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Protein

Coagulase/fibrinolysin

Gene

pla

Organism
Yersinia pestis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Seems to play an essential role in plague transmission by mediating flea blockage in a temperature-dependent fashion. Fibrinolytic activity prevails at 37 degrees Celsius whereas coagulase expression predominates at lower temperatures (<30 degrees Celsius). Activates plasminogen by cleaving it.

Catalytic activityi

Converts human Glu-plasminogen to plasmin by cleaving the 560-Arg-|-Val-561 peptide bond that is also hydrolyzed by the mammalian u-plasminogen activator and t-plasminogen activator. Also cleaves arginyl bonds in other proteins.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei104By similarity1
Active sitei106By similarity1
Active sitei226By similarity1
Active sitei228By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Keywords - Biological processi

Blood coagulation, Fibrinolysis, Hemostasis

Enzyme and pathway databases

BRENDAi3.4.23.48. 4559.

Protein family/group databases

MEROPSiA26.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Coagulase/fibrinolysin (EC:3.4.23.48)
Alternative name(s):
Plasminogen activator
Gene namesi
Name:pla
Ordered Locus Names:YPPCP1.07, YP_pPCP08
Encoded oniPlasmid pPCP14 Publications
Plasmid pKYP11 Publication
OrganismiYersinia pestis
Taxonomic identifieri632 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeYersinia
Proteomesi
  • UP000000815 Componenti: Plasmid pPCP1
  • UP000001019 Componenti: Plasmid pPCP1

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1075037.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000002581921 – 312Coagulase/fibrinolysinAdd BLAST292

Structurei

Secondary structure

1312
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi31 – 33Combined sources3
Beta strandi34 – 52Combined sources19
Turni54 – 56Combined sources3
Beta strandi59 – 82Combined sources24
Beta strandi85 – 96Combined sources12
Beta strandi98 – 106Combined sources9
Beta strandi116 – 142Combined sources27
Beta strandi144 – 165Combined sources22
Beta strandi167 – 170Combined sources4
Turni171 – 174Combined sources4
Beta strandi175 – 178Combined sources4
Beta strandi184 – 205Combined sources22
Beta strandi208 – 228Combined sources21
Helixi229 – 231Combined sources3
Beta strandi233 – 256Combined sources24
Beta strandi259 – 270Combined sources12
Beta strandi275 – 280Combined sources6
Turni282 – 284Combined sources3
Helixi292 – 294Combined sources3
Beta strandi295 – 312Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2X4MX-ray2.55A/B/C/D21-312[»]
2X55X-ray1.85A21-312[»]
2X56X-ray2.30A21-312[»]
4DCBX-ray2.03A25-312[»]
ProteinModelPortaliP17811.
SMRiP17811.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP17811.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase A26 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

HOGENOMiHOG000117799.
KOiK08566.
OMAiYNYDNGA.

