P17877 (GUN1_CLOJO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Endoglucanase 1 EC=3.2.1.4 Alternative name(s): Cellulase 1 Endo-1,4-beta-glucanase 1
Organism	Clostridium josui
Taxonomic identifier	1499 [NCBI]
Taxonomic lineage	cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	25 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This enzyme hydrolyzes cellotetraose, cellopentaose, and cellohexaose to cellobiose and cellotriose but does not hydrolyze cellobiose or cellotriose.
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Biophysicochemical properties	pH dependence: Optimum pH is 6.8 with carboxymethyl cellulose (CmC) as substrate. Temperature dependence: Optimum temperature is 60 degrees Celsius with carboxymethyl cellulose (CmC) as substrate.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›25	›25	Endoglucanase 1		PRO_0000184077
Experimental info
	Non-terminal residue	25	1

Sequences

	Sequence		Length	Mass (Da)
	P17877 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: F68D1B4D3BD9DFCB	FASTA	25	2,808
	10 20 YDASLKPNLQ IPQKNIPNND AVNIK

References

[1]	"Purification and properties of an endo-1,4-beta-glucanase from Clostridium josui." Fujino T., Sukhumavasi J., Sasaki T., Ohmiya K., Shimizu S. J. Bacteriol. 171:4076-4079(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE.

Cross-references

Sequence databases
PIR	A45920.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

GUN1_CLOJO

Accession

Primary (citable) accession number: P17877

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	April 3, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program