Probable ATP-dependent RNA helicase DDX5

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Reviewed, UniProtKB/Swiss-Prot P17844 (DDX5_HUMAN)

Last modified July 7, 2009. Version 111. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Probable ATP-dependent RNA helicase DDX5
    EC=3.6.1.-
Alternative name(s):
    DEAD box protein 5
    RNA helicase p68

Gene names

Name:	DDX5
Synonyms:	G17P1, HELR, HLR1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	614 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	RNA-dependent ATPase activity. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. May be involved in pre-mRNA splicing.
Subunit structure	Identified in the spliceosome C complex, at least composed of AQR, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRPR, HNRPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRNP200, SNRNP40, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, XAB2 and ZCCHC8.
Subcellular location	Nucleus › nucleolus. Ref.2
Post-translational modification	Arg-502 is dimethylated, probably to asymmetric dimethylarginine. Ref.9
Sequence similarities	Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

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Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus Spliceosome
Coding sequence diversity	Polymorphism
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Helicase Hydrolase
PTM	Methylation Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	RNA splicing Inferred from electronic annotation. Source: UniProtKB-KW cell growth Ref.6 Non-traceable author statement. Source: UniProtKB mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell spliceosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: UniProtKB-KW RNA helicase activity Ref.2 Non-traceable author statement. Source: UniProtKB protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
NDRG1	Q92597	1	EBI-351962,EBI-716486
STK24	Q5T5B3	1	EBI-351962,EBI-1054809
TOB1	P50616	1	EBI-351962,EBI-723281

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 614

614

Probable ATP-dependent RNA helicase DDX5

PRO_0000054991

Regions

Domain

125 – 300

176

Helicase ATP-binding

Domain

328 – 475

148

Helicase C-terminal

Nucleotide binding

138 – 145

ATP By similarity

Motif

94 – 122

Q motif

Motif

248 – 251

DEAD box

Amino acid modifications

Modified residue

Phosphoserine Ref.12

Modified residue

202

Phosphotyrosine Ref.11

Modified residue

297

Phosphotyrosine Ref.10

Modified residue

480

Phosphoserine Ref.14 Ref.15

Modified residue

502

Omega-N-methylated arginine Ref.9

Modified residue

507

Phosphothreonine Ref.13

Modified residue

520

Phosphoserine Ref.13

Natural variations

Natural variant

480

S → A: dbSNP rs1140409.

VAR_029241

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Sequences

Sequence

Length

Mass (Da)

Tools

P17844-1 [UniParc].

Last modified August 1, 1990. Version 1.
Checksum: 84DF684FD6871594
Show »

FASTA

614

69,148

        10         20         30         40         50         60 
MSGYSSDRDR GRDRGFGAPR FGGSRAGPLS GKKFGNPGEK LVKKKWNLDE LPKFEKNFYQ 

        70         80         90        100        110        120 
EHPDLARRTA QEVETYRRSK EITVRGHNCP KPVLNFYEAN FPANVMDVIA RQNFTEPTAI 

       130        140        150        160        170        180 
QAQGWPVALS GLDMVGVAQT GSGKTLSYLL PAIVHINHQP FLERGDGPIC LVLAPTRELA 

       190        200        210        220        230        240 
QQVQQVAAEY CRACRLKSTC IYGGAPKGPQ IRDLERGVEI CIATPGRLID FLECGKTNLR 

       250        260        270        280        290        300 
RTTYLVLDEA DRMLDMGFEP QIRKIVDQIR PDRQTLMWSA TWPKEVRQLA EDFLKDYIHI 

       310        320        330        340        350        360 
NIGALELSAN HNILQIVDVC HDVEKDEKLI RLMEEIMSEK ENKTIVFVET KRRCDELTRK 

       370        380        390        400        410        420 
MRRDGWPAMG IHGDKSQQER DWVLNEFKHG KAPILIATDV ASRGLDVEDV KFVINYDYPN 

       430        440        450        460        470        480 
SSEDYIHRIG RTARSTKTGT AYTFFTPNNI KQVSDLISVL REANQAINPK LLQLVEDRGS 

       490        500        510        520        530        540 
GRSRGRGGMK DDRRDRYSAG KRGGFNTFRD RENYDRGYSS LLKRDFGAKT QNGVYSAANY 

       550        560        570        580        590        600 
TNGSFGSNFV SAGIQTSFRT GNPTGTYQNG YDSTQQYGSN VPNMHNGMNQ QAYAYPATAA 