Family and domain databases

Gene3Di2.40.128.90. 1 hit.
InterProiIPR020080. OM_adhesin/peptidase_omptin.
IPR023619. Peptidase_A26.
IPR020079. Peptidase_A26_CS.
IPR000036. Peptidase_A26_omptin.
[Graphical view]
PfamiPF01278. Omptin. 1 hit.
[Graphical view]
PIRSFiPIRSF001522. Peptidase_A26. 1 hit.
PRINTSiPR00482. OMPTIN.
SUPFAMiSSF69917. SSF69917. 1 hit.
PROSITEiPS00834. OMPTIN_1. 1 hit.
PS00835. OMPTIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17811-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKSSIVATI ITILSGSANA ASSQLIPNIS PDSFTVAAST GMLSGKSHEM
60 70 80 90 100
LYDAETGRKI SQLDWKIKNV AILKGDISWD PYSFLTLNAR GWTSLASGSG
110 120 130 140 150
NMDDYDWMNE NQSEWTDHSS HPATNVNHAN EYDLNVKGWL LQDENYKAGI
160 170 180 190 200
TAGYQETRFS WTATGGSYSY NNGAYTGNFP KGVRVIGYNQ RFSMPYIGLA
210 220 230 240 250
GQYRINDFEL NALFKFSDWV RAHDNDEHYM RDLTFREKTS GSRYYGTVIN
260 270 280 290 300
AGYYVTPNAK VFAEFTYSKY DEGKGGTQTI DKNSGDSVSI GGDAAGISNK
310
NYTVTAGLQY RF
Length:312
Mass (Da):34,611
Last modified:August 1, 1990 - v1
Checksum:i52B7CB4F32E8FBE9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti279T → I in AAS58765 (PubMed:15368893).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15136 Genomic DNA. Translation: CAA33235.1.
M27820 Genomic DNA. Translation: AAA27667.1. Sequence problems.
AF053945 Genomic DNA. Translation: AAC62545.1.
AL109969 Genomic DNA. Translation: CAB53170.1.
AE017046 Genomic DNA. Translation: AAS58765.1.
PIRiA42928.
S06979. A30916.
RefSeqiNP_395233.1. NC_003132.1.
NP_857784.1. NC_004837.1.
WP_002218234.1. NZ_LQAX01000176.1.
YP_006960975.1. NC_019235.1.

Genome annotation databases

EnsemblBacteriaiAAS58765; AAS58765; YP_pPCP08.
GeneIDi1172643.
13917718.
15151870.
KEGGiype:YPPCP1.07.
ypm:YP_pPCP08.
PATRICi18588745. VBIYerPes7843_0117.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15136 Genomic DNA. Translation: CAA33235.1.
M27820 Genomic DNA. Translation: AAA27667.1. Sequence problems.
AF053945 Genomic DNA. Translation: AAC62545.1.
AL109969 Genomic DNA. Translation: CAB53170.1.
AE017046 Genomic DNA. Translation: AAS58765.1.
PIRiA42928.
S06979. A30916.
RefSeqiNP_395233.1. NC_003132.1.
NP_857784.1. NC_004837.1.
WP_002218234.1. NZ_LQAX01000176.1.
YP_006960975.1. NC_019235.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2X4MX-ray2.55A/B/C/D21-312[»]
2X55X-ray1.85A21-312[»]
2X56X-ray2.30A21-312[»]
4DCBX-ray2.03A25-312[»]
ProteinModelPortaliP17811.
SMRiP17811.
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

ChEMBLiCHEMBL1075037.

Protein family/group databases

MEROPSiA26.003.

Protocols and materials databases

DNASUi1149148.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAS58765; AAS58765; YP_pPCP08.
GeneIDi1172643.
13917718.
15151870.
KEGGiype:YPPCP1.07.
ypm:YP_pPCP08.
PATRICi18588745. VBIYerPes7843_0117.

Phylogenomic databases

HOGENOMiHOG000117799.
KOiK08566.
OMAiYNYDNGA.

Enzyme and pathway databases

BRENDAi3.4.23.48. 4559.

Miscellaneous databases

EvolutionaryTraceiP17811.
PROiP17811.

Family and domain databases

Gene3Di2.40.128.90. 1 hit.
InterProiIPR020080. OM_adhesin/peptidase_omptin.
IPR023619. Peptidase_A26.
IPR020079. Peptidase_A26_CS.
IPR000036. Peptidase_A26_omptin.
[Graphical view]
PfamiPF01278. Omptin. 1 hit.
[Graphical view]
PIRSFiPIRSF001522. Peptidase_A26. 1 hit.
PRINTSiPR00482. OMPTIN.
SUPFAMiSSF69917. SSF69917. 1 hit.
PROSITEiPS00834. OMPTIN_1. 1 hit.
PS00835. OMPTIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCOLY_YERPE
AccessioniPrimary (citable) accession number: P17811
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: November 30, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Plasmid, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.