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Protein

Floral homeotic protein AGAMOUS

Gene

AG

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable transcription factor involved in the control of organ identity during the early development of flowers. Is required for normal development of stamens and carpels in the wild-type flower. Plays a role in maintaining the determinacy of the floral meristem. Acts as C class cadastral protein by repressing the A class floral homeotic genes like APETALA1. Forms a heterodimer via the K-box domain with either SEPALATTA1/AGL2, SEPALATTA2/AGL4, SEPALLATA3/AGL9 or AGL6 that could be involved in genes regulation during floral meristem development. Controls AHL21/GIK, a multifunctional chromatin modifier in reproductive organ patterning and differentiation (PubMed:19956801). Induces microsporogenesis through the activation of SPL/NZZ (PubMed:15254538).2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Differentiation, Flowering, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Floral homeotic protein AGAMOUS
Gene namesi
Name:AG
Ordered Locus Names:At4g18960
ORF Names:F13C5.130
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G18960.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mutations result in the replacement of the six stamens by six petals and of the carpels by a new mutant flower.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 252252Floral homeotic protein AGAMOUSPRO_0000199445Add
BLAST

Proteomic databases

PaxDbiP17839.

Expressioni

Tissue specificityi

Detected early in the floral meristem but mostly expressed in stamen and carpel primordia.1 Publication

Inductioni

Negatively regulated by the A class floral homeotic protein APETALA2 and by other repressors like LEUNIG, SEUSS, SAP or CURLY LEAF. Positively regulated by both LEAFY and APETALA1. Repressed by silencing mediated by polycomb group (PcG) protein complex containing EMF1 and EMF2. Up-regulated by HUA2.7 Publications

Gene expression databases

ExpressionAtlasiP17839. baseline and differential.
GenevisibleiP17839. AT.

Interactioni

Subunit structurei

Homodimer, capable of binding to CArG-box sequences. Forms a heterodimer via the K-box domain with either SEPALATTA1/AGL2, SEPALATTA2/AGL4, SEPALLATA3/AGL9 or AGL6. Heterodimerization also seen with some other Agamous-like MADS-box proteins. Interacts with AGL15 and AGL16.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
AGL6Q1PEU33EBI-592365,EBI-2315134
At1g24260B4F7R93EBI-592365,EBI-2315153
SEP1Q5XXN83EBI-592365,EBI-2315096
SEP3O224564EBI-592365,EBI-592020

Protein-protein interaction databases

BioGridi12924. 29 interactions.
IntActiP17839. 23 interactions.
STRINGi3702.AT4G18960.1.

Structurei

3D structure databases

ProteinModelPortaliP17839.
SMRiP17839. Positions 19-74, 107-189.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini19 – 7355MADS-boxPROSITE-ProRule annotationAdd
BLAST
Domaini103 – 19391K-boxPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili99 – 16668Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 K-box domain.PROSITE-ProRule annotation
Contains 1 MADS-box domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0014. Eukaryota.
COG5068. LUCA.
HOGENOMiHOG000155301.
InParanoidiP17839.
PhylomeDBiP17839.

Family and domain databases

InterProiIPR002487. TF_Kbox.
IPR002100. TF_MADSbox.
[Graphical view]
PfamiPF01486. K-box. 1 hit.
PF00319. SRF-TF. 1 hit.
[Graphical view]
PRINTSiPR00404. MADSDOMAIN.
SMARTiSM00432. MADS. 1 hit.
[Graphical view]
SUPFAMiSSF55455. SSF55455. 1 hit.
PROSITEiPS51297. K_BOX. 1 hit.
PS00350. MADS_BOX_1. 1 hit.
PS50066. MADS_BOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P17839-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAYQSELGGD SSPLRKSGRG KIEIKRIENT TNRQVTFCKR RNGLLKKAYE
60 70 80 90 100
LSVLCDAEVA LIVFSSRGRL YEYSNNSVKG TIERYKKAIS DNSNTGSVAE
110 120 130 140 150
INAQYYQQES AKLRQQIISI QNSNRQLMGE TIGSMSPKEL RNLEGRLERS
160 170 180 190 200
ITRIRSKKNE LLFSEIDYMQ KREVDLHNDN QILRAKIAEN ERNNPSISLM
210 220 230 240 250
PGGSNYEQLM PPPQTQSQPF DSRNYFQVAA LQPNNHHYSS AGRQDQTALQ

LV
Length:252
Mass (Da):28,723
Last modified:December 15, 1998 - v2
Checksum:i4E7591AD85654C1C
GO

Sequence cautioni

The sequence CAA16753.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAA37642.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAB78898.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 11M → T in CAA37642 (PubMed:1973265).Curated
Sequence conflicti1 – 11M → T in CAA16753 (PubMed:1973265).Curated
Sequence conflicti1 – 11M → T in CAB78898 (PubMed:1973265).Curated
Sequence conflicti1 – 11M → T in AEE84111 (PubMed:10617198).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53579 mRNA. Translation: CAA37642.1. Different initiation.
AL021711 Genomic DNA. Translation: CAA16753.1. Sequence problems.
AL161549 Genomic DNA. Translation: CAB78898.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE84111.1.
PIRiA85214.
T05033.
RefSeqiNP_567569.3. NM_118013.2.
UniGeneiAt.28088.

