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Protein

Heat shock 70 kDa protein 1B

Gene

Hspa1b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The co-chaperones have been shown to not only regulate different steps of the ATPase cycle, but they also have an individual specificity such that one co-chaperone may promote folding of a substrate while another may promote degradation. The affinity for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release. The co-chaperones are of three types: J-domain co-chaperones such as HSP40s (stimulate ATPase hydrolysis by HSP70), the nucleotide exchange factors (NEF) such as BAG1/2/3 (facilitate conversion of HSP70 from the ADP-bound to the ATP-bound state thereby promoting substrate release), and the TPR domain chaperones such as HOPX and STUB1. Maintains protein homeostasis during cellular stress through two opposing mechanisms: protein refolding and degradation. Its acetylation/deacetylation state determines whether it functions in protein refolding or protein degradation by controlling the competitive binding of co-chaperones HOPX and STUB1. During the early stress response, the acetylated form binds to HOPX which assists in chaperone-mediated protein refolding, thereafter, it is deacetylated and binds to ubiquitin ligase STUB1 that promotes ubiquitin-mediated protein degradation. Regulates centrosome integrity during mitosis, and is required for the maintenance of a functional mitotic centrosome that supports the assembly of a bipolar mitotic spindle. Enhances STUB1-mediated SMAD3 ubiquitination and degradation and facilitates STUB1-mediated inhibition of TGF-beta signaling. Essential for STUB1-mediated ubiquitination and degradation of FOXP3 in regulatory T-cells (Treg) during inflammation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei71ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 15ATPBy similarity4
Nucleotide bindingi202 – 204ATPBy similarity3
Nucleotide bindingi268 – 275ATPBy similarity8
Nucleotide bindingi339 – 342ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

  • binding of sperm to zona pellucida Source: MGI
  • negative regulation of apoptotic process Source: MGI
  • negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
  • positive regulation of microtubule nucleation Source: UniProtKB
  • protein refolding Source: UniProtKB
  • regulation of mitotic spindle assembly Source: UniProtKB
  • response to heat Source: MGI

Keywordsi

Molecular functionChaperone
Biological processStress response
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-3371453. Regulation of HSF1-mediated heat shock response.
R-MMU-3371497. HSP90 chaperone cycle for steroid hormone receptors (SHR).
R-MMU-3371568. Attenuation phase.
R-MMU-3371571. HSF1-dependent transactivation.
R-MMU-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-MMU-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Heat shock 70 kDa protein 1B
Alternative name(s):
Heat shock 70 kDa protein 1
Short name:
HSP70.1
Gene namesi
Name:Hspa1b
Synonyms:Hcp70.1, Hsp70-1, Hsp70a1, Hspa1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:99517. Hspa1b.

Subcellular locationi

  • Cytoplasm By similarity
  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity

  • Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs.By similarity

GO - Cellular componenti

  • cell body Source: MGI
  • centrosome Source: UniProtKB
  • intracellular Source: MGI
  • mitochondrial matrix Source: MGI
  • mitochondrion Source: MGI
  • zona pellucida receptor complex Source: MGI

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000782512 – 642Heat shock 70 kDa protein 1BAdd BLAST641

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei77N6-acetyllysineBy similarity1
Modified residuei108N6-acetyllysineBy similarity1
Modified residuei246N6-acetyllysineBy similarity1
Modified residuei348N6-acetyllysineBy similarity1
Modified residuei469Omega-N-methylarginineBy similarity1
Modified residuei561N6,N6,N6-trimethyllysine; by METTL21A; alternateBy similarity1
Modified residuei561N6,N6-dimethyllysine; alternateBy similarity1
Modified residuei632PhosphoserineBy similarity1
Modified residuei634PhosphoserineBy similarity1
Modified residuei637PhosphothreonineBy similarity1

Post-translational modificationi

In response to cellular stress, acetylated at Lys-77 by NA110 and then gradually deacetylated by HDAC4 at later stages. Acetylation enhances its chaperone activity and also determines whether it will function as a chaperone for protein refolding or degradation by controlling its binding to co-chaperones HOPX and STUB1. The acetylated form and the non-acetylated form bind to HOPX and STUB1 respectively. Acetylation also protects cells against various types of cellular stress.By similarity

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

MaxQBiP17879.
PaxDbiP17879.
PeptideAtlasiP17879.
PRIDEiP17879.

Expressioni

Tissue specificityi

Testis-specific.

Gene expression databases

BgeeiENSMUSG00000090877.
CleanExiMM_HSPA1B.
GenevisibleiP17879. MM.

