ID   4CL1_ORYSJ              Reviewed;         564 AA.
AC   P17814; Q0J6Z8; Q6ZKV9;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   27-SEP-2005, sequence version 2.
DT   14-DEC-2011, entry version 80.
DE   RecName: Full=Probable 4-coumarate--CoA ligase 1;
DE            Short=4CL 1;
DE            Short=Os4CL1;
DE            EC=6.2.1.12;
DE   AltName: Full=4-coumaroyl-CoA synthase 1;
GN   Name=4CL1; Synonyms=4CL;
GN   OrderedLocusNames=Os08g0245200, LOC_Os08g14760;
GN   ORFNames=OJ1033_B09.16;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade;
OC   Ehrhartoideae; Oryzeae; Oryza.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=91045096; PubMed=2235510; DOI=10.1093/nar/18.20.6144;
RA   Zhao Y., Kung S.D., Dube S.K.;
RT   "Nucleotide sequence of rice 4-coumarate:CoA ligase gene, 4-CL.1.";
RL   Nucleic Acids Res. 18:6144-6144(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   MEDLINE=22752273; PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [5]
RP   GENE FAMILY.
RX   PubMed=18627494; DOI=10.1111/j.1469-8137.2008.02534.x;
RA   de Azevedo Souza C., Barbazuk B., Ralph S.G., Bohlmann J.,
RA   Hamberger B., Douglas C.J.;
RT   "Genome-wide analysis of a land plant-specific acyl:coenzyme A
RT   synthetase (ACS) gene family in Arabidopsis, poplar, rice and
RT   Physcomitrella.";
RL   New Phytol. 179:987-1003(2008).
CC   -!- CATALYTIC ACTIVITY: ATP + 4-coumarate + CoA = AMP + diphosphate +
CC       4-coumaroyl-CoA.
CC   -!- PATHWAY: Phytoalexin biosynthesis; 3,4',5-trihydroxystilbene
CC       biosynthesis; 3,4',5-trihydroxystilbene from trans-4-coumarate:
CC       step 1/2.
CC   -!- INDUCTION: By fungal elicitor and UV irradiation.
CC   -!- DOMAIN: Both substrate-binding domains (SBD1 and SBD2) are
CC       involved in the substrate recognition, and are sufficient to
CC       confer the substrate specificity.
CC   -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme
CC       family.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAD05189.1; Type=Erroneous initiation;
CC       Sequence=CAA36850.1; Type=Erroneous gene model prediction;
CC       Sequence=CAA36850.1; Type=Frameshift; Positions=496;
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DR   EMBL; X52623; CAA36850.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP003859; BAD05189.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AP008214; BAF23267.1; -; Genomic_DNA.
DR   EMBL; AK069932; -; NOT_ANNOTATED_CDS; mRNA.
DR   PIR; JU0311; JU0311.
DR   RefSeq; NP_001061353.1; NM_001067888.1.
DR   UniGene; Os.52921; -.
DR   ProteinModelPortal; P17814; -.
DR   STRING; P17814; -.
DR   EnsemblPlants; LOC_Os08g14760.1; LOC_Os08g14760.1; LOC_Os08g14760.
DR   GeneID; 4345054; -.
DR   KEGG; osa:4345054; -.
DR   Gramene; P17814; -.
DR   GeneTree; EPGT00070000028108; -.
DR   HOGENOM; HBG547964; -.
DR   OMA; LITHPSI; -.
DR   PhylomeDB; P17814; -.
DR   ProtClustDB; PLN02246; -.
DR   GO; GO:0016207; F:4-coumarate-CoA ligase activity; IEA:EC.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0009698; P:phenylpropanoid metabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR020845; AMP-binding_CS.
DR   InterPro; IPR000873; AMP-dep_Synth/Lig.
DR   KO; K01904; -.
DR   Pfam; PF00501; AMP-binding; 1.
DR   PROSITE; PS00455; AMP_BINDING; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Ligase; Nucleotide-binding; Phenylpropanoid metabolism.
FT   CHAIN         1    564       Probable 4-coumarate--CoA ligase 1.
FT                                /FTId=PRO_0000193031.
FT   NP_BIND     209    217       ATP (By similarity).
FT   NP_BIND     352    357       ATP (By similarity).
FT   REGION      282    351       SBD1.
FT   REGION      352    419       SBD2.
FT   BINDING     440    440       ATP (By similarity).
FT   BINDING     455    455       ATP (By similarity).
FT   BINDING     547    547       ATP (By similarity).
FT   CONFLICT    405    405       P -> R (in Ref. 1; CAA36850).
FT   CONFLICT    477    477       I -> N (in Ref. 1; CAA36850).
SQ   SEQUENCE   564 AA;  60902 MW;  9725A4B4A72F8C6E CRC64;
     MGSMEQQQPE SAAPATEASP EIIFRSKLQD IAITNTLPLH RYCFERLPEV AARPCLIDGA
     TGGVLTYADV DRLSRRLAAA LRRAPLGLRR GGVVMSLLRN SPEFVLSFFA ASRVGAAVTT
     ANPMSTPHEI ESQLAAAGAT VVITESMAAD KLPSHSHGAL TVVLIDERRD GCLHFWDDLM
     SEDEASPLAG DEDDEKVFDP DDVVALPYSS GTTGLPKGVM LTHRSLSTSV AQQVDGENPN
     IGLHAGDVIL CALPMFHIYS LNTIMMCGLR VGAAIVVMRR FDLAAMMDLV ERHRVTIAPL
     VPPIVVAVAK SEAAAARDLS SVRMVLSGAA PMGKDIEDAF MAKLPGAVLG QGYGMTEAGP
     VLSMCLAFAK EPFKVKSGAC GTVVRNAELK IIDPDTGKSL GRNLPGEICI RGQQIMKGYL
     NNPEATKNTI DAEGWLHTGD IGYVDDDDEI FIVDRLKEII KYRGFQVAPA ELEALLITHP
     SIADAAVVGK QIEPEIGEIP VAFVAKTEGS ELSEDDVKQF VAKEVIYYKK IREVFFVDKI
     PKAPSGKILR KELRKQLQHL QQEA
//