       610 
APMIGYPMPT GYSQ

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete cDNA sequence of the human p68 protein." Hloch P., Stahl H. Nucleic Acids Res. 18:3045-3045(1990) [PubMed: 2349099] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"p68 RNA helicase: identification of a nucleolar form and cloning of related genes containing a conserved intron in yeasts." Iggo R.D., Jamieson D.J., McNeill S.A., Southgate J., McPheat J., Lane D.P. Mol. Cell. Biol. 11:1326-1333(1991) [PubMed: 1996094] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION.
[3]	"Structure and expression of the human p68 RNA helicase gene." Roessler O.G., Hloch P., Schutz N., Weitzenegger T., Stahl H. Nucleic Acids Res. 28:932-939(2000) [PubMed: 10648785] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Muscle.
[6]	"Nuclear protein with sequence homology to translation initiation factor eIF-4A." Ford M.J., Anton I.A., Lane D.P. Nature 332:736-738(1988) [PubMed: 2451786] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 21-614.
[7]	"Molecular organization of the murine p68 RNA helicase gene promotor region." Petry P., Bammer S., Heinlein U.A.O. Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 53-163.
[8]	"Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis." Jurica M.S., Licklider L.J., Gygi S.P., Grigorieff N., Moore M.J. RNA 8:426-439(2002) [PubMed: 11991638] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SPICEOSOMAL C COMPLEX.
[9]	"Identifying and quantifying in vivo methylation sites by heavy methyl SILAC." Ong S.E., Mittler G., Mann M. Nat. Methods 1:119-126(2004) [PubMed: 15782174] [Abstract] Cited for: METHYLATION [LARGE SCALE ANALYSIS] AT ARG-502, MASS SPECTROMETRY.
[10]	"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J. Nat. Biotechnol. 23:94-101(2005) [PubMed: 15592455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-297, MASS SPECTROMETRY.
[11]	"Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer." Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J., Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L., Mitchell J., Wetzel R., Macneill J., Ren J.M. Comb M.J. Cell 131:1190-1203(2007) [PubMed: 18083107] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-202, MASS SPECTROMETRY.
[12]	"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed: 17924679] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-30, MASS SPECTROMETRY. Tissue: Epithelium.
[13]	"Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry." Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A. Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007) [PubMed: 17287340] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-507 AND SER-520, MASS SPECTROMETRY.
[14]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-480, MASS SPECTROMETRY.
[15]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-480, MASS SPECTROMETRY.
[16]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

X52104 mRNA. Translation: CAA36324.1.
AF015812 Genomic DNA. Translation: AAB84094.1.
BT006943 mRNA. Translation: AAP35589.1.
BC016027 mRNA. Translation: AAH16027.1.
X15729 mRNA. Translation: CAA33751.1.
AJ010931 Genomic DNA. Translation: CAA09408.1.

IPI

IPI00017617.

PIR

JC1087.

RefSeq

NP_004387.1.

UniGene

Hs.279806

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3FE2	X-ray	2.60	A/B	68-307	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P17844. 22 interactions.

PTM databases

PhosphoSite

P17844.

2-D gel databases

SWISS-2DPAGE

P17844.

Aarhus/Ghent-2DPAGE

1605. NEPHGE.

Proteomic databases

PeptideAtlas

P17844.

PRIDE

P17844.

Genome annotation databases

Ensembl

ENSG00000108654. Homo sapiens. [Contig view]

GeneID

1655.

KEGG

hsa:1655.

UCSC

uc002jek.1. human.

Organism-specific databases

GeneCards

GC17M059926.

HGNC

HGNC:2746. DDX5.

HPA

CAB005868.

MIM

180630. gene.

PharmGKB

PA27228.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

P17844.

HOVERGEN

P17844.

OMA

P17844. TIDAMSG.

Gene expression databases

ArrayExpress

P17844.

Bgee

P17844.

CleanEx

HS_DDX5.

GermOnline

ENSG00000108654. Homo sapiens.

Family and domain databases

InterPro

IPR014001. DEAD-like_N.
IPR001650. DNA/RNA_helicase_C.
IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014021. Helicase_SF1/SF2_ATP-bd.
IPR012587. P68HR.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]

Pfam

PF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF08061. P68HR. 2 hits.
[Graphical view]

SMART

SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]

PROSITE

PS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

6816.

SOURCE

Search...

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Entry information

Entry name

DDX5_HUMAN

Accession

Primary (citable) accession number: P17844
Secondary accession number(s): O75681, Q53Y61

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	July 7, 2009
This is version 111 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

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Human chromosome 17: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

2-D gel databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents