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Protein

Pectinesterase

Gene

pme1

Organism
Aspergillus tubingensis
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in maceration and soft-rotting of plant tissue.

Catalytic activityi

Pectin + n H2O = n methanol + pectate.

Pathwayi: pectin degradation

This protein is involved in step 1 of the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Pectinesterase (pme1)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway pectin degradation, which is itself part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin, the pathway pectin degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei138 – 1381SubstrateBy similarity
Sitei160 – 1601Transition state stabilizerBy similarity
Active sitei161 – 1611Proton donorPROSITE-ProRule annotation
Active sitei182 – 1821NucleophilePROSITE-ProRule annotation
Binding sitei247 – 2471SubstrateBy similarity
Binding sitei249 – 2491SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aspartyl esterase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

UniPathwayiUPA00545; UER00823.

Names & Taxonomyi

Protein namesi
Recommended name:
Pectinesterase (EC:3.1.1.11)
Short name:
PE
Alternative name(s):
Pectin methylesterase
Gene namesi
Name:pme1
OrganismiAspergillus tubingensis
Taxonomic identifieri5068 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717Add
BLAST
Chaini18 – 331314PectinesterasePRO_0000023473Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP17872.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pectinesterase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
IPR033131. Pectinesterase_Asp_AS.
IPR000070. Pectinesterase_cat.
IPR018040. Pectinesterase_Tyr_AS.
[Graphical view]
PfamiPF01095. Pectinesterase. 1 hit.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00800. PECTINESTERASE_1. 1 hit.
PS00503. PECTINESTERASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17872-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKSILASVL FAATALAASR MTAPSGAIVV AKSGGDYDTI SAAVDALSTT
60 70 80 90 100
STETQTIFIE EGSYDEQVYI PALSGKLIVY GQTEDTTTYT SNLVNITHAI
110 120 130 140 150
ALADVDNDDE TATLRNYAEG SAIYNLNIAN TCGQACHQAL AVSAYASEQG
160 170 180 190 200
YYACQFTGYQ DTLLAETGYQ VYAGTYIEGA VDFIFGQHAR AWFHECDIRV
210 220 230 240 250
LEGPSSASIT ANGRSSESDD SYYVIHKSTV AAADGNDVSS GTYYLGRPWS
260 270 280 290 300
QYARVCFQKT SMTDVINHLG WTEWSTSTPN TENVTFVEYG NTGTGAEGPR
310 320 330
ANFSSELTEP ITISWLLGSD WEDWVDTSYI N
Length:331
Mass (Da):35,715
Last modified:August 1, 1990 - v1
Checksum:i25B52150884C797A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52902 mRNA. Translation: CAA37084.1.
X54145 Genomic DNA. Translation: CAA38084.1.
PIRiJT0589.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52902 mRNA. Translation: CAA37084.1.
X54145 Genomic DNA. Translation: CAA38084.1.
PIRiJT0589.

3D structure databases

ProteinModelPortaliP17872.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00545; UER00823.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
IPR033131. Pectinesterase_Asp_AS.
IPR000070. Pectinesterase_cat.
IPR018040. Pectinesterase_Tyr_AS.
[Graphical view]
PfamiPF01095. Pectinesterase. 1 hit.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00800. PECTINESTERASE_1. 1 hit.
PS00503. PECTINESTERASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPME_ASPTU
AccessioniPrimary (citable) accession number: P17872
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: March 16, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Caution

Strain RH 5344 was previously said to be from A.niger.Curated

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.