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Protein

Probable 4-coumarate--CoA ligase 1

Gene

4CL1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + 4-coumarate + CoA = AMP + diphosphate + 4-coumaroyl-CoA.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei440 – 4401ATPBy similarity
Binding sitei455 – 4551ATPBy similarity
Binding sitei547 – 5471ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi209 – 2179ATPBy similarity
Nucleotide bindingi352 – 3576ATPBy similarity

GO - Molecular functioni

  1. 4-coumarate-CoA ligase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. phenylpropanoid metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Phenylpropanoid metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.2.1.12. 4460.
UniPathwayiUPA00372; UER00547.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable 4-coumarate--CoA ligase 1 (EC:6.2.1.12)
Short name:
4CL 1
Short name:
Os4CL1
Alternative name(s):
4-coumaroyl-CoA synthase 1
Gene namesi
Name:4CL1
Synonyms:4CL
Ordered Locus Names:Os08g0245200, LOC_Os08g14760
ORF Names:OJ1033_B09.16
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763 Componenti: Chromosome 8

Organism-specific databases

GrameneiP17814.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 564564Probable 4-coumarate--CoA ligase 1PRO_0000193031Add
BLAST

Proteomic databases

PRIDEiP17814.

Expressioni

Inductioni

By fungal elicitor and UV irradiation.

Gene expression databases

ExpressionAtlasiP17814. baseline.

Structurei

3D structure databases

ProteinModelPortaliP17814.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni282 – 35170SBD1Add
BLAST
Regioni352 – 41968SBD2Add
BLAST

Domaini

Both substrate-binding domains (SBD1 and SBD2) are involved in the substrate recognition, and are sufficient to confer the substrate specificity.

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0318.
HOGENOMiHOG000230009.
InParanoidiP17814.
KOiK01904.
OMAiIGFVWGE.

Family and domain databases

InterProiIPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P17814-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSMEQQQPE SAAPATEASP EIIFRSKLQD IAITNTLPLH RYCFERLPEV
60 70 80 90 100
AARPCLIDGA TGGVLTYADV DRLSRRLAAA LRRAPLGLRR GGVVMSLLRN
110 120 130 140 150
SPEFVLSFFA ASRVGAAVTT ANPMSTPHEI ESQLAAAGAT VVITESMAAD
160 170 180 190 200
KLPSHSHGAL TVVLIDERRD GCLHFWDDLM SEDEASPLAG DEDDEKVFDP
210 220 230 240 250
DDVVALPYSS GTTGLPKGVM LTHRSLSTSV AQQVDGENPN IGLHAGDVIL
260 270 280 290 300
CALPMFHIYS LNTIMMCGLR VGAAIVVMRR FDLAAMMDLV ERHRVTIAPL
310 320 330 340 350
VPPIVVAVAK SEAAAARDLS SVRMVLSGAA PMGKDIEDAF MAKLPGAVLG
360 370 380 390 400
QGYGMTEAGP VLSMCLAFAK EPFKVKSGAC GTVVRNAELK IIDPDTGKSL
410 420 430 440 450
GRNLPGEICI RGQQIMKGYL NNPEATKNTI DAEGWLHTGD IGYVDDDDEI
460 470 480 490 500
FIVDRLKEII KYRGFQVAPA ELEALLITHP SIADAAVVGK QIEPEIGEIP
510 520 530 540 550
VAFVAKTEGS ELSEDDVKQF VAKEVIYYKK IREVFFVDKI PKAPSGKILR
560
KELRKQLQHL QQEA
Length:564
Mass (Da):60,902
Last modified:September 27, 2005 - v2
Checksum:i9725A4B4A72F8C6E
GO

Sequence cautioni

The sequence BAD05189.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAA36850.1 differs from that shown. Reason: Frameshift at position 496. Curated
The sequence CAA36850.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti405 – 4051P → R in CAA36850 (PubMed:2235510).Curated
Sequence conflicti477 – 4771I → N in CAA36850 (PubMed:2235510).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52623 Genomic DNA. Translation: CAA36850.1. Sequence problems.
AP003859 Genomic DNA. Translation: BAD05189.1. Different initiation.
AP008214 Genomic DNA. Translation: BAF23267.1.
AK069932 mRNA. No translation available.
PIRiJU0311.
RefSeqiNP_001061353.1. NM_001067888.1.
UniGeneiOs.52921.

Genome annotation databases

EnsemblPlantsiOS08T0245200-01; OS08T0245200-01; OS08G0245200.
GeneIDi4345054.
KEGGiosa:4345054.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52623 Genomic DNA. Translation: CAA36850.1. Sequence problems.
AP003859 Genomic DNA. Translation: BAD05189.1. Different initiation.
AP008214 Genomic DNA. Translation: BAF23267.1.
AK069932 mRNA. No translation available.
PIRiJU0311.
RefSeqiNP_001061353.1. NM_001067888.1.
UniGeneiOs.52921.

3D structure databases

ProteinModelPortaliP17814.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP17814.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS08T0245200-01; OS08T0245200-01; OS08G0245200.
GeneIDi4345054.
KEGGiosa:4345054.

Organism-specific databases

GrameneiP17814.

Phylogenomic databases

eggNOGiCOG0318.
HOGENOMiHOG000230009.
InParanoidiP17814.
KOiK01904.
OMAiIGFVWGE.

Enzyme and pathway databases

UniPathwayiUPA00372; UER00547.
BRENDAi6.2.1.12. 4460.

Gene expression databases

ExpressionAtlasiP17814. baseline.

Family and domain databases

InterProiIPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of rice 4-coumarate:CoA ligase gene, 4-CL.1."
    Zhao Y., Kung S.D., Dube S.K.
    Nucleic Acids Res. 18:6144-6144(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  4. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Nipponbare.
  5. "Genome-wide analysis of a land plant-specific acyl:coenzyme A synthetase (ACS) gene family in Arabidopsis, poplar, rice and Physcomitrella."
    de Azevedo Souza C., Barbazuk B., Ralph S.G., Bohlmann J., Hamberger B., Douglas C.J.
    New Phytol. 179:987-1003(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.

Entry informationi

Entry namei4CL1_ORYSJ
AccessioniPrimary (citable) accession number: P17814
Secondary accession number(s): Q0J6Z8, Q6ZKV9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: September 27, 2005
Last modified: April 1, 2015
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.