Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Chaperone protein DnaK

Gene

dnaK

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a chaperone.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU25470-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein DnaK
Alternative name(s):
HSP70
Heat shock 70 kDa protein
Heat shock protein 70
Gene namesi
Name:dnaK
Ordered Locus Names:BSU25470
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 611610Chaperone protein DnaKPRO_0000078420Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei173 – 1731Phosphothreonine; by autocatalysisBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP17820.
PRIDEiP17820.

Expressioni

Inductioni

By heat shock.

Interactioni

Protein-protein interaction databases

IntActiP17820. 3 interactions.
MINTiMINT-8364924.
STRINGi224308.Bsubs1_010100013931.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FZOmodel-A1-611[»]
ProteinModelPortaliP17820.
SMRiP17820. Positions 1-577.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the heat shock protein 70 family.Curated

Phylogenomic databases

eggNOGiENOG4105CFG. Bacteria.
COG0443. LUCA.
HOGENOMiHOG000228137.
InParanoidiP17820.
KOiK04043.
OMAiEMMYKDA.
OrthoDBiEOG6JMMSV.
PhylomeDBiP17820.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
HAMAPiMF_00332. DnaK.
InterProiIPR012725. Chaperone_DnaK.
IPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 2 hits.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
TIGRFAMsiTIGR02350. prok_dnaK. 1 hit.
PROSITEiPS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17820-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKVIGIDLG TTNSCVAVLE GGEPKVIANA EGNRTTPSVV AFKNGERQVG
60 70 80 90 100
EVAKRQSITN PNTIMSIKRH MGTDYKVEIE GKDYTPQEVS AIILQHLKSY
110 120 130 140 150
AESYLGETVS KAVITVPAYF NDAERQATKD AGKIAGLEVE RIINEPTAAA
160 170 180 190 200
LAYGLDKTDE DQTILVYDLG GGTFDVSILE LGDGVFEVRS TAGDNRLGGD
210 220 230 240 250
DFDQVIIDHL VSEFKKENGI DLSKDKMALQ RLKDAAEKAK KDLSGVSSTQ
260 270 280 290 300
ISLPFITAGE AGPLHLELTL TRAKFEELSS HLVERTMGPV RQALQDAGLS
310 320 330 340 350
ASEIDKVILV GGSTRIPAVQ EAIKKETGKE AHKGVNPDEV VALGAAIQGG
360 370 380 390 400
VITGDVKDVV LLDVTPLSLG IETMGGVFTK LIDRNTTIPT SKSQVFSTAA
410 420 430 440 450
DNQTAVDIHV LQGERPMSAD NKTLGRFQLT DIPPAPRGVP QIEVSFDIDK
460 470 480 490 500
NGIVNVRAKD LGTGKEQNIT IKSSSGLSDE EIERMVKEAE ENADADAKKK
510 520 530 540 550
EEIEVRNEAD QLVFQTEKTL KDLEGKVDEE QVKKANDAKD ALKAAIEKNE
560 570 580 590 600
FEEIKAKKDE LQTIVQELSM KLYEEAAKAQ QAQGGANAEG KADDNVVDAE
610
YEEVNDDQNK K
Length:611
Mass (Da):66,002
Last modified:January 23, 2007 - v3
Checksum:iE83CEA6E16A31593
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti99 – 991S → R in CAA34429 (PubMed:2510131).Curated
Sequence conflicti150 – 1501A → P in CAA34429 (PubMed:2510131).Curated
Sequence conflicti166 – 1661V → L in CAA34429 (PubMed:2510131).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52064 Genomic DNA. Translation: CAA36286.1.
M84964 Genomic DNA. Translation: AAA22528.1.
D84432 Genomic DNA. Translation: BAA12464.1.
AL009126 Genomic DNA. Translation: CAB14489.1.
X51477 Genomic DNA. Translation: CAA35842.1.
X16393 Genomic DNA. Translation: CAA34429.1.
PIRiS09500.
RefSeqiNP_390425.1. NC_000964.3.
WP_004398786.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB14489; CAB14489; BSU25470.
GeneIDi937849.
KEGGibsu:BSU25470.
PATRICi18976932. VBIBacSub10457_2655.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52064 Genomic DNA. Translation: CAA36286.1.
M84964 Genomic DNA. Translation: AAA22528.1.
D84432 Genomic DNA. Translation: BAA12464.1.
AL009126 Genomic DNA. Translation: CAB14489.1.
X51477 Genomic DNA. Translation: CAA35842.1.
X16393 Genomic DNA. Translation: CAA34429.1.
PIRiS09500.
RefSeqiNP_390425.1. NC_000964.3.
WP_004398786.1. NZ_JNCM01000036.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FZOmodel-A1-611[»]
ProteinModelPortaliP17820.
SMRiP17820. Positions 1-577.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP17820. 3 interactions.
MINTiMINT-8364924.
STRINGi224308.Bsubs1_010100013931.

Proteomic databases

PaxDbiP17820.
PRIDEiP17820.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14489; CAB14489; BSU25470.
GeneIDi937849.
KEGGibsu:BSU25470.
PATRICi18976932. VBIBacSub10457_2655.

Phylogenomic databases

eggNOGiENOG4105CFG. Bacteria.
COG0443. LUCA.
HOGENOMiHOG000228137.
InParanoidiP17820.
KOiK04043.
OMAiEMMYKDA.
OrthoDBiEOG6JMMSV.
PhylomeDBiP17820.

Enzyme and pathway databases

BioCyciBSUB:BSU25470-MONOMER.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
HAMAPiMF_00332. DnaK.
InterProiIPR012725. Chaperone_DnaK.
IPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 2 hits.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
TIGRFAMsiTIGR02350. prok_dnaK. 1 hit.
PROSITEiPS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete nucleotide sequence of the Bacillus subtilis dnaK gene."
    Wetzstein M., Schumann W.
    Nucleic Acids Res. 18:2172-2172(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168 / MB11.
  2. "Cloning, sequencing, and molecular analysis of the dnaK locus from Bacillus subtilis."
    Wetzstein M., Voelker U., Dedio J., Loebau S., Zuber U., Schiesswohl M., Herget C., Hecker M., Schumann W.
    J. Bacteriol. 174:3300-3310(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168 / MB11.
  3. "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes."
    Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y.
    Microbiology 142:3103-3111(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168 / JH642.
  4. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  5. "Nucleotide sequence of a Bacillus subtilis gene homologous to the grpE gene of E. coli located immediately upstream of the dnaK gene."
    Wetzstein M., Schumann W.
    Nucleic Acids Res. 18:1289-1289(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-572.
    Strain: 168 / MB11.
  6. "Nucleotide sequence of a Bacillus subtilis gene homologous to the dnaK gene of Escherichia coli."
    Hearne C.M., Ellar D.J.
    Nucleic Acids Res. 17:8373-8373(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-214.
    Strain: 168 / MB24.
  7. "Analysis of the induction of general stress proteins of Bacillus subtilis."
    Voelker U., Engelmann S., Maul B., Riethdorf S., Voelker A., Schmid R., Mach H., Hecker M.
    Microbiology 140:741-752(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-8.
    Strain: 168 / IS58.

Entry informationi

Entry nameiDNAK_BACSU
AccessioniPrimary (citable) accession number: P17820
Secondary accession number(s): P13343
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: January 23, 2007
Last modified: February 17, 2016
This is version 132 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.