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Protein

Probable 4-coumarate--CoA ligase 1

Gene

4CL1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + 4-coumarate + CoA = AMP + diphosphate + 4-coumaroyl-CoA.

Pathwayi: 3,4',5-trihydroxystilbene biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 3,4',5-trihydroxystilbene from trans-4-coumarate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Probable 4-coumarate--CoA ligase 5 (4CL5), Probable 4-coumarate--CoA ligase 4 (4CL4), Probable 4-coumarate--CoA ligase 1 (4CL1), Probable 4-coumarate--CoA ligase 2 (4CL2), Probable 4-coumarate--CoA ligase 3 (4CL3)
  2. no protein annotated in this organism
This subpathway is part of the pathway 3,4',5-trihydroxystilbene biosynthesis, which is itself part of Phytoalexin biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 3,4',5-trihydroxystilbene from trans-4-coumarate, the pathway 3,4',5-trihydroxystilbene biosynthesis and in Phytoalexin biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei440ATPBy similarity1
Binding sitei455ATPBy similarity1
Binding sitei547ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi209 – 217ATPBy similarity9
Nucleotide bindingi352 – 357ATPBy similarity6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Phenylpropanoid metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.2.1.12. 4460.
ReactomeiR-OSA-75105. Fatty Acyl-CoA Biosynthesis.
UniPathwayiUPA00372; UER00547.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable 4-coumarate--CoA ligase 1 (EC:6.2.1.12)
Short name:
4CL 1
Short name:
Os4CL1
Alternative name(s):
4-coumaroyl-CoA synthase 1
Gene namesi
Name:4CL1
Synonyms:4CL
Ordered Locus Names:Os08g0245200, LOC_Os08g14760
ORF Names:OJ1033_B09.16
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 8

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001930311 – 564Probable 4-coumarate--CoA ligase 1Add BLAST564

Proteomic databases

PaxDbiP17814.
PRIDEiP17814.

Expressioni

Inductioni

By fungal elicitor and UV irradiation.

Gene expression databases

GenevisibleiP17814. OS.

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os08g14760.1.

Structurei

3D structure databases

ProteinModelPortaliP17814.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni282 – 351SBD1Add BLAST70
Regioni352 – 419SBD2Add BLAST68

Domaini

Both substrate-binding domains (SBD1 and SBD2) are involved in the substrate recognition, and are sufficient to confer the substrate specificity.

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1176. Eukaryota.
COG0318. LUCA.
HOGENOMiHOG000230009.
InParanoidiP17814.
KOiK01904.
OMAiAWCAQVG.
OrthoDBiEOG093606MC.

Family and domain databases

InterProiIPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P17814-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSMEQQQPE SAAPATEASP EIIFRSKLQD IAITNTLPLH RYCFERLPEV
60 70 80 90 100
AARPCLIDGA TGGVLTYADV DRLSRRLAAA LRRAPLGLRR GGVVMSLLRN
110 120 130 140 150
SPEFVLSFFA ASRVGAAVTT ANPMSTPHEI ESQLAAAGAT VVITESMAAD
160 170 180 190 200
KLPSHSHGAL TVVLIDERRD GCLHFWDDLM SEDEASPLAG DEDDEKVFDP
210 220 230 240 250
DDVVALPYSS GTTGLPKGVM LTHRSLSTSV AQQVDGENPN IGLHAGDVIL
260 270 280 290 300
CALPMFHIYS LNTIMMCGLR VGAAIVVMRR FDLAAMMDLV ERHRVTIAPL
310 320 330 340 350
VPPIVVAVAK SEAAAARDLS SVRMVLSGAA PMGKDIEDAF MAKLPGAVLG
360 370 380 390 400
QGYGMTEAGP VLSMCLAFAK EPFKVKSGAC GTVVRNAELK IIDPDTGKSL
410 420 430 440 450
GRNLPGEICI RGQQIMKGYL NNPEATKNTI DAEGWLHTGD IGYVDDDDEI
460 470 480 490 500
FIVDRLKEII KYRGFQVAPA ELEALLITHP SIADAAVVGK QIEPEIGEIP
510 520 530 540 550
VAFVAKTEGS ELSEDDVKQF VAKEVIYYKK IREVFFVDKI PKAPSGKILR
560
KELRKQLQHL QQEA
Length:564
Mass (Da):60,902
Last modified:September 27, 2005 - v2
Checksum:i9725A4B4A72F8C6E
GO

Sequence cautioni

The sequence BAD05189 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA36850 differs from that shown. Reason: Frameshift at position 496.Curated
The sequence CAA36850 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti405P → R in CAA36850 (PubMed:2235510).Curated1
Sequence conflicti477I → N in CAA36850 (PubMed:2235510).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52623 Genomic DNA. Translation: CAA36850.1. Sequence problems.
AP003859 Genomic DNA. Translation: BAD05189.1. Different initiation.
AP008214 Genomic DNA. Translation: BAF23267.1.
AP014964 Genomic DNA. Translation: BAT04523.1.
AK069932 mRNA. Translation: BAG91685.1.
PIRiJU0311.
RefSeqiXP_015650724.1. XM_015795238.1.
UniGeneiOs.52921.

Genome annotation databases

EnsemblPlantsiOS08T0245200-01; OS08T0245200-01; OS08G0245200.
GeneIDi4345054.
GrameneiOS08T0245200-01; OS08T0245200-01; OS08G0245200.
KEGGiosa:4345054.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52623 Genomic DNA. Translation: CAA36850.1. Sequence problems.
AP003859 Genomic DNA. Translation: BAD05189.1. Different initiation.
AP008214 Genomic DNA. Translation: BAF23267.1.
AP014964 Genomic DNA. Translation: BAT04523.1.
AK069932 mRNA. Translation: BAG91685.1.
PIRiJU0311.
RefSeqiXP_015650724.1. XM_015795238.1.
UniGeneiOs.52921.

3D structure databases

ProteinModelPortaliP17814.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os08g14760.1.

Proteomic databases

PaxDbiP17814.
PRIDEiP17814.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS08T0245200-01; OS08T0245200-01; OS08G0245200.
GeneIDi4345054.
GrameneiOS08T0245200-01; OS08T0245200-01; OS08G0245200.
KEGGiosa:4345054.

Phylogenomic databases

eggNOGiKOG1176. Eukaryota.
COG0318. LUCA.
HOGENOMiHOG000230009.
InParanoidiP17814.
KOiK01904.
OMAiAWCAQVG.
OrthoDBiEOG093606MC.

Enzyme and pathway databases

UniPathwayiUPA00372; UER00547.
BRENDAi6.2.1.12. 4460.
ReactomeiR-OSA-75105. Fatty Acyl-CoA Biosynthesis.

Gene expression databases

GenevisibleiP17814. OS.

Family and domain databases

InterProiIPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry namei4CL1_ORYSJ
AccessioniPrimary (citable) accession number: P17814
Secondary accession number(s): B7EH38, Q0J6Z8, Q6ZKV9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: September 27, 2005
Last modified: November 30, 2016
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.