Genome annotation databases

EnsemblPlantsiAT4G18960.1; AT4G18960.1; AT4G18960.
GeneIDi827631.
GrameneiAT4G18960.1; AT4G18960.1; AT4G18960.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53579 mRNA. Translation: CAA37642.1. Different initiation.
AL021711 Genomic DNA. Translation: CAA16753.1. Sequence problems.
AL161549 Genomic DNA. Translation: CAB78898.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE84111.1.
PIRiA85214.
T05033.
RefSeqiNP_567569.3. NM_118013.2.
UniGeneiAt.28088.

3D structure databases

ProteinModelPortaliP17839.
SMRiP17839. Positions 19-74, 107-189.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi12924. 29 interactions.
IntActiP17839. 23 interactions.
STRINGi3702.AT4G18960.1.

Proteomic databases

PaxDbiP17839.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G18960.1; AT4G18960.1; AT4G18960.
GeneIDi827631.
GrameneiAT4G18960.1; AT4G18960.1; AT4G18960.

Organism-specific databases

TAIRiAT4G18960.

Phylogenomic databases

eggNOGiKOG0014. Eukaryota.
COG5068. LUCA.
HOGENOMiHOG000155301.
InParanoidiP17839.
PhylomeDBiP17839.

Miscellaneous databases

PROiP17839.

Gene expression databases

ExpressionAtlasiP17839. baseline and differential.
GenevisibleiP17839. AT.

Family and domain databases

InterProiIPR002487. TF_Kbox.
IPR002100. TF_MADSbox.
[Graphical view]
PfamiPF01486. K-box. 1 hit.
PF00319. SRF-TF. 1 hit.
[Graphical view]
PRINTSiPR00404. MADSDOMAIN.
SMARTiSM00432. MADS. 1 hit.
[Graphical view]
SUPFAMiSSF55455. SSF55455. 1 hit.
PROSITEiPS51297. K_BOX. 1 hit.
PS00350. MADS_BOX_1. 1 hit.
PS50066. MADS_BOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The protein encoded by the Arabidopsis homeotic gene agamous resembles transcription factors."
    Yanofsky M.F., Ma H., Bowman J.L., Drews G., Feldmann K.A., Meyerowitz E.M.
    Nature 346:35-39(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DISRUPTION PHENOTYPE.
    Strain: cv. Landsberg erecta.
  2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Negative regulation of the Arabidopsis homeotic gene AGAMOUS by the APETALA2 product."
    Drews G.N., Bowman J.L., Meyerowitz E.M.
    Cell 65:991-1002(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INDUCTION.
  5. "Activation of floral homeotic genes in Arabidopsis."
    Weigel D., Meyerowitz E.M.
    Science 261:1723-1726(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  6. "The ABCs of floral homeotic genes."
    Weigel D., Meyerowitz E.M.
    Cell 78:203-209(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  7. "Dimerization specificity of Arabidopsis MADS domain homeotic proteins APETALA1, APETALA3, PISTILLATA, and AGAMOUS."
    Riechmann J.L., Krizek B.A., Meyerowitz E.M.
    Proc. Natl. Acad. Sci. U.S.A. 93:4793-4798(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  8. "Specific interactions between the K domains of AG and AGLs, members of the MADS domain family of DNA binding proteins."
    Fan H.-Y., Hu Y., Tudor M., Ma H.
    Plant J. 12:999-1010(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  9. "A genetic framework for floral patterning."
    Parcy F., Nilsson O., Busch M.A., Lee I., Weigel D.
    Nature 395:561-566(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  10. "HUA1 and HUA2 are two members of the floral homeotic AGAMOUS pathway."
    Chen X., Meyerowitz E.M.
    Mol. Cell 3:349-360(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY HUA2.
    Strain: cv. Columbia.
  11. "LEUNIG, a putative transcriptional corepressor that regulates AGAMOUS expression during flower development."
    Conner J., Liu Z.
    Proc. Natl. Acad. Sci. U.S.A. 97:12902-12907(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  12. "The homeotic protein AGAMOUS controls microsporogenesis by regulation of SPOROCYTELESS."
    Ito T., Wellmer F., Yu H., Das P., Ito N., Alves-Ferreira M., Riechmann J.L., Meyerowitz E.M.
    Nature 430:356-360(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  13. Cited for: INTERACTION WITH AGL15 AND AGL16.
  14. "EMBRYONIC FLOWER1 participates in polycomb group-mediated AG gene silencing in Arabidopsis."
    Calonje M., Sanchez R., Chen L., Sung Z.R.
    Plant Cell 20:277-291(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY EMF1.
  15. "AGAMOUS controls GIANT KILLER, a multifunctional chromatin modifier in reproductive organ patterning and differentiation."
    Ng K.H., Yu H., Ito T.
    PLoS Biol. 7:E1000251-E1000251(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  16. "Epigenetic regulation of gene programs by EMF1 and EMF2 in Arabidopsis."
    Kim S.Y., Zhu T., Sung Z.R.
    Plant Physiol. 152:516-528(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY EMF1 AND EMF2.

Entry informationi

Entry nameiAG_ARATH
AccessioniPrimary (citable) accession number: P17839
Secondary accession number(s): O82732
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: December 15, 1998
Last modified: April 13, 2016
This is version 154 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.