Interactioni

Subunit structurei

Component of the CatSper complex (PubMed:17478420, PubMed:19516020). Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs (By similarity). Interacts with CHCHD3, DNAJC7, IRAK1BP1, PPP5C and TSC2 (By similarity). Interacts with TERT; the interaction occurs in the absence of the RNA component, TERC, and dissociates once the TERT complex has formed (By similarity). Interacts with TRIM5 (via B30.2/SPRY domain) (By similarity). Interacts with METTL21A (By similarity). Interacts with PRKN (By similarity). Interacts with FOXP3 (PubMed:23973223). Interacts with NOD2; the interaction enhances NOD2 stability (By similarity). Interacts with DNAJC9 (via J domain) (By similarity). Interacts with ATF5; the interaction protects ATF5 from degradation via proteasome-dependent and caspase-dependent processes. Interacts with NAA10, HSP40, HSP90 and HDAC4. The acetylated form and the non-acetylated form interact with HOPX and STUB1 respectively. Interacts with NEDD1 and SMAD3. Interacts (via NBD) with BAG1, BAG2, BAG3 and HSPH1/HSP105 (By similarity).By similarity3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi200452. 39 interactors.
DIPiDIP-31549N.
IntActiP17879. 13 interactors.
MINTiMINT-2516359.
STRINGi10090.ENSMUSP00000128525.

Structurei

3D structure databases

ProteinModelPortaliP17879.
SMRiP17879.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 386Nucleotide-binding domain (NBD)By similarityAdd BLAST385
Regioni394 – 509Substrate-binding domain (SBD)By similarityAdd BLAST116

Domaini

The N-terminal nucleotide binding domain (NBD) (also known as the ATPase domain) is responsible for binding and hydrolyzing ATP. The C-terminal substrate-binding domain (SBD) (also known as peptide-binding domain) binds to the client/substrate proteins. The two domains are allosterically coupled so that, when ATP is bound to the NBD, the SBD binds relatively weakly to clients. When ADP is bound in the NBD, a conformational change enhances the affinity of the SBD for client proteins.By similarity

Sequence similaritiesi

Belongs to the heat shock protein 70 family.Curated

Phylogenomic databases

eggNOGiKOG0101. Eukaryota.
COG0443. LUCA.
GeneTreeiENSGT00890000139776.
HOGENOMiHOG000228135.
HOVERGENiHBG051845.
InParanoidiP17879.
KOiK03283.
OMAiVDWLDHN.
OrthoDBiEOG091G03SF.
PhylomeDBiP17879.
TreeFamiTF105042.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
InterProiView protein in InterPro
IPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
PfamiView protein in Pfam
PF00012. HSP70. 1 hit.
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
PROSITEiView protein in PROSITE
PS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17879-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKNTAIGID LGTTYSCVGV FQHGKVEIIA NDQGNRTTPS YVAFTDTERL
60 70 80 90 100
IGDAAKNQVA LNPQNTVFDA KRLIGRKFGD AVVQSDMKHW PFQVVNDGDK
110 120 130 140 150
PKVQVNYKGE SRSFFPEEIS SMVLTKMKEI AEAYLGHPVT NAVITVPAYF
160 170 180 190 200
NDSQRQATKD AGVIAGLNVL RIINEPTAAA IAYGLDRTGK GERNVLIFDL
210 220 230 240 250
GGGTFDVSIL TIDDGIFEVK ATAGDTHLGG EDFDNRLVSH FVEEFKRKHK
260 270 280 290 300
KDISQNKRAV RRLRTACERA KRTLSSSTQA SLEIDSLFEG IDFYTSITRA
310 320 330 340 350
RFEELCSDLF RGTLEPVEKA LRDAKMDKAQ IHDLVLVGGS TRIPKVQKLL
360 370 380 390 400
QDFFNGRDLN KSINPDEAVA YGAAVQAAIL MGDKSENVQD LLLLDVAPLS
410 420 430 440 450
LGLETAGGVM TALIKRNSTI PTKQTQTFTT YSDNQPGVLI QVYEGERAMT
460 470 480 490 500
RDNNLLGRFE LSGIPPAPRG VPQIEVTFDI DANGILNVTA TDKSTGKANK
510 520 530 540 550
ITITNDKGRL SKEEIERMVQ EAERYKAEDE VQRDRVAAKN ALESYAFNMK
560 570 580 590 600
SAVEDEGLKG KLSEADKKKV LDKCQEVISW LDSNTLADKE EFVHKREELE
610 620 630 640
RVCSPIISGL YQGAGAPGAG GFGAQAPPKG ASGSGPTIEE VD
Length:642
Mass (Da):70,176
Last modified:October 24, 2003 - v3
Checksum:i4BB9B0B130C23D8B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35021 Genomic DNA. Translation: AAA37864.1.
AF109906 Genomic DNA. Translation: AAC84168.1.
CCDSiCCDS37592.1.
PIRiJH0095.
RefSeqiNP_034608.2. NM_010478.2.
UniGeneiMm.372314.

Genome annotation databases

EnsembliENSMUST00000172753; ENSMUSP00000133815; ENSMUSG00000090877.
GeneIDi15511.
KEGGimmu:15511.
UCSCiuc008ceo.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiHS71B_MOUSE
AccessioniPrimary (citable) accession number: P17879
Secondary accession number(s): Q61689, Q925V6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: October 24, 2003
Last modified: August 30, 2017
This is version 158 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families