Reviewed, UniProtKB/Swiss-Prot P17814 (4CL1_ORYSJ)

Names and origin

Protein names

Recommended name:
    Probable 4-coumarate--CoA ligase 1
      Short name=4CL 1
      Short name=Os4CL1
    EC=6.2.1.12
Alternative name(s):
    4-coumaroyl-CoA synthase 1

Gene names

Name:	4CL1
Synonyms:	4CL
Ordered Locus Names:	Os08g0245200, LOC_Os08g14760
ORF Names:	OJ1033_B09.16

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	564 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + 4-coumarate + CoA = AMP + diphosphate + 4-coumaroyl-CoA.
Pathway	Phytoalexin biosynthesis; 3,4',5-trihydroxystilbene biosynthesis; 3,4',5-trihydroxystilbene from trans-4-coumarate: step 1/2.
Induction	By fungal elicitor and UV irradiation.
Domain	Both substrate-binding domains (SBD1 and SBD2) are involved in the substrate recognition, and are sufficient to confer the substrate specificity.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.
Sequence caution	The sequence CAA36850.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAA36850.1 differs from that shown. Reason: Frameshift at position 496.

Ontologies

Keywords
Biological process	Phenylpropanoid metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	phenylpropanoid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4-coumarate-CoA ligase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 564	564	Probable 4-coumarate--CoA ligase 1		PRO_0000193031
Regions
	Nucleotide binding	209 – 217	9	ATP By similarity
	Nucleotide binding	352 – 357	6	ATP By similarity
	Region	282 – 351	70	SBD1
	Region	352 – 419	68	SBD2
Sites
	Binding site	440	1	ATP By similarity
	Binding site	455	1	ATP By similarity
	Binding site	547	1	ATP By similarity
Experimental info
	Sequence conflict	405	1	P → R in CAA36850. Ref.1
	Sequence conflict	477	1	I → N in CAA36850. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P17814-1 [UniParc]. Last modified September 27, 2005. Version 2. Checksum: 9725A4B4A72F8C6E	FASTA	564	60,902
	10 20 30 40 50 60 MGSMEQQQPE SAAPATEASP EIIFRSKLQD IAITNTLPLH RYCFERLPEV AARPCLIDGA 70 80 90 100 110 120 TGGVLTYADV DRLSRRLAAA LRRAPLGLRR GGVVMSLLRN SPEFVLSFFA ASRVGAAVTT 130 140 150 160 170 180 ANPMSTPHEI ESQLAAAGAT VVITESMAAD KLPSHSHGAL TVVLIDERRD GCLHFWDDLM 190 200 210 220 230 240 SEDEASPLAG DEDDEKVFDP DDVVALPYSS GTTGLPKGVM LTHRSLSTSV AQQVDGENPN 250 260 270 280 290 300 IGLHAGDVIL CALPMFHIYS LNTIMMCGLR VGAAIVVMRR FDLAAMMDLV ERHRVTIAPL 310 320 330 340 350 360 VPPIVVAVAK SEAAAARDLS SVRMVLSGAA PMGKDIEDAF MAKLPGAVLG QGYGMTEAGP 370 380 390 400 410 420 VLSMCLAFAK EPFKVKSGAC GTVVRNAELK IIDPDTGKSL GRNLPGEICI RGQQIMKGYL 430 440 450 460 470 480 NNPEATKNTI DAEGWLHTGD IGYVDDDDEI FIVDRLKEII KYRGFQVAPA ELEALLITHP 490 500 510 520 530 540 SIADAAVVGK QIEPEIGEIP VAFVAKTEGS ELSEDDVKQF VAKEVIYYKK IREVFFVDKI 550 560 PKAPSGKILR KELRKQLQHL QQEA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of rice 4-coumarate:CoA ligase gene, 4-CL.1." Zhao Y., Kung S.D., Dube S.K. Nucleic Acids Res. 18:6144-6144(1990) [PubMed: 2235510] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[5]	"Genome-wide analysis of a land plant-specific acyl:coenzyme A synthetase (ACS) gene family in Arabidopsis, poplar, rice and Physcomitrella." de Azevedo Souza C., Barbazuk B., Ralph S.G., Bohlmann J., Hamberger B., Douglas C.J. New Phytol. 179:987-1003(2008) [PubMed: 18627494] [Abstract] Cited for: GENE FAMILY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X52623 Genomic DNA. Translation: CAA36850.1. Sequence problems. AP003859 Genomic DNA. Translation: BAD05189.1. Different initiation. AP008214 Genomic DNA. Translation: BAF23267.1. AK069932 mRNA. No translation available.
PIR	JU0311.
RefSeq	NP_001061353.1.
UniGene	Os.52921
3D structure databases
SMR	P17814. Positions 20-556.
ModBase	Search...
Genome annotation databases
GeneID	4345054.
GenomeReviews	Gene locus 4CL1 in contig AP008214_GR.
KEGG	osa:4345054.
Organism-specific databases
Gramene	P17814.
Phylogenomic databases
HOGENOM	HBG547964.
Family and domain databases
InterPro	IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

4CL1_ORYSJ

Accession

Primary (citable) accession number: P17814
Secondary accession number(s): Q0J6Z8, Q6ZKV9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	September 27, 2005
Last modified:	January 19, 2010
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)