Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P17814

- 4CL1_ORYSJ

UniProt

P17814 - 4CL1_ORYSJ

Protein

Probable 4-coumarate--CoA ligase 1

Gene

4CL1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 2 (27 Sep 2005)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    ATP + 4-coumarate + CoA = AMP + diphosphate + 4-coumaroyl-CoA.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei440 – 4401ATPBy similarity
    Binding sitei455 – 4551ATPBy similarity
    Binding sitei547 – 5471ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi209 – 2179ATPBy similarity
    Nucleotide bindingi352 – 3576ATPBy similarity

    GO - Molecular functioni

    1. 4-coumarate-CoA ligase activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. phenylpropanoid metabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Phenylpropanoid metabolism

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00372; UER00547.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable 4-coumarate--CoA ligase 1 (EC:6.2.1.12)
    Short name:
    4CL 1
    Short name:
    Os4CL1
    Alternative name(s):
    4-coumaroyl-CoA synthase 1
    Gene namesi
    Name:4CL1
    Synonyms:4CL
    Ordered Locus Names:Os08g0245200, LOC_Os08g14760
    ORF Names:OJ1033_B09.16
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 8

    Organism-specific databases

    GrameneiP17814.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 564564Probable 4-coumarate--CoA ligase 1PRO_0000193031Add
    BLAST

    Proteomic databases

    PRIDEiP17814.

    Expressioni

    Inductioni

    By fungal elicitor and UV irradiation.

    Structurei

    3D structure databases

    ProteinModelPortaliP17814.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni282 – 35170SBD1Add
    BLAST
    Regioni352 – 41968SBD2Add
    BLAST

    Domaini

    Both substrate-binding domains (SBD1 and SBD2) are involved in the substrate recognition, and are sufficient to confer the substrate specificity.

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0318.
    HOGENOMiHOG000230009.
    KOiK01904.
    OMAiAGTNKID.

    Family and domain databases

    InterProiIPR025110. AMP-bd_C.
    IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view]
    PfamiPF00501. AMP-binding. 1 hit.
    PF13193. AMP-binding_C. 1 hit.
    [Graphical view]
    PROSITEiPS00455. AMP_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P17814-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGSMEQQQPE SAAPATEASP EIIFRSKLQD IAITNTLPLH RYCFERLPEV    50
    AARPCLIDGA TGGVLTYADV DRLSRRLAAA LRRAPLGLRR GGVVMSLLRN 100
    SPEFVLSFFA ASRVGAAVTT ANPMSTPHEI ESQLAAAGAT VVITESMAAD 150
    KLPSHSHGAL TVVLIDERRD GCLHFWDDLM SEDEASPLAG DEDDEKVFDP 200
    DDVVALPYSS GTTGLPKGVM LTHRSLSTSV AQQVDGENPN IGLHAGDVIL 250
    CALPMFHIYS LNTIMMCGLR VGAAIVVMRR FDLAAMMDLV ERHRVTIAPL 300
    VPPIVVAVAK SEAAAARDLS SVRMVLSGAA PMGKDIEDAF MAKLPGAVLG 350
    QGYGMTEAGP VLSMCLAFAK EPFKVKSGAC GTVVRNAELK IIDPDTGKSL 400
    GRNLPGEICI RGQQIMKGYL NNPEATKNTI DAEGWLHTGD IGYVDDDDEI 450
    FIVDRLKEII KYRGFQVAPA ELEALLITHP SIADAAVVGK QIEPEIGEIP 500
    VAFVAKTEGS ELSEDDVKQF VAKEVIYYKK IREVFFVDKI PKAPSGKILR 550
    KELRKQLQHL QQEA 564
    Length:564
    Mass (Da):60,902
    Last modified:September 27, 2005 - v2
    Checksum:i9725A4B4A72F8C6E
    GO

    Sequence cautioni

    The sequence CAA36850.1 differs from that shown. Reason: Frameshift at position 496.
    The sequence BAD05189.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAA36850.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti405 – 4051P → R in CAA36850. (PubMed:2235510)Curated
    Sequence conflicti477 – 4771I → N in CAA36850. (PubMed:2235510)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X52623 Genomic DNA. Translation: CAA36850.1. Sequence problems.
    AP003859 Genomic DNA. Translation: BAD05189.1. Different initiation.
    AP008214 Genomic DNA. Translation: BAF23267.1.
    AK069932 mRNA. No translation available.
    PIRiJU0311.
    RefSeqiNP_001061353.1. NM_001067888.1.
    UniGeneiOs.52921.

    Genome annotation databases

    EnsemblPlantsiOS08T0245200-01; OS08T0245200-01; OS08G0245200.
    GeneIDi4345054.
    KEGGiosa:4345054.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X52623 Genomic DNA. Translation: CAA36850.1 . Sequence problems.
    AP003859 Genomic DNA. Translation: BAD05189.1 . Different initiation.
    AP008214 Genomic DNA. Translation: BAF23267.1 .
    AK069932 mRNA. No translation available.
    PIRi JU0311.
    RefSeqi NP_001061353.1. NM_001067888.1.
    UniGenei Os.52921.

    3D structure databases

    ProteinModelPortali P17814.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P17814.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS08T0245200-01 ; OS08T0245200-01 ; OS08G0245200 .
    GeneIDi 4345054.
    KEGGi osa:4345054.

    Organism-specific databases

    Gramenei P17814.

    Phylogenomic databases

    eggNOGi COG0318.
    HOGENOMi HOG000230009.
    KOi K01904.
    OMAi AGTNKID.

    Enzyme and pathway databases

    UniPathwayi UPA00372 ; UER00547 .

    Family and domain databases

    InterProi IPR025110. AMP-bd_C.
    IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view ]
    Pfami PF00501. AMP-binding. 1 hit.
    PF13193. AMP-binding_C. 1 hit.
    [Graphical view ]
    PROSITEi PS00455. AMP_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of rice 4-coumarate:CoA ligase gene, 4-CL.1."
      Zhao Y., Kung S.D., Dube S.K.
      Nucleic Acids Res. 18:6144-6144(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    3. "The rice annotation project database (RAP-DB): 2008 update."
      The rice annotation project (RAP)
      Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: cv. Nipponbare.
    4. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
      The rice full-length cDNA consortium
      Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Nipponbare.
    5. "Genome-wide analysis of a land plant-specific acyl:coenzyme A synthetase (ACS) gene family in Arabidopsis, poplar, rice and Physcomitrella."
      de Azevedo Souza C., Barbazuk B., Ralph S.G., Bohlmann J., Hamberger B., Douglas C.J.
      New Phytol. 179:987-1003(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY.

    Entry informationi

    Entry namei4CL1_ORYSJ
    AccessioniPrimary (citable) accession number: P17814
    Secondary accession number(s): Q0J6Z8, Q6ZKV9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: September 27, 2005
    Last modified: October 1, 2014
    This is version 94 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3