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P17856

- PROB_SERMA

UniProt

P17856 - PROB_SERMA

Protein

Glutamate 5-kinase

Gene

proB

Organism
Serratia marcescens
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (01 Aug 1990)
      Previous versions | rss
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    • Comment

    Functioni

    Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

    Catalytic activityi

    ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

    Enzyme regulationi

    Proline-mediated feedback inhibition.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei10 – 101ATPUniRule annotation
    Binding sitei50 – 501SubstrateUniRule annotation
    Binding sitei137 – 1371SubstrateUniRule annotation
    Binding sitei149 – 1491Substrate; via amide nitrogenUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi169 – 1702ATPUniRule annotation
    Nucleotide bindingi211 – 2177ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate 5-kinase activity Source: UniProtKB-HAMAP
    3. RNA binding Source: InterPro

    GO - Biological processi

    1. L-proline biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Proline biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00098; UER00359.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
    Alternative name(s):
    Gamma-glutamyl kinaseUniRule annotation
    Short name:
    GKUniRule annotation
    Gene namesi
    Name:proBUniRule annotation
    OrganismiSerratia marcescens
    Taxonomic identifieri615 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSerratia

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 367367Glutamate 5-kinasePRO_0000109723Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP17856.
    SMRiP17856. Positions 4-365.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini275 – 35379PUAUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glutamate 5-kinase family.UniRule annotation
    Contains 1 PUA domain.UniRule annotation

    Family and domain databases

    Gene3Di2.30.130.10. 1 hit.
    3.40.1160.10. 1 hit.
    HAMAPiMF_00456. ProB.
    InterProiIPR001048. Asp/Glu/Uridylate_kinase.
    IPR001057. Glu/AcGlu_kinase.
    IPR011529. Glu_5kinase.
    IPR005715. Glu_5kinase/COase_Synthase.
    IPR019797. Glutamate_5-kinase_CS.
    IPR002478. PUA.
    IPR015947. PUA-like_domain.
    [Graphical view]
    PfamiPF00696. AA_kinase. 1 hit.
    PF01472. PUA. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000729. GK. 1 hit.
    PRINTSiPR00474. GLU5KINASE.
    SMARTiSM00359. PUA. 1 hit.
    [Graphical view]
    SUPFAMiSSF53633. SSF53633. 1 hit.
    SSF88697. SSF88697. 1 hit.
    TIGRFAMsiTIGR01027. proB. 1 hit.
    PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
    PS50890. PUA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P17856-1 [UniParc]FASTAAdd to Basket

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    MNGSQTLVVK LGTSVLTGGS LRLNRAHIVE LVRQCAQQHA AGHRIVIVTS    50
    GAIAAGREHL GYPELPATIA SKQLLAAVGQ SRLIQLWEQL FSIYGIHVGQ 100
    MLLTRADLED RERFLNARDT MTALLDNRIV PVINENDAVA TAEIKVGDND 150
    NLSALAAILA GADKLLLLTD QQGLYTADPR NNPQAELIRE VHGIDDALRA 200
    IAGDSVSGLG TGGMGTKLQA ADVACRAGID VVIAAGSKPG VVADVIEGKP 250
    VGTRFHALET PLENRKRWIF GAPPAGEITV DDGAVEAMMA RGSSLLPKGI 300
    REVKGDFSRG EVIRIRNLTG RDLAHGVSRY NSDAMRMIAG HHSQEISEIL 350
    GYEYGPVAVH RDDMIVS 367
    Length:367
    Mass (Da):39,170
    Last modified:August 1, 1990 - v1
    Checksum:i68A2AE8F78353AD5
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti117 – 1171A → V in proline hyperproducing mutant; 700-X less sensitive to proline inhibition.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53086 Genomic DNA. Translation: CAA37254.1.
    D90351 Genomic DNA. Translation: BAA14364.1.
    PIRiA49753. KISEEM.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53086 Genomic DNA. Translation: CAA37254.1 .
    D90351 Genomic DNA. Translation: BAA14364.1 .
    PIRi A49753. KISEEM.

    3D structure databases

    ProteinModelPortali P17856.
    SMRi P17856. Positions 4-365.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00098 ; UER00359 .

    Family and domain databases

    Gene3Di 2.30.130.10. 1 hit.
    3.40.1160.10. 1 hit.
    HAMAPi MF_00456. ProB.
    InterProi IPR001048. Asp/Glu/Uridylate_kinase.
    IPR001057. Glu/AcGlu_kinase.
    IPR011529. Glu_5kinase.
    IPR005715. Glu_5kinase/COase_Synthase.
    IPR019797. Glutamate_5-kinase_CS.
    IPR002478. PUA.
    IPR015947. PUA-like_domain.
    [Graphical view ]
    Pfami PF00696. AA_kinase. 1 hit.
    PF01472. PUA. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000729. GK. 1 hit.
    PRINTSi PR00474. GLU5KINASE.
    SMARTi SM00359. PUA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53633. SSF53633. 1 hit.
    SSF88697. SSF88697. 1 hit.
    TIGRFAMsi TIGR01027. proB. 1 hit.
    PROSITEi PS00902. GLUTAMATE_5_KINASE. 1 hit.
    PS50890. PUA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of the Serratia marcescens proBA operon and feedback control of proline biosynthesis."
      Omori K., Suzuki S., Imai Y., Komatsubara S.
      J. Gen. Microbiol. 137:509-517(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Sr41.
    2. "Analysis of the mutant proBA operon from a proline-producing strain of Serratia marcescens."
      Omori K., Suzuki S., Imai Y., Komatsubara S.
      J. Gen. Microbiol. 138:693-699(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTANT.

    Entry informationi

    Entry nameiPROB_SERMA
    AccessioniPrimary (citable) accession number: P17856
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: August 1, 1990
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3