITH CATSPER1 AND CATSPERB.
  4. "A novel, single, transmembrane protein CATSPERG is associated with CATSPER1 channel protein."
    Wang H., Liu J., Cho K.-H., Ren D.
    Biol. Reprod. 81:539-544(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN A COMPLEX WITH CATSPER1; CATSPERB AND CATSPERG2.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney.
  6. "The ubiquitin ligase Stub1 negatively modulates regulatory T cell suppressive activity by promoting degradation of the transcription factor Foxp3."
    Chen Z., Barbi J., Bu S., Yang H.Y., Li Z., Gao Y., Jinasena D., Fu J., Lin F., Chen C., Zhang J., Yu N., Li X., Shan Z., Nie J., Gao Z., Tian H., Li Y.
    , Yao Z., Zheng Y., Park B.V., Pan Z., Zhang J., Dang E., Li Z., Wang H., Luo W., Li L., Semenza G.L., Zheng S.G., Loser K., Tsun A., Greene M.I., Pardoll D.M., Pan F., Li B.
    Immunity 39:272-285(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH FOXP3.

Entry informationi

Entry nameiHS71B_MOUSE
AccessioniPrimary (citable) accession number: P17879
Secondary accession number(s): Q61689, Q925V6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: October 24, 2003
Last modified: July 6, 2016
This is version 147 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.