Reviewed, UniProtKB/Swiss-Prot P17845 (CYSI_SALTY)

Names and origin

Protein names

Recommended name:
    Sulfite reductase [NADPH] hemoprotein beta-component
      Short name=SiR-HP
      Short name=SiRHP
    EC=1.8.1.2

Gene names

Name:	cysI
Ordered Locus Names:	STM2947

Organism

Salmonella typhimurium [Complete proteome] [HAMAP]

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	570 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate. HAMAP MF_01540
Catalytic activity	H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH. HAMAP MF_01540
Cofactor	Binds 1 siroheme per subunit. HAMAP MF_01540 Binds 1 4Fe-4S cluster per subunit. HAMAP MF_01540
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1. HAMAP MF_01540
Subunit structure	Alpha(8)-beta8. The alpha component is a flavoprotein, the beta component is a hemoprotein. HAMAP MF_01540
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	4Fe-4S Heme Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation Inferred from electronic annotation. Source: HAMAP
Cellular component	sulfite reductase complex (NADPH) Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADP or NADPH binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro sulfite reductase (NADPH) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 570	569	Sulfite reductase [NADPH] hemoprotein beta-component HAMAP MF_01540		PRO_0000199908
Sites
	Metal binding	434	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	440	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	479	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	483	1	Iron (siroheme axial ligand) By similarity
	Metal binding	483	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P17845-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 62A9B26F077443C7	FASTA	570	64,023
	10 20 30 40 50 60 MSEKHPGPLV VEGKLSDAER MKLESNYLRG TIAEDLNDGL TGGFKGDNFL LIRFHGMYQQ 70 80 90 100 110 120 DDRDIRAERA AQKLEPRHAM LLRCRLPGGV ITTTQWQAID KFAADNTIYG SIRLTNRQTF 130 140 150 160 170 180 QFHGILKKNV KPVHQMLHSV GLDALATAND MNRNVLCTSN PYESQLHAEA YEWAKKISEH 190 200 210 220 230 240 LLPRTRAYAE IWLDQEKVAT TDEEPILGQT YLPRKFKTTV VIPPQNDIDL HANDMNFVAI 250 260 270 280 290 300 AENGKLVGFN LLVGGGLSIE HGNKKTYART ASEFGYLPLE HTLAVAEAVV TTQRDWGNRT 310 320 330 340 350 360 DRKNAKTKYT LERVGLETFK AEVERRAGIK FEPIRPYEFT GRGDRIGWVK GIDDKWHLTL 370 380 390 400 410 420 FIENGRILDY PGRPLKTGLL EIAKIHQGEF RITANQNLII ASVPESQKAK IETLARDHGL 430 440 450 460 470 480 MNAVKPQREN SMACVSFPTC PLAMAEAERF LPSFTDKVEA ILEKHGIPDE HIVMRVTGCP 490 500 510 520 530 540 NGCGRAMLAE IGLVGKAPGR YNLHLGGNRI GSRIPRMYKE NIAEPDILAS LDELIGRWAK 550 560 570 EREAGEGFGD FTVRAGIIRP VLDPARDFWE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the cysJIH regions of Salmonella typhimurium and Escherichia coli B. DNA sequences of cysI and cysH and a model for the siroheme-Fe4S4 active center of sulfite reductase hemoprotein based on amino acid homology with spinach nitrite reductase." Ostrowski J., Wu J.-Y., Rueger D.C., Miller B.E., Siegel L.M., Kredich N.M. J. Biol. Chem. 264:15726-15737(1989) [PubMed: 2670946] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LT2.
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M23007 Genomic DNA. Translation: AAA27047.1. AE006468 Genomic DNA. Translation: AAL21827.1.
PIR	A34354.
RefSeq	NP_461868.1.
3D structure databases
HSSP	HSSP built from PDB template 7GEP based on UniProtKB P17846.
SMR	P17845. Positions 81-570.
ModBase	Search...
Proteomic databases
PRIDE	P17845.
Genome annotation databases
GeneID	1254470.
GenomeReviews	Gene locus STM2947 in contig AE006468_GR.
KEGG	stm:STM2947.
NMPDR	fig|99287.1.peg.2843.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P17845.
OMA	ITTTQWQ.
Enzyme and pathway databases
BioCyc	STYP99287:STM2947-MON.
BRENDA	1.8.1.2. 2.
Family and domain databases
HAMAP	MF_01540. [Tree]
InterPro	IPR011786. CysI. IPR005117. NiRdtase/SiRdtase_haem-b_fer. IPR006067. NO2/SO3_Rdtase_4Fe4S. IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS. [Graphical view]
Pfam	PF01077. NIR_SIR. 1 hit. PF03460. NIR_SIR_ferr. 2 hits. [Graphical view]
PRINTS	PR00397. SIROHAEM.
TIGRFAMs	TIGR02041. CysI. 1 hit.
PROSITE	PS00365. NIR_SIR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSI_SALTY

Accession

Primary (citable) accession number: P17845

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 78 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)