P17847 (NIR_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ferredoxin--nitrite reductase, chloroplastic
EC=1.7.7.1

Gene names

Name:

NIR

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACMAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	569 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	NH3 + 2 H2O + 6 oxidized ferredoxin = nitrite + 6 reduced ferredoxin + 7 H+.
Cofactor	Binds 1 siroheme per subunit. Binds 1 4Fe-4S cluster per subunit By similarity.
Pathway	Nitrogen metabolism; nitrate reduction (assimilation).
Subunit structure	Monomer.
Subcellular location	Plastid › chloroplast.
Induction	By nitrate.
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.
Sequence caution	The sequence AAA60450.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Electron transport Nitrate assimilation Transport
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	4Fe-4S Heme Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrate assimilation Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW ferredoxin-nitrite reductase activity Inferred from electronic annotation. Source: EC heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	‹1 – 4	›4	Chloroplast
	Chain	5 – 569	565	Ferredoxin--nitrite reductase, chloroplastic		PRO_0000019705
Sites
	Metal binding	447	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	453	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	488	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	492	1	Iron (siroheme axial ligand) By similarity
	Metal binding	492	1	Iron-sulfur (4Fe-4S) By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P17847 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: AA31738F2944CCE3	FASTA	569	63,342
	10 20 30 40 50 60 IPGRTGRARA AVSVPPPAGE QVPTERLEPR VEERAGGYWV LKEKYRAGLN PQEKVKLEKE 70 80 90 100 110 120 PMALFMEGGI QDLARVPMEQ IDAAKLTKDD VDVRLKWLGL FHRRKHQYGR FMMRLKLPNG 130 140 150 160 170 180 VTTSEQTRYL ASVIEAYGAD GCADVTTRQN WQIRGVTLPD VPAILDGLRA VGLTSLQSGM 190 200 210 220 230 240 DNVRNPVGNP LAGVDPHEIV DTRPYTNLLS SYVTNNSQGN PTITNLPRKW NVCVIGSHDL 250 260 270 280 290 300 YEHPHINDLA YMPAVKDGEF GFNLLVGGFI SPKRWAEALP LDAWVAGDDV VPVCKAILEA 310 320 330 340 350 360 YRDLGSRGNR QKTRMMWLID ELGMEVFRSE VEKRMPNGVL ERAAPEDLVD KRWERRDYLG 370 380 390 400 410 420 VHPQKQEGLS YVGLHVPVGR LQAADMFELA RLADEYGTGE LRLTVEQNIV LPNVSNERLD 430 440 450 460 470 480 ALLAEPLLQE QRLSPRPSML LRGLVACTGN QFCGQAIIET KARALQVARE VEKRVAVPRP 490 500 510 520 530 540 VRMHWTGCPN SCGQVQVADI GFMGCLTKDS DGKIVEAADI FVGGRVGSDS HLADVYRKSV 550 560 PCKDLVPIVA DLLVERFGAV PREREEDEE

References

[1]	"Molecular cloning of complementary DNA encoding maize nitrite reductase: molecular analysis and nitrate induction." Lahners K., Kramer V., Back E., Privalle L., Rothstein S. Plant Physiol. 88:741-746(1988) [PubMed: 16666376] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M23456 mRNA. Translation: AAA60450.1. Different initiation.
PIR	JA0172.
UniGene	Zm.13263.
3D structure databases
ProteinModelPortal	P17847.
SMR	P17847. Positions 26-562.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	P17847.
MaizeGDB	69266.
Phylogenomic databases
GeneTree	EPGT00070000030139.
Family and domain databases
InterPro	IPR005117. NiRdtase/SiRdtase_haem-b_fer. IPR006067. NO2/SO3_Rdtase_4Fe4S_dom. IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS. [Graphical view]
Gene3D	G3DSA:3.90.480.10. G3DSA:3.90.480.10. 2 hits.
Pfam	PF01077. NIR_SIR. 2 hits. PF03460. NIR_SIR_ferr. 2 hits. [Graphical view]
PRINTS	PR00397. SIROHAEM.
SUPFAM	SSF55124. NiR_SiRalpha_1/3. 2 hits.
PROSITE	PS00365. NIR_SIR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NIR_MAIZE

Accession

Primary (citable) accession number: P17847

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	November 16, 2011
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program