P17856 (PROB_SERMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate 5-kinase
EC=2.7.2.11

Alternative name(s):
Gamma-glutamyl kinase
Short name=GK

Gene names

Name:

proB

Organism

Serratia marcescens

Taxonomic identifier

615 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Serratia

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline By similarity. HAMAP-Rule MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP-Rule MF_00456
Enzyme regulation	Proline-mediated feedback inhibition. HAMAP-Rule MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP-Rule MF_00456
Subcellular location	Cytoplasm HAMAP-Rule MF_00456.
Sequence similarities	Belongs to the glutamate 5-kinase family. Contains 1 PUA domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological_process	L-proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro glutamate 5-kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 367	367	Glutamate 5-kinase HAMAP-Rule MF_00456		PRO_0000109723
Regions
	Domain	275 – 353	79	PUA
	Nucleotide binding	169 – 170	2	ATP By similarity
	Nucleotide binding	211 – 217	7	ATP By similarity
Sites
	Binding site	10	1	ATP By similarity
	Binding site	50	1	Substrate By similarity
	Binding site	137	1	Substrate By similarity
	Binding site	149	1	Substrate; via amide nitrogen By similarity
Natural variations
	Natural variant	117	1	A → V in proline hyperproducing mutant; 700-X less sensitive to proline inhibition.

Sequences

	Sequence		Length	Mass (Da)
	P17856 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: 68A2AE8F78353AD5	FASTA	367	39,170
	10 20 30 40 50 60 MNGSQTLVVK LGTSVLTGGS LRLNRAHIVE LVRQCAQQHA AGHRIVIVTS GAIAAGREHL 70 80 90 100 110 120 GYPELPATIA SKQLLAAVGQ SRLIQLWEQL FSIYGIHVGQ MLLTRADLED RERFLNARDT 130 140 150 160 170 180 MTALLDNRIV PVINENDAVA TAEIKVGDND NLSALAAILA GADKLLLLTD QQGLYTADPR 190 200 210 220 230 240 NNPQAELIRE VHGIDDALRA IAGDSVSGLG TGGMGTKLQA ADVACRAGID VVIAAGSKPG 250 260 270 280 290 300 VVADVIEGKP VGTRFHALET PLENRKRWIF GAPPAGEITV DDGAVEAMMA RGSSLLPKGI 310 320 330 340 350 360 REVKGDFSRG EVIRIRNLTG RDLAHGVSRY NSDAMRMIAG HHSQEISEIL GYEYGPVAVH RDDMIVS

References

[1]	"Analysis of the Serratia marcescens proBA operon and feedback control of proline biosynthesis." Omori K., Suzuki S., Imai Y., Komatsubara S. J. Gen. Microbiol. 137:509-517(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sr41.
[2]	"Analysis of the mutant proBA operon from a proline-producing strain of Serratia marcescens." Omori K., Suzuki S., Imai Y., Komatsubara S. J. Gen. Microbiol. 138:693-699(1992) [PubMed] [Europe PMC] [Abstract] Cited for: MUTANT.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X53086 Genomic DNA. Translation: CAA37254.1. D90351 Genomic DNA. Translation: BAA14364.1.
PIR	KISEEM. A49753.
3D structure databases
ProteinModelPortal	P17856.
SMR	P17856. Positions 4-365.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00098; UER00359.
Family and domain databases
Gene3D	3.40.1160.10. 1 hit.
HAMAP	MF_00456. ProB.
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu/AcGlu_kinase. IPR011529. Glu_5kinase. IPR005715. Glu_5kinase/COase_Synthase. IPR019797. Glutamate_5-kinase_CS. IPR002478. PUA. IPR015947. PUA-like_domain. [Graphical view]
Pfam	PF00696. AA_kinase. 1 hit. PF01472. PUA. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SMART	SM00359. PUA. 1 hit. [Graphical view]
SUPFAM	SSF53633. Aa_kinase. 1 hit. SSF88697. PUA-like. 1 hit.
TIGRFAMs	TIGR01027. proB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB_SERMA

Accession

Primary (citable) accession number: P17856

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	April 3, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program