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P17856

- PROB_SERMA

UniProt

P17856 - PROB_SERMA

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Protein
Glutamate 5-kinase
Gene
proB
Organism
Serratia marcescens
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate By similarity.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Enzyme regulationi

Proline-mediated feedback inhibition.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei10 – 101ATP By similarity
Binding sitei50 – 501Substrate By similarity
Binding sitei137 – 1371Substrate By similarity
Binding sitei149 – 1491Substrate; via amide nitrogen By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi169 – 1702ATP By similarity
Nucleotide bindingi211 – 2177ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA binding Source: InterPro
  3. glutamate 5-kinase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. L-proline biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Proline biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinase (EC:2.7.2.11)
Alternative name(s):
Gamma-glutamyl kinase
Short name:
GK
Gene namesi
Name:proB
OrganismiSerratia marcescens
Taxonomic identifieri615 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSerratia

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 367367Glutamate 5-kinaseUniRule annotation
PRO_0000109723Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP17856.
SMRiP17856. Positions 4-365.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini275 – 35379PUA
Add
BLAST

Sequence similaritiesi

Contains 1 PUA domain.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
[Graphical view]
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SMARTiSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P17856-1 [UniParc]FASTAAdd to Basket

« Hide

MNGSQTLVVK LGTSVLTGGS LRLNRAHIVE LVRQCAQQHA AGHRIVIVTS    50
GAIAAGREHL GYPELPATIA SKQLLAAVGQ SRLIQLWEQL FSIYGIHVGQ 100
MLLTRADLED RERFLNARDT MTALLDNRIV PVINENDAVA TAEIKVGDND 150
NLSALAAILA GADKLLLLTD QQGLYTADPR NNPQAELIRE VHGIDDALRA 200
IAGDSVSGLG TGGMGTKLQA ADVACRAGID VVIAAGSKPG VVADVIEGKP 250
VGTRFHALET PLENRKRWIF GAPPAGEITV DDGAVEAMMA RGSSLLPKGI 300
REVKGDFSRG EVIRIRNLTG RDLAHGVSRY NSDAMRMIAG HHSQEISEIL 350
GYEYGPVAVH RDDMIVS 367
Length:367
Mass (Da):39,170
Last modified:August 1, 1990 - v1
Checksum:i68A2AE8F78353AD5
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti117 – 1171A → V in proline hyperproducing mutant; 700-X less sensitive to proline inhibition.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X53086 Genomic DNA. Translation: CAA37254.1.
D90351 Genomic DNA. Translation: BAA14364.1.
PIRiA49753. KISEEM.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X53086 Genomic DNA. Translation: CAA37254.1 .
D90351 Genomic DNA. Translation: BAA14364.1 .
PIRi A49753. KISEEM.

3D structure databases

ProteinModelPortali P17856.
SMRi P17856. Positions 4-365.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00098 ; UER00359 .

Family and domain databases

Gene3Di 2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPi MF_00456. ProB.
InterProi IPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
[Graphical view ]
Pfami PF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
[Graphical view ]
PIRSFi PIRSF000729. GK. 1 hit.
PRINTSi PR00474. GLU5KINASE.
SMARTi SM00359. PUA. 1 hit.
[Graphical view ]
SUPFAMi SSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsi TIGR01027. proB. 1 hit.
PROSITEi PS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Analysis of the Serratia marcescens proBA operon and feedback control of proline biosynthesis."
    Omori K., Suzuki S., Imai Y., Komatsubara S.
    J. Gen. Microbiol. 137:509-517(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Sr41.
  2. "Analysis of the mutant proBA operon from a proline-producing strain of Serratia marcescens."
    Omori K., Suzuki S., Imai Y., Komatsubara S.
    J. Gen. Microbiol. 138:693-699(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTANT.

Entry informationi

Entry nameiPROB_SERMA
AccessioniPrimary (citable) accession number: P17856
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: September 3, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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