Reviewed, UniProtKB/Swiss-Prot P17819 (G3P1_KLULA)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase 1
      Short name=GAPDH 1
    EC=1.2.1.12

Gene names

Name:	GAP1
Ordered Locus Names:	KLLA0F20988g

Organism

Kluyveromyces lactis (Yeast) (Candida sphaerica) [Complete proteome]

Taxonomic identifier

28985 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Kluyveromyces

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 329

329

Glyceraldehyde-3-phosphate dehydrogenase 1

PRO_0000145556

Regions

Nucleotide binding

11 – 12

2

NAD By similarity

Region

148 – 150

3

Glyceraldehyde 3-phosphate binding By similarity

Region

208 – 209

2

Glyceraldehyde 3-phosphate binding By similarity

Sites

Active site

149

1

Nucleophile By similarity

Binding site

33

1

NAD By similarity

Binding site

78

1

NAD; via carbonyl oxygen By similarity

Binding site

179

1

Glyceraldehyde 3-phosphate By similarity

Binding site

231

1

Glyceraldehyde 3-phosphate By similarity

Binding site

313

1

NAD By similarity

Site

176

1

Activates thiol group during catalysis By similarity

Secondary structure

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329

Helix Strand Turn

Details...

Beta strand

5 – 7

3

Helix

11 – 20

10

Beta strand

26 – 32

7

Helix

38 – 46

9

Turn

49 – 51

3

Beta strand

58 – 60

3

Beta strand

62 – 67

6

Beta strand

70 – 75

6

Helix

80 – 82

3

Turn

85 – 89

5

Beta strand

91 – 95

5

Beta strand

97 – 100

4

Helix

103 – 111

9

Beta strand

115 – 121

7

Beta strand

124 – 126

3

Turn

131 – 133

3

Helix

135 – 137

3

Beta strand

142 – 145

4

Helix

149 – 164

16

Beta strand

167 – 176

10

Beta strand

204 – 207

4

Turn

210 – 213

4

Helix

214 – 217

4

Helix

219 – 221

3

Beta strand

224 – 231

8

Beta strand

236 – 248

13

Helix

252 – 264

13

Turn

265 – 270

6

Beta strand

271 – 274

4

Helix

280 – 283

4

Beta strand

288 – 293

6

Turn

294 – 296

3

Beta strand

298 – 301

4

Beta strand

304 – 314

11

Helix

315 – 328

14

Sequences

	Sequence		Length	Mass (Da)
	P17819-1 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: EDE4F68FEDC86933	FASTA	329	35,324
	10 20 30 40 50 60 MVKVAINGFG RIGRLVLRIA LQRKALEVVA VNDPFISVDY AAYMFKYDST HGRYKGEVTT 70 80 90 100 110 120 SGNDLVIDGH KIAVFQEKDP ANLPWGKLGV DIVIDSTGVF KELDSAQKHL DAGAKKVVIT 130 140 150 160 170 180 APSKTAPMFV VGVNEDKYNG ETIVSNASCT TNCLAPIAKI INDEFGIDEA LMTTVHSITA 190 200 210 220 230 240 TQKTVDGPSH KDWRGGRTAS GNIIPSSTGA AKAVGKVLPE LQGKLTGMAF RVPTVDVSVV 250 260 270 280 290 300 DLTVKLAKEA TYDEIKAAVK KASQGKLKNV VGYTEDSVVS SDFLGDTHST IFDASAGIQL 310 320 SPKFVKVVAW YDNEYGYSER VVDLVEHVA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Kluyveromyces lactis glyceraldehyde-3-phosphate dehydrogenase and alcohol dehydrogenase-1 genes are linked and divergently transcribed." Shuster J.R. Nucleic Acids Res. 18:4271-4271(1990) [PubMed: 2377477] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SD11.
[2]	"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NRRL Y-1140 / WM37.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X52871 Genomic DNA. Translation: CAA37051.1.
CR382126 Genomic DNA. Translation: CAG98730.1.

PIR

DEVKGL. S12721.

RefSeq

XP_456022.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2I5P	X-ray	2.30	O	5-329	[»]

ModBase

Search...

Genome annotation databases

GeneID

2894950.

KEGG

kla:KLLA0F20988g.

Phylogenomic databases

HOGENOM

P17819.

OMA

P17819. WGETGAE.

Enzyme and pathway databases

BRENDA

1.2.1.12. 74088.

Family and domain databases

InterPro

IPR000173. GlycerAld_3-P_DH.
IPR006424. Glyceraldehyde-3-P_DH_1.
[Graphical view]

PANTHER

PTHR10836. GAP_DH. 1 hit.

Pfam

PF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]

PIRSF

PIRSF000149. GAP_DH. 1 hit.

PRINTS

PR00078. G3PDHDRGNASE.

TIGRFAMs

TIGR01534. GAPDH-I. 1 hit.

PROSITE

PS00071. GAPDH. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

G3P1_KLULA

Accession

Primary (citable) accession number: P17819

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	June 16, 2009
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)