ID   CHSY_MATIN              Reviewed;         394 AA.
AC   P17818;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 1.
DT   22-FEB-2023, entry version 95.
DE   RecName: Full=Chalcone synthase;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase;
GN   Name=CHS;
OS   Matthiola incana (Common stock) (Cheiranthus incanus).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Anchonieae; Matthiola.
OX   NCBI_TaxID=3724;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Petal;
RX   PubMed=2102873; DOI=10.1007/bf00019405;
RA   Epping B., Kittel M., Ruhnau B., Hemleben V.;
RT   "Isolation and sequence analysis of a chalcone synthase cDNA of Matthiola
RT   incana R. Br. (Brassicaceae).";
RL   Plant Mol. Biol. 14:1061-1063(1990).
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; X17577; CAA35600.1; -; mRNA.
DR   PIR; S11876; SYJCCS.
DR   AlphaFoldDB; P17818; -.
DR   SMR; P17818; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd00831; CHS_like; 1.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877:SF14; CHALCONE SYNTHASE; 1.
DR   PANTHER; PTHR11877; HYDROXYMETHYLGLUTARYL-COA SYNTHASE; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; Thiolase-like; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   2: Evidence at transcript level;
KW   Acetylation; Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250|UniProtKB:P13114"
FT   CHAIN           2..394
FT                   /note="Chalcone synthase"
FT                   /id="PRO_0000216005"
FT   ACT_SITE        168
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
FT   MOD_RES         2
FT                   /note="N-acetylvaline"
FT                   /evidence="ECO:0000250|UniProtKB:P13114"
SQ   SEQUENCE   394 AA;  42969 MW;  EF2DA6C0FB550714 CRC64;
     MVMGATSLDE IRKAQRADGP AGILGIGTAN PANHVIQAEY PDYYFRITNS EHMTDLKEKF
     QRMCDKSMIR KRHMHLTEDF LKENPNMCAY MAPSLDARQD IVVVEVPKLG KEAAVRAIKE
     WGQPKSKITH LVFCTTSGVD MPGADYQLTK LLGLRPSVKR LMMYQQGCFA GGTVLRLAKD
     LAENNRGARV LVVCSEITAV TFRGPSDTHL DSLVGQALFS DGAAALIVGS DPDTSVGEKP
     IFEMVSAAQT ILPDSDGAID GHLREVGLTF HLLKDVPGLI SKNIEKSLEE AFKPLGISDW
     NSLFWIAHPG GPAILDQVEI KLGLKAEKMR ATRHVLSEYG NMSSACVLFI LDEMRKKSAQ
     DGVATTGEGL EWGVLFGFGP GLTVETVVLR SVPL
//